Restriction map for
pFN21AE4421
(Circular) (Six-base) MAPSORT of: pFN21AE4421.seq Check: 8421 from: 1 to: 6016
pFN21AE4421 6016 bp
With 182 enzymes: SgfI *
March 28, 2012 14:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 278
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2675 3359 1937
Size: 738 684 4594
Fragments arranged by size:
4594 738 684
Acc65I G'GTAC_C
Cuts at: 3337 3337
Size: 6016
AclI AA'CG_TT
Cuts at: 1563 4143 4516 1563
Size: 2580 373 3063
Fragments arranged by size:
3063 2580 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2153 4079 5463 5731 1632
Size: 521 1926 1384 268 1917
Fragments arranged by size:
1926 1917 1384 521 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5819
AflIII A'CryG_T
Cuts at: 1203 4915 5892 1203
Size: 3712 977 1327
Fragments arranged by size:
3712 1327 977
AgeI A'CCGG_T
Cuts at: 4847 4847
Size: 6016
AhdI GACnn_n'nnGTC
Cuts at: 2089 4745 2089
Size: 2656 3360
AleI CACnn'nnGTG
Cuts at: 1166 2180 2411 2691 1166
Size: 1014 231 280 4491
Fragments arranged by size:
4491 1014 280 231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6016
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 2559
Size: 32 5984
AlwNI CAG_nnn'CTG
Cuts at: 2441 3759 5331 2441
Size: 1318 1572 3126
Fragments arranged by size:
3126 1572 1318
ApaLI G'TGCA_C
Cuts at: 4073 5229 4073
Size: 1156 4860
ApoI r'AATT_y
Cuts at: 1493 2786 2823 3325 3551 3703 5812 1493
Size: 1293 37 502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1293 502 226 152 37
AseI AT'TA_AT
Cuts at: 160 1032 4570 160
Size: 872 3538 1606
Fragments arranged by size:
3538 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 3341 1870
Size: 73 103 1295 4545
Fragments arranged by size:
4545 1295 103 73
BamHI G'GATC_C
Cuts at: 2666 3346 3713 2666
Size: 680 367 4969
Fragments arranged by size:
4969 680 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3337 4792 618
Size: 325 217 627 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2075 3335 1391
Size: 684 1260 4072
Fragments arranged by size:
4072 1260 684
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2174 2462 928
Size: 971 275 288 4482
Fragments arranged by size:
4482 971 288 275
BbvCI CC'TCA_GC
Cuts at: 2095 3225 2095
Size: 1130 4886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4207 4241 1903
Size: 34 2270 34 3678
Fragments arranged by size:
3678 2270 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2133 3908 5118 2133
Size: 1775 1210 3031
Fragments arranged by size:
3031 1775 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4627 136
Size: 107 122 71 631 605 2955 1525
Fragments arranged by size:
2955 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6011 6011
Size: 6016
BlpI GC'TnA_GC
Cuts at: 2730 2913 3433 2730
Size: 183 520 5313
Fragments arranged by size:
5313 520 183
Bme1580I G_kGCm'C
Cuts at: 1790 4077 5233 1790
Size: 2287 1156 2573
Fragments arranged by size:
2573 2287 1156
BmgBI CAC'GTC
Cuts at: 1318 3166 1318
Size: 1848 4168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2352 2385 2672
Size: 416 53 83 399 24 935 33 287
Cuts at: 2672 3747 4705 442
Size: 1075 958 1753
Fragments arranged by size:
1753 1075 958 935 416 399 287 83
53 33 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2951 4676 1239
Size: 1712 1725 2579
Fragments arranged by size:
2579 1725 1712
Bpu10I CC'TnA_GC
Cuts at: 2095 2835 3225 3249 2095
Size: 740 390 24 4862
Fragments arranged by size:
4862 740 390 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4141 5006 5304 4141
Size: 865 298 4853
Fragments arranged by size:
4853 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3281 4679 882
Size: 617 1782 1398 2219
Fragments arranged by size:
2219 1782 1398 617
BsaAI yAC'GTr
Cuts at: 493 1204 2079 2930 2976 3807 493
Size: 711 875 851 46 831 2702
Fragments arranged by size:
2702 875 851 831 711 46
BsaBI GATnn'nnATC
Cuts at: 6010 6010
Size: 6016
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4205 4840 275
Size: 53 83 186 936 2290 382 635 1451
Fragments arranged by size:
2290 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4449 4847 5121 5268 5679 4449
Size: 398 274 147 411 4786
Fragments arranged by size:
4786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2559 2589 1178
Size: 30 165 30 1156 30 4605
Fragments arranged by size:
4605 1156 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 3104 1186
Size: 1105 813 4098
Fragments arranged by size:
4098 1105 813
BseYI C'CCAG_C
Cuts at: 3291 5219 5715 3291
Size: 1928 496 3592
Fragments arranged by size:
3592 1928 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3282 1163
Size: 573 1546 3897
Fragments arranged by size:
3897 1546 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3466 4227 5255 1540
Size: 275 186 1465 761 1028 2301
Fragments arranged by size:
2301 1465 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2075 2200 2919 4077 4162 5233 2075
Size: 125 719 1158 85 1071 2858
Fragments arranged by size:
2858 1158 1071 719 125 85
BsmI GAATG_Cn'
Cuts at: 3250 3527 3620 3250
Size: 277 93 5646
Fragments arranged by size:
5646 277 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2200 2919 3335 4077 4162 5233
Size: 399 285 125 719 416 742 85 1071
Cuts at: 5233 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 719 416 399 285 125
85
BspEI T'CCGG_A
Cuts at: 5679 5679
Size: 6016
BspHI T'CATG_A
Cuts at: 3905 5635 3905
Size: 1730 4286
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 3371 844
Size: 1223 1304 3489
Fragments arranged by size:
3489 1304 1223
BsrBI CCG'CTC
Cuts at: 1394 1823 3903 1394
Size: 429 2080 3507
Fragments arranged by size:
3507 2080 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3085 4511 4685 65
Size: 1183 1837 1426 174 1396
Fragments arranged by size:
1837 1426 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4660 4847 1700
Size: 110 2850 187 2869
Fragments arranged by size:
2869 2850 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6016
BssSI C'ACGA_G
Cuts at: 2516 4076 5088 2516
Size: 1560 1012 3444
Fragments arranged by size:
3444 1560 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2015 2354 794
Size: 1221 339 4456
Fragments arranged by size:
4456 1221 339
BstBI TT'CG_AA
Cuts at: 4820 4820
Size: 6016
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6016
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 3040 2467
Size: 573 5443
BstYI r'GATC_y
Cuts at: 1972 2666 3346 3713 4098 4115 5567 5653 5665
Size: 694 680 367 385 17 1452 86 12
Cuts at: 5665 6011 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 694 680 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 513
Size: 552 939 177 4348
Fragments arranged by size:
4348 939 552 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5277
Fragments arranged by size:
5277 683 56
BtsI GCAGTG_nn'
Cuts at: 2442 2452 2530 3532 4326 4346 2442
Size: 10 78 1002 794 20 4112
Fragments arranged by size:
4112 1002 794 78 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5981
DraI TTT'AAA
Cuts at: 3094 3320 3094
Size: 226 5790
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5023 809
Size: 507 3707 1802
Fragments arranged by size:
3707 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2394 3463
Size: 54 1425 50 275 18 354 210 1069
Cuts at: 3463 4352 5981 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1069 889 354 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3463 1537
Size: 275 1651 4090
Fragments arranged by size:
4090 1651 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3946 1353
Size: 2593 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4603 4977 5123 387
Size: 1064 138 3014 374 146 1280
Fragments arranged by size:
3014 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2073 2073
Size: 6016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2951 4079 4676 5463 5731 1239
Size: 393 521 798 1128 597 787 268 1524
Fragments arranged by size:
1524 1128 798 787 597 521 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3212 3212
Size: 6016
EcoRI G'AATT_C
Cuts at: 3325 3703 3325
Size: 378 5638
FspI TGC'GCA
Cuts at: 4522 4522
Size: 6016
HaeII r_GCGC'y
Cuts at: 1678 2337 5163 1678
Size: 659 2826 2531
Fragments arranged by size:
2826 2531 659
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2182 2214 2740
Size: 32 135 32 503 32 75 32 526
Cuts at: 2740 2772 3050 3082 3160 3192 3223 3255 4657
Size: 32 278 32 78 32 31 32 1402
Cuts at: 4657 4689 4731 4763 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1402 526 503 278 135 78 75
42 32 32 32 32 32 32 32
32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2056 3184 3360 3606 669
Size: 988 281 118 1128 176 246 3079
Fragments arranged by size:
3079 1128 988 281 246 176 118
HindIII A'AGCT_T
Cuts at: 748 2681 748
Size: 1933 4083
HpaI GTT'AAC
Cuts at: 3184 3606 3184
Size: 422 5594
KpnI G_GTAC'C
Cuts at: 3341 3341
Size: 6016
MfeI C'AATT_G
Cuts at: 2940 3615 2940
Size: 675 5341
MluI A'CGCG_T
Cuts at: 5892 5892
Size: 6016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2483 3018 5130 5314 1305
Size: 1178 535 2112 184 2007
Fragments arranged by size:
2112 2007 1178 535 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 2396 10
Size: 54 1425 343 354 210 3630
Fragments arranged by size:
3630 1425 354 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 2411 2691 3044 3974 4333 4492
Size: 648 1014 231 280 353 930 359 159
Cuts at: 4492 518
Size: 2042
Fragments arranged by size:
2042 1014 930 648 359 353 280 231
159
MspA1I CmG'CkG
Cuts at: 1606 3136 3229 3432 4109 5257 5502 1606
Size: 1530 93 203 677 1148 245 2120
Fragments arranged by size:
2120 1530 1148 677 245 203 93
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4525
Fragments arranged by size:
4525 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6016
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6016
NotI GC'GGCC_GC
Cuts at: 3463 3463
Size: 6016
NspI r_CATG'y
Cuts at: 1132 1229 3374 4919 1132
Size: 97 2145 1545 2229
Fragments arranged by size:
2229 2145 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6016
PciI A'CATG_T
Cuts at: 4915 4915
Size: 6016
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6016
PmeI GTTT'AAAC
Cuts at: 3320 3320
Size: 6016
PmlI CAC'GTG
Cuts at: 2976 2976
Size: 6016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2559 2591 4085 4117 5598 5630 2559
Size: 32 1494 32 1481 32 2945
Fragments arranged by size:
2945 1494 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3212 3212
Size: 6016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6016
PsiI TTA'TAA
Cuts at: 3586 3586
Size: 6016
PstI C_TGCA'G
Cuts at: 830 3141 3368 830
Size: 2311 227 3478
Fragments arranged by size:
3478 2311 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6016
PvuII CAG'CTG
Cuts at: 3136 3229 3136
Size: 93 5923
SacI G_AGCT'C
Cuts at: 2075 2075
Size: 6016
SalI G'TCGA_C
Cuts at: 1936 3358 1936
Size: 1422 4594
SbfI CC_TGCA'GG
Cuts at: 3368 3368
Size: 6016
ScaI AGT'ACT
Cuts at: 4264 5976 4264
Size: 1712 4304
SfcI C'TryA_G
Cuts at: 826 1045 3137 3364 4499 5180 5371 826
Size: 219 2092 227 1135 681 191 1471
Fragments arranged by size:
2092 1471 1135 681 227 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6016
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6016
SmaI CCC'GGG
Cuts at: 1872 3343 1872
Size: 1471 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4120 5021 5283 820
Size: 197 926 2177 901 262 1553
Fragments arranged by size:
2177 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3807 493
Size: 3314 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6016
SphI G_CATG'C
Cuts at: 1132 3374 1132
Size: 2242 3774
SspI AAT'ATT
Cuts at: 5 52 3940 5
Size: 47 3888 2081
Fragments arranged by size:
3888 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6016
StyI C'CwwG_G
Cuts at: 513 1065 2004 2064 2382 3001 3256 3455 513
Size: 552 939 60 318 619 255 199 3074
Fragments arranged by size:
3074 939 619 552 318 255 199 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4043 4060 4213 4398 4843 325
Size: 1255 2463 17 153 185 445 1498
Fragments arranged by size:
2463 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2108 4262 5974
Size: 274 80 33 51 1443 131 2154 1712
Cuts at: 5974 96
Size: 138
Fragments arranged by size:
2154 1712 1443 274 138 131 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3003 3035 5976 6008 3003
Size: 32 2941 32 3011
Fragments arranged by size:
3011 2941 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2461 3235 1316
Size: 1145 774 4097
Fragments arranged by size:
4097 1145 774
XbaI T'CTAG_A
Cuts at: 3352 3352
Size: 6016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2389 2179
Size: 210 5806
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6016
XmaI C'CCGG_G
Cuts at: 1870 3341 1870
Size: 1471 4545
XmnI GAAnn'nnTTC
Cuts at: 4145 4145
Size: 6016
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 276
Size: 53 83 186 936 2290 2468
Fragments arranged by size:
2468 2290 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI