Restriction map for
pFN21AE4415
(Circular) (Six-base) MAPSORT of: pFN21AE4415.seq Check: 3118 from: 1 to: 5992
pFN21AE4415 5992 bp
With 182 enzymes: SgfI *
March 28, 2012 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3139 3225 4055 5439 5707 1632
Size: 1507 86 830 1384 268 1917
Fragments arranged by size:
1917 1507 1384 830 268 86
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 2306 2350 2662 3735 5307 2306
Size: 44 312 1073 1572 2991
Fragments arranged by size:
2991 1572 1073 312 44
ApaI G_GGCC'C
Cuts at: 2462 2462
Size: 5992
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
BamHI G'GATC_C
Cuts at: 2994 3322 3689 2994
Size: 328 367 5297
Fragments arranged by size:
5297 367 328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3092 3313 4768 618
Size: 325 217 627 1305 221 1455 1842
Fragments arranged by size:
1842 1455 1305 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2297 2462 2633 3311 1391
Size: 906 165 171 678 4072
Fragments arranged by size:
4072 906 678 171 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5021
BbvCI CC'TCA_GC
Cuts at: 3288 3288
Size: 5992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2829 3227 4603
Size: 107 122 71 631 605 1157 398 1376
Cuts at: 4603 136
Size: 1525
Fragments arranged by size:
1525 1376 1157 631 605 398 122 107
71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 2462 2527 2809 3076 4053 5209 1790
Size: 672 65 282 267 977 1156 2573
Fragments arranged by size:
2573 1156 977 672 282 267 65
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2749 3723 4681
Size: 416 53 83 399 24 1332 974 958
Cuts at: 4681 442
Size: 1753
Fragments arranged by size:
1753 1332 974 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3093 3093
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2311 2469 4652 1239
Size: 1072 158 2183 2579
Fragments arranged by size:
2579 2183 1072 158
Bpu10I CC'TnA_GC
Cuts at: 3288 3288
Size: 5992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4117 4982 5280 4117
Size: 865 298 4829
Fragments arranged by size:
4829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4655 882
Size: 617 3156 2219
Fragments arranged by size:
3156 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3799 4181 4816 275
Size: 53 83 186 936 2266 382 635 1451
Fragments arranged by size:
2266 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5767
Fragments arranged by size:
5767 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2657 3016 1186
Size: 1471 359 4162
Fragments arranged by size:
4162 1471 359
BseYI C'CCAG_C
Cuts at: 2350 2455 3143 5195 5691 2350
Size: 105 688 2052 496 2651
Fragments arranged by size:
2651 2052 688 496 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2233 2297 2651 2691 3188 4053 4138 5209 2233
Size: 64 354 40 497 865 85 1071 3016
Fragments arranged by size:
3016 1071 865 497 354 85 64 40
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2297 2462 2527 2633 2651 2691
Size: 399 443 64 165 65 106 18 40
Cuts at: 2691 2809 3076 3188 3311 4053 4138 5209 1391
Size: 118 267 112 123 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 399 267 165 123
118 112 106 85 65 64 40 18
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 2922 3881 5611 2922
Size: 959 1730 3303
Fragments arranged by size:
3303 1730 959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2584 2735 3347 844
Size: 1740 151 612 3489
Fragments arranged by size:
3489 1740 612 151
BsrBI CCG'CTC
Cuts at: 1394 1823 3879 1394
Size: 429 2056 3507
Fragments arranged by size:
3507 2056 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3039 3162 4487 4661 65
Size: 1183 1791 123 1325 174 1396
Fragments arranged by size:
1791 1396 1325 1183 174 123
BsrFI r'CCGG_y
Cuts at: 1700 1810 4636 4823 1700
Size: 110 2826 187 2869
Fragments arranged by size:
2869 2826 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 4052 5064 4052
Size: 1012 4980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2183 2662 794
Size: 1389 479 4124
Fragments arranged by size:
4124 1389 479
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 2699 1184
Size: 1515 4477
BstXI CCAn_nnnn'nTGG
Cuts at: 2021 2021
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 2127 2994 3322 3689 4074 4091 5543 5629
Size: 155 867 328 367 385 17 1452 86
Cuts at: 5629 5641 5987 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 867 385 367 346 328 155
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2865 2905 513
Size: 552 939 861 40 3600
Fragments arranged by size:
3600 939 861 552 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2821 487
Size: 683 56 1595 3658
Fragments arranged by size:
3658 1595 683 56
BtsI GCAGTG_nn'
Cuts at: 3508 4302 4322 3508
Size: 794 20 5178
Fragments arranged by size:
5178 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3070 3105 529
Size: 35 2506 35 3416
Fragments arranged by size:
3416 2506 35 35
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DraIII CAC_nnn'GTG
Cuts at: 2415 2531 2415
Size: 116 5876
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3120 3219 3439
Size: 54 1425 50 275 18 1290 99 220
Cuts at: 3439 4328 5957 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1290 889 275 220 99 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2246 2251 2960 3922 1353
Size: 765 128 5 709 962 3423
Fragments arranged by size:
3423 962 765 709 128 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2295 2295
Size: 5992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2469 3139 3225 4055 4652 5439
Size: 393 679 158 670 86 830 597 787
Cuts at: 5439 5707 1239
Size: 268 1524
Fragments arranged by size:
1524 830 787 679 670 597 393 268
158 86
EcoNI CCTnn'n_nnAGG
Cuts at: 2342 2759 2342
Size: 417 5575
EcoO109I rG'GnC_Cy
Cuts at: 2374 2459 2821 2374
Size: 85 362 5545
Fragments arranged by size:
5545 362 85
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 5992
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 5139 1678
Size: 3461 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2215 2247 4633 4665 4707
Size: 32 135 32 643 32 2386 32 42
Cuts at: 4707 4739 1373
Size: 32 2626
Fragments arranged by size:
2626 2386 643 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2275 2430 3336 3582 669
Size: 988 281 337 155 906 246 3079
Fragments arranged by size:
3079 988 906 337 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2566 2910 3161 5106 5290 1305
Size: 1261 344 251 1945 184 2007
Fragments arranged by size:
2007 1945 1261 344 251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3122 10
Size: 54 1425 343 1290 2880
Fragments arranged by size:
2880 1425 1290 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2939 3263 3950 4309 4468 518
Size: 648 1773 324 687 359 159 2042
Fragments arranged by size:
2042 1773 687 648 359 324 159
MspA1I CmG'CkG
Cuts at: 1606 2496 2843 3408 4085 5233 5478 1606
Size: 890 347 565 677 1148 245 2120
Fragments arranged by size:
2120 1148 890 677 565 347 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2865 2905 513
Size: 552 939 861 40 3600
Fragments arranged by size:
3600 939 861 552 40
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 3350 4895 1132
Size: 97 2121 1545 2229
Fragments arranged by size:
2229 2121 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2014 2344 1348
Size: 666 330 4996
Fragments arranged by size:
4996 666 330
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 2991 1108
Size: 1242 641 4109
Fragments arranged by size:
4109 1242 641
PfoI T'CCnGG_A
Cuts at: 2760 2760
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 2374
Size: 5992
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PspOMI G'GGCC_C
Cuts at: 2458 2458
Size: 5992
PstI C_TGCA'G
Cuts at: 830 2658 3344 830
Size: 1828 686 3478
Fragments arranged by size:
3478 1828 686
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
PvuII CAG'CTG
Cuts at: 2496 2843 2496
Size: 347 5645
SacI G_AGCT'C
Cuts at: 2297 2297
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SapI GCTCTTCn'nnn_
Cuts at: 2246 2246
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 2658 3344 2658
Size: 686 5306
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SfcI C'TryA_G
Cuts at: 826 1045 2065 2491 2654 3175 3340 4475 5156
Size: 219 1020 426 163 521 165 1135 681
Cuts at: 5156 5347 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1020 681 521 426 219 191
165 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4096 4997 5259 820
Size: 197 926 2153 901 262 1553
Fragments arranged by size:
2153 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 2004 2865 2905 3431 513
Size: 552 939 861 40 526 3074
Fragments arranged by size:
3074 939 861 552 526 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2674 3187 4019 4036 4189 4374 4819
Size: 1255 1094 513 832 17 153 185 445
Cuts at: 4819 325
Size: 1498
Fragments arranged by size:
1498 1255 1094 832 513 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2732 4238 5950
Size: 274 80 33 51 1443 755 1506 1712
Cuts at: 5950 96
Size: 138
Fragments arranged by size:
1712 1506 1443 755 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5952 5984 5952
Size: 32 5960
Tth111I GACn'n_nGTC
Cuts at: 1316 2941 1316
Size: 1625 4367
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2440 2440
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI