Restriction map for
pFN21AE4427
(Circular) (Six-base) MAPSORT of: pFN21AE4427.seq Check: 596 from: 1 to: 6037
pFN21AE4427 6037 bp
With 182 enzymes: SgfI *
March 28, 2012 14:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2471 3847 278
Size: 53 83 186 936 935 1376 2468
Fragments arranged by size:
2468 1376 936 935 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3380 1937
Size: 1443 4594
Acc65I G'GTAC_C
Cuts at: 3358 3358
Size: 6037
AclI AA'CG_TT
Cuts at: 1563 4164 4537 1563
Size: 2601 373 3063
Fragments arranged by size:
3063 2601 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2548 2636 4100 5484 5752 1632
Size: 916 88 1464 1384 268 1917
Fragments arranged by size:
1917 1464 1384 916 268 88
AfeI AGC'GCT
Cuts at: 2087 2087
Size: 6037
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5840
AflIII A'CryG_T
Cuts at: 1203 4936 5913 1203
Size: 3733 977 1327
Fragments arranged by size:
3733 1327 977
AgeI A'CCGG_T
Cuts at: 4868 4868
Size: 6037
AhdI GACnn_n'nnGTC
Cuts at: 4766 4766
Size: 6037
AleI CACnn'nnGTG
Cuts at: 1166 2933 1166
Size: 1767 4270
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6037
AlwNI CAG_nnn'CTG
Cuts at: 3780 5352 3780
Size: 1572 4465
ApaI G_GGCC'C
Cuts at: 2626 2817 2626
Size: 191 5846
ApaLI G'TGCA_C
Cuts at: 4094 5250 4094
Size: 1156 4881
ApoI r'AATT_y
Cuts at: 1493 3346 3572 3724 5833 1493
Size: 1853 226 152 2109 1697
Fragments arranged by size:
2109 1853 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4591 160
Size: 872 3559 1606
Fragments arranged by size:
3559 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
AvaI C'yCGr_G
Cuts at: 1870 1943 2172 3362 1870
Size: 73 229 1190 4545
Fragments arranged by size:
4545 1190 229 73
BamHI G'GATC_C
Cuts at: 2209 3367 3734 2209
Size: 1158 367 4512
Fragments arranged by size:
4512 1158 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2550 3358 4813 618
Size: 325 217 627 763 808 1455 1842
Fragments arranged by size:
1842 1455 808 763 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2171 2310 2376 2626 2817 3356 1391
Size: 780 139 66 250 191 539 4072
Fragments arranged by size:
4072 780 539 250 191 139 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2386 2554 928
Size: 971 487 168 4411
Fragments arranged by size:
4411 971 487 168
BbvCI CC'TCA_GC
Cuts at: 2818 2818
Size: 6037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4228 4262 1903
Size: 34 2291 34 3678
Fragments arranged by size:
3678 2291 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3929 5139 3929
Size: 1210 4827
BclI T'GATC_A
Cuts at: 1548 3046 1548
Size: 1498 4539
BfrBI ATG'CAT
Cuts at: 2707 2707
Size: 6037
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4648 136
Size: 107 122 71 631 605 2976 1525
Fragments arranged by size:
2976 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6032 6032
Size: 6037
BlpI GC'TnA_GC
Cuts at: 3454 3454
Size: 6037
Bme1580I G_kGCm'C
Cuts at: 1790 2553 2626 2817 4098 5254 1790
Size: 763 73 191 1281 1156 2573
Fragments arranged by size:
2573 1281 1156 763 191 73
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3076 3768 4726
Size: 416 53 83 399 24 1659 692 958
Cuts at: 4726 442
Size: 1753
Fragments arranged by size:
1753 1659 958 692 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2271 3068 4697 1239
Size: 1032 797 1629 2579
Fragments arranged by size:
2579 1629 1032 797
Bpu10I CC'TnA_GC
Cuts at: 2040 2089 2818 2040
Size: 49 729 5259
Fragments arranged by size:
5259 729 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4162 5027 5325 4162
Size: 865 298 4874
Fragments arranged by size:
4874 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 4700 882
Size: 617 525 2676 2219
Fragments arranged by size:
2676 2219 617 525
BsaAI yAC'GTr
Cuts at: 493 1204 3828 493
Size: 711 2624 2702
Fragments arranged by size:
2702 2624 711
BsaBI GATnn'nnATC
Cuts at: 3324 6031 3324
Size: 2707 3330
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2468 3844 4226 4861
Size: 53 83 186 936 935 1376 382 635
Cuts at: 4861 275
Size: 1451
Fragments arranged by size:
1451 1376 936 935 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3054 4470 4868 5142 5289 5700 3054
Size: 1416 398 274 147 411 3391
Fragments arranged by size:
3391 1416 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5812
Fragments arranged by size:
5812 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2477 1186
Size: 928 363 4746
Fragments arranged by size:
4746 928 363
BseYI C'CCAG_C
Cuts at: 2082 2297 3078 5240 5736 2082
Size: 215 781 2162 496 2383
Fragments arranged by size:
2383 2162 781 496 215
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 2457 2529 3233 1163
Size: 573 663 58 72 704 3967
Fragments arranged by size:
3967 704 663 573 72 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2122 3487 4248 5276 1540
Size: 275 186 121 1365 761 1028 2301
Fragments arranged by size:
2301 1365 1028 761 275 186 121
BsiHKAI G_wGCw'C
Cuts at: 3159 4098 4183 5254 3159
Size: 939 85 1071 3942
Fragments arranged by size:
3942 1071 939 85
BsmI GAATG_Cn'
Cuts at: 3548 3641 3548
Size: 93 5944
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2310 2376 2553 2626 2817 3159
Size: 399 381 139 66 177 73 191 342
Cuts at: 3159 3356 4098 4183 5254 1391
Size: 197 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 381 342 197 191
177 139 85 73 66
BspEI T'CCGG_A
Cuts at: 5700 5700
Size: 6037
BspHI T'CATG_A
Cuts at: 2376 3049 3926 5656 2376
Size: 673 877 1730 2757
Fragments arranged by size:
2757 1730 877 673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2418 3392 844
Size: 1574 974 3489
Fragments arranged by size:
3489 1574 974
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 2365 3924 1394
Size: 429 281 261 1559 3507
Fragments arranged by size:
3507 1559 429 281 261
BsrDI GCAATG_nn'
Cuts at: 65 1248 3103 4532 4706 65
Size: 1183 1855 1429 174 1396
Fragments arranged by size:
1855 1429 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2154 4681 4868 1700
Size: 110 344 2527 187 2869
Fragments arranged by size:
2869 2527 344 187 110
BsrGI T'GTAC_A
Cuts at: 96 2130 96
Size: 2034 4003
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6037
BssSI C'ACGA_G
Cuts at: 4097 5109 4097
Size: 1012 5025
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2422 794
Size: 1628 4409
BstBI TT'CG_AA
Cuts at: 4841 4841
Size: 6037
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6037
BstYI r'GATC_y
Cuts at: 1972 2209 2733 3367 3734 4119 4136 5588 5674
Size: 237 524 634 367 385 17 1452 86
Cuts at: 5674 5686 6032 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 634 524 385 367 346 237
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2205 513
Size: 552 939 30 171 4345
Fragments arranged by size:
4345 939 552 171 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5298
Fragments arranged by size:
5298 683 56
BtsI GCAGTG_nn'
Cuts at: 3553 4347 4367 3553
Size: 794 20 5223
Fragments arranged by size:
5223 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6037
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6002
DraI TTT'AAA
Cuts at: 3341 3341
Size: 6037
DraIII CAC_nnn'GTG
Cuts at: 3199 3199
Size: 6037
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5044 809
Size: 507 3728 1802
Fragments arranged by size:
3728 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2608 3484
Size: 54 1425 50 275 18 192 586 876
Cuts at: 3484 4373 6002 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 876 586 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3484 1537
Size: 275 1672 4090
Fragments arranged by size:
4090 1672 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3967 1353
Size: 2614 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4624 4998 5144 387
Size: 1064 138 3035 374 146 1280
Fragments arranged by size:
3035 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2548 2636 3068 4100 4697 5484
Size: 393 639 277 88 432 1032 597 787
Cuts at: 5484 5752 1239
Size: 268 1524
Fragments arranged by size:
1524 1032 787 639 597 432 393 277
268 88
EcoO109I rG'GnC_Cy
Cuts at: 2045 2239 2622 2623 2814 2045
Size: 194 383 1 191 5268
Fragments arranged by size:
5268 383 194 191 1
EcoRI G'AATT_C
Cuts at: 3346 3724 3346
Size: 378 5659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 6037
FspI TGC'GCA
Cuts at: 4543 4543
Size: 6037
HaeII r_GCGC'y
Cuts at: 1678 2089 5184 1678
Size: 411 3095 2531
Fragments arranged by size:
3095 2531 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2023 2055 3233 3265 4678
Size: 32 135 32 451 32 1178 32 1413
Cuts at: 4678 4710 4752 4784 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1413 1178 451 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3381 3627 669
Size: 988 281 1443 246 3079
Fragments arranged by size:
3079 1443 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6037
HpaI GTT'AAC
Cuts at: 3627 3627
Size: 6037
KpnI G_GTAC'C
Cuts at: 3362 3362
Size: 6037
MfeI C'AATT_G
Cuts at: 3636 3636
Size: 6037
MluI A'CGCG_T
Cuts at: 5913 5913
Size: 6037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5151 5335 1305
Size: 3846 184 2007
Fragments arranged by size:
3846 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2610 10
Size: 54 1425 343 778 3437
Fragments arranged by size:
3437 1425 778 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 2933 3995 4354 4513 518
Size: 648 1536 231 1062 359 159 2042
Fragments arranged by size:
2042 1536 1062 648 359 231 159
MspA1I CmG'CkG
Cuts at: 1606 2036 3453 4130 5278 5523 1606
Size: 430 1417 677 1148 245 2120
Fragments arranged by size:
2120 1417 1148 677 430 245
NaeI GCC'GGC
Cuts at: 2156 2156
Size: 6037
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4546
Fragments arranged by size:
4546 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6037
NgoMIV G'CCGG_C
Cuts at: 2154 2154
Size: 6037
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6037
NotI GC'GGCC_GC
Cuts at: 3484 3484
Size: 6037
NsiI A_TGCA'T
Cuts at: 2709 2709
Size: 6037
NspI r_CATG'y
Cuts at: 1132 1229 2650 2707 3096 3395 4940 1132
Size: 97 1421 57 389 299 1545 2229
Fragments arranged by size:
2229 1545 1421 389 299 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6037
PciI A'CATG_T
Cuts at: 4936 4936
Size: 6037
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2637 3199 1108
Size: 1529 562 3946
Fragments arranged by size:
3946 1529 562
PfoI T'CCnGG_A
Cuts at: 2340 2988 2340
Size: 648 5389
PmeI GTTT'AAAC
Cuts at: 3341 3341
Size: 6037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4106 4138 5619 5651 4106
Size: 32 1481 32 4492
Fragments arranged by size:
4492 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2045 2045
Size: 6037
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6037
PsiI TTA'TAA
Cuts at: 3607 3607
Size: 6037
PspOMI G'GGCC_C
Cuts at: 2622 2813 2622
Size: 191 5846
PstI C_TGCA'G
Cuts at: 830 2538 3389 830
Size: 1708 851 3478
Fragments arranged by size:
3478 1708 851
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6037
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 6037
SalI G'TCGA_C
Cuts at: 1936 3379 1936
Size: 1443 4594
SbfI CC_TGCA'GG
Cuts at: 3389 3389
Size: 6037
ScaI AGT'ACT
Cuts at: 4285 5997 4285
Size: 1712 4325
SfcI C'TryA_G
Cuts at: 826 1045 2534 3385 4520 5201 5392 826
Size: 219 1489 851 1135 681 191 1471
Fragments arranged by size:
1489 1471 1135 851 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6037
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6037
SmaI CCC'GGG
Cuts at: 1872 2174 3364 1872
Size: 302 1190 4545
Fragments arranged by size:
4545 1190 302
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4141 5042 5304 820
Size: 197 926 2198 901 262 1553
Fragments arranged by size:
2198 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3828 493
Size: 3335 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6037
SphI G_CATG'C
Cuts at: 1132 3395 1132
Size: 2263 3774
SspI AAT'ATT
Cuts at: 5 52 3961 5
Size: 47 3909 2081
Fragments arranged by size:
3909 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6037
StyI C'CwwG_G
Cuts at: 513 1065 2004 2724 3476 513
Size: 552 939 720 752 3074
Fragments arranged by size:
3074 939 752 720 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2548 2872 3001 3092 4064 4081 4234
Size: 1255 968 324 129 91 972 17 153
Cuts at: 4234 4419 4864 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 972 968 445 324 185 153
129 91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2130 4283 5995
Size: 274 80 33 51 1443 153 2153 1712
Cuts at: 5995 96
Size: 138
Fragments arranged by size:
2153 1712 1443 274 153 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5997 6029 5997
Size: 32 6005
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6037
XbaI T'CTAG_A
Cuts at: 3373 3373
Size: 6037
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6037
XmaI C'CCGG_G
Cuts at: 1870 2172 3362 1870
Size: 302 1190 4545
Fragments arranged by size:
4545 1190 302
XmnI GAAnn'nnTTC
Cuts at: 2957 4166 2957
Size: 1209 4828
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2469 3845 276
Size: 53 83 186 936 935 1376 2468
Fragments arranged by size:
2468 1376 936 935 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI