Restriction map for
pFN21AE4369
(Circular) (Six-base) MAPSORT of: pFN21AE4369.seq Check: 9671 from: 1 to: 5863
pFN21AE4369 5863 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2631 3206 1937
Size: 694 575 4594
Fragments arranged by size:
4594 694 575
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 3168 3926 5310 5578 1632
Size: 840 696 758 1384 268 1917
Fragments arranged by size:
1917 1384 840 758 696 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 2879 1166
Size: 1713 4150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5863
AlwNI CAG_nnn'CTG
Cuts at: 2397 2484 3606 5178 2397
Size: 87 1122 1572 3082
Fragments arranged by size:
3082 1572 1122 87
ApaLI G'TGCA_C
Cuts at: 2432 3920 5076 2432
Size: 1488 1156 3219
Fragments arranged by size:
3219 1488 1156
ApoI r'AATT_y
Cuts at: 1493 2276 2531 2616 2748 3172 3398 3550 5659
Size: 783 255 85 132 424 226 152 2109
Cuts at: 5659 1493
Size: 1697
Fragments arranged by size:
2109 1697 783 424 255 226 152 132
85
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2679 3188 1870
Size: 73 736 509 4545
Fragments arranged by size:
4545 736 509 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3184 4639 618
Size: 325 217 627 1397 1455 1842
Fragments arranged by size:
1842 1455 1397 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3182 1391
Size: 1791 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3156 928
Size: 971 1257 3635
Fragments arranged by size:
3635 1257 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4054 4088 1903
Size: 34 2117 34 3678
Fragments arranged by size:
3678 2117 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3033 3755 4965 3033
Size: 722 1210 3931
Fragments arranged by size:
3931 1210 722
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4474 136
Size: 107 122 71 631 605 2802 1525
Fragments arranged by size:
2802 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3075 3280 3075
Size: 205 5658
Bme1580I G_kGCm'C
Cuts at: 1790 2436 3924 5080 1790
Size: 646 1488 1156 2573
Fragments arranged by size:
2573 1488 1156 646
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3594 4552 442
Size: 416 53 83 399 24 2177 958 1753
Fragments arranged by size:
2177 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2844 2844
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3083 4523 1239
Size: 1844 1440 2579
Fragments arranged by size:
2579 1844 1440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 3084 3988 4853 5151 2525
Size: 559 904 865 298 3237
Fragments arranged by size:
3237 904 865 559 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2133 4526 882
Size: 617 634 2393 2219
Fragments arranged by size:
2393 2219 634 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 5857 5857
Size: 5863
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2334 4296 4694 4968 5115 5526 2334
Size: 1962 398 274 147 411 2671
Fragments arranged by size:
2671 1962 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2007 2037 2096 2126 1178
Size: 30 165 30 604 30 59 30 4915
Fragments arranged by size:
4915 604 165 59 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 1186
Size: 826 5037
BseYI C'CCAG_C
Cuts at: 2217 2221 3050 5066 5562 2217
Size: 4 829 2016 496 2518
Fragments arranged by size:
2518 2016 829 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1998 2531 1163
Size: 573 262 533 4495
Fragments arranged by size:
4495 573 533 262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3313 4074 5102 1540
Size: 275 186 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2436 3924 4009 5080 2436
Size: 1488 85 1071 3219
Fragments arranged by size:
3219 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
BsmBI CGTCTCn'nnnn_
Cuts at: 2999 2999
Size: 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2436 3182 3924 4009 5080 1391
Size: 399 646 746 742 85 1071 2174
Fragments arranged by size:
2174 1071 746 742 646 399 85
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 3218 844
Size: 1634 740 3489
Fragments arranged by size:
3489 1634 740
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4358 4532 65
Size: 1183 3110 174 1396
Fragments arranged by size:
3110 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4507 4694 1700
Size: 110 2697 187 2869
Fragments arranged by size:
2869 2697 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3014 3923 4935 3014
Size: 909 1012 3942
Fragments arranged by size:
3942 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5863
BstBI TT'CG_AA
Cuts at: 2317 4667 2317
Size: 2350 3513
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858
Size: 1221 367 385 17 1452 86 12 346
Cuts at: 5858 1972
Size: 1977
Fragments arranged by size:
1977 1452 1221 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3111 513
Size: 552 2046 3265
Fragments arranged by size:
3265 2046 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 3379 4173 4193 3379
Size: 794 20 5049
Fragments arranged by size:
5049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DraIII CAC_nnn'GTG
Cuts at: 3117 3117
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2192 2206
Size: 54 1425 50 275 18 198 164 14
Cuts at: 2206 2599 3310 4199 5828 8
Size: 393 711 889 1629 43
Fragments arranged by size:
1629 1425 889 711 393 275 198 164
54 50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 2813 3793 1353
Size: 1181 279 980 3423
Fragments arranged by size:
3423 1181 980 279
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4450 4824 4970 387
Size: 1064 138 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2472 3083 3168 3926 4523 5310 5578
Size: 393 840 611 85 758 597 787 268
Cuts at: 5578 1239
Size: 1524
Fragments arranged by size:
1524 840 787 758 611 597 393 268
85
EcoO109I rG'GnC_Cy
Cuts at: 2605 2605
Size: 5863
EcoRI G'AATT_C
Cuts at: 2531 2748 3172 3550 2531
Size: 217 424 378 4844
Fragments arranged by size:
4844 424 378 217
FspI TGC'GCA
Cuts at: 2868 4369 2868
Size: 1501 4362
HaeII r_GCGC'y
Cuts at: 1678 2903 5010 1678
Size: 1225 2107 2531
Fragments arranged by size:
2531 2107 1225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2980 3012 4504 4536 4578
Size: 32 135 32 1408 32 1492 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 1492 1408 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2332 3207 3453 669
Size: 988 281 394 875 246 3079
Fragments arranged by size:
3079 988 875 394 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4977 5161 1305
Size: 3672 184 2007
Fragments arranged by size:
3672 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 2194 2208 2601 10
Size: 54 1425 343 198 164 14 393 3272
Fragments arranged by size:
3272 1425 393 343 198 164 54 14
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 2879 3821 4180 4339 518
Size: 648 1263 450 942 359 159 2042
Fragments arranged by size:
2042 1263 942 648 450 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 3054 3279 3956 5104 5349 1606
Size: 615 833 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 833 677 615 245 225
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5311
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 3221 4766 1132
Size: 97 1992 1545 2229
Fragments arranged by size:
2229 1992 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5863
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2605 2605
Size: 5863
PshAI GACnn'nnGTC
Cuts at: 1613 2145 1613
Size: 532 5331
PsiI TTA'TAA
Cuts at: 2556 3433 2556
Size: 877 4986
PstI C_TGCA'G
Cuts at: 830 3047 3215 830
Size: 2217 168 3478
Fragments arranged by size:
3478 2217 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2221 3054 2221
Size: 833 5030
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 2978 4111 5823 2978
Size: 1133 1712 3018
Fragments arranged by size:
3018 1712 1133
SfcI C'TryA_G
Cuts at: 826 1045 2891 3043 3211 4346 5027 5218 826
Size: 219 1846 152 168 1135 681 191 1471
Fragments arranged by size:
1846 1471 1135 681 219 191 168 152
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 2681 3190 1872
Size: 809 509 4545
Fragments arranged by size:
4545 809 509
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2504 3099 3967 4868 5130 820
Size: 197 926 561 595 868 901 262 1553
Fragments arranged by size:
1553 926 901 868 595 561 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2050 2100 2574 2779 3302 513
Size: 552 985 50 474 205 523 3074
Fragments arranged by size:
3074 985 552 523 474 205 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2100 2157 2231 3890 3907 4060 4245
Size: 1255 520 57 74 1659 17 153 185
Cuts at: 4245 4690 325
Size: 445 1498
Fragments arranged by size:
1659 1498 1255 520 445 185 153 74
57 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2092 2958 2976
Size: 274 80 33 51 1443 115 866 18
Cuts at: 2976 2991 3155 4109 5821 96
Size: 15 164 954 1712 138
Fragments arranged by size:
1712 1443 954 866 274 164 138 115
80 51 33 18 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 5823 5855 2007
Size: 32 3784 32 2015
Fragments arranged by size:
3784 2015 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2609 3058 2609
Size: 449 5414
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 2679 3188 1870
Size: 809 509 4545
Fragments arranged by size:
4545 809 509
XmnI GAAnn'nnTTC
Cuts at: 2584 3992 2584
Size: 1408 4455
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI