Restriction map for
pFN21AE4375
(Circular) (Six-base) MAPSORT of: pFN21AE4375.seq Check: 2997 from: 1 to: 5890
pFN21AE4375 5890 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2742 2742
Size: 5890
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3700 278
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 3233 1937
Size: 790 506 4594
Fragments arranged by size:
4594 790 506
Acc65I G'GTAC_C
Cuts at: 3211 3211
Size: 5890
AclI AA'CG_TT
Cuts at: 1563 4017 4390 1563
Size: 2454 373 3063
Fragments arranged by size:
3063 2454 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2690 3953 5337 5605 1632
Size: 1058 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 1058 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5693
AflIII A'CryG_T
Cuts at: 1203 4789 5766 1203
Size: 3586 977 1327
Fragments arranged by size:
3586 1327 977
AgeI A'CCGG_T
Cuts at: 2014 4721 2014
Size: 2707 3183
AhdI GACnn_n'nnGTC
Cuts at: 4619 4619
Size: 5890
AleI CACnn'nnGTG
Cuts at: 1166 2870 1166
Size: 1704 4186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 2950 1184
Size: 1032 734 4124
Fragments arranged by size:
4124 1032 734
AlwNI CAG_nnn'CTG
Cuts at: 3633 5205 3633
Size: 1572 4318
ApaI G_GGCC'C
Cuts at: 2668 2668
Size: 5890
ApaLI G'TGCA_C
Cuts at: 3947 5103 3947
Size: 1156 4734
ApoI r'AATT_y
Cuts at: 1493 3199 3425 3577 5686 1493
Size: 1706 226 152 2109 1697
Fragments arranged by size:
2109 1706 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4444 160
Size: 872 3412 1606
Fragments arranged by size:
3412 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
AvaI C'yCGr_G
Cuts at: 1870 1943 3215 1870
Size: 73 1272 4545
Fragments arranged by size:
4545 1272 73
BamHI G'GATC_C
Cuts at: 3220 3587 3220
Size: 367 5523
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2695 3211 4666 618
Size: 325 217 627 908 516 1455 1842
Fragments arranged by size:
1842 1455 908 627 516 325 217
BanII G_rGCy'C
Cuts at: 1391 2668 2833 2934 3209 1391
Size: 1277 165 101 275 4072
Fragments arranged by size:
4072 1277 275 165 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2047 2643 2724 3029 3128 928
Size: 971 148 596 81 305 99 3690
Fragments arranged by size:
3690 971 596 305 148 99 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4081 4115 1903
Size: 34 2144 34 3678
Fragments arranged by size:
3678 2144 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4992 3782
Size: 1210 4680
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5890
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2165 2495 2746
Size: 107 122 71 631 605 493 330 251
Cuts at: 2746 4501 136
Size: 1755 1525
Fragments arranged by size:
1755 1525 631 605 493 330 251 122
107 71
BglII A'GATC_T
Cuts at: 2100 5885 2100
Size: 3785 2105
BlpI GC'TnA_GC
Cuts at: 2881 3307 2881
Size: 426 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2668 3951 5107 1790
Size: 878 1283 1156 2573
Fragments arranged by size:
2573 1283 1156 878
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2353 2736 3621
Size: 416 53 83 399 24 936 383 885
Cuts at: 3621 4579 442
Size: 958 1753
Fragments arranged by size:
1753 958 936 885 416 399 383 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5890
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 5890
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 4550 1239
Size: 1430 1881 2579
Fragments arranged by size:
2579 1881 1430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 4015 4880 5178 2126
Size: 1889 865 298 2838
Fragments arranged by size:
2838 1889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4553 882
Size: 617 3054 2219
Fragments arranged by size:
3054 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3681 493
Size: 711 2477 2702
Fragments arranged by size:
2702 2477 711
BsaBI GATnn'nnATC
Cuts at: 2136 5884 2136
Size: 3748 2142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3078 3697 4079 4714
Size: 53 83 186 936 1545 619 382 635
Cuts at: 4714 275
Size: 1451
Fragments arranged by size:
1545 1451 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2014 4323 4721 4995 5142 5553 2014
Size: 2309 398 274 147 411 2351
Fragments arranged by size:
2351 2309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5665
Fragments arranged by size:
5665 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2459 2510 2666 1186
Size: 1213 60 51 156 4410
Fragments arranged by size:
4410 1213 156 60 51
BseYI C'CCAG_C
Cuts at: 2823 2901 5093 5589 2823
Size: 78 2192 496 3124
Fragments arranged by size:
3124 2192 496 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2645 1163
Size: 573 909 4408
Fragments arranged by size:
4408 909 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3074 3340 4101 5129 1540
Size: 275 186 1073 266 761 1028 2301
Fragments arranged by size:
2301 1073 1028 761 275 266 186
BsiHKAI G_wGCw'C
Cuts at: 3951 4036 5107 3951
Size: 85 1071 4734
Fragments arranged by size:
4734 1071 85
BsmI GAATG_Cn'
Cuts at: 3401 3494 3401
Size: 93 5797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2668 2833 2934 3209 3951 4036 5107
Size: 399 878 165 101 275 742 85 1071
Cuts at: 5107 1391
Size: 2174
Fragments arranged by size:
2174 1071 878 742 399 275 165 101
85
BspEI T'CCGG_A
Cuts at: 5553 5553
Size: 5890
BspHI T'CATG_A
Cuts at: 3779 5509 3779
Size: 1730 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2742 3245 844
Size: 1898 503 3489
Fragments arranged by size:
3489 1898 503
BsrBI CCG'CTC
Cuts at: 1394 1823 2880 3777 1394
Size: 429 1057 897 3507
Fragments arranged by size:
3507 1057 897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4385 4559 65
Size: 1183 3137 174 1396
Fragments arranged by size:
3137 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2189 2698 4534 4721 1700
Size: 110 204 175 509 1836 187 2869
Fragments arranged by size:
2869 1836 509 204 187 175 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5890
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5890
BssSI C'ACGA_G
Cuts at: 3950 4962 3950
Size: 1012 4878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5890
BstBI TT'CG_AA
Cuts at: 4694 4694
Size: 5890
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 2917 2974 2710
Size: 207 57 5626
Fragments arranged by size:
5626 207 57
BstYI r'GATC_y
Cuts at: 1972 2100 3220 3587 3972 3989 5441 5527 5539
Size: 128 1120 367 385 17 1452 86 12
Cuts at: 5539 5885 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1120 385 367 346 128 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2504 2504
Size: 5890
BtgI C'CryG_G
Cuts at: 513 1065 2229 2691 3161 513
Size: 552 1164 462 470 3242
Fragments arranged by size:
3242 1164 552 470 462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5151
Fragments arranged by size:
5151 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 3406 4200 4220 2130
Size: 1276 794 20 3800
Fragments arranged by size:
3800 1276 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5890
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5855
DraI TTT'AAA
Cuts at: 3194 3194
Size: 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4897 809
Size: 507 3581 1802
Fragments arranged by size:
3581 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2700 2791
Size: 54 1425 50 275 18 396 474 91
Cuts at: 2791 2907 2976 3071 3136 3337 4226 5855 8
Size: 116 69 95 65 201 889 1629 43
Fragments arranged by size:
1629 1425 889 474 396 275 201 116
95 91 69 65 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3071 3337 1537
Size: 275 1259 266 4090
Fragments arranged by size:
4090 1259 275 266
EarI CTCTTCn'nnn_
Cuts at: 1353 3820 1353
Size: 2467 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4477 4851 4997 387
Size: 1064 138 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 2690 3953 4550 5337 5605 1239
Size: 393 1037 21 1263 597 787 268 1524
Fragments arranged by size:
1524 1263 1037 787 597 393 268 21
EcoO109I rG'GnC_Cy
Cuts at: 2664 2665 2765 2844 2664
Size: 1 100 79 5710
Fragments arranged by size:
5710 100 79 1
EcoRI G'AATT_C
Cuts at: 3199 3577 3199
Size: 378 5512
FspI TGC'GCA
Cuts at: 2600 2660 4396 2600
Size: 60 1736 4094
Fragments arranged by size:
4094 1736 60
FspAI rTGC'GCAy
Cuts at: 2600 2600
Size: 5890
HaeII r_GCGC'y
Cuts at: 1678 2512 5037 1678
Size: 834 2525 2531
Fragments arranged by size:
2531 2525 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 2934 2966 2972
Size: 32 135 32 755 32 575 32 6
Cuts at: 2972 3004 4531 4563 4605 4637 1373
Size: 32 1527 32 42 32 2626
Fragments arranged by size:
2626 1527 755 575 135 42 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3480 669
Size: 988 281 1296 246 3079
Fragments arranged by size:
3079 1296 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3179 748
Size: 2431 3459
HpaI GTT'AAC
Cuts at: 3480 3480
Size: 5890
KpnI G_GTAC'C
Cuts at: 3215 3215
Size: 5890
MfeI C'AATT_G
Cuts at: 3489 3489
Size: 5890
MluI A'CGCG_T
Cuts at: 5766 5766
Size: 5890
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5004 5188 1305
Size: 3699 184 2007
Fragments arranged by size:
3699 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2228 2909 10
Size: 54 1425 343 396 681 2991
Fragments arranged by size:
2991 1425 681 396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2594 2621 2838 2870 2972 3848
Size: 648 1056 372 27 217 32 102 876
Cuts at: 3848 4207 4366 518
Size: 359 159 2042
Fragments arranged by size:
2042 1056 876 648 372 359 217 159
102 32 27
MspA1I CmG'CkG
Cuts at: 1606 2245 2901 2986 3306 3983 5131 5376 1606
Size: 639 656 85 320 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 656 639 320 245 85
NaeI GCC'GGC
Cuts at: 2700 2700
Size: 5890
NcoI C'CATG_G
Cuts at: 513 1065 2229 3161 513
Size: 552 1164 932 3242
Fragments arranged by size:
3242 1164 932 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5890
NgoMIV G'CCGG_C
Cuts at: 2698 2698
Size: 5890
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5890
NotI GC'GGCC_GC
Cuts at: 3337 3337
Size: 5890
NspI r_CATG'y
Cuts at: 1132 1229 2455 2572 3132 3248 4793 1132
Size: 97 1226 117 560 116 1545 2229
Fragments arranged by size:
2229 1545 1226 560 117 116 97
PasI CC'CwG_GG
Cuts at: 1348 2770 1348
Size: 1422 4468
PciI A'CATG_T
Cuts at: 4789 4789
Size: 5890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 1108
Size: 1140 4750
PmeI GTTT'AAAC
Cuts at: 3194 3194
Size: 5890
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 5472 5504 3959
Size: 32 1481 32 4345
Fragments arranged by size:
4345 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2765 2765
Size: 5890
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5890
PsiI TTA'TAA
Cuts at: 3460 3460
Size: 5890
PspOMI G'GGCC_C
Cuts at: 2664 2664
Size: 5890
PstI C_TGCA'G
Cuts at: 830 2739 3242 830
Size: 1909 503 3478
Fragments arranged by size:
3478 1909 503
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5890
SalI G'TCGA_C
Cuts at: 1936 3232 1936
Size: 1296 4594
SbfI CC_TGCA'GG
Cuts at: 3242 3242
Size: 5890
ScaI AGT'ACT
Cuts at: 4138 5850 4138
Size: 1712 4178
SfcI C'TryA_G
Cuts at: 826 1045 2735 3238 4373 5054 5245 826
Size: 219 1690 503 1135 681 191 1471
Fragments arranged by size:
1690 1471 1135 681 503 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5890
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5890
SmaI CCC'GGG
Cuts at: 1872 3217 1872
Size: 1345 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 3994 4895 5157 820
Size: 197 926 198 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 198 197
SnaBI TAC'GTA
Cuts at: 493 3681 493
Size: 3188 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5890
SphI G_CATG'C
Cuts at: 1132 2455 3132 3248 1132
Size: 1323 677 116 3774
Fragments arranged by size:
3774 1323 677 116
SspI AAT'ATT
Cuts at: 5 52 3814 5
Size: 47 3762 2081
Fragments arranged by size:
3762 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5890
StyI C'CwwG_G
Cuts at: 513 1065 2160 2229 2292 2868 3161 3329 513
Size: 552 1095 69 63 576 293 168 3074
Fragments arranged by size:
3074 1095 576 552 293 168 69 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 3917 3934 4087 4272 4717 325
Size: 1255 1313 1024 17 153 185 445 1498
Fragments arranged by size:
1498 1313 1255 1024 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2565 4136 5848
Size: 274 80 33 51 1443 588 1571 1712
Cuts at: 5848 96
Size: 138
Fragments arranged by size:
1712 1571 1443 588 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 5850 5882 2012
Size: 32 3806 32 2020
Fragments arranged by size:
3806 2020 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2723 1316
Size: 1407 4483
XbaI T'CTAG_A
Cuts at: 3226 3226
Size: 5890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2161
Size: 5890
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5890
XmaI C'CCGG_G
Cuts at: 1870 3215 1870
Size: 1345 4545
XmnI GAAnn'nnTTC
Cuts at: 4019 4019
Size: 5890
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3698 276
Size: 53 83 186 936 2164 2468
Fragments arranged by size:
2468 2164 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI