Restriction map for
pFN21AE4417
(Circular) (Six-base) MAPSORT of: pFN21AE4417.seq Check: 7871 from: 1 to: 5995
pFN21AE4417 5995 bp
With 182 enzymes: SgfI *
March 28, 2012 13:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 2494 4058 5442 5710 1632
Size: 537 325 1564 1384 268 1917
Fragments arranged by size:
1917 1564 1384 537 325 268
AflII C'TTAA_G
Cuts at: 820 1017 2416 820
Size: 197 1399 4399
Fragments arranged by size:
4399 1399 197
AflIII A'CryG_T
Cuts at: 1203 4894 5871 1203
Size: 3691 977 1327
Fragments arranged by size:
3691 1327 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 2657 1166
Size: 1491 4504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 2097 2585 3738 5310 2097
Size: 488 1153 1572 2782
Fragments arranged by size:
2782 1572 1153 488
ApaI G_GGCC'C
Cuts at: 2977 2977
Size: 5995
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 2275 2315 3304 3530 3682 5791 1493
Size: 782 40 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 782 226 152 40
AseI AT'TA_AT
Cuts at: 160 1032 2811 4549 160
Size: 872 1779 1738 1606
Fragments arranged by size:
1779 1738 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2059 3320 1870
Size: 73 93 23 1261 4545
Fragments arranged by size:
4545 1261 93 73 23
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 3316 4771 618
Size: 325 217 627 411 1118 1455 1842
Fragments arranged by size:
1842 1455 1118 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 2977 3314 1391
Size: 1586 337 4072
Fragments arranged by size:
4072 1586 337
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 3186 1548
Size: 1638 4357
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 4606 136
Size: 107 122 71 631 605 403 2531 1525
Fragments arranged by size:
2531 1525 631 605 403 122 107 71
BglII A'GATC_T
Cuts at: 2931 5990 2931
Size: 3059 2936
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 2977 4056 5212 1790
Size: 1187 1079 1156 2573
Fragments arranged by size:
2573 1187 1156 1079
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2109 2430 3232 4655 1239
Size: 870 321 802 1423 2579
Fragments arranged by size:
2579 1423 870 802 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3074 4120 4985 5283 3074
Size: 1046 865 298 3786
Fragments arranged by size:
3786 1046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3085 4658 882
Size: 617 1586 1573 2219
Fragments arranged by size:
2219 1586 1573 617
BsaAI yAC'GTr
Cuts at: 493 1204 3786 493
Size: 711 2582 2702
Fragments arranged by size:
2702 2582 711
BsaBI GATnn'nnATC
Cuts at: 2936 5989 2936
Size: 3053 2942
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3802 4184 4819 275
Size: 53 83 186 936 2269 382 635 1451
Fragments arranged by size:
2269 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2671 4428 4826 5100 5247 5658 2671
Size: 1757 398 274 147 411 3008
Fragments arranged by size:
3008 1757 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5770
Fragments arranged by size:
5770 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5995
BseYI C'CCAG_C
Cuts at: 5198 5694 5198
Size: 496 5499
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2000 2411 1163
Size: 573 264 411 4747
Fragments arranged by size:
4747 573 411 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 3445 4206 5234 1540
Size: 275 186 25 1419 761 1028 2301
Fragments arranged by size:
2301 1419 1028 761 275 186 25
BsiHKAI G_wGCw'C
Cuts at: 4056 4141 5212 4056
Size: 85 1071 4839
Fragments arranged by size:
4839 1071 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2977 3314 4056 4141 5212 1391
Size: 399 1187 337 742 85 1071 2174
Fragments arranged by size:
2174 1187 1071 742 399 337 85
BspEI T'CCGG_A
Cuts at: 2671 5658 2671
Size: 2987 3008
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 3350 844
Size: 1457 1049 3489
Fragments arranged by size:
3489 1457 1049
BsrBI CCG'CTC
Cuts at: 1394 1823 3181 3882 1394
Size: 429 1358 701 3507
Fragments arranged by size:
3507 1358 701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4490 4664 65
Size: 1183 3242 174 1396
Fragments arranged by size:
3242 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2376 4639 4826 1700
Size: 110 566 2263 187 2869
Fragments arranged by size:
2869 2263 566 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 3241 1184
Size: 2057 3938
BstXI CCAn_nnnn'nTGG
Cuts at: 2659 2845 3256 2659
Size: 186 411 5398
Fragments arranged by size:
5398 411 186
BstYI r'GATC_y
Cuts at: 1972 2128 2931 3325 3692 4077 4094 5546 5632
Size: 156 803 394 367 385 17 1452 86
Cuts at: 5632 5644 5990 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 803 394 385 367 346 156
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 2652 513
Size: 552 939 69 579 3856
Fragments arranged by size:
3856 939 579 552 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5960
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2023 2374 3220
Size: 54 1425 50 275 18 193 351 846
Cuts at: 3220 3442 4331 5960 8
Size: 222 889 1629 43
Fragments arranged by size:
1629 1425 889 846 351 275 222 193
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2023 3442 1537
Size: 275 211 1419 4090
Fragments arranged by size:
4090 1419 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2363 3925 1353
Size: 1010 1562 3423
Fragments arranged by size:
3423 1562 1010
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4582 4956 5102 387
Size: 1064 138 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2169 2430 2494 3232 4058 4655
Size: 393 477 60 261 64 738 826 597
Cuts at: 4655 5442 5710 1239
Size: 787 268 1524
Fragments arranged by size:
1524 826 787 738 597 477 393 268
261 64 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2158 3092 2158
Size: 934 5061
EcoO109I rG'GnC_Cy
Cuts at: 2120 2973 2120
Size: 853 5142
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 2586 5142 1678
Size: 908 2556 2531
Fragments arranged by size:
2556 2531 908
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373
Size: 32 135 32 3064 32 42 32 2626
Fragments arranged by size:
3064 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3112 3594 3112
Size: 482 5513
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2979 3108 5109 5293 1305
Size: 1674 129 2001 184 2007
Fragments arranged by size:
2007 2001 1674 184 129
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4173
Fragments arranged by size:
4173 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 3062 3206 3230 3953 4312 4471
Size: 648 1491 405 144 24 723 359 159
Cuts at: 4471 518
Size: 2042
Fragments arranged by size:
2042 1491 723 648 405 359 159 144
24
MspA1I CmG'CkG
Cuts at: 1606 2075 3411 4088 5236 5481 1606
Size: 469 1336 677 1148 245 2120
Fragments arranged by size:
2120 1336 1148 677 469 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 2833 387
Size: 2446 3549
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 2527 2636 3067 3145 3353 4898 1132
Size: 97 1298 109 431 78 208 1545 2229
Fragments arranged by size:
2229 1545 1298 431 208 109 97 78
PasI CC'CwG_GG
Cuts at: 1348 2344 1348
Size: 996 4999
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2097 1108
Size: 989 5006
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2120 2120
Size: 5995
PshAI GACnn'nnGTC
Cuts at: 1613 2667 1613
Size: 1054 4941
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 2973 2973
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2335 3347 830
Size: 1505 1012 3478
Fragments arranged by size:
3478 1505 1012
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
SacII CC_GC'GG
Cuts at: 2076 2076
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SfcI C'TryA_G
Cuts at: 826 1045 2331 3343 4478 5159 5350 826
Size: 219 1286 1012 1135 681 191 1471
Fragments arranged by size:
1471 1286 1135 1012 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 2038 2061 3322 1872
Size: 166 23 1261 4545
Fragments arranged by size:
4545 1261 166 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2416 3089 4099 5000 5262 820
Size: 197 926 473 673 1010 901 262 1553
Fragments arranged by size:
1553 1010 926 901 673 473 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 2527 2636 3067 3353 1132
Size: 1395 109 431 286 3774
Fragments arranged by size:
3774 1395 431 286 109
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5995
StyI C'CwwG_G
Cuts at: 513 1065 2004 2743 3291 3434 513
Size: 552 939 739 548 143 3074
Fragments arranged by size:
3074 939 739 552 548 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2336 2518 3122 4022 4039 4192 4377
Size: 1255 756 182 604 900 17 153 185
Cuts at: 4377 4822 325
Size: 445 1498
Fragments arranged by size:
1498 1255 900 756 604 445 185 182
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4241 5953 96
Size: 274 80 33 51 1443 2264 1712 138
Fragments arranged by size:
2264 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2305 2305
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 2036 2059 3320 1870
Size: 166 23 1261 4545
Fragments arranged by size:
4545 1261 166 23
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI