Restriction map for
pFN21AE4387
(Circular) (Six-base) MAPSORT of: pFN21AE4387.seq Check: 2862 from: 1 to: 5929
pFN21AE4387 5929 bp
With 182 enzymes: SgfI *
March 28, 2012 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2517 3272 1937
Size: 580 755 4594
Fragments arranged by size:
4594 755 580
Acc65I G'GTAC_C
Cuts at: 2733 3250 2733
Size: 517 5412
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2800 3075 3992 5376 5644 1632
Size: 1168 275 917 1384 268 1917
Fragments arranged by size:
1917 1384 1168 917 275 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 4828 5805 1203
Size: 3625 977 1327
Fragments arranged by size:
3625 1327 977
AgeI A'CCGG_T
Cuts at: 4760 4760
Size: 5929
AhdI GACnn_n'nnGTC
Cuts at: 4658 4658
Size: 5929
AleI CACnn'nnGTG
Cuts at: 1166 3045 1166
Size: 1879 4050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2843 1184
Size: 1659 4270
AlwNI CAG_nnn'CTG
Cuts at: 2884 3169 3672 5244 2884
Size: 285 503 1572 3569
Fragments arranged by size:
3569 1572 503 285
ApaI G_GGCC'C
Cuts at: 3123 3123
Size: 5929
ApaLI G'TGCA_C
Cuts at: 3986 5142 3986
Size: 1156 4773
ApoI r'AATT_y
Cuts at: 1493 2285 3065 3238 3464 3616 5725 1493
Size: 792 780 173 226 152 2109 1697
Fragments arranged by size:
2109 1697 792 780 226 173 152
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 3254 1870
Size: 73 1311 4545
Fragments arranged by size:
4545 1311 73
BamHI G'GATC_C
Cuts at: 3259 3626 3259
Size: 367 5562
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2370 2455 2733 2993
Size: 325 217 627 516 67 85 278 260
Cuts at: 2993 3250 4705 618
Size: 257 1455 1842
Fragments arranged by size:
1842 1455 627 516 325 278 260 257
217 85 67
BanII G_rGCy'C
Cuts at: 1391 3123 3248 1391
Size: 1732 125 4072
Fragments arranged by size:
4072 1732 125
BbeI G_GCGC'C
Cuts at: 2374 2374
Size: 5929
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2617 928
Size: 971 718 4240
Fragments arranged by size:
4240 971 718
BbvCI CC'TCA_GC
Cuts at: 2877 2877
Size: 5929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4120 4154 1903
Size: 34 2183 34 3678
Fragments arranged by size:
3678 2183 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3821 5031 3821
Size: 1210 4719
BclI T'GATC_A
Cuts at: 1548 2040 2184 2662 1548
Size: 492 144 478 4815
Fragments arranged by size:
4815 492 478 144
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4540 136
Size: 107 122 71 631 605 337 2531 1525
Fragments arranged by size:
2531 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5924 5924
Size: 5929
BlpI GC'TnA_GC
Cuts at: 3346 3346
Size: 5929
Bme1580I G_kGCm'C
Cuts at: 1790 2308 2460 2996 3123 3990 5146 1790
Size: 518 152 536 127 867 1156 2573
Fragments arranged by size:
2573 1156 867 536 518 152 127
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4618 442
Size: 416 53 83 399 24 2243 958 1753
Fragments arranged by size:
2243 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2966 3008 4589 1239
Size: 1727 42 1581 2579
Fragments arranged by size:
2579 1727 1581 42
Bpu10I CC'TnA_GC
Cuts at: 2877 2877
Size: 5929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4054 4919 5217 4054
Size: 865 298 4766
Fragments arranged by size:
4766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4592 882
Size: 617 3093 2219
Fragments arranged by size:
3093 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3720 493
Size: 711 2516 2702
Fragments arranged by size:
2702 2516 711
BsaBI GATnn'nnATC
Cuts at: 5923 5923
Size: 5929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2371 3736 4118 4753
Size: 53 83 186 936 838 1365 382 635
Cuts at: 4753 275
Size: 1451
Fragments arranged by size:
1451 1365 936 838 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4362 4760 5034 5181 5592 4362
Size: 398 274 147 411 4699
Fragments arranged by size:
4699 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2394 2424 1178
Size: 30 165 30 991 30 4683
Fragments arranged by size:
4683 991 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 1186
Size: 1216 4713
BseYI C'CCAG_C
Cuts at: 2062 5132 5628 2062
Size: 3070 496 2363
Fragments arranged by size:
3070 2363 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3379 4140 5168 1540
Size: 275 186 1378 761 1028 2301
Fragments arranged by size:
2301 1378 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3990 4075 5146 3990
Size: 85 1071 4773
Fragments arranged by size:
4773 1071 85
BsmI GAATG_Cn'
Cuts at: 2270 3440 3533 2270
Size: 1170 93 4666
Fragments arranged by size:
4666 1170 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2059 2059
Size: 5929
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2308 2460 2996 3123 3248 3990 4075
Size: 399 518 152 536 127 125 742 85
Cuts at: 4075 5146 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 536 518 399 152 127
125 85
BspEI T'CCGG_A
Cuts at: 5592 5592
Size: 5929
BspHI T'CATG_A
Cuts at: 2685 3818 5548 2685
Size: 1133 1730 3066
Fragments arranged by size:
3066 1730 1133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3284 844
Size: 2440 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3816 1394
Size: 429 1993 3507
Fragments arranged by size:
3507 1993 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2112 3143 4424 4598 65
Size: 1183 864 1031 1281 174 1396
Fragments arranged by size:
1396 1281 1183 1031 864 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4573 4760 1700
Size: 110 2763 187 2869
Fragments arranged by size:
2869 2763 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5929
BssHII G'CGCG_C
Cuts at: 1925 2120 1925
Size: 195 5734
BssSI C'ACGA_G
Cuts at: 3989 5001 3989
Size: 1012 4917
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5929
BstBI TT'CG_AA
Cuts at: 4733 4733
Size: 5929
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5929
BstYI r'GATC_y
Cuts at: 1972 2163 3165 3259 3626 4011 4028 5480 5566
Size: 191 1002 94 367 385 17 1452 86
Cuts at: 5566 5578 5924 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1002 385 367 346 191 94
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 3649
Fragments arranged by size:
3649 1728 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5190
Fragments arranged by size:
5190 683 56
BtsI GCAGTG_nn'
Cuts at: 2267 2541 2870 3181 3445 4239 4259 2267
Size: 274 329 311 264 794 20 3937
Fragments arranged by size:
3937 794 329 311 274 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5929
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5894
DraI TTT'AAA
Cuts at: 3006 3233 3006
Size: 227 5702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4936 809
Size: 507 3620 1802
Fragments arranged by size:
3620 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2590 3376
Size: 54 1425 50 275 18 243 517 786
Cuts at: 3376 4265 5894 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 786 517 275 243 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3376 1537
Size: 275 1564 4090
Fragments arranged by size:
4090 1564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2219 3221 3859 1353
Size: 866 1002 638 3423
Fragments arranged by size:
3423 1002 866 638
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2459 4516 4890 5036 387
Size: 1064 138 870 2057 374 146 1280
Fragments arranged by size:
2057 1280 1064 870 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2800 2966 3008 3075 3992 4589 5376
Size: 393 1168 166 42 67 917 597 787
Cuts at: 5376 5644 1239
Size: 268 1524
Fragments arranged by size:
1524 1168 917 787 597 393 268 166
67 42
EcoO109I rG'GnC_Cy
Cuts at: 2137 2137
Size: 5929
EcoRI G'AATT_C
Cuts at: 3065 3238 3616 3065
Size: 173 378 5378
Fragments arranged by size:
5378 378 173
EcoRV GAT'ATC
Cuts at: 3200 3200
Size: 5929
FspI TGC'GCA
Cuts at: 4435 4435
Size: 5929
HaeII r_GCGC'y
Cuts at: 1678 2374 5076 1678
Size: 696 2702 2531
Fragments arranged by size:
2702 2531 696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2913 2945 3189
Size: 32 135 32 576 32 733 32 244
Cuts at: 3189 3221 4570 4602 4644 4676 1373
Size: 32 1349 32 42 32 2626
Fragments arranged by size:
2626 1349 733 576 244 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3273 3519 669
Size: 988 281 1335 246 3079
Fragments arranged by size:
3079 1335 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KasI G'GCGC_C
Cuts at: 2370 2370
Size: 5929
KpnI G_GTAC'C
Cuts at: 2737 3254 2737
Size: 517 5412
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2622 5043 5227 1305
Size: 1317 2421 184 2007
Fragments arranged by size:
2421 2007 1317 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2592 10
Size: 54 1425 343 243 517 3347
Fragments arranged by size:
3347 1425 517 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3045 3887 4246 4405 518
Size: 648 1287 592 842 359 159 2042
Fragments arranged by size:
2042 1287 842 648 592 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2190 2396 3028 3345 4022 5170 5415
Size: 429 155 206 632 317 677 1148 245
Cuts at: 5415 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 632 429 317 245 206
155
NarI GG'CG_CC
Cuts at: 2371 2371
Size: 5929
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5377
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5929
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 3287 4832 1132
Size: 97 2058 1545 2229
Fragments arranged by size:
2229 2058 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2176 1348
Size: 828 5101
PciI A'CATG_T
Cuts at: 4828 4828
Size: 5929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5929
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 5511 5543 3998
Size: 32 1481 32 4384
Fragments arranged by size:
4384 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2137
Size: 5929
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5929
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PspOMI G'GGCC_C
Cuts at: 3119 3119
Size: 5929
PstI C_TGCA'G
Cuts at: 830 3165 3281 830
Size: 2335 116 3478
Fragments arranged by size:
3478 2335 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5929
PvuII CAG'CTG
Cuts at: 2396 3028 2396
Size: 632 5297
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 4177 5889 4177
Size: 1712 4217
SexAI A'CCwGG_T
Cuts at: 2631 2631
Size: 5929
SfcI C'TryA_G
Cuts at: 826 1045 2391 3161 3277 4412 5093 5284 826
Size: 219 1346 770 116 1135 681 191 1471
Fragments arranged by size:
1471 1346 1135 770 681 219 191 116
SfoI GGC'GCC
Cuts at: 2372 2372
Size: 5929
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5929
SmaI CCC'GGG
Cuts at: 1872 3256 1872
Size: 1384 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4033 4934 5196 820
Size: 197 926 2090 901 262 1553
Fragments arranged by size:
2090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3720 493
Size: 3227 2702
SpeI A'CTAG_T
Cuts at: 152 2599 152
Size: 2447 3482
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SspI AAT'ATT
Cuts at: 5 52 3853 5
Size: 47 3801 2081
Fragments arranged by size:
3801 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5929
StyI C'CwwG_G
Cuts at: 513 1065 3115 3368 513
Size: 552 2050 253 3074
Fragments arranged by size:
3074 2050 552 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3956 3973 4126 4311 4756 325
Size: 1255 2376 17 153 185 445 1498
Fragments arranged by size:
2376 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2353 2508 3037
Size: 274 80 33 51 1443 376 155 529
Cuts at: 3037 4175 5887 96
Size: 1138 1712 138
Fragments arranged by size:
1712 1443 1138 529 376 274 155 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 5889 5921 2394
Size: 32 3463 32 2402
Fragments arranged by size:
3463 2402 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5929
XbaI T'CTAG_A
Cuts at: 3265 3265
Size: 5929
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 3254 1870
Size: 1384 4545
XmnI GAAnn'nnTTC
Cuts at: 4058 4058
Size: 5929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI XcmI