Restriction map for
pFN21AE4391
(Circular) (Six-base) MAPSORT of: pFN21AE4391.seq Check: 1613 from: 1 to: 5947
pFN21AE4391 5947 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2857 2857
Size: 5947
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2916 3290 1937
Size: 979 374 4594
Fragments arranged by size:
4594 979 374
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2299 2864 2981 3098 4010 5394 5662 1632
Size: 667 565 117 117 912 1384 268 1917
Fragments arranged by size:
1917 1384 912 667 565 268 117 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 2919 4846 5823 1203
Size: 1716 1927 977 1327
Fragments arranged by size:
1927 1716 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2624 1184
Size: 1440 4507
AlwNI CAG_nnn'CTG
Cuts at: 2434 3130 3690 5262 2434
Size: 696 560 1572 3119
Fragments arranged by size:
3119 1572 696 560
ApaLI G'TGCA_C
Cuts at: 3062 4004 5160 3062
Size: 942 1156 3849
Fragments arranged by size:
3849 1156 942
ApoI r'AATT_y
Cuts at: 1493 2509 3256 3482 3634 5743 1493
Size: 1016 747 226 152 2109 1697
Fragments arranged by size:
2109 1697 1016 747 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 2716 2948 3113 3268
Size: 325 217 627 483 446 232 165 155
Cuts at: 3268 4723 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 483 446 325 232 217
165 155
BanII G_rGCy'C
Cuts at: 1391 3266 1391
Size: 1875 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2054 2387 2972 928
Size: 971 155 333 585 3903
Fragments arranged by size:
3903 971 585 333 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2335 5942 2335
Size: 3607 2340
BlpI GC'TnA_GC
Cuts at: 3072 3364 3072
Size: 292 5655
Bme1580I G_kGCm'C
Cuts at: 1790 2275 2721 2951 3066 4008 5164 1790
Size: 485 446 230 115 942 1156 2573
Fragments arranged by size:
2573 1156 942 485 446 230 115
BmgBI CAC'GTC
Cuts at: 1318 2920 1318
Size: 1602 4345
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2714 2866 4607 1239
Size: 1475 152 1741 2579
Fragments arranged by size:
2579 1741 1475 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 2254 4072 4937 5235 2168
Size: 86 1818 865 298 2880
Fragments arranged by size:
2880 1818 865 298 86
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3754 4136 4771 275
Size: 53 83 186 936 2221 382 635 1451
Fragments arranged by size:
2221 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3116 4380 4778 5052 5199 5610 3116
Size: 1264 398 274 147 411 3453
Fragments arranged by size:
3453 1264 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 2919 2949 1178
Size: 30 165 30 1461 30 25 30 4176
Fragments arranged by size:
4176 1461 165 30 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 1186
Size: 850 5097
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3066 3124 3213 4008 4093 5164 3066
Size: 58 89 795 85 1071 3849
Fragments arranged by size:
3849 1071 795 89 85 58
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2275 2721 2951 3066 3124 3213 3266
Size: 399 485 446 230 115 58 89 53
Cuts at: 3266 4008 4093 5164 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 446 399 230 115
89 85 58 53
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 2341 3836 5566 2341
Size: 1495 1730 2722
Fragments arranged by size:
2722 1730 1495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2857 3302 844
Size: 2013 445 3489
Fragments arranged by size:
3489 2013 445
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 2366 4007 5019 2366
Size: 1641 1012 3294
Fragments arranged by size:
3294 1641 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5947
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2035 2035
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 2335 3277 3644 4029 4046 5498 5584 5596
Size: 363 942 367 385 17 1452 86 12
Cuts at: 5596 5942 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 942 385 367 363 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2560 3135 513
Size: 552 1495 575 3325
Fragments arranged by size:
3325 1495 575 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3020 487
Size: 683 56 1794 3414
Fragments arranged by size:
3414 1794 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 3251 3251
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 3394 4283
Size: 54 1425 50 275 18 195 1369 889
Cuts at: 4283 5912 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1369 889 275 195 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3218 3877 1353
Size: 1865 659 3423
Fragments arranged by size:
3423 1865 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 2714 2864 2866 2981 3098 4010
Size: 393 667 415 150 2 115 117 912
Cuts at: 4010 4607 5394 5662 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 912 787 667 597 415 393 268
150 117 115 2
EcoO109I rG'GnC_Cy
Cuts at: 2389 2389
Size: 5947
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2212 2092
Size: 120 5827
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2753 2785 2864 2896 3016
Size: 32 135 32 1181 32 79 32 120
Cuts at: 3016 3048 4588 4620 4662 4694 1373
Size: 32 1540 32 42 32 2626
Fragments arranged by size:
2626 1540 1181 135 120 79 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3232 3291 3537 669
Size: 988 281 1294 59 246 3079
Fragments arranged by size:
3079 1294 988 281 246 59
HindIII A'AGCT_T
Cuts at: 748 2097 748
Size: 1349 4598
HpaI GTT'AAC
Cuts at: 3232 3537 3232
Size: 305 5642
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 3546 3546
Size: 5947
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 10
Size: 54 1425 343 195 3930
Fragments arranged by size:
3930 1425 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2606 3905 4264 4423 518
Size: 648 1440 1299 359 159 2042
Fragments arranged by size:
2042 1440 1299 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3110 3363 4040 5188 5433 1606
Size: 1504 253 677 1148 245 2120
Fragments arranged by size:
2120 1504 1148 677 253 245
NcoI C'CATG_G
Cuts at: 513 1065 2560 3135 513
Size: 552 1495 575 3325
Fragments arranged by size:
3325 1495 575 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 3305 4850 1132
Size: 97 2076 1545 2229
Fragments arranged by size:
2229 2076 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2389 2389
Size: 5947
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 2094 3517 2094
Size: 1423 4524
PstI C_TGCA'G
Cuts at: 830 2439 3299 830
Size: 1609 860 3478
Fragments arranged by size:
3478 1609 860
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SapI GCTCTTCn'nnn_
Cuts at: 3218 3218
Size: 5947
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 2244 2435 3295 4430 5111 5302 826
Size: 219 1199 191 860 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 860 681 219 191 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2183 2233 4051 4952 5214 820
Size: 197 926 240 50 1818 901 262 1553
Fragments arranged by size:
1818 1553 926 901 262 240 197 50
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 2022 1517
Size: 505 5442
StyI C'CwwG_G
Cuts at: 513 1065 2084 2113 2139 2560 3135 3386 513
Size: 552 1019 29 26 421 575 251 3074
Fragments arranged by size:
3074 1019 575 552 421 251 29 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 3974 3991 4144 4329 4774 325
Size: 1255 678 1716 17 153 185 445 1498
Fragments arranged by size:
1716 1498 1255 678 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4193 5905 96
Size: 274 80 33 51 1443 2216 1712 138
Fragments arranged by size:
2216 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2853 2885 5907 5939 2853
Size: 32 3022 32 2861
Fragments arranged by size:
3022 2861 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2263 2020
Size: 243 5704
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 2219 4076 2219
Size: 1857 4090
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI