Restriction map for
pFN21AE4411
(Circular) (Six-base) MAPSORT of: pFN21AE4411.seq Check: 8589 from: 1 to: 5977
pFN21AE4411 5977 bp
With 182 enzymes: SgfI *
March 28, 2012 13:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2568 3045 3320 1937
Size: 631 477 275 4594
Fragments arranged by size:
4594 631 477 275
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2342 2643 4040 5424 5692 1632
Size: 710 301 1397 1384 268 1917
Fragments arranged by size:
1917 1397 1384 710 301 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 2846 4876 5853 1203
Size: 1643 2030 977 1327
Fragments arranged by size:
2030 1643 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AlwNI CAG_nnn'CTG
Cuts at: 3720 5292 3720
Size: 1572 4405
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2853 2882 3286 3512 3664 5773 1493
Size: 1360 29 404 226 152 2109 1697
Fragments arranged by size:
2109 1697 1360 404 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2832 3302 1870
Size: 73 889 470 4545
Fragments arranged by size:
4545 889 470 73
AvrII C'CTAG_G
Cuts at: 2578 2578
Size: 5977
BamHI G'GATC_C
Cuts at: 3205 3307 3674 3205
Size: 102 367 5508
Fragments arranged by size:
5508 367 102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2779 3298 4753 618
Size: 325 217 627 992 519 1455 1842
Fragments arranged by size:
1842 1455 992 627 519 325 217
BanII G_rGCy'C
Cuts at: 1391 2056 3296 1391
Size: 665 1240 4072
Fragments arranged by size:
4072 1240 665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 2097 928
Size: 971 147 51 4808
Fragments arranged by size:
4808 971 147 51
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2583 2788 4588
Size: 107 122 71 631 605 911 205 1800
Cuts at: 4588 136
Size: 1525
Fragments arranged by size:
1800 1525 911 631 605 205 122 107
71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 2688 3394 2688
Size: 706 5271
Bme1580I G_kGCm'C
Cuts at: 1790 2798 4038 5194 1790
Size: 1008 1240 1156 2573
Fragments arranged by size:
2573 1240 1156 1008
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3005 3708 4666
Size: 416 53 83 399 24 1588 703 958
Cuts at: 4666 442
Size: 1753
Fragments arranged by size:
1753 1588 958 703 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2742 2792 3022 4637 1239
Size: 903 600 50 230 1615 2579
Fragments arranged by size:
2579 1615 903 600 230 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4102 4967 5265 4102
Size: 865 298 4814
Fragments arranged by size:
4814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4640 882
Size: 617 3141 2219
Fragments arranged by size:
3141 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3235 3768 493
Size: 711 2031 533 2702
Fragments arranged by size:
2702 2031 711 533
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3784 4166 4801 275
Size: 53 83 186 936 2251 382 635 1451
Fragments arranged by size:
2251 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2511 4410 4808 5082 5229 5640 2511
Size: 1899 398 274 147 411 2848
Fragments arranged by size:
2848 1899 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2790 2820 1178
Size: 30 165 30 1387 30 4335
Fragments arranged by size:
4335 1387 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 3173 1186
Size: 1330 657 3990
Fragments arranged by size:
3990 1330 657
BseYI C'CCAG_C
Cuts at: 2061 2065 2921 5180 5676 2061
Size: 4 856 2259 496 2362
Fragments arranged by size:
2362 2259 856 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2728 4038 4123 5194 2728
Size: 1310 85 1071 3511
Fragments arranged by size:
3511 1310 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 2728 2798 3296 4038 4123 5194
Size: 399 266 672 70 498 742 85 1071
Cuts at: 5194 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 672 498 399 266 85
70
BspEI T'CCGG_A
Cuts at: 2511 5640 2511
Size: 3129 2848
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 3864 1394
Size: 429 295 1746 3507
Fragments arranged by size:
3507 1746 429 295
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 2548 96
Size: 2452 3525
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 3125 3125
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 3205 3307 3674 4059 4076 5528 5614 5626
Size: 1233 102 367 385 17 1452 86 12
Cuts at: 5626 5972 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1233 385 367 346 102 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2014 3148 513
Size: 552 949 1134 3342
Fragments arranged by size:
3342 1134 949 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 2826 3058 3261 3493 4287 4307 2826
Size: 232 203 232 794 20 4496
Fragments arranged by size:
4496 794 232 232 203 20
ClaI AT'CG_AT
Cuts at: 1554 2757 1554
Size: 1203 4774
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3136 3281 3136
Size: 145 5832
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 3424 4313
Size: 54 1425 50 275 18 582 1012 889
Cuts at: 4313 5942 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1012 889 582 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 3907 1353
Size: 957 1597 3423
Fragments arranged by size:
3423 1597 957
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2342 2643 2742 2792 3022 4040
Size: 393 510 200 301 99 50 230 1018
Cuts at: 4040 4637 5424 5692 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1018 787 597 510 393 301 268
230 200 99 50
EcoNI CCTnn'n_nnAGG
Cuts at: 2577 2969 2577
Size: 392 5585
EcoO109I rG'GnC_Cy
Cuts at: 2709 2983 2709
Size: 274 5703
EcoRI G'AATT_C
Cuts at: 2853 3286 3664 2853
Size: 433 378 5166
Fragments arranged by size:
5166 433 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2632 2975 2632
Size: 343 5634
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 5124 1678
Size: 3446 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 2995 3027 4618
Size: 32 135 32 659 32 732 32 1591
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1591 732 659 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3046 3321 3567 669
Size: 988 281 1108 275 246 3079
Fragments arranged by size:
3079 1108 988 281 275 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 2471 3576 2471
Size: 1105 4872
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2414 10
Size: 54 1425 343 582 3573
Fragments arranged by size:
3573 1425 582 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2019 3935 4294 4453 518
Size: 648 853 1916 359 159 2042
Fragments arranged by size:
2042 1916 853 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 3393 4070 5218 5463 1606
Size: 459 1328 677 1148 245 2120
Fragments arranged by size:
2120 1328 1148 677 459 245
NcoI C'CATG_G
Cuts at: 513 1065 3148 513
Size: 552 2083 3342
Fragments arranged by size:
3342 2083 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2180 2850 3335 4880 1132
Size: 97 951 670 485 1545 2229
Fragments arranged by size:
2229 1545 951 670 485 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 2846 4876 2846
Size: 2030 3947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 1108
Size: 1668 4309
PfoI T'CCnGG_A
Cuts at: 3117 3117
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PmlI CAC'GTG
Cuts at: 3235 3235
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2949 2981 4046 4078 5559 5591 2949
Size: 32 1065 32 1481 32 3335
Fragments arranged by size:
3335 1481 1065 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2709 2709
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PstI C_TGCA'G
Cuts at: 830 3230 3329 830
Size: 2400 99 3478
Fragments arranged by size:
3478 2400 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2065 2065
Size: 5977
SalI G'TCGA_C
Cuts at: 1936 3044 3319 1936
Size: 1108 275 4594
Fragments arranged by size:
4594 1108 275
SapI GCTCTTCn'nnn_
Cuts at: 2310 2310
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 3220 3226 3325 4460 5141 5332 826
Size: 219 2175 6 99 1135 681 191 1471
Fragments arranged by size:
2175 1471 1135 681 219 191 99 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2583 2583
Size: 5977
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 2834 3304 1872
Size: 962 470 4545
Fragments arranged by size:
4545 962 470
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4081 4982 5244 820
Size: 197 926 2138 901 262 1553
Fragments arranged by size:
2138 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3335 1132
Size: 2203 3774
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2578 3148 3416 513
Size: 552 1513 570 268 3074
Fragments arranged by size:
3074 1513 570 552 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4004 4021 4174 4359 4804 325
Size: 1255 2424 17 153 185 445 1498
Fragments arranged by size:
2424 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2548 4223 5935
Size: 274 80 33 51 1443 571 1675 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1712 1675 1443 571 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2790 2822 5937 5969 2790
Size: 32 3115 32 2798
Fragments arranged by size:
3115 2798 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 2832 3302 1870
Size: 962 470 4545
Fragments arranged by size:
4545 962 470
XmnI GAAnn'nnTTC
Cuts at: 2094 2745 4106 2094
Size: 651 1361 3965
Fragments arranged by size:
3965 1361 651
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfoI SrfI SwaI XcmI