Restriction map for
pFN21AE4370
(Circular) (Six-base) MAPSORT of: pFN21AE4370.seq Check: 6001 from: 1 to: 5866
pFN21AE4370 5866 bp
With 182 enzymes: SgfI *
March 28, 2012 14:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2210 3209 1937
Size: 273 999 4594
Fragments arranged by size:
4594 999 273
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3929 5313 5581 1632
Size: 2297 1384 268 1917
Fragments arranged by size:
2297 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 4595 4595
Size: 5866
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2053 1184
Size: 869 4997
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2374 2406 2374
Size: 32 5834
AlwNI CAG_nnn'CTG
Cuts at: 2366 3609 5181 2366
Size: 1243 1572 3051
Fragments arranged by size:
3051 1572 1243
ApaI G_GGCC'C
Cuts at: 2677 2677
Size: 5866
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 2917 3175 3401 3553 5662 1493
Size: 1424 258 226 152 2109 1697
Fragments arranged by size:
2109 1697 1424 258 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 3191 1870
Size: 73 1248 4545
Fragments arranged by size:
4545 1248 73
BamHI G'GATC_C
Cuts at: 3196 3563 3196
Size: 367 5499
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2816 3187 4642 618
Size: 325 217 627 1029 371 1455 1842
Fragments arranged by size:
1842 1455 1029 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2677 3005 3133 3185 1391
Size: 705 581 328 128 52 4072
Fragments arranged by size:
4072 705 581 328 128 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2889 928
Size: 971 990 3905
Fragments arranged by size:
3905 990 971
BbvCI CC'TCA_GC
Cuts at: 2367 2871 2367
Size: 504 5362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2367 2401 2413 2447 4057 4091 1903
Size: 34 430 34 12 34 1610 34 3678
Fragments arranged by size:
3678 1610 430 34 34 34 34 12
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4968 3758
Size: 1210 4656
BclI T'GATC_A
Cuts at: 1548 2496 2931 1548
Size: 948 435 4483
Fragments arranged by size:
4483 948 435
BfrBI ATG'CAT
Cuts at: 2568 2568
Size: 5866
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2130 4477 136
Size: 107 122 71 631 605 458 2347 1525
Fragments arranged by size:
2347 1525 631 605 458 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 2677 3927 5083 1790
Size: 887 1250 1156 2573
Fragments arranged by size:
2573 1250 1156 887
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2768 2811 2861 4526 1239
Size: 1529 43 50 1665 2579
Fragments arranged by size:
2579 1665 1529 50 43
Bpu10I CC'TnA_GC
Cuts at: 2095 2367 2871 2095
Size: 272 504 5090
Fragments arranged by size:
5090 504 272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4529 882
Size: 617 3030 2219
Fragments arranged by size:
3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 5860 5860
Size: 5866
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2962 4299 4697 4971 5118 5529 2962
Size: 1337 398 274 147 411 3299
Fragments arranged by size:
3299 1337 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2374 2404 1178
Size: 30 165 30 971 30 4640
Fragments arranged by size:
4640 971 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2682 1186
Size: 1496 4370
BseYI C'CCAG_C
Cuts at: 2317 2507 5069 5565 2317
Size: 190 2562 496 2618
Fragments arranged by size:
2618 2562 496 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2160 2453 1163
Size: 573 424 293 4576
Fragments arranged by size:
4576 573 424 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2096 2409 3133 3927 4012 5083 2012
Size: 84 313 724 794 85 1071 2795
Fragments arranged by size:
2795 1071 794 724 313 85 84
BsmI GAATG_Cn'
Cuts at: 3144 3377 3470 3144
Size: 233 93 5540
Fragments arranged by size:
5540 233 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2096 2409 2677 3005 3133 3185
Size: 399 222 84 313 268 328 128 52
Cuts at: 3185 3927 4012 5083 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 313 268 222
128 85 84 52
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2393 3221 844
Size: 1549 828 3489
Fragments arranged by size:
3489 1549 828
BsrBI CCG'CTC
Cuts at: 1394 1823 3753 1394
Size: 429 1930 3507
Fragments arranged by size:
3507 1930 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2472 4361 4535 65
Size: 1183 1224 1889 174 1396
Fragments arranged by size:
1889 1396 1224 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5866
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5866
BstYI r'GATC_y
Cuts at: 1972 2034 3045 3196 3563 3948 3965 5417 5503
Size: 62 1011 151 367 385 17 1452 86
Cuts at: 5503 5515 5861 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 151 86
62 17 12
BstZ17I GTA'TAC
Cuts at: 2211 2211
Size: 5866
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 2287 2571
Size: 54 1425 50 275 18 394 63 284
Cuts at: 2571 2890 3106 3313 4202 5831 8
Size: 319 216 207 889 1629 43
Fragments arranged by size:
1629 1425 889 394 319 284 275 216
207 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2189 2625 2993 3796 1353
Size: 731 105 436 368 803 3423
Fragments arranged by size:
3423 803 731 436 368 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4453 4827 4973 387
Size: 1064 138 2864 374 146 1280
Fragments arranged by size:
2864 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2094 3131 2094
Size: 1037 4829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2768 2811 2861 3929 4526 5313 5581
Size: 393 1136 43 50 1068 597 787 268
Cuts at: 5581 1239
Size: 1524
Fragments arranged by size:
1524 1136 1068 787 597 393 268 50
43
EcoO109I rG'GnC_Cy
Cuts at: 2673 2674 2673
Size: 1 5865
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 3036 5013 1678
Size: 1358 1977 2531
Fragments arranged by size:
2531 1977 1358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4507 4539 4581 4613 1373
Size: 32 135 32 2935 32 42 32 2626
Fragments arranged by size:
2935 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3210 3456 669
Size: 988 281 1272 246 3079
Fragments arranged by size:
3079 1272 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 3456 3456
Size: 5866
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2549 2809 4980 5164 1305
Size: 1244 260 2171 184 2007
Fragments arranged by size:
2171 2007 1244 260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2226 2289 3108 10
Size: 54 1425 343 394 63 819 2768
Fragments arranged by size:
2768 1425 819 394 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2573 3824 4183 4342 518
Size: 648 1407 1251 359 159 2042
Fragments arranged by size:
2042 1407 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 2870 2914 3282 3959 5107 5352 1606
Size: 498 766 44 368 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 677 498 368 245 44
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5314
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5866
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NsiI A_TGCA'T
Cuts at: 2570 2570
Size: 5866
NspI r_CATG'y
Cuts at: 1132 1229 2987 3224 4769 1132
Size: 97 1758 237 1545 2229
Fragments arranged by size:
2229 1758 1545 237 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5866
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2531 1108
Size: 1423 4443
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2406 3935 3967 5448 5480 2374
Size: 32 1529 32 1481 32 2760
Fragments arranged by size:
2760 1529 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PspOMI G'GGCC_C
Cuts at: 2673 2673
Size: 5866
PstI C_TGCA'G
Cuts at: 830 2475 3218 830
Size: 1645 743 3478
Fragments arranged by size:
3478 1645 743
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
PvuII CAG'CTG
Cuts at: 2104 2104
Size: 5866
SacI G_AGCT'C
Cuts at: 2096 3133 2096
Size: 1037 4829
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SapI GCTCTTCn'nnn_
Cuts at: 2084 2993 2084
Size: 909 4957
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 2058 4114 5826 2058
Size: 2056 1712 2098
Fragments arranged by size:
2098 2056 1712
SfcI C'TryA_G
Cuts at: 826 1045 2471 3214 4349 5030 5221 826
Size: 219 1426 743 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 743 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3970 4871 5133 820
Size: 197 926 2027 901 262 1553
Fragments arranged by size:
2027 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 2987 3224 1132
Size: 1855 237 3774
Fragments arranged by size:
3774 1855 237
SspI AAT'ATT
Cuts at: 5 52 3790 5
Size: 47 3738 2081
Fragments arranged by size:
3738 2081 47
StuI AGG'CCT
Cuts at: 1517 2133 1517
Size: 616 5250
StyI C'CwwG_G
Cuts at: 513 1065 3305 513
Size: 552 2240 3074
Fragments arranged by size:
3074 2240 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3893 3910 4063 4248 4693 325
Size: 1255 2313 17 153 185 445 1498
Fragments arranged by size:
2313 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2056 4112 5824
Size: 274 80 33 51 1443 79 2056 1712
Cuts at: 5824 96
Size: 138
Fragments arranged by size:
2056 1712 1443 274 138 80 79 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5866
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 5866
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI