Restriction map for
pFN21AE4363
(Circular) (Six-base) MAPSORT of: pFN21AE4363.seq Check: 8178 from: 1 to: 5848
pFN21AE4363 5848 bp
With 182 enzymes: SgfI *
March 28, 2012 13:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2028 3089 2028
Size: 1061 4787
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 2716 3169 2716
Size: 453 5395
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3911 5295 5563 1632
Size: 2279 1384 268 1917
Fragments arranged by size:
2279 1917 1384 268
AfeI AGC'GCT
Cuts at: 2759 2851 2759
Size: 92 5756
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 2188 4747 5724 1203
Size: 985 2559 977 1327
Fragments arranged by size:
2559 1327 985 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2164 2363 2432 1184
Size: 980 199 69 4600
Fragments arranged by size:
4600 980 199 69
AlwNI CAG_nnn'CTG
Cuts at: 2032 2069 2749 3043 3063 3591 5163 2032
Size: 37 680 294 20 528 1572 2717
Fragments arranged by size:
2717 1572 680 528 294 37 20
ApaI G_GGCC'C
Cuts at: 2111 2557 2111
Size: 446 5402
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 3157 3383 3535 5644 1493
Size: 1664 226 152 2109 1697
Fragments arranged by size:
2109 1697 1664 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 2549 3092 3173 1870
Size: 73 606 543 81 4545
Fragments arranged by size:
4545 606 543 81 73
AvrII C'CTAG_G
Cuts at: 2158 2158
Size: 5848
BamHI G'GATC_C
Cuts at: 2655 3178 3545 2655
Size: 523 367 4958
Fragments arranged by size:
4958 523 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2716 3052 3169 4624
Size: 325 217 627 334 595 336 117 1455
Cuts at: 4624 618
Size: 1842
Fragments arranged by size:
1842 1455 627 595 336 334 325 217
117
BanII G_rGCy'C
Cuts at: 1391 2111 2158 2557 2644 3167 1391
Size: 720 47 399 87 523 4072
Fragments arranged by size:
4072 720 523 399 87 47
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4039 4073 1903
Size: 34 2102 34 3678
Fragments arranged by size:
3678 2102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3140 3740 4950 3140
Size: 600 1210 4038
Fragments arranged by size:
4038 1210 600
BclI T'GATC_A
Cuts at: 1548 2646 1548
Size: 1098 4750
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2258 2561 4459
Size: 107 122 71 631 605 586 303 1898
Cuts at: 4459 136
Size: 1525
Fragments arranged by size:
1898 1525 631 605 586 303 122 107
71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 2754 3265 2754
Size: 511 5337
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2111 2557 3057 3909 5065 1790
Size: 314 7 446 500 852 1156 2573
Fragments arranged by size:
2573 1156 852 500 446 314 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 3579 4537
Size: 416 53 83 399 24 912 1250 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1250 958 912 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2687 1056
Size: 1631 4217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2312 2543 2954 3107 4508 1239
Size: 914 159 231 411 153 1401 2579
Fragments arranged by size:
2579 1401 914 411 231 159 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3973 4838 5136 3973
Size: 865 298 4685
Fragments arranged by size:
4685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2829 4281 4679 4953 5100 5511 2829
Size: 1452 398 274 147 411 3166
Fragments arranged by size:
3166 1452 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 1178
Size: 30 165 30 882 30 4711
Fragments arranged by size:
4711 882 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5848
BseYI C'CCAG_C
Cuts at: 2788 2805 3036 3063 5051 5547 2788
Size: 17 231 27 1988 496 3089
Fragments arranged by size:
3089 1988 496 231 27 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2170 1163
Size: 573 434 4841
Fragments arranged by size:
4841 573 434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2837 3909 3994 5065 2837
Size: 1072 85 1071 3620
Fragments arranged by size:
3620 1072 1071 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2111 2158 2557 2644 2837 3057
Size: 399 314 7 47 399 87 193 220
Cuts at: 3057 3167 3909 3994 5065 1391
Size: 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 399 314 220 193
110 87 85 47 7
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 3089 3203 844
Size: 1184 1061 114 3489
Fragments arranged by size:
3489 1184 1061 114
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2141 2164 2970 4343 4517 65
Size: 1183 893 23 806 1373 174 1396
Fragments arranged by size:
1396 1373 1183 893 806 174 23
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2032 2143 794
Size: 1238 111 4499
Fragments arranged by size:
4499 1238 111
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstYI r'GATC_y
Cuts at: 1972 2587 2655 3178 3545 3930 3947 5399 5485
Size: 615 68 523 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 615 523 385 367 346 86
68 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2838 513
Size: 552 939 834 3523
Fragments arranged by size:
3523 939 834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 2067 2153 2700 3364 4158 4178 2033
Size: 34 86 547 664 794 20 3703
Fragments arranged by size:
3703 794 664 547 86 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2531 4855 809
Size: 507 1215 2324 1802
Fragments arranged by size:
2324 1802 1215 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 3295 4184
Size: 54 1425 50 275 18 420 1045 889
Cuts at: 4184 5813 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1045 889 420 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3778 1353
Size: 2425 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2153 2312 2543 2954 3107 3911 4508
Size: 393 521 159 231 411 153 804 597
Cuts at: 4508 5295 5563 1239
Size: 787 268 1524
Fragments arranged by size:
1524 804 787 597 521 411 393 268
231 159 153
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 2294
Size: 5848
EcoO109I rG'GnC_Cy
Cuts at: 2107 2108 2500 2553 2554 3088 2107
Size: 1 392 53 1 534 4867
Fragments arranged by size:
4867 534 392 53 1 1
EcoRI G'AATT_C
Cuts at: 3157 3535 3157
Size: 378 5470
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2761 2853 4995 1678
Size: 1083 92 2142 2531
Fragments arranged by size:
2531 2142 1083 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373
Size: 32 135 32 2917 32 42 32 2626
Fragments arranged by size:
2917 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 2720 3173 2720
Size: 453 5395
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 10
Size: 54 1425 343 420 3606
Fragments arranged by size:
3606 1425 420 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2146 2414 2915 3806 4165 4324 518
Size: 648 980 268 501 891 359 159 2042
Fragments arranged by size:
2042 980 891 648 501 359 268 159
MspA1I CmG'CkG
Cuts at: 1606 2809 3040 3264 3941 5089 5334 1606
Size: 1203 231 224 677 1148 245 2120
Fragments arranged by size:
2120 1203 1148 677 245 231 224
NcoI C'CATG_G
Cuts at: 513 1065 2004 2838 513
Size: 552 939 834 3523
Fragments arranged by size:
3523 939 834 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 2683 1052
Size: 1631 4217
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2192 2800 2914 3206 4751 1132
Size: 97 963 608 114 292 1545 2229
Fragments arranged by size:
2229 1545 963 608 292 114 97
PasI CC'CwG_GG
Cuts at: 1348 2104 3057 1348
Size: 756 953 4139
Fragments arranged by size:
4139 953 756
PciI A'CATG_T
Cuts at: 2188 4747 2188
Size: 2559 3289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2131 2749 3063 1108
Size: 1023 618 314 3893
Fragments arranged by size:
3893 1023 618 314
PfoI T'CCnGG_A
Cuts at: 2268 2289 2268
Size: 21 5827
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2500 2500
Size: 5848
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PspOMI G'GGCC_C
Cuts at: 2107 2553 2107
Size: 446 5402
PstI C_TGCA'G
Cuts at: 830 2028 2639 3200 830
Size: 1198 611 561 3478
Fragments arranged by size:
3478 1198 611 561
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2809 3040 2809
Size: 231 5617
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SanDI GG'GwC_CC
Cuts at: 2500 2500
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 2024 2171 2635 3196 4331 5012 5203
Size: 219 979 147 464 561 1135 681 191
Cuts at: 5203 826
Size: 1471
Fragments arranged by size:
1471 1135 979 681 561 464 219 191
147
SfiI GGCCn_nnn'nGGCC
Cuts at: 2258 2258
Size: 5848
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 2551 3175 1872
Size: 679 624 4545
Fragments arranged by size:
4545 679 624
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3952 4853 5115 820
Size: 197 926 2009 901 262 1553
Fragments arranged by size:
2009 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 2800 3206 1132
Size: 1668 406 3774
Fragments arranged by size:
3774 1668 406
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 2569 2975 1517
Size: 1052 406 4390
Fragments arranged by size:
4390 1052 406
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 2158 2253 2673 2838 2858
Size: 552 939 87 67 95 420 165 20
Cuts at: 2858 3287 513
Size: 429 3074
Fragments arranged by size:
3074 939 552 429 420 165 95 87
67 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2939 3875 3892 4045 4230 4675 325
Size: 1255 1359 936 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 936 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4094 5806 96
Size: 274 80 33 51 1443 2117 1712 138
Fragments arranged by size:
2117 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2285 2317 5808 5840 2285
Size: 32 3491 32 2293
Fragments arranged by size:
3491 2293 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 3109 1316
Size: 912 881 4055
Fragments arranged by size:
4055 912 881
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2089 2567 2978 2089
Size: 478 411 4959
Fragments arranged by size:
4959 478 411
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 2549 3173 1870
Size: 679 624 4545
Fragments arranged by size:
4545 679 624
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfoI SrfI SwaI