Restriction map for
pFN21AE4426
(Circular) (Six-base) MAPSORT of: pFN21AE4426.seq Check: 1221 from: 1 to: 6034
pFN21AE4426 6034 bp
With 182 enzymes: SgfI *
March 28, 2012 13:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2923 2923
Size: 6034
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3377 1937
Size: 1440 4594
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3142 4097 5481 5749 1632
Size: 1510 955 1384 268 1917
Fragments arranged by size:
1917 1510 1384 955 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 4933 5910 1203
Size: 3730 977 1327
Fragments arranged by size:
3730 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 2943 3128 1166
Size: 1777 185 4072
Fragments arranged by size:
4072 1777 185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6034
AlwNI CAG_nnn'CTG
Cuts at: 3104 3777 5349 3104
Size: 673 1572 3789
Fragments arranged by size:
3789 1572 673
ApaI G_GGCC'C
Cuts at: 2824 2824
Size: 6034
ApaLI G'TGCA_C
Cuts at: 2936 4091 5247 2936
Size: 1155 1156 3723
Fragments arranged by size:
3723 1156 1155
ApoI r'AATT_y
Cuts at: 1493 2467 3256 3343 3569 3721 5830 1493
Size: 974 789 87 226 152 2109 1697
Fragments arranged by size:
2109 1697 974 789 226 152 87
AseI AT'TA_AT
Cuts at: 160 1032 2915 4588 160
Size: 872 1883 1673 1606
Fragments arranged by size:
1883 1673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 3359 1870
Size: 73 1416 4545
Fragments arranged by size:
4545 1416 73
AvrII C'CTAG_G
Cuts at: 2941 2941
Size: 6034
BamHI G'GATC_C
Cuts at: 3364 3731 3364
Size: 367 5667
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 3355 4810 618
Size: 325 217 627 792 776 1455 1842
Fragments arranged by size:
1842 1455 792 776 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2824 3353 1391
Size: 1433 529 4072
Fragments arranged by size:
4072 1433 529
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 928
Size: 971 209 4854
Fragments arranged by size:
4854 971 209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4225 4259 1903
Size: 34 2288 34 3678
Fragments arranged by size:
3678 2288 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2352 2762 2790 3926 5136 2352
Size: 410 28 1136 1210 3250
Fragments arranged by size:
3250 1210 1136 410 28
BclI T'GATC_A
Cuts at: 1548 2343 2965 1548
Size: 795 622 4617
Fragments arranged by size:
4617 795 622
BfrBI ATG'CAT
Cuts at: 2251 2251
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2588 4645 136
Size: 107 122 71 631 605 916 2057 1525
Fragments arranged by size:
2057 1525 916 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6029 6029
Size: 6034
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2584 2824 2940 4095 5251 1790
Size: 794 240 116 1155 1156 2573
Fragments arranged by size:
2573 1156 1155 794 240 116
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6034
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2634 2818 3209
Size: 416 53 83 399 24 1217 184 391
Cuts at: 3209 3765 4723 442
Size: 556 958 1753
Fragments arranged by size:
1753 1217 958 556 416 399 391 184
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4694 1239
Size: 3455 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2485 4159 5024 5322 2485
Size: 1674 865 298 3197
Fragments arranged by size:
3197 1674 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4697 882
Size: 617 3198 2219
Fragments arranged by size:
3198 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2239 3825 493
Size: 711 1035 1586 2702
Fragments arranged by size:
2702 1586 1035 711
BsaBI GATnn'nnATC
Cuts at: 2363 6028 2363
Size: 3665 2369
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3841 4223 4858 275
Size: 53 83 186 936 2308 382 635 1451
Fragments arranged by size:
2308 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3139 4467 4865 5139 5286 5697 3139
Size: 1328 398 274 147 411 3476
Fragments arranged by size:
3476 1328 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5809
Fragments arranged by size:
5809 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2888 1186
Size: 1702 4332
BseYI C'CCAG_C
Cuts at: 5237 5733 5237
Size: 496 5538
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5461
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3484 4245 5273 1540
Size: 275 186 1483 761 1028 2301
Fragments arranged by size:
2301 1483 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2940 3166 4095 4180 5251 2940
Size: 226 929 85 1071 3723
Fragments arranged by size:
3723 1071 929 226 85
BsmI GAATG_Cn'
Cuts at: 2802 3545 3638 2802
Size: 743 93 5198
Fragments arranged by size:
5198 743 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2540 2715 2540
Size: 175 5859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2584 2824 2940 3166 3353 4095 4180
Size: 399 794 240 116 226 187 742 85
Cuts at: 4180 5251 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 794 742 399 240 226 187
116 85
BspEI T'CCGG_A
Cuts at: 3139 5697 3139
Size: 2558 3476
BspHI T'CATG_A
Cuts at: 2526 3923 5653 2526
Size: 1397 1730 2907
Fragments arranged by size:
2907 1730 1397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2923 3389 844
Size: 2079 466 3489
Fragments arranged by size:
3489 2079 466
BsrBI CCG'CTC
Cuts at: 1394 1823 3921 1394
Size: 429 2098 3507
Fragments arranged by size:
3507 2098 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4529 4703 65
Size: 1183 3281 174 1396
Fragments arranged by size:
3281 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4678 4865 1700
Size: 110 2868 187 2869
Fragments arranged by size:
2869 2868 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 2585 2868 4094 5106 2585
Size: 283 1226 1012 3513
Fragments arranged by size:
3513 1226 1012 283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6034
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6034
BstXI CCAn_nnnn'nTGG
Cuts at: 2892 2892
Size: 6034
BstYI r'GATC_y
Cuts at: 1972 3364 3731 4116 4133 5585 5671 5683 6029
Size: 1392 367 385 17 1452 86 12 346
Cuts at: 6029 1972
Size: 1977
Fragments arranged by size:
1977 1452 1392 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2572 513
Size: 552 939 568 3975
Fragments arranged by size:
3975 939 568 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 3550 4344 4364 3550
Size: 794 20 5220
Fragments arranged by size:
5220 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 3481 4370
Size: 54 1425 50 275 18 759 892 889
Cuts at: 4370 5999 8
Size: 1629 43
Fragments arranged by size:
1629 1425 892 889 759 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3481 1537
Size: 275 1669 4090
Fragments arranged by size:
4090 1669 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3964 1353
Size: 2611 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4621 4995 5141 387
Size: 1064 138 3032 374 146 1280
Fragments arranged by size:
3032 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3142 4097 4694 5481 5749 1239
Size: 393 1510 955 597 787 268 1524
Fragments arranged by size:
1524 1510 955 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3343 3721 3343
Size: 378 5656
FspI TGC'GCA
Cuts at: 4540 4540
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 5181 1678
Size: 3503 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2284 2316 4675 4707 4749
Size: 32 135 32 712 32 2359 32 42
Cuts at: 4749 4781 1373
Size: 32 2626
Fragments arranged by size:
2626 2359 712 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3378 3624 669
Size: 988 281 1440 246 3079
Fragments arranged by size:
3079 1440 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2644 2879 748
Size: 1896 235 3903
Fragments arranged by size:
3903 1896 235
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3200 3633 3200
Size: 433 5601
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2823 5148 5332 1305
Size: 1518 2325 184 2007
Fragments arranged by size:
2325 2007 1518 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 10
Size: 54 1425 343 759 3453
Fragments arranged by size:
3453 1425 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 2577 2688 2943 3128 3992 4351
Size: 648 1090 321 111 255 185 864 359
Cuts at: 4351 4510 518
Size: 159 2042
Fragments arranged by size:
2042 1090 864 648 359 321 255 185
159 111
MspA1I CmG'CkG
Cuts at: 1606 3275 3450 4127 5275 5520 1606
Size: 1669 175 677 1148 245 2120
Fragments arranged by size:
2120 1669 1148 677 245 175
NcoI C'CATG_G
Cuts at: 513 1065 2004 2572 513
Size: 552 939 568 3975
Fragments arranged by size:
3975 939 568 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NsiI A_TGCA'T
Cuts at: 2253 2253
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 3392 4937 1132
Size: 97 2163 1545 2229
Fragments arranged by size:
2229 2163 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6034
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6034
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2552 1613
Size: 939 5095
PsiI TTA'TAA
Cuts at: 2445 3604 2445
Size: 1159 4875
PspOMI G'GGCC_C
Cuts at: 2820 2820
Size: 6034
PstI C_TGCA'G
Cuts at: 830 3386 830
Size: 2556 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
PvuII CAG'CTG
Cuts at: 3275 3275
Size: 6034
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SfcI C'TryA_G
Cuts at: 826 1045 2119 2422 3031 3382 4517 5198 5389
Size: 219 1074 303 609 351 1135 681 191
Cuts at: 5389 826
Size: 1471
Fragments arranged by size:
1471 1135 1074 681 609 351 303 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 3361 1872
Size: 1489 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2500 4138 5039 5301 820
Size: 197 926 557 1638 901 262 1553
Fragments arranged by size:
1638 1553 926 901 557 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SspI AAT'ATT
Cuts at: 5 52 3958 5
Size: 47 3906 2081
Fragments arranged by size:
3906 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6034
StyI C'CwwG_G
Cuts at: 513 1065 2004 2572 2788 2941 3193 3473 513
Size: 552 939 568 216 153 252 280 3074
Fragments arranged by size:
3074 939 568 552 280 252 216 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4061 4078 4231 4416 4861 325
Size: 1255 2481 17 153 185 445 1498
Fragments arranged by size:
2481 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4280 5992 96
Size: 274 80 33 51 1443 2303 1712 138
Fragments arranged by size:
2303 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 5994 6026 2000
Size: 32 3962 32 2008
Fragments arranged by size:
3962 2008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6034
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2995 2995
Size: 6034
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6034
XmaI C'CCGG_G
Cuts at: 1870 3359 1870
Size: 1489 4545
XmnI GAAnn'nnTTC
Cuts at: 4163 4163
Size: 6034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI