Restriction map for
pFN21AE4393
(Circular) (Six-base) MAPSORT of: pFN21AE4393.seq Check: 3880 from: 1 to: 5950
pFN21AE4393 5950 bp
With 182 enzymes: SgfI *
March 28, 2012 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2094 2493 2891 3111 3129 4013 5397 5665
Size: 462 399 398 220 18 884 1384 268
Cuts at: 5665 1632
Size: 1917
Fragments arranged by size:
1917 1384 884 462 399 398 268 220
18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 3075 1166
Size: 1909 4041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2789 1184
Size: 1605 4345
AlwNI CAG_nnn'CTG
Cuts at: 2347 3693 5265 2347
Size: 1346 1572 3032
Fragments arranged by size:
3032 1572 1346
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 2362 3259 3485 3637 5746 1493
Size: 869 897 226 152 2109 1697
Fragments arranged by size:
2109 1697 897 869 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2139 4504 160
Size: 872 1107 2365 1606
Fragments arranged by size:
2365 1606 1107 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3271 4726 618
Size: 325 217 627 1484 1455 1842
Fragments arranged by size:
1842 1484 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3269 1391
Size: 1878 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2670 928
Size: 971 771 4208
Fragments arranged by size:
4208 971 771
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2633 3059 4561
Size: 107 122 71 631 605 961 426 1502
Cuts at: 4561 136
Size: 1525
Fragments arranged by size:
1525 1502 961 631 605 426 122 107
71
BglII A'GATC_T
Cuts at: 3170 5945 3170
Size: 2775 3175
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2712 4011 5167 1790
Size: 922 1299 1156 2573
Fragments arranged by size:
2573 1299 1156 922
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 3681 4639
Size: 416 53 83 399 24 1206 1058 958
Cuts at: 4639 442
Size: 1753
Fragments arranged by size:
1753 1206 1058 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4610 1239
Size: 3371 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4075 4940 5238 2564
Size: 1511 865 298 3276
Fragments arranged by size:
3276 1511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2177 2279 4613 882
Size: 617 678 102 2334 2219
Fragments arranged by size:
2334 2219 678 617 102
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2166 4383 4781 5055 5202 5613 2166
Size: 2217 398 274 147 411 2503
Fragments arranged by size:
2503 2217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2296 2326 1178
Size: 30 165 30 893 30 4802
Fragments arranged by size:
4802 893 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5950
BseYI C'CCAG_C
Cuts at: 5153 5649 5153
Size: 496 5454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2749 3053 1163
Size: 573 1013 304 4060
Fragments arranged by size:
4060 1013 573 304
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4011 4096 5167 4011
Size: 85 1071 4794
Fragments arranged by size:
4794 1071 85
BsmI GAATG_Cn'
Cuts at: 2153 2800 2976 3461 3554 2153
Size: 647 176 485 93 4549
Fragments arranged by size:
4549 647 485 176 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2712 3269 4011 4096 5167 1391
Size: 399 922 557 742 85 1071 2174
Fragments arranged by size:
2174 1071 922 742 557 399 85
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3837 1394
Size: 429 2014 3507
Fragments arranged by size:
3507 2014 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4594 4781 1700
Size: 110 2784 187 2869
Fragments arranged by size:
2869 2784 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstYI r'GATC_y
Cuts at: 1972 2325 3170 3280 3647 4032 4049 5501 5587
Size: 353 845 110 367 385 17 1452 86
Cuts at: 5587 5599 5945 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 845 385 367 353 346 110
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 3466 4260 4280 3466
Size: 794 20 5136
Fragments arranged by size:
5136 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 2623 3254 2623
Size: 631 5319
DraIII CAC_nnn'GTG
Cuts at: 2746 2746
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3397 4286 5915
Size: 54 1425 50 275 18 1567 889 1629
Cuts at: 5915 8
Size: 43
Fragments arranged by size:
1629 1567 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 3880 1353
Size: 1058 1469 3423
Fragments arranged by size:
3423 1469 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2094 2493 2891 3111 3129 4013 4610
Size: 393 462 399 398 220 18 884 597
Cuts at: 4610 5397 5665 1239
Size: 787 268 1524
Fragments arranged by size:
1524 884 787 597 462 399 398 393
268 220 18
EcoO109I rG'GnC_Cy
Cuts at: 2253 2253
Size: 5950
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 3134 2218
Size: 916 5034
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 5097 1678
Size: 3419 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2544 4591 4623 4665
Size: 32 135 32 940 32 2047 32 42
Cuts at: 4665 4697 1373
Size: 32 2626
Fragments arranged by size:
2626 2047 940 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2908 3294 3540 669
Size: 988 281 970 386 246 3079
Fragments arranged by size:
3079 988 970 386 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 5064 5248 1305
Size: 1215 2544 184 2007
Fragments arranged by size:
2544 2007 1215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4128
Fragments arranged by size:
4128 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2594 3075 3908 4267 4426 518
Size: 648 1149 279 481 833 359 159 2042
Fragments arranged by size:
2042 1149 833 648 481 359 279 159
MspA1I CmG'CkG
Cuts at: 1606 3101 3366 4043 5191 5436 1606
Size: 1495 265 677 1148 245 2120
Fragments arranged by size:
2120 1495 1148 677 265 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5398
NdeI CA'TA_TG
Cuts at: 387 2944 387
Size: 2557 3393
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2987 3308 4853 1132
Size: 97 1758 321 1545 2229
Fragments arranged by size:
2229 1758 1545 321 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2474 3030 1613
Size: 861 556 4533
Fragments arranged by size:
4533 861 556
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2156 2188 2156
Size: 32 5918
PstI C_TGCA'G
Cuts at: 830 2230 2264 2509 3100 3302 830
Size: 1400 34 245 591 202 3478
Fragments arranged by size:
3478 1400 591 245 202 34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 3101 3101
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 2226 2260 2505 2785 2891 3096 3298
Size: 219 1181 34 245 280 106 205 202
Cuts at: 3298 4433 5114 5305 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1181 1135 681 280 245 219 205
202 191 106 34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2579 4054 4955 5217 820
Size: 197 926 636 1475 901 262 1553
Fragments arranged by size:
1553 1475 926 901 636 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 2987 3308 1132
Size: 1855 321 3774
Fragments arranged by size:
3774 1855 321
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2289 2820 2950 3389 513
Size: 552 1224 531 130 439 3074
Fragments arranged by size:
3074 1224 552 531 439 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2282 3977 3994 4147 4332 4777 325
Size: 1255 702 1695 17 153 185 445 1498
Fragments arranged by size:
1695 1498 1255 702 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2296 2328 5910 5942 2296
Size: 32 3582 32 2304
Fragments arranged by size:
3582 2304 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 2201 4079 2201
Size: 1878 4072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI