Restriction map for
pFN21AE4419
(Circular) (Six-base) MAPSORT of: pFN21AE4419.seq Check: 7829 from: 1 to: 6001
pFN21AE4419 6001 bp
With 182 enzymes: SgfI *
March 28, 2012 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2965 2965
Size: 6001
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 3210 4064 5448 5716 1632
Size: 527 1051 854 1384 268 1917
Fragments arranged by size:
1917 1384 1051 854 527 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 2982 4832 2982
Size: 1850 4151
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 2627 1166
Size: 1461 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3032 1184
Size: 1848 4153
AlwNI CAG_nnn'CTG
Cuts at: 2356 2443 2698 3188 3253 3744 5316 2356
Size: 87 255 490 65 491 1572 3041
Fragments arranged by size:
3041 1572 491 490 255 87 65
ApaI G_GGCC'C
Cuts at: 2376 2376
Size: 6001
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 2517 3331 3698 2517
Size: 814 367 4820
Fragments arranged by size:
4820 814 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2527 3003 3322 4777
Size: 325 217 627 357 383 476 319 1455
Cuts at: 4777 618
Size: 1842
Fragments arranged by size:
1842 1455 627 476 383 357 325 319
217
BanII G_rGCy'C
Cuts at: 1391 2376 2716 3320 1391
Size: 985 340 604 4072
Fragments arranged by size:
4072 985 604 340
BbeI G_GCGC'C
Cuts at: 2148 3007 2148
Size: 859 5142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5030
BbvCI CC'TCA_GC
Cuts at: 2220 2220
Size: 6001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4192 4226 1903
Size: 34 2255 34 3678
Fragments arranged by size:
3678 2255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4612 136
Size: 107 122 71 631 605 2940 1525
Fragments arranged by size:
2940 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 2376 2692 4062 5218 1790
Size: 586 316 1370 1156 2573
Fragments arranged by size:
2573 1370 1156 586 316
BmgBI CAC'GTC
Cuts at: 1318 2914 1318
Size: 1596 4405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2490 3241 3732
Size: 416 53 83 399 24 1073 751 491
Cuts at: 3732 4690 442
Size: 958 1753
Fragments arranged by size:
1753 1073 958 751 491 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4661 1239
Size: 3422 2579
Bpu10I CC'TnA_GC
Cuts at: 2220 2220
Size: 6001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 4126 4991 5289 2507
Size: 1619 865 298 3219
Fragments arranged by size:
3219 1619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 2465 4664 882
Size: 617 693 273 2199 2219
Fragments arranged by size:
2219 2199 693 617 273
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 3004 3808 4190
Size: 53 83 186 936 612 859 804 382
Cuts at: 4190 4825 275
Size: 635 1451
Fragments arranged by size:
1451 936 859 804 635 612 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2296 2982 4434 4832 5106 5253 5664 2296
Size: 686 1452 398 274 147 411 2633
Fragments arranged by size:
2633 1452 686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2234 2264 2321
Size: 30 165 30 708 30 93 30 57
Cuts at: 2321 2351 2703 2733 1178
Size: 30 352 30 4446
Fragments arranged by size:
4446 708 352 165 93 57 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 2483 1186
Size: 1156 141 4704
Fragments arranged by size:
4704 1156 141
BseYI C'CCAG_C
Cuts at: 3065 5204 5700 3065
Size: 2139 496 3366
Fragments arranged by size:
3366 2139 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2246 1163
Size: 573 414 96 4918
Fragments arranged by size:
4918 573 414 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2187 3451 4212 5240 1540
Size: 275 186 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 4062 4147 5218 4062
Size: 85 1071 4845
Fragments arranged by size:
4845 1071 85
BsmI GAATG_Cn'
Cuts at: 2292 3512 3605 2292
Size: 1220 93 4688
Fragments arranged by size:
4688 1220 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2174
Size: 6001
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2376 2692 2716 3320 4062 4147 5218
Size: 399 586 316 24 604 742 85 1071
Cuts at: 5218 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 604 586 399 316 85
24
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2965 3356 844
Size: 2121 391 3489
Fragments arranged by size:
3489 2121 391
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 3888 1394
Size: 429 293 1772 3507
Fragments arranged by size:
3507 1772 429 293
BsrDI GCAATG_nn'
Cuts at: 65 1248 2073 2277 4496 4670 65
Size: 1183 825 204 2219 174 1396
Fragments arranged by size:
2219 1396 1183 825 204 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2639 2856 2982 4645 4832 1700
Size: 110 829 217 126 1663 187 2869
Fragments arranged by size:
2869 1663 829 217 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2443 794
Size: 1649 4352
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 2779 1184
Size: 1595 4406
BstXI CCAn_nnnn'nTGG
Cuts at: 3172 3172
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 2517 3331 3698 4083 4100 5552 5638 5650
Size: 545 814 367 385 17 1452 86 12
Cuts at: 5650 5996 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 814 545 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2175 2763 2787 2949 3054 513
Size: 552 1110 588 24 162 105 3460
Fragments arranged by size:
3460 1110 588 552 162 105 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2137 487
Size: 683 56 911 4351
Fragments arranged by size:
4351 911 683 56
BtsI GCAGTG_nn'
Cuts at: 2234 2750 3189 3517 4311 4331 2234
Size: 516 439 328 794 20 3904
Fragments arranged by size:
3904 794 516 439 328 20
ClaI AT'CG_AT
Cuts at: 1554 2922 1554
Size: 1368 4633
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 2508 2854
Size: 54 1425 50 275 18 354 324 346
Cuts at: 2854 3021 3448 4337 5966 8
Size: 167 427 889 1629 43
Fragments arranged by size:
1629 1425 889 427 354 346 324 275
167 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2184 3448 1537
Size: 275 372 1264 4090
Fragments arranged by size:
4090 1264 372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3931 1353
Size: 2578 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 3210 4064 4661 5448 5716 1239
Size: 393 527 1051 854 597 787 268 1524
Fragments arranged by size:
1524 1051 854 787 597 527 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2065 2373 2695 2065
Size: 308 322 5371
Fragments arranged by size:
5371 322 308
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 2148 3007 5148 1678
Size: 470 859 2141 2531
Fragments arranged by size:
2531 2141 859 470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2703 2735 4642 4674 4716
Size: 32 135 32 1131 32 1907 32 42
Cuts at: 4716 4748 1373
Size: 32 2626
Fragments arranged by size:
2626 1907 1131 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3040 3222 3345 3591 669
Size: 988 281 1102 182 123 246 3079
Fragments arranged by size:
3079 1102 988 281 246 182 123
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2144 3003 2144
Size: 859 5142
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 10
Size: 54 1425 343 678 3501
Fragments arranged by size:
3501 1425 678 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 2297 2627 3959 4318 4477 518
Size: 648 1008 123 330 1332 359 159 2042
Fragments arranged by size:
2042 1332 1008 648 359 330 159 123
MspA1I CmG'CkG
Cuts at: 1606 2238 3417 4094 5242 5487 1606
Size: 632 1179 677 1148 245 2120
Fragments arranged by size:
2120 1179 1148 677 632 245
NaeI GCC'GGC
Cuts at: 2641 2858 2641
Size: 217 5784
NarI GG'CG_CC
Cuts at: 2145 3004 2145
Size: 859 5142
NcoI C'CATG_G
Cuts at: 513 1065 2763 2787 3054 513
Size: 552 1698 24 267 3460
Fragments arranged by size:
3460 1698 552 267 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NgoMIV G'CCGG_C
Cuts at: 2639 2856 2639
Size: 217 5784
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 3262 3359 4904 1132
Size: 97 2033 97 1545 2229
Fragments arranged by size:
2229 2033 1545 97 97
PasI CC'CwG_GG
Cuts at: 1348 2212 1348
Size: 864 5137
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3103 3253 1108
Size: 1995 150 3856
Fragments arranged by size:
3856 1995 150
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2186 2204 2483 1613
Size: 573 18 279 5131
Fragments arranged by size:
5131 573 279 18
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PspOMI G'GGCC_C
Cuts at: 2372 2372
Size: 6001
PstI C_TGCA'G
Cuts at: 830 2757 3353 830
Size: 1927 596 3478
Fragments arranged by size:
3478 1927 596
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2238 2238
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SexAI A'CCwGG_T
Cuts at: 3096 3096
Size: 6001
SfcI C'TryA_G
Cuts at: 826 1045 2753 3349 4484 5165 5356 826
Size: 219 1708 596 1135 681 191 1471
Fragments arranged by size:
1708 1471 1135 681 596 219 191
SfoI GGC'GCC
Cuts at: 2146 3005 2146
Size: 859 5142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 4105 5006 5268 820
Size: 197 926 579 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 579 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 2798 1517
Size: 1281 4720
StyI C'CwwG_G
Cuts at: 513 1065 2283 2763 2787 2851 3054 3440 513
Size: 552 1218 480 24 64 203 386 3074
Fragments arranged by size:
3074 1218 552 480 386 203 64 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2270 4028 4045 4198 4383 4828
Size: 1255 624 66 1758 17 153 185 445
Cuts at: 4828 325
Size: 1498
Fragments arranged by size:
1758 1498 1255 624 445 185 153 66
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2358 4247 5959
Size: 274 80 33 51 1443 381 1889 1712
Cuts at: 5959 96
Size: 138
Fragments arranged by size:
1889 1712 1443 381 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 5961 5993 2321
Size: 32 3608 32 2329
Fragments arranged by size:
3608 2329 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2552 1316
Size: 1236 4765
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3100 3100
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SrfI SwaI