Restriction map for
pFN21AE4407
(Circular) (Six-base) MAPSORT of: pFN21AE4407.seq Check: 9463 from: 1 to: 5968
pFN21AE4407 5968 bp
With 182 enzymes: SgfI *
March 28, 2012 13:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2546 2555 4031 5415 5683 1632
Size: 467 447 9 1476 1384 268 1917
Fragments arranged by size:
1917 1476 1384 467 447 268 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 4867 5844 1203
Size: 3664 977 1327
Fragments arranged by size:
3664 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 2681 2953 4697 2681
Size: 272 1744 3952
Fragments arranged by size:
3952 1744 272
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3230 1184
Size: 2046 3922
AlwNI CAG_nnn'CTG
Cuts at: 2923 3711 5283 2923
Size: 788 1572 3608
Fragments arranged by size:
3608 1572 788
ApaI G_GGCC'C
Cuts at: 2899 2899
Size: 5968
ApaLI G'TGCA_C
Cuts at: 4025 5181 4025
Size: 1156 4812
ApoI r'AATT_y
Cuts at: 1493 2355 3277 3503 3655 5764 1493
Size: 862 922 226 152 2109 1697
Fragments arranged by size:
2109 1697 922 862 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2280 2792 2898 3293 1870
Size: 73 337 512 106 395 4545
Fragments arranged by size:
4545 512 395 337 106 73
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 3242 3289 4744 618
Size: 325 217 627 447 1008 47 1455 1842
Fragments arranged by size:
1842 1455 1008 627 447 325 217 47
BanII G_rGCy'C
Cuts at: 1391 2454 2600 2700 2899 3287 1391
Size: 1063 146 100 199 388 4072
Fragments arranged by size:
4072 1063 388 199 146 100
BbeI G_GCGC'C
Cuts at: 3246 3246
Size: 5968
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2620 928
Size: 971 721 4276
Fragments arranged by size:
4276 971 721
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4159 4193 1903
Size: 34 2222 34 3678
Fragments arranged by size:
3678 2222 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 5070 3860
Size: 1210 4758
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4579 136
Size: 107 122 71 631 605 2907 1525
Fragments arranged by size:
2907 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2049 5963 2049
Size: 3914 2054
BlpI GC'TnA_GC
Cuts at: 3104 3385 3104
Size: 281 5687
Bme1580I G_kGCm'C
Cuts at: 1790 2482 2899 3219 4029 5185 1790
Size: 692 417 320 810 1156 2573
Fragments arranged by size:
2573 1156 810 692 417 320
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2901 3699 4657
Size: 416 53 83 399 24 1484 798 958
Cuts at: 4657 442
Size: 1753
Fragments arranged by size:
1753 1484 958 798 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2417 2567 4628 1239
Size: 1178 150 2061 2579
Fragments arranged by size:
2579 2061 1178 150
Bpu10I CC'TnA_GC
Cuts at: 2094 2094
Size: 5968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2471 4093 4958 5256 2471
Size: 1622 865 298 3183
Fragments arranged by size:
3183 1622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 2731 4631 882
Size: 617 1098 134 1900 2219
Fragments arranged by size:
2219 1900 1098 617 134
BsaAI yAC'GTr
Cuts at: 493 1204 3759 493
Size: 711 2555 2702
Fragments arranged by size:
2702 2555 711
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3243 3775 4157 4792
Size: 53 83 186 936 1710 532 382 635
Cuts at: 4792 275
Size: 1451
Fragments arranged by size:
1710 1451 936 635 532 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4401 4799 5073 5220 5631 4401
Size: 398 274 147 411 4738
Fragments arranged by size:
4738 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5743
Fragments arranged by size:
5743 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2298 2312 2816 3104 1186
Size: 1112 14 504 288 4050
Fragments arranged by size:
4050 1112 504 288 14
BseYI C'CCAG_C
Cuts at: 2481 2641 5171 5667 2481
Size: 160 2530 496 2782
Fragments arranged by size:
2782 2530 496 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3030 3050 3241 1163
Size: 573 1294 20 191 3890
Fragments arranged by size:
3890 1294 573 191 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3418 4179 5207 1540
Size: 275 186 1417 761 1028 2301
Fragments arranged by size:
2301 1417 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2600 4029 4114 5185 2600
Size: 1429 85 1071 3383
Fragments arranged by size:
3383 1429 1071 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2454 2482 2600 2700 2899 3219 3287
Size: 399 664 28 118 100 199 320 68
Cuts at: 3287 4029 4114 5185 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 399 320 199 118
100 85 68 28
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2979 3323 844
Size: 2135 344 3489
Fragments arranged by size:
3489 2135 344
BsrBI CCG'CTC
Cuts at: 1394 1823 2566 3855 1394
Size: 429 743 1289 3507
Fragments arranged by size:
3507 1289 743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2755 3139 4463 4637 65
Size: 1183 1507 384 1324 174 1396
Fragments arranged by size:
1507 1396 1324 1183 384 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2618 4612 4799 1700
Size: 110 808 1994 187 2869
Fragments arranged by size:
2869 1994 808 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5968
BssSI C'ACGA_G
Cuts at: 4028 5040 4028
Size: 1012 4956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2923 794
Size: 2129 3839
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 2719 1184
Size: 1535 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 3166 3166
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 2049 3298 3665 4050 4067 5519 5605 5617
Size: 77 1249 367 385 17 1452 86 12
Cuts at: 5617 5963 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1249 385 367 346 86 77
17 12
Bsu36I CC'TnA_GG
Cuts at: 2074 2074
Size: 5968
BtgI C'CryG_G
Cuts at: 513 1065 2975 513
Size: 552 1910 3506
Fragments arranged by size:
3506 1910 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 3484 4278 4298 3484
Size: 794 20 5154
Fragments arranged by size:
5154 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2415 2620
Size: 54 1425 50 275 18 303 282 205
Cuts at: 2620 2933 3156 3415 4304 5933 8
Size: 313 223 259 889 1629 43
Fragments arranged by size:
1629 1425 889 313 303 282 275 259
223 205 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3415 1537
Size: 275 1603 4090
Fragments arranged by size:
4090 1603 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2588 2631 3898 1353
Size: 812 423 43 1267 3423
Fragments arranged by size:
3423 1267 812 423 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4555 4929 5075 387
Size: 1064 138 2966 374 146 1280
Fragments arranged by size:
2966 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2598 2598
Size: 5968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2417 2546 2555 2567 4031 4628
Size: 393 467 318 129 9 12 1464 597
Cuts at: 4628 5415 5683 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1464 787 597 467 393 318 268
129 12 9
EcoO109I rG'GnC_Cy
Cuts at: 2320 2895 2320
Size: 575 5393
EcoRI G'AATT_C
Cuts at: 2355 3277 3655 2355
Size: 922 378 4668
Fragments arranged by size:
4668 922 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2176 2731 2071
Size: 105 555 5308
Fragments arranged by size:
5308 555 105
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 2280 3142 3246 5115 1678
Size: 602 862 104 1869 2531
Fragments arranged by size:
2531 1869 862 602 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2667 2699 2939 2971 4609
Size: 32 135 32 1095 32 240 32 1638
Cuts at: 4609 4641 4683 4715 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1638 1095 240 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3558 669
Size: 988 281 1374 246 3079
Fragments arranged by size:
3079 1374 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KasI G'GCGC_C
Cuts at: 3242 3242
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5082 5266 1305
Size: 3777 184 2007
Fragments arranged by size:
3777 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 3158 10
Size: 54 1425 343 303 1023 2820
Fragments arranged by size:
2820 1425 1023 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 3926 4285 4444 518
Size: 648 1074 1686 359 159 2042
Fragments arranged by size:
2042 1686 1074 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2509 2977 3014 3384 4061 5209 5454 1606
Size: 903 468 37 370 677 1148 245 2120
Fragments arranged by size:
2120 1148 903 677 468 370 245 37
NaeI GCC'GGC
Cuts at: 2620 2620
Size: 5968
NarI GG'CG_CC
Cuts at: 3243 3243
Size: 5968
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5416
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NgoMIV G'CCGG_C
Cuts at: 2618 2618
Size: 5968
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 3326 4871 1132
Size: 97 2097 1545 2229
Fragments arranged by size:
2229 2097 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2687 2700 1348
Size: 1339 13 4616
Fragments arranged by size:
4616 1339 13
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5968
PfoI T'CCnGG_A
Cuts at: 2631 2631
Size: 5968
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 2320
Size: 5968
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PspOMI G'GGCC_C
Cuts at: 2895 2895
Size: 5968
PspXI vC'TCGA_Gb
Cuts at: 2280 2280
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2103 2928 3320 830
Size: 1273 825 392 3478
Fragments arranged by size:
3478 1273 825 392
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 2509 3014 2509
Size: 505 5463
SacI G_AGCT'C
Cuts at: 2600 2600
Size: 5968
SacII CC_GC'GG
Cuts at: 2978 2978
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SapI GCTCTTCn'nnn_
Cuts at: 2165 2588 2631 2165
Size: 423 43 5502
Fragments arranged by size:
5502 423 43
SbfI CC_TGCA'GG
Cuts at: 3320 3320
Size: 5968
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SexAI A'CCwGG_T
Cuts at: 2433 2433
Size: 5968
SfcI C'TryA_G
Cuts at: 826 1045 2099 2924 3316 4451 5132 5323 826
Size: 219 1054 825 392 1135 681 191 1471
Fragments arranged by size:
1471 1135 1054 825 681 392 219 191
SfoI GGC'GCC
Cuts at: 3244 3244
Size: 5968
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 2794 2900 3295 1872
Size: 922 106 395 4545
Fragments arranged by size:
4545 922 395 106
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2280 2486 4072 4973 5235 820
Size: 197 926 337 206 1586 901 262 1553
Fragments arranged by size:
1586 1553 926 901 337 262 206 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 2657 2846 1517
Size: 1140 189 4639
Fragments arranged by size:
4639 1140 189
StyI C'CwwG_G
Cuts at: 513 1065 2082 2841 3126 3407 513
Size: 552 1017 759 285 281 3074
Fragments arranged by size:
3074 1017 759 552 285 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2896 2974 3995 4012 4165 4350 4795
Size: 1255 1316 78 1021 17 153 185 445
Cuts at: 4795 325
Size: 1498
Fragments arranged by size:
1498 1316 1255 1021 445 185 153 78
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2301 4214 5926
Size: 274 80 33 51 1443 324 1913 1712
Cuts at: 5926 96
Size: 138
Fragments arranged by size:
1913 1712 1443 324 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5928 5960 5928
Size: 32 5936
Tth111I GACn'n_nGTC
Cuts at: 1316 2717 1316
Size: 1401 4567
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 2707
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 2280 1943
Size: 337 5631
XmaI C'CCGG_G
Cuts at: 1870 2792 2898 3293 1870
Size: 922 106 395 4545
Fragments arranged by size:
4545 922 395 106
XmnI GAAnn'nnTTC
Cuts at: 2555 4097 2555
Size: 1542 4426
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SanDI
SfiI SrfI SwaI