Restriction map for
pFN21AE4368
(Circular) (Six-base) MAPSORT of: pFN21AE4368.seq Check: 390 from: 1 to: 5863
pFN21AE4368 5863 bp
With 182 enzymes: SgfI *
March 28, 2012 13:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2616 3206 1937
Size: 679 590 4594
Fragments arranged by size:
4594 679 590
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3926 5310 5578 1632
Size: 2294 1384 268 1917
Fragments arranged by size:
2294 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2912 820
Size: 197 1895 3771
Fragments arranged by size:
3771 1895 197
AflIII A'CryG_T
Cuts at: 1203 2805 4762 5739 1203
Size: 1602 1957 977 1327
Fragments arranged by size:
1957 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5863
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2682 2714 2997 3029 3069 3101 2682
Size: 32 283 32 40 32 5444
Fragments arranged by size:
5444 283 40 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2978 3606 5178 2978
Size: 628 1572 3663
Fragments arranged by size:
3663 1572 628
ApaLI G'TGCA_C
Cuts at: 3920 5076 3920
Size: 1156 4707
ApoI r'AATT_y
Cuts at: 1493 2282 3172 3398 3550 5659 1493
Size: 789 890 226 152 2109 1697
Fragments arranged by size:
2109 1697 890 789 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 3188 1870
Size: 73 1245 4545
Fragments arranged by size:
4545 1245 73
AvrII C'CTAG_G
Cuts at: 2857 2857
Size: 5863
BamHI G'GATC_C
Cuts at: 2997 3193 3560 2997
Size: 196 367 5300
Fragments arranged by size:
5300 367 196
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 3184 4639 618
Size: 325 217 627 289 1108 1455 1842
Fragments arranged by size:
1842 1455 1108 627 325 289 217
BanII G_rGCy'C
Cuts at: 1391 2136 3182 1391
Size: 745 1046 4072
Fragments arranged by size:
4072 1046 745
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2016 2050 4054 4088 1903
Size: 34 79 34 2004 34 3678
Fragments arranged by size:
3678 2004 79 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4474 136
Size: 107 122 71 631 605 2802 1525
Fragments arranged by size:
2802 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5858 5858
Size: 5863
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 2079 3924 5080 1790
Size: 289 1845 1156 2573
Fragments arranged by size:
2573 1845 1156 289
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2791 3594 4552
Size: 416 53 83 399 24 1374 803 958
Cuts at: 4552 442
Size: 1753
Fragments arranged by size:
1753 1374 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 2653 2597
Size: 56 5807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 3095 3096 4523 1239
Size: 792 1064 1 1427 2579
Fragments arranged by size:
2579 1427 1064 792 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 3988 4853 5151 2333
Size: 1655 865 298 3045
Fragments arranged by size:
3045 1655 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2641 4526 882
Size: 617 1142 1885 2219
Fragments arranged by size:
2219 1885 1142 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 2759 5857 2759
Size: 3098 2765
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4296 4694 4968 5115 5526 4296
Size: 398 274 147 411 4633
Fragments arranged by size:
4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3069 3099 1178
Size: 30 165 30 1666 30 3942
Fragments arranged by size:
3942 1666 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 1186
Size: 1013 4850
BseYI C'CCAG_C
Cuts at: 2770 5066 5562 2770
Size: 2296 496 3071
Fragments arranged by size:
3071 2296 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2056 1163
Size: 573 320 4970
Fragments arranged by size:
4970 573 320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3313 4074 5102 1540
Size: 275 186 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3924 4009 5080 3924
Size: 85 1071 4707
Fragments arranged by size:
4707 1071 85
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2136 3182 3924 4009 5080 1391
Size: 399 289 57 1046 742 85 1071 2174
Fragments arranged by size:
2174 1071 1046 742 399 289 85 57
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 2326 3126 3752 5482 2326
Size: 800 626 1730 2707
Fragments arranged by size:
2707 1730 800 626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3218 844
Size: 2374 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3750 1394
Size: 429 1927 3507
Fragments arranged by size:
3507 1927 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2775 4358 4532 65
Size: 1183 1527 1583 174 1396
Fragments arranged by size:
1583 1527 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2379 4507 4694 1700
Size: 110 569 2128 187 2869
Fragments arranged by size:
2869 2128 569 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5863
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 2087 2087
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 2493 2997 3193 3560 3945 3962 5414 5500
Size: 521 504 196 367 385 17 1452 86
Cuts at: 5500 5512 5858 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 521 504 385 367 346 196
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2211 2211
Size: 5863
BtgI C'CryG_G
Cuts at: 513 1065 3094 513
Size: 552 2029 3282
Fragments arranged by size:
3282 2029 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 3151 3379 4173 4193 3151
Size: 228 794 20 4821
Fragments arranged by size:
4821 794 228 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2955 3310 4199
Size: 54 1425 50 275 18 1125 355 889
Cuts at: 4199 5828 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1125 889 355 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 2609 2651 2682 3144 3793 1353
Size: 739 517 42 31 462 649 3423
Fragments arranged by size:
3423 739 649 517 462 42 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4450 4824 4970 387
Size: 1064 138 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 3095 3096 3926 4523 5310 5578
Size: 393 399 1064 1 830 597 787 268
Cuts at: 5578 1239
Size: 1524
Fragments arranged by size:
1524 1064 830 787 597 399 393 268
1
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 5010 1678
Size: 3332 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4504 4536 4578 4610 1373
Size: 32 135 32 2932 32 42 32 2626
Fragments arranged by size:
2932 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4977 5161 1305
Size: 3672 184 2007
Fragments arranged by size:
3672 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2957 10
Size: 54 1425 343 1125 2916
Fragments arranged by size:
2916 1425 1125 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2765 3093 3821 4180 4339 518
Size: 648 1599 328 728 359 159 2042
Fragments arranged by size:
2042 1599 728 648 359 328 159
MspA1I CmG'CkG
Cuts at: 1606 3052 3279 3956 5104 5349 1606
Size: 1446 227 677 1148 245 2120
Fragments arranged by size:
2120 1446 1148 677 245 227
NcoI C'CATG_G
Cuts at: 513 1065 3094 513
Size: 552 2029 3282
Fragments arranged by size:
3282 2029 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2239 2809 3221 4766 1132
Size: 97 1010 570 412 1545 2229
Fragments arranged by size:
2229 1545 1010 570 412 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 2805 4762 2805
Size: 1957 3906
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5863
PfoI T'CCnGG_A
Cuts at: 3111 3111
Size: 5863
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3069 3101 3932 3964 5445 5477 3069
Size: 32 831 32 1481 32 3455
Fragments arranged by size:
3455 1481 831 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PstI C_TGCA'G
Cuts at: 830 3146 3215 830
Size: 2316 69 3478
Fragments arranged by size:
3478 2316 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 3052 3052
Size: 5863
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SapI GCTCTTCn'nnn_
Cuts at: 2609 2651 2682 2609
Size: 42 31 5790
Fragments arranged by size:
5790 42 31
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SfcI C'TryA_G
Cuts at: 826 1045 3142 3211 4346 5027 5218 826
Size: 219 2097 69 1135 681 191 1471
Fragments arranged by size:
2097 1471 1135 681 219 191 69
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 2912 3967 4868 5130 820
Size: 197 926 405 564 1055 901 262 1553
Fragments arranged by size:
1553 1055 926 901 564 405 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 3221 1132
Size: 2089 3774
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 2862 1517
Size: 1345 4518
StyI C'CwwG_G
Cuts at: 513 1065 2119 2702 2857 3094 3302 513
Size: 552 1054 583 155 237 208 3074
Fragments arranged by size:
3074 1054 583 552 237 208 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3890 3907 4060 4245 4690 325
Size: 1255 2310 17 153 185 445 1498
Fragments arranged by size:
2310 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 4109 5821
Size: 274 80 33 51 1443 355 1777 1712
Cuts at: 5821 96
Size: 138
Fragments arranged by size:
1777 1712 1443 355 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2294 2326 5823 5855 2294
Size: 32 3497 32 2302
Fragments arranged by size:
3497 2302 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI