Restriction map for
pFN21AE4378
(Circular) (Six-base) MAPSORT of: pFN21AE4378.seq Check: 3463 from: 1 to: 5902
pFN21AE4378 5902 bp
With 182 enzymes: SgfI *
August 16, 2021 15:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2130 3245 1937
Size: 193 1115 4594
Fragments arranged by size:
4594 1115 193
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2716 2860 3965 5349 5617 1632
Size: 1084 144 1105 1384 268 1917
Fragments arranged by size:
1917 1384 1105 1084 268 144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 2477 4801 5778 1203
Size: 1274 2324 977 1327
Fragments arranged by size:
2324 1327 1274 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 2397 3645 5217 2397
Size: 1248 1572 3082
Fragments arranged by size:
3082 1572 1248
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 3211 3437 3589 5698 1493
Size: 1718 226 152 2109 1697
Fragments arranged by size:
2109 1718 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2176 3227 1870
Size: 73 233 1051 4545
Fragments arranged by size:
4545 1051 233 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2496 3223 4678 618
Size: 325 217 627 709 727 1455 1842
Fragments arranged by size:
1842 1455 727 709 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3221 1391
Size: 1830 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2269 2394 2480 2654 2683 928
Size: 971 370 125 86 174 29 4147
Fragments arranged by size:
4147 971 370 174 125 86 29
BbvCI CC'TCA_GC
Cuts at: 3117 3117
Size: 5902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2755 2789 4093 4127 1903
Size: 34 818 34 1304 34 3678
Fragments arranged by size:
3678 1304 818 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3082 4513 136
Size: 107 122 71 631 605 1410 1431 1525
Fragments arranged by size:
1525 1431 1410 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2484 5897 2484
Size: 3413 2489
BlpI GC'TnA_GC
Cuts at: 3141 3319 3141
Size: 178 5724
Bme1580I G_kGCm'C
Cuts at: 1790 2501 2687 3963 5119 1790
Size: 711 186 1276 1156 2573
Fragments arranged by size:
2573 1276 1156 711 186
BmgBI CAC'GTC
Cuts at: 1318 2478 1318
Size: 1160 4742
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2681 3633 4591
Size: 416 53 83 399 24 1264 952 958
Cuts at: 4591 442
Size: 1753
Fragments arranged by size:
1753 1264 958 952 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4562 1239
Size: 3323 2579
Bpu10I CC'TnA_GC
Cuts at: 3117 3117
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2328 4027 4892 5190 2195
Size: 133 1699 865 298 2907
Fragments arranged by size:
2907 1699 865 298 133
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2707 2803 3693 493
Size: 711 1503 96 890 2702
Fragments arranged by size:
2702 1503 890 711 96
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3709 4091 4726 275
Size: 53 83 186 936 2176 382 635 1451
Fragments arranged by size:
2176 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2639 1186
Size: 853 600 4449
Fragments arranged by size:
4449 853 600
BseYI C'CCAG_C
Cuts at: 2229 2281 5105 5601 2229
Size: 52 2824 496 2530
Fragments arranged by size:
2824 2530 496 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2657 1163
Size: 573 921 4408
Fragments arranged by size:
4408 921 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2364 3963 4048 5119 2364
Size: 1599 85 1071 3147
Fragments arranged by size:
3147 1599 1071 85
BsiWI C'GTAC_G
Cuts at: 2803 2803
Size: 5902
BsmI GAATG_Cn'
Cuts at: 2638 3413 3506 2638
Size: 775 93 5034
Fragments arranged by size:
5034 775 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2501 2687 3221 3963 4048 5119
Size: 399 574 137 186 534 742 85 1071
Cuts at: 5119 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 574 534 399 186 137
85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 2310 3791 5521 2310
Size: 1481 1730 2691
Fragments arranged by size:
2691 1730 1481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2660 3063 3257 844
Size: 1816 403 194 3489
Fragments arranged by size:
3489 1816 403 194
BsrBI CCG'CTC
Cuts at: 1394 1823 2181 3789 1394
Size: 429 358 1608 3507
Fragments arranged by size:
3507 1608 429 358
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstXI CCAn_nnnn'nTGG
Cuts at: 2237 2237
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2484 3232 3599 3984 4001 5453 5539 5551
Size: 512 748 367 385 17 1452 86 12
Cuts at: 5551 5897 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 748 512 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2502 2833 513
Size: 552 939 498 331 3582
Fragments arranged by size:
3582 939 552 498 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2200 2809 3418 4212 4232 2200
Size: 609 609 794 20 3870
Fragments arranged by size:
3870 794 609 609 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DraIII CAC_nnn'GTG
Cuts at: 2896 2896
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2220 3036
Size: 54 1425 50 275 18 201 189 816
Cuts at: 3036 3349 4238 5867 8
Size: 313 889 1629 43
Fragments arranged by size:
1629 1425 889 816 313 275 201 189
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 2841 2930 3832 1353
Size: 695 793 89 902 3423
Fragments arranged by size:
3423 902 793 695 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2716 2860 3965 4562 5349 5617 1239
Size: 393 1084 144 1105 597 787 268 1524
Fragments arranged by size:
1524 1105 1084 787 597 393 268 144
EcoNI CCTnn'n_nnAGG
Cuts at: 2621 2927 2621
Size: 306 5596
EcoO109I rG'GnC_Cy
Cuts at: 2215 2215
Size: 5902
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FspI TGC'GCA
Cuts at: 2356 4408 2356
Size: 2052 3850
HaeII r_GCGC'y
Cuts at: 1678 5049 1678
Size: 3371 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4543 4575 4617 4649 1373
Size: 32 135 32 2971 32 42 32 2626
Fragments arranged by size:
2971 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2605 3246 3492 669
Size: 988 281 667 641 246 3079
Fragments arranged by size:
3079 988 667 641 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2128 5016 5200 1305
Size: 823 2888 184 2007
Fragments arranged by size:
2888 2007 823 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3038 10
Size: 54 1425 343 1206 2874
Fragments arranged by size:
2874 1425 1206 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2957 3860 4219 4378 518
Size: 648 1791 903 359 159 2042
Fragments arranged by size:
2042 1791 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2116 3318 3995 5143 5388 1606
Size: 510 1202 677 1148 245 2120
Fragments arranged by size:
2120 1202 1148 677 510 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2833 513
Size: 552 939 829 3582
Fragments arranged by size:
3582 939 829 552
NdeI CA'TA_TG
Cuts at: 387 2378 387
Size: 1991 3911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2287 2405 3172 1348
Size: 939 118 767 4078
Fragments arranged by size:
4078 939 767 118
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2119 2778 1108
Size: 1011 659 4232
Fragments arranged by size:
4232 1011 659
PfoI T'CCnGG_A
Cuts at: 2019 2019
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PspXI vC'TCGA_Gb
Cuts at: 2176 2176
Size: 5902
PstI C_TGCA'G
Cuts at: 830 2816 3183 3254 830
Size: 1986 367 71 3478
Fragments arranged by size:
3478 1986 367 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2116 2116
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3183 3254 3183
Size: 71 5831
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SexAI A'CCwGG_T
Cuts at: 2616 2616
Size: 5902
SfcI C'TryA_G
Cuts at: 826 1045 2812 3179 3250 4385 5066 5257 826
Size: 219 1767 367 71 1135 681 191 1471
Fragments arranged by size:
1767 1471 1135 681 367 219 191 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2176 2210 2343 4006 4907 5169
Size: 197 926 233 34 133 1663 901 262
Cuts at: 5169 820
Size: 1553
Fragments arranged by size:
1663 1553 926 901 262 233 197 133
34
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 2004 2428 2833 3341 513
Size: 552 939 424 405 508 3074
Fragments arranged by size:
3074 939 552 508 424 405
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2134 2379 3929 3946 4099 4284 4729
Size: 1255 554 245 1550 17 153 185 445
Cuts at: 4729 325
Size: 1498
Fragments arranged by size:
1550 1498 1255 554 445 245 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 5862 5894 2001
Size: 32 3829 32 2009
Fragments arranged by size:
3829 2009 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2273 1316
Size: 957 4945
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2893 3169 2893
Size: 276 5626
XhoI C'TCGA_G
Cuts at: 1943 2176 1943
Size: 233 5669
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 3104 4031 3104
Size: 927 4975
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI