Restriction map for
pFN21AE4396
(Circular) (Six-base) MAPSORT of: pFN21AE4396.seq Check: 6526 from: 1 to: 5956
pFN21AE4396 5956 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2378 2378
Size: 5956
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2091 3766 278
Size: 53 83 186 936 555 1675 2468
Fragments arranged by size:
2468 1675 936 555 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 2769 3277 2769
Size: 508 5448
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4019 5403 5671 1632
Size: 2387 1384 268 1917
Fragments arranged by size:
2387 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 2853 4685 2853
Size: 1832 4124
AleI CACnn'nnGTG
Cuts at: 1166 2111 1166
Size: 945 5011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2967 2999 3209 3241 2967
Size: 32 210 32 5682
Fragments arranged by size:
5682 210 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2606 2619 2704 2896 3699 5271 2606
Size: 13 85 192 803 1572 3291
Fragments arranged by size:
3291 1572 803 192 85 13
ApaI G_GGCC'C
Cuts at: 2255 2255
Size: 5956
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2282 3265 3491 3643 5752 1493
Size: 789 983 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 789 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 3151 3281 1870
Size: 73 67 1141 130 4545
Fragments arranged by size:
4545 1141 130 73 67
AvrII C'CTAG_G
Cuts at: 2956 2956
Size: 5956
BamHI G'GATC_C
Cuts at: 3232 3286 3653 3232
Size: 54 367 5535
Fragments arranged by size:
5535 367 54
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2113 2161 2578 2629
Size: 325 217 627 314 12 48 417 51
Cuts at: 2629 2769 2885 3277 4732 618
Size: 140 116 392 1455 1842
Fragments arranged by size:
1842 1455 627 417 392 325 314 217
140 116 51 48 12
BanII G_rGCy'C
Cuts at: 1391 2255 2826 2965 3275 1391
Size: 864 571 139 310 4072
Fragments arranged by size:
4072 864 571 310 139
BbeI G_GCGC'C
Cuts at: 2105 2889 2105
Size: 784 5172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2227 928
Size: 971 328 4657
Fragments arranged by size:
4657 971 328
BbvCI CC'TCA_GC
Cuts at: 2327 2451 2697 3175 2327
Size: 124 246 478 5108
Fragments arranged by size:
5108 478 246 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2560 2594 4147 4181 1903
Size: 34 623 34 1553 34 3678
Fragments arranged by size:
3678 1553 623 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2752 3848 5058 2752
Size: 1096 1210 3650
Fragments arranged by size:
3650 1210 1096
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2118 2166 2255 2490 2634 3251 4017 5173
Size: 328 48 89 235 144 617 766 1156
Cuts at: 5173 1790
Size: 2573
Fragments arranged by size:
2573 1156 766 617 328 235 144 89
48
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 3000 3687
Size: 416 53 83 399 24 892 691 687
Cuts at: 3687 4645 442
Size: 958 1753
Fragments arranged by size:
1753 958 892 691 687 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2496 4616 1239
Size: 1257 2120 2579
Fragments arranged by size:
2579 2120 1257
Bpu10I CC'TnA_GC
Cuts at: 2327 2451 2697 3175 2327
Size: 124 246 478 5108
Fragments arranged by size:
5108 478 246 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2620 4081 4946 5244 2620
Size: 1461 865 298 3332
Fragments arranged by size:
3332 1461 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4619 882
Size: 617 3120 2219
Fragments arranged by size:
3120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2102 2886 3763
Size: 53 83 186 936 555 14 784 877
Cuts at: 3763 4145 4780 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 877 784 635 555 382 186
83 53 14
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2469 2499 1178
Size: 30 165 30 1066 30 4635
Fragments arranged by size:
4635 1066 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5956
BseYI C'CCAG_C
Cuts at: 2255 2889 3079 5159 5655 2255
Size: 634 190 2080 496 2556
Fragments arranged by size:
2556 2080 634 496 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 1163
Size: 573 381 5002
Fragments arranged by size:
5002 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2679 2846 2965 3015 4017 4102 5173 2679
Size: 167 119 50 1002 85 1071 3462
Fragments arranged by size:
3462 1071 1002 167 119 85 50
BsmI GAATG_Cn'
Cuts at: 2323 3467 3560 2323
Size: 1144 93 4719
Fragments arranged by size:
4719 1144 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2509 2509
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2166 2255 2490 2634 2679 2826
Size: 399 328 48 89 235 144 45 147
Cuts at: 2826 2846 2965 3015 3251 3275 4017 4102 5173
Size: 20 119 50 236 24 742 85 1071
Cuts at: 5173 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 328 236 235 147
144 119 89 85 50 48 45 24
20
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2378 2672 2807 3311 844
Size: 1534 294 135 504 3489
Fragments arranged by size:
3489 1534 504 294 135
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2678 4016 5028 2678
Size: 1338 1012 3606
Fragments arranged by size:
3606 1338 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2278 2474 2278
Size: 196 5760
BstYI r'GATC_y
Cuts at: 1972 3232 3286 3653 4038 4055 5507 5593 5605
Size: 1260 54 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1260 385 367 346 86 54
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2490 2850 513
Size: 552 939 12 474 360 3619
Fragments arranged by size:
3619 939 552 474 360 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2817 487
Size: 683 56 1591 3626
Fragments arranged by size:
3626 1591 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2625 2660 529
Size: 35 2061 35 3825
Fragments arranged by size:
3825 2061 35 35
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2185 4963 809
Size: 507 869 2778 1802
Fragments arranged by size:
2778 1802 869 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2030 2066
Size: 54 1425 50 275 18 189 11 36
Cuts at: 2066 2229 2776 3403 4292 5921 8
Size: 163 547 627 889 1629 43
Fragments arranged by size:
1629 1425 889 627 547 275 189 163
54 50 43 36 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3229 3886 1353
Size: 1876 657 3423
Fragments arranged by size:
3423 1876 657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2963 2963
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2496 4019 4616 5403 5671 1239
Size: 393 864 1523 597 787 268 1524
Fragments arranged by size:
1524 1523 864 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2326 2326
Size: 5956
EcoO109I rG'GnC_Cy
Cuts at: 2251 2252 2855 2999 3124 2251
Size: 1 603 144 125 5083
Fragments arranged by size:
5083 603 144 125 1
EcoRI G'AATT_C
Cuts at: 2282 3265 3643 2282
Size: 983 378 4595
Fragments arranged by size:
4595 983 378
EcoRV GAT'ATC
Cuts at: 2413 2413
Size: 5956
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2051 2105 2889 3174 5103 1678
Size: 373 54 784 285 1929 2531
Fragments arranged by size:
2531 1929 784 373 285 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2839 2871 4597 4629 4671
Size: 32 135 32 1267 32 1726 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 1726 1267 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KasI G'GCGC_C
Cuts at: 2101 2885 2101
Size: 784 5172
KpnI G_GTAC'C
Cuts at: 2773 3281 2773
Size: 508 5448
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2068 2778 10
Size: 54 1425 343 236 710 3188
Fragments arranged by size:
3188 1425 710 343 236 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 2174 2849 3914 4273 4432 518
Size: 648 945 63 675 1065 359 159 2042
Fragments arranged by size:
2042 1065 945 675 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2035 2194 2450 2499 2701 3203 3372 4049
Size: 429 159 256 49 202 502 169 677
Cuts at: 4049 5197 5442 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 502 429 256 245 202
169 159 49
NarI GG'CG_CC
Cuts at: 2102 2886 2102
Size: 784 5172
NcoI C'CATG_G
Cuts at: 513 1065 2004 2016 2490 2850 513
Size: 552 939 12 474 360 3619
Fragments arranged by size:
3619 939 552 474 360 12
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 2186 3025 3167 3191 3314 4859 1132
Size: 97 957 839 142 24 123 1545 2229
Fragments arranged by size:
2229 1545 957 839 142 123 97 24
PasI CC'CwG_GG
Cuts at: 1348 2881 1348
Size: 1533 4423
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2619 1108
Size: 1511 4445
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2855 2999 3124 2855
Size: 144 125 5687
Fragments arranged by size:
5687 144 125
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2251 2251
Size: 5956
PspXI vC'TCGA_Gb
Cuts at: 3151 3151
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2194 2450 2701 3203 2194
Size: 256 251 502 4947
Fragments arranged by size:
4947 502 256 251
SacI G_AGCT'C
Cuts at: 2965 2965
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SanDI GG'GwC_CC
Cuts at: 2855 2855
Size: 5956
SapI GCTCTTCn'nnn_
Cuts at: 3229 3229
Size: 5956
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 2156 3304 4439 5120 5311 826
Size: 219 1111 1148 1135 681 191 1471
Fragments arranged by size:
1471 1148 1135 1111 681 219 191
SfoI GGC'GCC
Cuts at: 2103 2887 2103
Size: 784 5172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2635 3151 4060 4961 5223 820
Size: 197 926 692 516 909 901 262 1553
Fragments arranged by size:
1553 926 909 901 692 516 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3167 3314 1132
Size: 2035 147 3774
Fragments arranged by size:
3774 2035 147
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2490 2773 2850 2956 3395
Size: 552 939 12 474 283 77 106 439
Cuts at: 3395 513
Size: 3074
Fragments arranged by size:
3074 939 552 474 439 283 106 77
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 3983 4000 4153 4338 4783 325
Size: 1255 543 1860 17 153 185 445 1498
Fragments arranged by size:
1860 1498 1255 543 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 3155 4202
Size: 274 80 33 51 1443 747 431 1047
Cuts at: 4202 5914 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1047 747 431 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 5916 5948 2469
Size: 32 3415 32 2477
Fragments arranged by size:
3415 2477 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2090 1316
Size: 774 5182
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3043 3043
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 3151 1943
Size: 1208 4748
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 4085 4085
Size: 5956
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2089 3764 276
Size: 53 83 186 936 555 1675 2468
Fragments arranged by size:
2468 1675 936 555 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PsrI RsrII SacII SexAI SfiI SrfI
SwaI