Restriction map for
pFN21AE4434
(Circular) (Six-base) MAPSORT of: pFN21AE4434.seq Check: 1979 from: 1 to: 6061
pFN21AE4434 6061 bp
With 182 enzymes: SgfI *
March 28, 2012 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3871 278
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2834 3404 1937
Size: 897 570 4594
Fragments arranged by size:
4594 897 570
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2606 4124 5508 5776 1632
Size: 974 1518 1384 268 1917
Fragments arranged by size:
1917 1518 1384 974 268
AflII C'TTAA_G
Cuts at: 820 1017 3293 820
Size: 197 2276 3588
Fragments arranged by size:
3588 2276 197
AflIII A'CryG_T
Cuts at: 1203 2872 4960 5937 1203
Size: 1669 2088 977 1327
Fragments arranged by size:
2088 1669 1327 977
AgeI A'CCGG_T
Cuts at: 4892 4892
Size: 6061
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6061
AlwNI CAG_nnn'CTG
Cuts at: 2704 3804 5376 2704
Size: 1100 1572 3389
Fragments arranged by size:
3389 1572 1100
ApaLI G'TGCA_C
Cuts at: 2940 4118 5274 2940
Size: 1178 1156 3727
Fragments arranged by size:
3727 1178 1156
ApoI r'AATT_y
Cuts at: 1493 2237 3370 3596 3748 5857 1493
Size: 744 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 2757 3386 1870
Size: 73 586 228 629 4545
Fragments arranged by size:
4545 629 586 228 73
BamHI G'GATC_C
Cuts at: 2819 3391 3758 2819
Size: 572 367 5122
Fragments arranged by size:
5122 572 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2312 3382 4837 618
Size: 325 217 627 315 210 1070 1455 1842
Fragments arranged by size:
1842 1455 1070 627 325 315 217 210
BanII G_rGCy'C
Cuts at: 1391 3003 3045 3380 1391
Size: 1612 42 335 4072
Fragments arranged by size:
4072 1612 335 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2694 2782 3122 928
Size: 971 189 606 88 340 3867
Fragments arranged by size:
3867 971 606 340 189 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4252 4286 1903
Size: 34 2315 34 3678
Fragments arranged by size:
3678 2315 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4672 136
Size: 107 122 71 631 605 3000 1525
Fragments arranged by size:
3000 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2292 6056 2292
Size: 3764 2297
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2361 2570 2880 2944 3346 4122 5278 1790
Size: 571 209 310 64 402 776 1156 2573
Fragments arranged by size:
2573 1156 776 571 402 310 209 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2899 3792 4750
Size: 416 53 83 399 24 1482 893 958
Cuts at: 4750 442
Size: 1753
Fragments arranged by size:
1753 1482 958 893 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3125 3131 3332 4721 1239
Size: 1886 6 201 1389 2579
Fragments arranged by size:
2579 1886 1389 201 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2317 4186 5051 5349 2317
Size: 1869 865 298 3029
Fragments arranged by size:
3029 1869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4724 882
Size: 617 3225 2219
Fragments arranged by size:
3225 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3166 3852 493
Size: 711 1962 686 2702
Fragments arranged by size:
2702 1962 711 686
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3868 4250 4885 275
Size: 53 83 186 936 2335 382 635 1451
Fragments arranged by size:
2335 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4494 4892 5166 5313 5724 4494
Size: 398 274 147 411 4831
Fragments arranged by size:
4831 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5836
Fragments arranged by size:
5836 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 1186
Size: 1000 5061
BseYI C'CCAG_C
Cuts at: 2679 3038 3285 5264 5760 2679
Size: 359 247 1979 496 2980
Fragments arranged by size:
2980 1979 496 359 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2782 1163
Size: 573 1046 4442
Fragments arranged by size:
4442 1046 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2944 4122 4207 5278 2944
Size: 1178 85 1071 3727
Fragments arranged by size:
3727 1178 1071 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2570 2880 2944 3003 3045 3346
Size: 399 571 209 310 64 59 42 301
Cuts at: 3346 3380 4122 4207 5278 1391
Size: 34 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 571 399 310 301 209
85 64 59 42 34
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3416 844
Size: 2572 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2423 3948 1394
Size: 429 600 1525 3507
Fragments arranged by size:
3507 1525 600 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 4556 4730 65
Size: 1183 1245 2063 174 1396
Fragments arranged by size:
2063 1396 1245 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4705 4892 1700
Size: 110 2895 187 2869
Fragments arranged by size:
2895 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 2334 4121 5133 2334
Size: 1787 1012 3262
Fragments arranged by size:
3262 1787 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3035 794
Size: 2241 3820
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 2921 2921
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 2292 2455 2819 3391 3758 4143 4160 5612
Size: 320 163 364 572 367 385 17 1452
Cuts at: 5612 5698 5710 6056 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 572 385 367 364 346 320
163 86 17 12
BstZ17I GTA'TAC
Cuts at: 2835 2835
Size: 6061
BtgI C'CryG_G
Cuts at: 513 1065 2512 513
Size: 552 1447 4062
Fragments arranged by size:
4062 1447 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 2010 2794 3346 3577 4371 4391 2010
Size: 784 552 231 794 20 3680
Fragments arranged by size:
3680 794 784 552 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 2220 2452 3365 2220
Size: 232 913 4916
Fragments arranged by size:
4916 913 232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2587 5068 809
Size: 507 1271 2481 1802
Fragments arranged by size:
2481 1802 1271 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 3508 4397
Size: 54 1425 50 275 18 318 1360 889
Cuts at: 4397 6026 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1360 889 318 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3991 1353
Size: 2638 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2714 4648 5022 5168 387
Size: 1064 138 1125 1934 374 146 1280
Fragments arranged by size:
1934 1280 1125 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2606 3125 3131 3332 4124 4721 5508
Size: 393 974 519 6 201 792 597 787
Cuts at: 5508 5776 1239
Size: 268 1524
Fragments arranged by size:
1524 974 792 787 597 519 393 268
201 6
EcoRI G'AATT_C
Cuts at: 3370 3748 3370
Size: 378 5683
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 5208 1678
Size: 3530 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373
Size: 32 135 32 3130 32 42 32 2626
Fragments arranged by size:
3130 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3405 3651 669
Size: 988 281 1467 246 3079
Fragments arranged by size:
3079 1467 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3092 748
Size: 2344 3717
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2755 5175 5359 1305
Size: 1450 2420 184 2007
Fragments arranged by size:
2420 2007 1450 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2150 10
Size: 54 1425 343 318 3921
Fragments arranged by size:
3921 1425 343 318 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4019 4378 4537 518
Size: 648 2853 359 159 2042
Fragments arranged by size:
2853 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2683 3477 4154 5302 5547 1606
Size: 1077 794 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 794 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2512 513
Size: 552 1447 4062
Fragments arranged by size:
4062 1447 552
NdeI CA'TA_TG
Cuts at: 387 3031 387
Size: 2644 3417
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6061
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2876 3419 4964 1132
Size: 97 1647 543 1545 2229
Fragments arranged by size:
2229 1647 1545 543 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6061
PciI A'CATG_T
Cuts at: 2872 4960 2872
Size: 2088 3973
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6061
PfoI T'CCnGG_A
Cuts at: 2019 2019
Size: 6061
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6061
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PspXI vC'TCGA_Gb
Cuts at: 2529 2529
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2709 3413 830
Size: 1879 704 3478
Fragments arranged by size:
3478 1879 704
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2683 2683
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 3403 1936
Size: 1467 4594
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 2651 3071 4309 6021 2651
Size: 420 1238 1712 2691
Fragments arranged by size:
2691 1712 1238 420
SfcI C'TryA_G
Cuts at: 826 1045 2705 3409 4544 5225 5416 826
Size: 219 1660 704 1135 681 191 1471
Fragments arranged by size:
1660 1471 1135 704 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6061
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2296 2529 3293 4165 5066 5328
Size: 197 926 353 233 764 872 901 262
Cuts at: 5328 820
Size: 1553
Fragments arranged by size:
1553 926 901 872 764 353 262 233
197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 3419 1132
Size: 2287 3774
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6061
StyI C'CwwG_G
Cuts at: 513 1065 2512 3189 3500 513
Size: 552 1447 677 311 3074
Fragments arranged by size:
3074 1447 677 552 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2261 2759 2927 3272 4088 4105 4258
Size: 1255 681 498 168 345 816 17 153
Cuts at: 4258 4443 4888 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 816 681 498 445 345 185
168 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2619 2649 2805
Size: 274 80 33 51 1443 642 30 156
Cuts at: 2805 3069 4307 6019 96
Size: 264 1238 1712 138
Fragments arranged by size:
1712 1443 1238 642 274 264 156 138
80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6021 6053 6021
Size: 32 6029
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 2204 3397 2204
Size: 1193 4868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2383 2530 2017
Size: 366 147 5548
Fragments arranged by size:
5548 366 147
XhoI C'TCGA_G
Cuts at: 1943 2529 1943
Size: 586 5475
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 4190 4190
Size: 6061
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3869 276
Size: 53 83 186 936 2335 2468
Fragments arranged by size:
2468 2335 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI