Restriction map for
pFN21AE4373
(Circular) (Six-base) MAPSORT of: pFN21AE4373.seq Check: 9538 from: 1 to: 5878
pFN21AE4373 5878 bp
With 182 enzymes: SgfI *
March 28, 2012 13:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2281 3221 1937
Size: 344 940 4594
Fragments arranged by size:
4594 940 344
Acc65I G'GTAC_C
Cuts at: 3072 3199 3072
Size: 127 5751
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 3941 5325 5593 1632
Size: 858 1451 1384 268 1917
Fragments arranged by size:
1917 1451 1384 858 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 4777 5754 1203
Size: 3574 977 1327
Fragments arranged by size:
3574 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 3621 5193 3621
Size: 1572 4306
ApaI G_GGCC'C
Cuts at: 2904 2904
Size: 5878
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2413 3187 3413 3565 5674 1493
Size: 920 774 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 774 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 3203 1870
Size: 73 1260 4545
Fragments arranged by size:
4545 1260 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3072 3199 4654 618
Size: 325 217 627 1285 127 1455 1842
Fragments arranged by size:
1842 1455 1285 627 325 217 127
BanII G_rGCy'C
Cuts at: 1391 2548 2904 3197 1391
Size: 1157 356 293 4072
Fragments arranged by size:
4072 1157 356 293
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2495 5873 2495
Size: 3378 2500
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 2904 3939 5095 1790
Size: 1114 1035 1156 2573
Fragments arranged by size:
2573 1156 1114 1035
BmgBI CAC'GTC
Cuts at: 1318 2285 1318
Size: 967 4911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 3609 4567
Size: 416 53 83 399 24 1024 1168 958
Cuts at: 4567 442
Size: 1753
Fragments arranged by size:
1753 1168 1024 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4538 1239
Size: 3299 2579
Bpu10I CC'TnA_GC
Cuts at: 2190 2190
Size: 5878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2971 2995 4003 4868 5166 2971
Size: 24 1008 865 298 3683
Fragments arranged by size:
3683 1008 865 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2558 2759 2879 2954 3086 3669 493
Size: 711 1354 201 120 75 132 583 2702
Fragments arranged by size:
2702 1354 711 583 201 132 120 75
BsaBI GATnn'nnATC
Cuts at: 2766 5872 2766
Size: 3106 2772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5653
Fragments arranged by size:
5653 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2358 2475 3069 1186
Size: 1172 117 594 3995
Fragments arranged by size:
3995 1172 594 117
BseYI C'CCAG_C
Cuts at: 2179 5081 5577 2179
Size: 2902 496 2480
Fragments arranged by size:
2902 2480 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2484 1163
Size: 573 748 4557
Fragments arranged by size:
4557 748 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2548 2887 3118 3939 4024 5095 2548
Size: 339 231 821 85 1071 3331
Fragments arranged by size:
3331 1071 821 339 231 85
BsmI GAATG_Cn'
Cuts at: 3389 3482 3389
Size: 93 5785
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2548 2887 2904 3118 3197 3939 4024
Size: 399 758 339 17 214 79 742 85
Cuts at: 4024 5095 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 758 742 399 339 214 85
79 17
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3233 844
Size: 2389 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4373 4547 65
Size: 1183 3125 174 1396
Fragments arranged by size:
3125 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 2688 2718 2895 3938 4950 2688
Size: 30 177 1043 1012 3616
Fragments arranged by size:
3616 1043 1012 177 30
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2245
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2495 3208 3575 3960 3977 5429 5515 5527
Size: 523 713 367 385 17 1452 86 12
Cuts at: 5527 5873 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 523 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3109 3133 513
Size: 552 939 1105 24 3258
Fragments arranged by size:
3258 1105 939 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 2533 3394 4188 4208 2533
Size: 861 794 20 4203
Fragments arranged by size:
4203 861 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DraIII CAC_nnn'GTG
Cuts at: 2882 2882
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3130 3325 4214
Size: 54 1425 50 275 18 1300 195 889
Cuts at: 4214 5843 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1300 889 275 195 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2335 2536 2557 3808 1353
Size: 686 296 201 21 1251 3423
Fragments arranged by size:
3423 1251 686 296 201 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2546 2546
Size: 5878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2490 3941 4538 5325 5593 1239
Size: 393 858 1451 597 787 268 1524
Fragments arranged by size:
1524 1451 858 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2322 2895 2322
Size: 573 5305
EcoO109I rG'GnC_Cy
Cuts at: 2315 2366 2480 2900 2901 2315
Size: 51 114 420 1 5292
Fragments arranged by size:
5292 420 114 51 1
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 5025 1678
Size: 3347 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2756 2788 2969 2993 3001
Size: 32 135 32 1184 32 181 24 8
Cuts at: 3001 3025 4519 4551 4593 4625 1373
Size: 24 1494 32 42 32 2626
Fragments arranged by size:
2626 1494 1184 181 135 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3076 3203 3076
Size: 127 5751
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4992 5176 1305
Size: 3687 184 2007
Fragments arranged by size:
3687 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4056
Fragments arranged by size:
4056 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2391 2832 3836 4195 4354 518
Size: 648 1077 148 441 1004 359 159 2042
Fragments arranged by size:
2042 1077 1004 648 441 359 159 148
MspA1I CmG'CkG
Cuts at: 1606 2599 3111 3294 3971 5119 5364 1606
Size: 993 512 183 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 677 512 245 183
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4387
Fragments arranged by size:
4387 939 552
NdeI CA'TA_TG
Cuts at: 387 2857 387
Size: 2470 3408
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 3236 4781 1132
Size: 97 2007 1545 2229
Fragments arranged by size:
2229 2007 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2474 2915 3125 1108
Size: 1366 441 210 3861
Fragments arranged by size:
3861 1366 441 210
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PmlI CAC'GTG
Cuts at: 2558 2759 2954 3086 2558
Size: 201 195 132 5350
Fragments arranged by size:
5350 201 195 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2366 2480 2366
Size: 114 5764
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PspOMI G'GGCC_C
Cuts at: 2900 2900
Size: 5878
PstI C_TGCA'G
Cuts at: 830 3230 830
Size: 2400 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
SacI G_AGCT'C
Cuts at: 2548 2548
Size: 5878
SacII CC_GC'GG
Cuts at: 3112 3112
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SapI GCTCTTCn'nnn_
Cuts at: 2039 2536 2039
Size: 497 5381
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 2115 4126 5838 2115
Size: 2011 1712 2155
Fragments arranged by size:
2155 2011 1712
SfcI C'TryA_G
Cuts at: 826 1045 2845 3094 3226 4361 5042 5233 826
Size: 219 1800 249 132 1135 681 191 1471
Fragments arranged by size:
1800 1471 1135 681 249 219 191 132
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2986 3010 3982 4883 5145 820
Size: 197 926 1043 24 972 901 262 1553
Fragments arranged by size:
1553 1043 972 926 901 262 197 24
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2004 3317 513
Size: 552 939 1313 3074
Fragments arranged by size:
3074 1313 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3905 3922 4075 4260 4705 325
Size: 1255 2325 17 153 185 445 1498
Fragments arranged by size:
2325 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2113 4124 5836
Size: 274 80 33 51 1443 136 2011 1712
Cuts at: 5836 96
Size: 138
Fragments arranged by size:
2011 1712 1443 274 138 136 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 5838 5870 2091
Size: 32 3715 32 2099
Fragments arranged by size:
3715 2099 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 4007 4007
Size: 5878
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI PvuII RsrII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI