Restriction map for
pFN21AE4414
(Circular) (Six-base) MAPSORT of: pFN21AE4414.seq Check: 2443 from: 1 to: 5986
pFN21AE4414 5986 bp
With 182 enzymes: SgfI *
February 2, 2012 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2924 3329 1937
Size: 987 405 4594
Fragments arranged by size:
4594 987 405
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4049 5433 5701 1632
Size: 2417 1384 268 1917
Fragments arranged by size:
2417 1917 1384 268
AfeI AGC'GCT
Cuts at: 2054 2454 2054
Size: 400 5586
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 3051 4885 5862 1203
Size: 1848 1834 977 1327
Fragments arranged by size:
1848 1834 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2858 1184
Size: 1674 4312
AlwNI CAG_nnn'CTG
Cuts at: 2108 3157 3729 5301 2108
Size: 1049 572 1572 2793
Fragments arranged by size:
2793 1572 1049 572
ApaI G_GGCC'C
Cuts at: 2378 3178 2378
Size: 800 5186
ApaLI G'TGCA_C
Cuts at: 2809 2897 4043 5199 2809
Size: 88 1146 1156 3596
Fragments arranged by size:
3596 1156 1146 88
ApoI r'AATT_y
Cuts at: 1493 3295 3521 3673 5782 1493
Size: 1802 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2952 3311 1870
Size: 73 1009 359 4545
Fragments arranged by size:
4545 1009 359 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2362 2401 2491 2709 2773
Size: 325 217 627 575 39 90 218 64
Cuts at: 2773 2780 3307 4762 618
Size: 7 527 1455 1842
Fragments arranged by size:
1842 1455 627 575 527 325 218 217
90 64 39 7
BanII G_rGCy'C
Cuts at: 1391 2378 3178 3305 1391
Size: 987 800 127 4072
Fragments arranged by size:
4072 987 800 127
BbeI G_GCGC'C
Cuts at: 2366 2405 2366
Size: 39 5947
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2087 2858 4597
Size: 107 122 71 631 605 415 771 1739
Cuts at: 4597 136
Size: 1525
Fragments arranged by size:
1739 1525 771 631 605 415 122 107
71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3233 3403 3233
Size: 170 5816
Bme1580I G_kGCm'C
Cuts at: 1790 2092 2378 2496 2712 2783 2813 2901 3178
Size: 302 286 118 216 71 30 88 277
Cuts at: 3178 4047 5203 1790
Size: 869 1156 2573
Fragments arranged by size:
2573 1156 869 302 286 277 216 118
88 71 30
BmgBI CAC'GTC
Cuts at: 1318 2770 1318
Size: 1452 4534
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2715 3717 4675
Size: 416 53 83 399 24 1298 1002 958
Cuts at: 4675 442
Size: 1753
Fragments arranged by size:
1753 1298 1002 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2354 2835 2891 3200 4646 1239
Size: 842 273 481 56 309 1446 2579
Fragments arranged by size:
2579 1446 842 481 309 273 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 4111 4976 5274 2059
Size: 2052 865 298 2771
Fragments arranged by size:
2771 2052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2896 3777 493
Size: 711 1692 881 2702
Fragments arranged by size:
2702 1692 881 711
BsaBI GATnn'nnATC
Cuts at: 2738 2915 5980 2738
Size: 177 3065 2744
Fragments arranged by size:
3065 2744 177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2044 2363 2402 2520
Size: 53 83 186 936 511 319 39 118
Cuts at: 2520 3793 4175 4810 275
Size: 1273 382 635 1451
Fragments arranged by size:
1451 1273 936 635 511 382 319 186
118 83 53 39
BsaWI w'CCGG_w
Cuts at: 3078 4419 4817 5091 5238 5649 3078
Size: 1341 398 274 147 411 3415
Fragments arranged by size:
3415 1341 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 1186
Size: 1024 4962
BseYI C'CCAG_C
Cuts at: 2085 5189 5685 2085
Size: 3104 496 2386
Fragments arranged by size:
3104 2386 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 2408 2818 1163
Size: 573 603 69 410 4331
Fragments arranged by size:
4331 603 573 410 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2508 2813 2901 4047 4132 5203 2162
Size: 346 305 88 1146 85 1071 2945
Fragments arranged by size:
2945 1146 1071 346 305 88 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
BsmBI CGTCTCn'nnnn_
Cuts at: 2035 2486 2035
Size: 451 5535
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2162 2378 2496 2508 2712 2783
Size: 399 302 70 216 118 12 204 71
Cuts at: 2783 2813 2901 3178 3305 4047 4132 5203 1391
Size: 30 88 277 127 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 302 277 216 204
127 118 88 85 71 70 30 12
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 2265 2907 3875 5605 2265
Size: 642 968 1730 2646
Fragments arranged by size:
2646 1730 968 642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3153 3341 844
Size: 2309 188 3489
Fragments arranged by size:
3489 2309 188
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 4630 4817 1700
Size: 110 271 2549 187 2869
Fragments arranged by size:
2869 2549 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 2161 4046 5058 2161
Size: 1885 1012 3089
Fragments arranged by size:
3089 1885 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 794
Size: 1535 4451
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2014 3316 3683 4068 4085 5537 5623 5635
Size: 42 1302 367 385 17 1452 86 12
Cuts at: 5635 5981 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1302 385 367 346 86 42
17 12
BstZ17I GTA'TAC
Cuts at: 2925 2925
Size: 5986
BtgI C'CryG_G
Cuts at: 513 1065 2004 2676 2853 3105 513
Size: 552 939 672 177 252 3394
Fragments arranged by size:
3394 939 672 552 252 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3237 3502 4296 4316 3237
Size: 265 794 20 4907
Fragments arranged by size:
4907 794 265 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DraIII CAC_nnn'GTG
Cuts at: 2787 2787
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2426 2466
Size: 54 1425 50 275 18 249 347 40
Cuts at: 2466 2589 2617 2679 2850 3039 3433 4322 5951
Size: 123 28 62 171 189 394 889 1629
Cuts at: 5951 8
Size: 43
Fragments arranged by size:
1629 1425 889 394 347 275 249 189
171 123 62 54 50 43 40 28
18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3916 1353
Size: 2563 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2564 3047 4573 4947 5093 387
Size: 1064 138 975 483 1526 374 146 1280
Fragments arranged by size:
1526 1280 1064 975 483 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2354 2835 2891 3200 4049 4646
Size: 393 449 273 481 56 309 849 597
Cuts at: 4646 5433 5701 1239
Size: 787 268 1524
Fragments arranged by size:
1524 849 787 597 481 449 393 309
273 268 56
EcoO109I rG'GnC_Cy
Cuts at: 2034 2374 3175 2034
Size: 340 801 4845
Fragments arranged by size:
4845 801 340
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
EcoRV GAT'ATC
Cuts at: 2917 2917
Size: 5986
FspI TGC'GCA
Cuts at: 2513 4492 2513
Size: 1979 4007
HaeII r_GCGC'y
Cuts at: 1678 2056 2171 2303 2366 2405 2456 2556 5133
Size: 378 115 132 63 39 51 100 2577
Cuts at: 5133 1678
Size: 2531
Fragments arranged by size:
2577 2531 378 132 115 100 63 51
39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4627 4659 4701 4733 1373
Size: 32 135 32 3055 32 42 32 2626
Fragments arranged by size:
3055 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2594 748
Size: 1846 4140
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KasI G'GCGC_C
Cuts at: 2362 2401 2362
Size: 39 5947
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 3051 5862 3051
Size: 2811 3175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2468 2591 10
Size: 54 1425 343 636 123 3405
Fragments arranged by size:
3405 1425 636 343 123 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3944 4303 4462 518
Size: 648 2778 359 159 2042
Fragments arranged by size:
2778 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2260 2407 2517 2948 3402 4079 5227 5472
Size: 654 147 110 431 454 677 1148 245
Cuts at: 5472 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 654 454 431 245 147
110
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 5986
NarI GG'CG_CC
Cuts at: 2363 2402 2363
Size: 39 5947
NcoI C'CATG_G
Cuts at: 513 1065 2004 3105 513
Size: 552 939 1101 3394
Fragments arranged by size:
3394 1101 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2135 3344 4889 1132
Size: 97 906 1209 1545 2229
Fragments arranged by size:
2229 1545 1209 906 97
PasI CC'CwG_GG
Cuts at: 1348 3244 1348
Size: 1896 4090
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2664 1108
Size: 1556 4430
PfoI T'CCnGG_A
Cuts at: 3087 3087
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PmlI CAC'GTG
Cuts at: 2896 2896
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspOMI G'GGCC_C
Cuts at: 2374 3174 2374
Size: 800 5186
PstI C_TGCA'G
Cuts at: 830 3150 3338 830
Size: 2320 188 3478
Fragments arranged by size:
3478 2320 188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SexAI A'CCwGG_T
Cuts at: 2487 2487
Size: 5986
SfcI C'TryA_G
Cuts at: 826 1045 3146 3334 4469 5150 5341 826
Size: 219 2101 188 1135 681 191 1471
Fragments arranged by size:
2101 1471 1135 681 219 191 188
SfiI GGCCn_nnn'nGGCC
Cuts at: 2858 2858
Size: 5986
SfoI GGC'GCC
Cuts at: 2364 2403 2364
Size: 39 5947
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 4090 4991 5253 820
Size: 197 926 95 2052 901 262 1553
Fragments arranged by size:
2052 1553 926 901 262 197 95
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 2135 3344 1132
Size: 1003 1209 3774
Fragments arranged by size:
3774 1209 1003
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2004 2443 3105 3282 3425 513
Size: 552 939 439 662 177 143 3074
Fragments arranged by size:
3074 939 662 552 439 177 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2285 2656 2813 4013 4030 4183 4368
Size: 1255 705 371 157 1200 17 153 185
Cuts at: 4368 4813 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1200 705 445 371 185 157
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2109 2141 5946 5978 2109
Size: 32 3805 32 2117
Fragments arranged by size:
3805 2117 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI Bsu36I EcoICRI EcoNI FalI FseI
FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SrfI SwaI XcmI