Restriction map for
pFN21AE4418
(Circular) (Six-base) MAPSORT of: pFN21AE4418.seq Check: 2633 from: 1 to: 5998
pFN21AE4418 5998 bp
With 182 enzymes: SgfI *
March 28, 2012 14:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3276 3319 3276
Size: 43 5955
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2946 3244 4061 5445 5713 1632
Size: 1314 298 817 1384 268 1917
Fragments arranged by size:
1917 1384 1314 817 298 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 3132 4727 3132
Size: 1595 4403
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2701 2733 2701
Size: 32 5966
AlwNI CAG_nnn'CTG
Cuts at: 2155 2225 2566 3741 5313 2155
Size: 70 341 1175 1572 2840
Fragments arranged by size:
2840 1572 1175 341 70
ApaLI G'TGCA_C
Cuts at: 2136 3065 3215 4055 5211 2136
Size: 929 150 840 1156 2923
Fragments arranged by size:
2923 1156 929 840 150
ApoI r'AATT_y
Cuts at: 1493 2255 2953 3307 3533 3685 5794 1493
Size: 762 698 354 226 152 2109 1697
Fragments arranged by size:
2109 1697 762 698 354 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3151 3233 3323 1870
Size: 73 1208 82 90 4545
Fragments arranged by size:
4545 1208 90 82 73
BamHI G'GATC_C
Cuts at: 3328 3695 3328
Size: 367 5631
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3276 3319 4774 618
Size: 325 217 627 1489 43 1455 1842
Fragments arranged by size:
1842 1489 1455 627 325 217 43
BanII G_rGCy'C
Cuts at: 1391 3317 1391
Size: 1926 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 2055 1548
Size: 507 5491
BfrBI ATG'CAT
Cuts at: 2892 2892
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4609 136
Size: 107 122 71 631 605 2937 1525
Fragments arranged by size:
2937 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2140 3069 3192 3219 4059 5215 1790
Size: 350 929 123 27 840 1156 2573
Fragments arranged by size:
2573 1156 929 840 350 123 27
BmgBI CAC'GTC
Cuts at: 1318 3246 1318
Size: 1928 4070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2388 2489 3729
Size: 416 53 83 399 24 971 101 1240
Cuts at: 3729 4687 442
Size: 958 1753
Fragments arranged by size:
1753 1240 971 958 416 399 101 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2709 4658 1239
Size: 1470 1949 2579
Fragments arranged by size:
2579 1949 1470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2760 2956 4123 4988 5286 2760
Size: 196 1167 865 298 3472
Fragments arranged by size:
3472 1167 865 298 196
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2459 4661 882
Size: 617 960 2202 2219
Fragments arranged by size:
2219 2202 960 617
BsaAI yAC'GTr
Cuts at: 493 1204 2535 2836 3789 493
Size: 711 1331 301 953 2702
Fragments arranged by size:
2702 1331 953 711 301
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3058 3805 4187 4822
Size: 53 83 186 936 1525 747 382 635
Cuts at: 4822 275
Size: 1451
Fragments arranged by size:
1525 1451 936 747 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3069 3279 4431 4829 5103 5250 5661 3069
Size: 210 1152 398 274 147 411 3406
Fragments arranged by size:
3406 1152 411 398 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5773
Fragments arranged by size:
5773 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5998
BseYI C'CCAG_C
Cuts at: 3144 5201 5697 3144
Size: 2057 496 3445
Fragments arranged by size:
3445 2057 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3268 1163
Size: 573 1532 3893
Fragments arranged by size:
3893 1532 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2140 3069 3219 4059 4144 5215 2140
Size: 929 150 840 85 1071 2923
Fragments arranged by size:
2923 1071 929 840 150 85
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 3069 3192 3219 3317 4059 4144
Size: 399 350 929 123 27 98 742 85
Cuts at: 4144 5215 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 929 742 399 350 123 98
85 27
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 2454 3240 3887 5617 2454
Size: 786 647 1730 2835
Fragments arranged by size:
2835 1730 786 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3183 3353 844
Size: 2339 170 3489
Fragments arranged by size:
3489 2339 170
BsrBI CCG'CTC
Cuts at: 1394 1823 3166 3885 1394
Size: 429 1343 719 3507
Fragments arranged by size:
3507 1343 719 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4493 4667 65
Size: 1183 3245 174 1396
Fragments arranged by size:
3245 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 4087
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 794
Size: 1361 4637
BstBI TT'CG_AA
Cuts at: 2503 4802 2503
Size: 2299 3699
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2562 3328 3695 4080 4097 5549 5635 5647
Size: 590 766 367 385 17 1452 86 12
Cuts at: 5647 5993 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 766 590 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2077 3267 487
Size: 683 56 851 1190 3218
Fragments arranged by size:
3218 1190 851 683 56
BtsI GCAGTG_nn'
Cuts at: 3514 4308 4328 3514
Size: 794 20 5184
Fragments arranged by size:
5184 794 20
ClaI AT'CG_AT
Cuts at: 1554 2887 1554
Size: 1333 4665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DraIII CAC_nnn'GTG
Cuts at: 2497 2497
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2427 3445 4334
Size: 54 1425 50 275 18 597 1018 889
Cuts at: 4334 5963 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1018 889 597 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2730 3928 1353
Size: 1377 1198 3423
Fragments arranged by size:
3423 1377 1198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4585 4959 5105 387
Size: 1064 138 2996 374 146 1280
Fragments arranged by size:
2996 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2709 2946 3244 4061 4658 5445 5713
Size: 393 1077 237 298 817 597 787 268
Cuts at: 5713 1239
Size: 1524
Fragments arranged by size:
1524 1077 817 787 597 393 298 268
237
EcoNI CCTnn'n_nnAGG
Cuts at: 2238 2238
Size: 5998
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
EcoRV GAT'ATC
Cuts at: 2740 2740
Size: 5998
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2491 2523 3118 3150 4639
Size: 32 135 32 919 32 595 32 1489
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1489 919 595 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3280 3323 3280
Size: 43 5955
MfeI C'AATT_G
Cuts at: 2208 3597 2208
Size: 1389 4609
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2686 5112 5296 1305
Size: 1381 2426 184 2007
Fragments arranged by size:
2426 2007 1381 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 10
Size: 54 1425 343 597 3579
Fragments arranged by size:
3579 1425 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 2790 2919 3956 4315 4474 518
Size: 648 1357 267 129 1037 359 159 2042
Fragments arranged by size:
2042 1357 1037 648 359 267 159 129
MspA1I CmG'CkG
Cuts at: 1606 2068 3414 4091 5239 5484 1606
Size: 462 1346 677 1148 245 2120
Fragments arranged by size:
2120 1346 1148 677 462 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5446
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NsiI A_TGCA'T
Cuts at: 2894 2894
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2310 3356 4901 1132
Size: 97 1081 1046 1545 2229
Fragments arranged by size:
2229 1545 1081 1046 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5998
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PmlI CAC'GTG
Cuts at: 2535 2535
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PspXI vC'TCGA_Gb
Cuts at: 3151 3151
Size: 5998
PstI C_TGCA'G
Cuts at: 830 3350 830
Size: 2520 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2068 2068
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2350 2988 3346 4481 5162 5353 826
Size: 219 1305 638 358 1135 681 191 1471
Fragments arranged by size:
1471 1305 1135 681 638 358 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3235 3325 1872
Size: 1363 90 4545
Fragments arranged by size:
4545 1363 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2775 2971 3151 4102 5003 5265
Size: 197 926 832 196 180 951 901 262
Cuts at: 5265 820
Size: 1553
Fragments arranged by size:
1553 951 926 901 832 262 197 196
180
SnaBI TAC'GTA
Cuts at: 493 2836 3789 493
Size: 2343 953 2702
Fragments arranged by size:
2702 2343 953
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3199 3922 5
Size: 47 3147 723 2081
Fragments arranged by size:
3147 2081 723 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 3437 513
Size: 552 2372 3074
Fragments arranged by size:
3074 2372 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2508 2565 4025 4042 4195 4380 4825
Size: 1255 928 57 1460 17 153 185 445
Cuts at: 4825 325
Size: 1498
Fragments arranged by size:
1498 1460 1255 928 445 185 153 57
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 4244 5956
Size: 274 80 33 51 1443 30 2237 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
2237 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3048 3080 5958 5990 3048
Size: 32 2878 32 3056
Fragments arranged by size:
3056 2878 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2278 1316
Size: 962 5036
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 3151 1943
Size: 1208 4790
XmaI C'CCGG_G
Cuts at: 1870 3233 3323 1870
Size: 1363 90 4545
Fragments arranged by size:
4545 1363 90
XmnI GAAnn'nnTTC
Cuts at: 4127 4127
Size: 5998
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PpuMI PspOMI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI