Restriction map for
pFN21AE4436
(Circular) (Six-base) MAPSORT of: pFN21AE4436.seq Check: 666 from: 1 to: 6064
pFN21AE4436 6064 bp
With 182 enzymes: SgfI *
March 28, 2012 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3407 1937
Size: 1470 4594
Acc65I G'GTAC_C
Cuts at: 2386 3104 3385 2386
Size: 718 281 5065
Fragments arranged by size:
5065 718 281
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2152 2493 2738 4127 5511 5779 1632
Size: 520 341 245 1389 1384 268 1917
Fragments arranged by size:
1917 1389 1384 520 341 268 245
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 2735 4963 5940 1203
Size: 1532 2228 977 1327
Fragments arranged by size:
2228 1532 1327 977
AgeI A'CCGG_T
Cuts at: 2560 4895 2560
Size: 2335 3729
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2675 2721 1184
Size: 1491 46 4527
Fragments arranged by size:
4527 1491 46
AlwNI CAG_nnn'CTG
Cuts at: 2246 2648 2716 3807 5379 2246
Size: 402 68 1091 1572 2931
Fragments arranged by size:
2931 1572 1091 402 68
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3019 3373 3599 3751 5860 1493
Size: 1526 354 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 354 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 3389 1870
Size: 73 88 1358 4545
Fragments arranged by size:
4545 1358 88 73
BamHI G'GATC_C
Cuts at: 3337 3394 3761 3337
Size: 57 367 5640
Fragments arranged by size:
5640 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 2563 3104 3385 4840
Size: 325 217 627 599 177 541 281 1455
Cuts at: 4840 618
Size: 1842
Fragments arranged by size:
1842 1455 627 599 541 325 281 217
177
BanII G_rGCy'C
Cuts at: 1391 2797 3383 1391
Size: 1406 586 4072
Fragments arranged by size:
4072 1406 586
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2158 928
Size: 971 259 4834
Fragments arranged by size:
4834 971 259
BbvCI CC'TCA_GC
Cuts at: 2866 2866
Size: 6064
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4255 4289 1903
Size: 34 50 34 2234 34 3678
Fragments arranged by size:
3678 2234 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 3271 1548
Size: 1723 4341
BfrBI ATG'CAT
Cuts at: 3193 3193
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2468 4675 136
Size: 107 122 71 631 605 796 2207 1525
Fragments arranged by size:
2207 1525 796 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 2637 2709 3481 2637
Size: 72 772 5220
Fragments arranged by size:
5220 772 72
Bme1580I G_kGCm'C
Cuts at: 1790 2568 4125 5281 1790
Size: 778 1557 1156 2573
Fragments arranged by size:
2573 1557 1156 778
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3062 3795 4753
Size: 416 53 83 399 24 1645 733 958
Cuts at: 4753 442
Size: 1753
Fragments arranged by size:
1753 1645 958 733 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3368
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4724 1239
Size: 3485 2579
Bpu10I CC'TnA_GC
Cuts at: 2457 2866 2457
Size: 409 5655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5054 5352 4189
Size: 865 298 4901
Fragments arranged by size:
4901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2110 4727 882
Size: 617 611 2617 2219
Fragments arranged by size:
2617 2219 617 611
BsaAI yAC'GTr
Cuts at: 493 1204 3256 3855 493
Size: 711 2052 599 2702
Fragments arranged by size:
2702 2052 711 599
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 4253 4888 275
Size: 53 83 186 936 2338 382 635 1451
Fragments arranged by size:
2338 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2560 4497 4895 5169 5316 5727 2560
Size: 1937 398 274 147 411 2897
Fragments arranged by size:
2897 1937 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2277 2307 3216 3246 1178
Size: 30 165 30 874 30 909 30 3996
Fragments arranged by size:
3996 909 874 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3341 1186
Size: 2155 3909
BseYI C'CCAG_C
Cuts at: 2230 5267 5763 2230
Size: 3037 496 2531
Fragments arranged by size:
3037 2531 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 1163
Size: 573 627 4864
Fragments arranged by size:
4864 627 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4125 4210 5281 4125
Size: 85 1071 4908
Fragments arranged by size:
4908 1071 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
BsmBI CGTCTCn'nnnn_
Cuts at: 2080 2080
Size: 6064
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2568 2797 3383 4125 4210 5281 1391
Size: 399 778 229 586 742 85 1071 2174
Fragments arranged by size:
2174 1071 778 742 586 399 229 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2335 3419 844
Size: 1491 1084 3489
Fragments arranged by size:
3489 1491 1084
BsrBI CCG'CTC
Cuts at: 1394 1823 2046 3951 1394
Size: 429 223 1905 3507
Fragments arranged by size:
3507 1905 429 223
BsrDI GCAATG_nn'
Cuts at: 65 1248 2703 2748 3127 4559 4733 65
Size: 1183 1455 45 379 1432 174 1396
Fragments arranged by size:
1455 1432 1396 1183 379 174 45
BsrFI r'CCGG_y
Cuts at: 1700 1810 2467 2560 3276 4708 4895 1700
Size: 110 657 93 716 1432 187 2869
Fragments arranged by size:
2869 1432 716 657 187 110 93
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6064
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 2126 1184
Size: 942 5122
BstXI CCAn_nnnn'nTGG
Cuts at: 2929 2929
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 3337 3358 3394 3761 4146 4163 5615 5701
Size: 1365 21 36 367 385 17 1452 86
Cuts at: 5701 5713 6059 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1365 385 367 346 86 36
21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2975 513
Size: 552 1910 3602
Fragments arranged by size:
3602 1910 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 2910 2970 3580 4374 4394 2910
Size: 60 610 794 20 4580
Fragments arranged by size:
4580 794 610 60 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2967 3511 4400
Size: 54 1425 50 275 18 1137 544 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1137 889 544 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3994 1353
Size: 2641 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 4651 5025 5171 387
Size: 1064 138 455 2607 374 146 1280
Fragments arranged by size:
2607 1280 1064 455 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2152 2493 2738 4127 4724 5511 5779
Size: 393 520 341 245 1389 597 787 268
Cuts at: 5779 1239
Size: 1524
Fragments arranged by size:
1524 1389 787 597 520 393 341 268
245
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 6064
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2362 2394 2779
Size: 32 135 32 749 32 9 32 385
Cuts at: 2779 2811 3256 3288 4705 4737 4779 4811 1373
Size: 32 445 32 1417 32 42 32 2626
Fragments arranged by size:
2626 1417 749 445 385 135 42 32
32 32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KpnI G_GTAC'C
Cuts at: 2390 3108 3389 2390
Size: 718 281 5065
Fragments arranged by size:
5065 718 281
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2263 5178 5362 1305
Size: 958 2915 184 2007
Fragments arranged by size:
2915 2007 958 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2969 10
Size: 54 1425 343 1137 3105
Fragments arranged by size:
3105 1425 1137 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 4022 4381 4540 518
Size: 648 1386 1470 359 159 2042
Fragments arranged by size:
2042 1470 1386 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2195 2516 3329 3480 4157 5305 5550
Size: 416 173 321 813 151 677 1148 245
Cuts at: 5550 1606
Size: 2120
Fragments arranged by size:
2120 1148 813 677 416 321 245 173
151
NaeI GCC'GGC
Cuts at: 2469 2469
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5512
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NgoMIV G'CCGG_C
Cuts at: 2467 2467
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NsiI A_TGCA'T
Cuts at: 3195 3195
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2739 3422 4967 1132
Size: 97 1510 683 1545 2229
Fragments arranged by size:
2229 1545 1510 683 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 2735 4963 2735
Size: 2228 3836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2363 2648 1108
Size: 1255 285 4524
Fragments arranged by size:
4524 1255 285
PfoI T'CCnGG_A
Cuts at: 2781 3354 2781
Size: 573 5491
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2491 2536 2608 3416 830
Size: 1661 45 72 808 3478
Fragments arranged by size:
3478 1661 808 72 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2195 2195
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SexAI A'CCwGG_T
Cuts at: 2122 2122
Size: 6064
SfcI C'TryA_G
Cuts at: 826 1045 2190 2487 2508 2532 2604 3219 3412
Size: 219 1145 297 21 24 72 615 193
Cuts at: 3412 4547 5228 5419 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1145 1135 681 615 297 219 193
191 72 24 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 2560 1700
Size: 860 5204
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4168 5069 5331 820
Size: 197 926 2225 901 262 1553
Fragments arranged by size:
2225 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 2864 1517
Size: 1347 4717
StyI C'CwwG_G
Cuts at: 513 1065 3503 513
Size: 552 2438 3074
Fragments arranged by size:
3074 2438 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2554 3169 4091 4108 4261 4446 4891
Size: 1255 974 615 922 17 153 185 445
Cuts at: 4891 325
Size: 1498
Fragments arranged by size:
1498 1255 974 922 615 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2428 2460 6024 6056 2428
Size: 32 3564 32 2436
Fragments arranged by size:
3564 2436 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3049 3091 3253 3049
Size: 42 162 5860
Fragments arranged by size:
5860 162 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NarI NruI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI