Restriction map for
pFN21AE4383
(Circular) (Six-base) MAPSORT of: pFN21AE4383.seq Check: 342 from: 1 to: 5917
pFN21AE4383 5917 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3727 278
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2190 3260 1937
Size: 253 1070 4594
Fragments arranged by size:
4594 1070 253
Acc65I G'GTAC_C
Cuts at: 3147 3238 3147
Size: 91 5826
AclI AA'CG_TT
Cuts at: 1563 4044 4417 1563
Size: 2481 373 3063
Fragments arranged by size:
3063 2481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2742 3980 5364 5632 1632
Size: 1110 1238 1384 268 1917
Fragments arranged by size:
1917 1384 1238 1110 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5720
AflIII A'CryG_T
Cuts at: 1203 4816 5793 1203
Size: 3613 977 1327
Fragments arranged by size:
3613 1327 977
AgeI A'CCGG_T
Cuts at: 4748 4748
Size: 5917
AhdI GACnn_n'nnGTC
Cuts at: 4646 4646
Size: 5917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5917
AlwNI CAG_nnn'CTG
Cuts at: 2528 3660 5232 2528
Size: 1132 1572 3213
Fragments arranged by size:
3213 1572 1132
ApaLI G'TGCA_C
Cuts at: 2370 3974 5130 2370
Size: 1604 1156 3157
Fragments arranged by size:
3157 1604 1156
ApoI r'AATT_y
Cuts at: 1493 2294 2417 2741 2859 2931 3056 3194 3226
Size: 801 123 324 118 72 125 138 32
Cuts at: 3226 3452 3604 5713 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 801 324 226 152 138 125
123 118 72 32
AseI AT'TA_AT
Cuts at: 160 1032 2265 2762 4471 160
Size: 872 1233 497 1709 1606
Fragments arranged by size:
1709 1606 1233 872 497
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
AvaI C'yCGr_G
Cuts at: 1870 1943 3242 1870
Size: 73 1299 4545
Fragments arranged by size:
4545 1299 73
BamHI G'GATC_C
Cuts at: 2588 3247 3614 2588
Size: 659 367 4891
Fragments arranged by size:
4891 659 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3147 3238 4693 618
Size: 325 217 627 1360 91 1455 1842
Fragments arranged by size:
1842 1455 1360 627 325 217 91
BanII G_rGCy'C
Cuts at: 1391 3236 1391
Size: 1845 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4108 4142 1903
Size: 34 2171 34 3678
Fragments arranged by size:
3678 2171 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3809 5019 3809
Size: 1210 4707
BclI T'GATC_A
Cuts at: 1548 2016 2632 1548
Size: 468 616 4833
Fragments arranged by size:
4833 616 468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4528 136
Size: 107 122 71 631 605 2856 1525
Fragments arranged by size:
2856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5912 5912
Size: 5917
BlpI GC'TnA_GC
Cuts at: 3334 3334
Size: 5917
Bme1580I G_kGCm'C
Cuts at: 1790 2374 3978 5134 1790
Size: 584 1604 1156 2573
Fragments arranged by size:
2573 1604 1156 584
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5917
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4606 442
Size: 416 53 83 399 24 2231 958 1753
Fragments arranged by size:
2231 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2931 4577 1239
Size: 1692 1646 2579
Fragments arranged by size:
2579 1692 1646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4042 4907 5205 4042
Size: 865 298 4754
Fragments arranged by size:
4754 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2981 3108 4580 882
Size: 617 1482 127 1472 2219
Fragments arranged by size:
2219 1482 1472 617 127
BsaAI yAC'GTr
Cuts at: 493 1204 3708 493
Size: 711 2504 2702
Fragments arranged by size:
2702 2504 711
BsaBI GATnn'nnATC
Cuts at: 5911 5911
Size: 5917
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3724 4106 4741 275
Size: 53 83 186 936 2191 382 635 1451
Fragments arranged by size:
2191 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2990 4350 4748 5022 5169 5580 2990
Size: 1360 398 274 147 411 3327
Fragments arranged by size:
3327 1360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5692
Fragments arranged by size:
5692 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2466 2939 3198 1186
Size: 1280 473 259 3905
Fragments arranged by size:
3905 1280 473 259
BseYI C'CCAG_C
Cuts at: 5120 5616 5120
Size: 496 5421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2396 2555 1163
Size: 573 660 159 4525
Fragments arranged by size:
4525 660 573 159
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3367 4128 5156 1540
Size: 275 186 1366 761 1028 2301
Fragments arranged by size:
2301 1366 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2374 3978 4063 5134 2374
Size: 1604 85 1071 3157
Fragments arranged by size:
3157 1604 1071 85
BsmI GAATG_Cn'
Cuts at: 3428 3521 3428
Size: 93 5824
BsmBI CGTCTCn'nnnn_
Cuts at: 3180 3180
Size: 5917
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2374 3236 3978 4063 5134 1391
Size: 399 584 862 742 85 1071 2174
Fragments arranged by size:
2174 1071 862 742 584 399 85
BspEI T'CCGG_A
Cuts at: 5580 5580
Size: 5917
BspHI T'CATG_A
Cuts at: 3806 5536 3806
Size: 1730 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3272 844
Size: 2428 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3804 1394
Size: 429 1981 3507
Fragments arranged by size:
3507 1981 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2905 4412 4586 65
Size: 1183 1657 1507 174 1396
Fragments arranged by size:
1657 1507 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4561 4748 1700
Size: 110 2751 187 2869
Fragments arranged by size:
2869 2751 187 110
BsrGI T'GTAC_A
Cuts at: 96 2137 96
Size: 2041 3876
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5917
BssSI C'ACGA_G
Cuts at: 2043 2097 3977 4989 2043
Size: 54 1880 1012 2971
Fragments arranged by size:
2971 1880 1012 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2528 794
Size: 1734 4183
BstBI TT'CG_AA
Cuts at: 4721 4721
Size: 5917
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5917
BstYI r'GATC_y
Cuts at: 1972 2428 2588 3247 3614 3999 4016 5468 5554
Size: 456 160 659 367 385 17 1452 86
Cuts at: 5554 5566 5912 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 659 456 385 367 346 160
86 17 12
BstZ17I GTA'TAC
Cuts at: 2191 2191
Size: 5917
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5365
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5178
Fragments arranged by size:
5178 683 56
BtsI GCAGTG_nn'
Cuts at: 3433 4227 4247 3433
Size: 794 20 5103
Fragments arranged by size:
5103 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5917
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5882
DraI TTT'AAA
Cuts at: 2602 2658 3221 2602
Size: 56 563 5298
Fragments arranged by size:
5298 563 56
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4924 809
Size: 507 3608 1802
Fragments arranged by size:
3608 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3364 4253 5882
Size: 54 1425 50 275 18 1534 889 1629
Cuts at: 5882 8
Size: 43
Fragments arranged by size:
1629 1534 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3364 1537
Size: 275 1552 4090
Fragments arranged by size:
4090 1552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2916 3847 1353
Size: 1563 931 3423
Fragments arranged by size:
3423 1563 931
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4504 4878 5024 387
Size: 1064 138 2915 374 146 1280
Fragments arranged by size:
2915 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2742 2931 3980 4577 5364 5632 1239
Size: 393 1110 189 1049 597 787 268 1524
Fragments arranged by size:
1524 1110 1049 787 597 393 268 189
EcoRI G'AATT_C
Cuts at: 3226 3604 3226
Size: 378 5539
EcoRV GAT'ATC
Cuts at: 2010 2010
Size: 5917
FspI TGC'GCA
Cuts at: 4423 4423
Size: 5917
HaeII r_GCGC'y
Cuts at: 1678 5064 1678
Size: 3386 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 4558 4590 4632
Size: 32 135 32 436 32 2518 32 42
Cuts at: 4632 4664 1373
Size: 32 2626
Fragments arranged by size:
2626 2518 436 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 3261 3507 669
Size: 988 281 298 1025 246 3079
Fragments arranged by size:
3079 1025 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5917
HpaI GTT'AAC
Cuts at: 3507 3507
Size: 5917
KpnI G_GTAC'C
Cuts at: 3151 3242 3151
Size: 91 5826
MfeI C'AATT_G
Cuts at: 2202 3516 2202
Size: 1314 4603
MluI A'CGCG_T
Cuts at: 5793 5793
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2757 3009 3051 5031 5215 1305
Size: 1452 252 42 1980 184 2007
Fragments arranged by size:
2007 1980 1452 252 184 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4095
Fragments arranged by size:
4095 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2505 3875 4234 4393 518
Size: 648 1339 1370 359 159 2042
Fragments arranged by size:
2042 1370 1339 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2539 2594 3333 4010 5158 5403 1606
Size: 933 55 739 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 739 677 245 55
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5365
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5917
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5917
NotI GC'GGCC_GC
Cuts at: 3364 3364
Size: 5917
NspI r_CATG'y
Cuts at: 1132 1229 3022 3275 4820 1132
Size: 97 1793 253 1545 2229
Fragments arranged by size:
2229 1793 1545 253 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5917
PciI A'CATG_T
Cuts at: 4816 4816
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3169 1108
Size: 2061 3856
PmeI GTTT'AAAC
Cuts at: 3221 3221
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 5499 5531 3986
Size: 32 1481 32 4372
Fragments arranged by size:
4372 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5917
PsiI TTA'TAA
Cuts at: 2160 3487 2160
Size: 1327 4590
PstI C_TGCA'G
Cuts at: 830 3269 830
Size: 2439 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5917
PvuII CAG'CTG
Cuts at: 2539 2539
Size: 5917
SalI G'TCGA_C
Cuts at: 1936 3259 1936
Size: 1323 4594
SbfI CC_TGCA'GG
Cuts at: 3269 3269
Size: 5917
ScaI AGT'ACT
Cuts at: 2807 4165 5877 2807
Size: 1358 1712 2847
Fragments arranged by size:
2847 1712 1358
SfcI C'TryA_G
Cuts at: 826 1045 2853 3265 4400 5081 5272 826
Size: 219 1808 412 1135 681 191 1471
Fragments arranged by size:
1808 1471 1135 681 412 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5917
SmaI CCC'GGG
Cuts at: 1872 3244 1872
Size: 1372 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4021 4922 5184 820
Size: 197 926 2078 901 262 1553
Fragments arranged by size:
2078 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3708 493
Size: 3215 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5917
SphI G_CATG'C
Cuts at: 1132 3022 3275 1132
Size: 1890 253 3774
Fragments arranged by size:
3774 1890 253
SspI AAT'ATT
Cuts at: 5 52 3841 5
Size: 47 3789 2081
Fragments arranged by size:
3789 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5917
StyI C'CwwG_G
Cuts at: 513 1065 2389 2911 3178 3356 513
Size: 552 1324 522 267 178 3074
Fragments arranged by size:
3074 1324 552 522 267 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3944 3961 4114 4299 4744 325
Size: 1255 2364 17 153 185 445 1498
Fragments arranged by size:
2364 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2137 2805 4163
Size: 274 80 33 51 1443 160 668 1358
Cuts at: 4163 5875 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1358 668 274 160 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5877 5909 5877
Size: 32 5885
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5917
XbaI T'CTAG_A
Cuts at: 3253 3253
Size: 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5917
XmaI C'CCGG_G
Cuts at: 1870 3242 1870
Size: 1372 4545
XmnI GAAnn'nnTTC
Cuts at: 4046 4046
Size: 5917
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3725 276
Size: 53 83 186 936 2191 2468
Fragments arranged by size:
2468 2191 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI