Restriction map for
pFN21AE4413
(Circular) (Six-base) MAPSORT of: pFN21AE4413.seq Check: 3700 from: 1 to: 5983
pFN21AE4413 5983 bp
With 182 enzymes: SgfI *
March 28, 2012 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 2538 3304 2538
Size: 766 5217
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4046 5430 5698 1632
Size: 2414 1384 268 1917
Fragments arranged by size:
2414 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 2105 4882 5859 1203
Size: 902 2777 977 1327
Fragments arranged by size:
2777 1327 977 902
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 2106 2972 1166
Size: 940 866 4177
Fragments arranged by size:
4177 940 866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2492 1184
Size: 1308 4675
AlwNI CAG_nnn'CTG
Cuts at: 3154 3726 5298 3154
Size: 572 1572 3839
Fragments arranged by size:
3839 1572 572
ApaI G_GGCC'C
Cuts at: 2407 2407
Size: 5983
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2280 2346 3174 3308 1870
Size: 73 337 66 828 134 4545
Fragments arranged by size:
4545 828 337 134 73 66
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2538 2576 2629 3197 3304
Size: 325 217 627 751 38 53 568 107
Cuts at: 3304 4759 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 751 627 568 325 217 107
53 38
BanII G_rGCy'C
Cuts at: 1391 2407 3302 1391
Size: 1016 895 4072
Fragments arranged by size:
4072 1016 895
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2450 3045 928
Size: 971 551 595 3866
Fragments arranged by size:
3866 971 595 551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 2088 1548
Size: 540 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2714 4594 136
Size: 107 122 71 631 605 1042 1880 1525
Fragments arranged by size:
1880 1525 1042 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2407 2634 3200 4044 5200 1790
Size: 617 227 566 844 1156 2573
Fragments arranged by size:
2573 1156 844 617 566 227
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 3065 3086 3098 4643 1239
Size: 974 852 21 12 1545 2579
Fragments arranged by size:
2579 1545 974 852 21 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2005 4646 882
Size: 617 506 2641 2219
Fragments arranged by size:
2641 2219 617 506
BsaAI yAC'GTr
Cuts at: 493 1204 2108 3774 493
Size: 711 904 1666 2702
Fragments arranged by size:
2702 1666 904 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 4172 4807 275
Size: 53 83 186 936 2257 382 635 1451
Fragments arranged by size:
2257 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3030 4416 4814 5088 5235 5646 3030
Size: 1386 398 274 147 411 3367
Fragments arranged by size:
3367 1386 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 1178
Size: 30 165 30 858 30 4870
Fragments arranged by size:
4870 858 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 2938 1186
Size: 1468 284 4231
Fragments arranged by size:
4231 1468 284
BseYI C'CCAG_C
Cuts at: 5186 5682 5186
Size: 496 5487
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 2674 2703 1163
Size: 573 609 329 29 4443
Fragments arranged by size:
4443 609 573 329 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4044 4129 5200 4044
Size: 85 1071 4827
Fragments arranged by size:
4827 1071 85
BsmI GAATG_Cn'
Cuts at: 2803 3494 3587 2803
Size: 691 93 5199
Fragments arranged by size:
5199 691 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2407 2634 3200 3302 4044 4129 5200
Size: 399 617 227 566 102 742 85 1071
Cuts at: 5200 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 617 566 399 227 102
85
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 2439 2451 2763 3872 5602 2439
Size: 12 312 1109 1730 2820
Fragments arranged by size:
2820 1730 1109 312 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2505 4478 4652 65
Size: 1183 1257 1973 174 1396
Fragments arranged by size:
1973 1396 1257 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 3898
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 2480 4043 5055 2480
Size: 1563 1012 3408
Fragments arranged by size:
3408 1563 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2971 2971
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 3313 3680 4065 4082 5534 5620 5632 5978
Size: 1341 367 385 17 1452 86 12 346
Cuts at: 5978 1972
Size: 1977
Fragments arranged by size:
1977 1452 1341 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2709 513
Size: 552 939 705 3787
Fragments arranged by size:
3787 939 705 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2077 3103 487
Size: 683 56 851 1026 3367
Fragments arranged by size:
3367 1026 851 683 56
BtsI GCAGTG_nn'
Cuts at: 2691 3198 3499 4293 4313 2691
Size: 507 301 794 20 4361
Fragments arranged by size:
4361 794 507 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2706 3430 4319
Size: 54 1425 50 275 18 876 724 889
Cuts at: 4319 5948 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 876 724 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2378 3913 1353
Size: 773 252 1535 3423
Fragments arranged by size:
3423 1535 773 252
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2167 2300 4570 4944 5090 387
Size: 1064 138 578 133 2270 374 146 1280
Fragments arranged by size:
2270 1280 1064 578 374 146 138 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 3065 3086 3098 4046 4643 5430
Size: 393 581 852 21 12 948 597 787
Cuts at: 5430 5698 1239
Size: 268 1524
Fragments arranged by size:
1524 948 852 787 597 581 393 268
21 12
EcoO109I rG'GnC_Cy
Cuts at: 2403 2404 2403
Size: 1 5982
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 5983
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 5130 1678
Size: 3452 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 2758 2790 4624
Size: 32 135 32 800 32 354 32 1834
Cuts at: 4624 4656 4698 4730 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1834 800 354 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 2542 3308 2542
Size: 766 5217
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 2972 3941 4300 4459 518
Size: 648 940 866 969 359 159 2042
Fragments arranged by size:
2042 969 940 866 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3112 3399 4076 5224 5469 1606
Size: 1506 287 677 1148 245 2120
Fragments arranged by size:
2120 1506 1148 677 287 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4492
Fragments arranged by size:
4492 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 3341 4886 1132
Size: 97 2112 1545 2229
Fragments arranged by size:
2229 2112 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PmlI CAC'GTG
Cuts at: 2108 2108
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2254 4052 4084 5565 5597 2222
Size: 32 1798 32 1481 32 2608
Fragments arranged by size:
2608 1798 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PspOMI G'GGCC_C
Cuts at: 2403 2403
Size: 5983
PstI C_TGCA'G
Cuts at: 830 2806 3335 830
Size: 1976 529 3478
Fragments arranged by size:
3478 1976 529
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SapI GCTCTTCn'nnn_
Cuts at: 2126 2378 2126
Size: 252 5731
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2802 3331 4466 5147 5338 826
Size: 219 1757 529 1135 681 191 1471
Fragments arranged by size:
1757 1471 1135 681 529 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2714 2714
Size: 5983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 2282 3176 3310 1872
Size: 410 894 134 4545
Fragments arranged by size:
4545 894 410 134
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4087 4988 5250 820
Size: 197 926 2144 901 262 1553
Fragments arranged by size:
2144 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 2561 152
Size: 2409 3574
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 2004 3422 513
Size: 552 939 1418 3074
Fragments arranged by size:
3074 1418 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2564 2669 3218 4010 4027 4180 4365
Size: 1255 984 105 549 792 17 153 185
Cuts at: 4365 4810 325
Size: 445 1498
Fragments arranged by size:
1498 1255 984 792 549 445 185 153
105 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2232 4229
Size: 274 80 33 51 1443 204 51 1997
Cuts at: 4229 5941 96
Size: 1712 138
Fragments arranged by size:
1997 1712 1443 274 204 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2509 2541 2938 2970 5943 5975 2097
Size: 32 380 32 397 32 2973 32 2105
Fragments arranged by size:
2973 2105 397 380 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2077 2222 2642 2971 2035
Size: 42 145 420 329 5047
Fragments arranged by size:
5047 420 329 145 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 2280 3174 3308 1870
Size: 410 894 134 4545
Fragments arranged by size:
4545 894 410 134
XmnI GAAnn'nnTTC
Cuts at: 4112 4112
Size: 5983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfoI SrfI
SwaI