Restriction map for
pFN21AE4392
(Circular) (Six-base) MAPSORT of: pFN21AE4392.seq Check: 6372 from: 1 to: 5950
pFN21AE4392 5950 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 3062 3271 3062
Size: 209 5741
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2604 3037 4013 5397 5665 1632
Size: 972 433 976 1384 268 1917
Fragments arranged by size:
1917 1384 976 972 433 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 2585 1166
Size: 1419 4531
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 2046 2173 2969 3693 5265 2046
Size: 127 796 724 1572 2731
Fragments arranged by size:
2731 1572 796 724 127
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2714 3062 3271 4726 618
Size: 325 217 627 927 348 209 1455 1842
Fragments arranged by size:
1842 1455 927 627 348 325 217 209
BanII G_rGCy'C
Cuts at: 1391 2609 2814 2943 3269 1391
Size: 1218 205 129 326 4072
Fragments arranged by size:
4072 1218 326 205 129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2680 2986 928
Size: 971 781 306 3892
Fragments arranged by size:
3892 971 781 306
BbvCI CC'TCA_GC
Cuts at: 2286 2699 2286
Size: 413 5537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2092 2126 2257 2291 4141 4175 1903
Size: 34 155 34 131 34 1850 34 3678
Fragments arranged by size:
3678 1850 155 131 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 2707 1548
Size: 1159 4791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3108 4561 136
Size: 107 122 71 631 605 1436 1453 1525
Fragments arranged by size:
1525 1453 1436 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2270 4011 5167 1790
Size: 480 1741 1156 2573
Fragments arranged by size:
2573 1741 1156 480
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 3099 3681
Size: 416 53 83 399 24 602 1080 582
Cuts at: 3681 4639 442
Size: 958 1753
Fragments arranged by size:
1753 1080 958 602 582 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3232 3232
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2566 2693 3152 4610 1239
Size: 1327 127 459 1458 2579
Fragments arranged by size:
2579 1458 1327 459 127
Bpu10I CC'TnA_GC
Cuts at: 2286 2699 2937 2286
Size: 413 238 5299
Fragments arranged by size:
5299 413 238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2373 4613 882
Size: 617 874 2240 2219
Fragments arranged by size:
2240 2219 874 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 2784 5944 2784
Size: 3160 2790
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4383 4781 5055 5202 5613 4383
Size: 398 274 147 411 4720
Fragments arranged by size:
4720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2242 2272 2353 2383 2958
Size: 30 165 30 839 30 81 30 575
Cuts at: 2958 2988 1178
Size: 30 4140
Fragments arranged by size:
4140 839 575 165 81 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2687 3167 1186
Size: 1501 480 3969
Fragments arranged by size:
3969 1501 480
BseYI C'CCAG_C
Cuts at: 2021 2046 3007 3165 5153 5649 2021
Size: 25 961 158 1988 496 2322
Fragments arranged by size:
2322 1988 961 496 158 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 1163
Size: 573 450 4927
Fragments arranged by size:
4927 573 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2482 2552 2814 2943 4011 4096 5167 2482
Size: 70 262 129 1068 85 1071 3265
Fragments arranged by size:
3265 1071 1068 262 129 85 70
BsmI GAATG_Cn'
Cuts at: 2801 3461 3554 2801
Size: 660 93 5197
Fragments arranged by size:
5197 660 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2482 2552 2609 2814 2943 3269
Size: 399 480 212 70 57 205 129 326
Cuts at: 3269 4011 4096 5167 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 326 212 205
129 85 70 57
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2205 2245 2677 3837 1394
Size: 429 382 40 432 1160 3507
Fragments arranged by size:
3507 1160 432 429 382 40
BsrDI GCAATG_nn'
Cuts at: 65 1248 3064 4445 4619 65
Size: 1183 1816 1381 174 1396
Fragments arranged by size:
1816 1396 1381 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2489 4594 4781 1700
Size: 110 679 2105 187 2869
Fragments arranged by size:
2869 2105 679 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 2942 4010 5022 2942
Size: 1068 1012 3870
Fragments arranged by size:
3870 1068 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5950
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 2176 1184
Size: 992 4958
BstYI r'GATC_y
Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945
Size: 1308 367 385 17 1452 86 12 346
Cuts at: 5945 1972
Size: 1977
Fragments arranged by size:
1977 1452 1308 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2054 2181 2261 2346 2633 2662 3219
Size: 552 989 127 80 85 287 29 557
Cuts at: 3219 513
Size: 3244
Fragments arranged by size:
3244 989 557 552 287 127 85 80
29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2314 2451 2718 3058 487
Size: 683 56 1088 137 267 340 3379
Fragments arranged by size:
3379 1088 683 340 267 137 56
BtsI GCAGTG_nn'
Cuts at: 2860 2866 3466 4260 4280 2860
Size: 6 600 794 20 4530
Fragments arranged by size:
4530 794 600 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2177 2212 529
Size: 35 1613 35 4267
Fragments arranged by size:
4267 1613 35 35
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2659 3095 3222
Size: 54 1425 50 275 18 829 436 127
Cuts at: 3222 3397 4286 5915 8
Size: 175 889 1629 43
Fragments arranged by size:
1629 1425 889 829 436 275 175 127
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2277 3880 1353
Size: 924 1603 3423
Fragments arranged by size:
3423 1603 924
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3088 4537 4911 5057 387
Size: 1064 138 1499 1449 374 146 1280
Fragments arranged by size:
1499 1449 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2812 2941 2812
Size: 129 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2566 2604 2693 3037 3152 4013 4610
Size: 393 934 38 89 344 115 861 597
Cuts at: 4610 5397 5665 1239
Size: 787 268 1524
Fragments arranged by size:
1524 934 861 787 597 393 344 268
115 89 38
EcoO109I rG'GnC_Cy
Cuts at: 2913 3101 3160 2913
Size: 188 59 5703
Fragments arranged by size:
5703 188 59
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 2033 2728 5097 1678
Size: 355 695 2369 2531
Fragments arranged by size:
2531 2369 695 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2958 2990 4591 4623 4665
Size: 32 135 32 1386 32 1601 32 42
Cuts at: 4665 4697 1373
Size: 32 2626
Fragments arranged by size:
2626 1601 1386 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3066 3275 3066
Size: 209 5741
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3027 5064 5248 1305
Size: 1722 2037 184 2007
Fragments arranged by size:
2037 2007 1722 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2661 3097 3224 10
Size: 54 1425 343 829 436 127 2736
Fragments arranged by size:
2736 1425 829 436 343 127 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 3218 3908 4267 4426 518
Size: 648 1419 633 690 359 159 2042
Fragments arranged by size:
2042 1419 690 648 633 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2173 2263 2626 2745 3366 4043 5191
Size: 450 117 90 363 119 621 677 1148
Cuts at: 5191 5436 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 621 450 363 245 119
117 90
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 5950
NcoI C'CATG_G
Cuts at: 513 1065 2181 2346 2662 513
Size: 552 1116 165 316 3801
Fragments arranged by size:
3801 1116 552 316 165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 3308 4853 1132
Size: 97 2079 1545 2229
Fragments arranged by size:
2229 2079 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 2034 2543 2034
Size: 509 5441
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2157 1613
Size: 544 5406
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2965 3302 830
Size: 2135 337 3478
Fragments arranged by size:
3478 2135 337
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
SacI G_AGCT'C
Cuts at: 2814 2943 2814
Size: 129 5821
SacII CC_GC'GG
Cuts at: 2057 2264 2057
Size: 207 5743
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 2080 2373 2961 2970 3298 4433 5114
Size: 219 1035 293 588 9 328 1135 681
Cuts at: 5114 5305 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1035 681 588 328 293 219
191 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2139 2181 2346 2419 2662 3389 513
Size: 552 1074 42 165 73 243 727 3074
Fragments arranged by size:
3074 1074 727 552 243 165 73 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2080 2147 2188 3977 3994 4147 4332
Size: 1255 500 67 41 1789 17 153 185
Cuts at: 4332 4777 325
Size: 445 1498
Fragments arranged by size:
1789 1498 1255 500 445 185 153 67
41 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4196 5908 96
Size: 274 80 33 51 1443 2219 1712 138
Fragments arranged by size:
2219 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2242 2274 2353 2385 5910 5942 2242
Size: 32 79 32 3525 32 2250
Fragments arranged by size:
3525 2250 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2684 1316
Size: 1368 4582
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 2153 4079 2153
Size: 1926 4024
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI