Restriction map for
pFN21AE4362
(Circular) (Six-base) MAPSORT of: pFN21AE4362.seq Check: 9855 from: 1 to: 5845
pFN21AE4362 5845 bp
With 182 enzymes: SgfI *
March 28, 2012 13:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2819 2819
Size: 5845
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3908 5292 5560 1632
Size: 2276 1384 268 1917
Fragments arranged by size:
2276 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 2474 1166
Size: 1308 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5845
AlwNI CAG_nnn'CTG
Cuts at: 3588 5160 3588
Size: 1572 4273
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 2174 2335 2515 3038 3154 3380 3532 5641
Size: 681 161 180 523 116 226 152 2109
Cuts at: 5641 1493
Size: 1697
Fragments arranged by size:
2109 1697 681 523 226 180 161 152
116
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 1870
Size: 73 1227 4545
Fragments arranged by size:
4545 1227 73
AvrII C'CTAG_G
Cuts at: 2620 2620
Size: 5845
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 3166 4621 618
Size: 325 217 627 222 1157 1455 1842
Fragments arranged by size:
1842 1455 1157 627 325 222 217
BanII G_rGCy'C
Cuts at: 1391 3164 1391
Size: 1773 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 928
Size: 971 501 4373
Fragments arranged by size:
4373 971 501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 2377 1548
Size: 829 5016
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4456 136
Size: 107 122 71 631 605 2784 1525
Fragments arranged by size:
2784 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2012 3906 5062 1790
Size: 222 1894 1156 2573
Fragments arranged by size:
2573 1894 1156 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2189 3576 4534
Size: 416 53 83 399 24 772 1387 958
Cuts at: 4534 442
Size: 1753
Fragments arranged by size:
1753 1387 958 772 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4505 1239
Size: 3266 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 2487 5839 2487
Size: 3352 2493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3652 4034 4669 275
Size: 53 83 186 936 2119 382 635 1451
Fragments arranged by size:
2119 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 1186
Size: 1768 4077
BseYI C'CCAG_C
Cuts at: 2877 5048 5544 2877
Size: 2171 496 3178
Fragments arranged by size:
3178 2171 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2847 1163
Size: 573 1111 4161
Fragments arranged by size:
4161 1111 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2956 3295 4056 5084 1540
Size: 275 186 955 339 761 1028 2301
Fragments arranged by size:
2301 1028 955 761 339 275 186
BsiHKAI G_wGCw'C
Cuts at: 3906 3991 5062 3906
Size: 85 1071 4689
Fragments arranged by size:
4689 1071 85
BsmI GAATG_Cn'
Cuts at: 2118 3356 3449 2118
Size: 1238 93 4514
Fragments arranged by size:
4514 1238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 3164 3906 3991 5062 1391
Size: 399 222 1152 742 85 1071 2174
Fragments arranged by size:
2174 1152 1071 742 399 222 85
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 2107 3734 5464 2107
Size: 1627 1730 2488
Fragments arranged by size:
2488 1730 1627
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2210 2397 2423 2819 3200 844
Size: 1366 187 26 396 381 3489
Fragments arranged by size:
3489 1366 396 381 187 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3732 1394
Size: 429 1909 3507
Fragments arranged by size:
3507 1909 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 4340 4514 65
Size: 1183 879 2213 174 1396
Fragments arranged by size:
2213 1396 1183 879 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4489 4676 1700
Size: 110 2679 187 2869
Fragments arranged by size:
2869 2679 187 110
BsrGI T'GTAC_A
Cuts at: 96 2864 96
Size: 2768 3077
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5845
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 2587 3058 1184
Size: 1403 471 3971
Fragments arranged by size:
3971 1403 471
BstXI CCAn_nnnn'nTGG
Cuts at: 2444 2444
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 2834 3175 3542 3927 3944 5396 5482 5494
Size: 862 341 367 385 17 1452 86 12
Cuts at: 5494 5840 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 862 385 367 346 341 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4354
Fragments arranged by size:
4354 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2029 487
Size: 683 56 803 4303
Fragments arranged by size:
4303 803 683 56
BtsI GCAGTG_nn'
Cuts at: 3361 4155 4175 3361
Size: 794 20 5031
Fragments arranged by size:
5031 794 20
ClaI AT'CG_AT
Cuts at: 1554 2166 1554
Size: 612 5233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2953 3292
Size: 54 1425 50 275 18 376 747 339
Cuts at: 3292 4181 5810 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 747 376 339 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2953 3292 1537
Size: 275 1141 339 4090
Fragments arranged by size:
4090 1141 339 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3775 1353
Size: 720 1702 3423
Fragments arranged by size:
3423 1702 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3908 4505 5292 5560 1239
Size: 393 2276 597 787 268 1524
Fragments arranged by size:
2276 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2182 2182
Size: 5845
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
EcoRV GAT'ATC
Cuts at: 2485 2485
Size: 5845
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 4992 1678
Size: 3314 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2515 2547 4486 4518 4560
Size: 32 135 32 943 32 1939 32 42
Cuts at: 4560 4592 1373
Size: 32 2626
Fragments arranged by size:
2626 1939 943 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2966 4959 5143 1305
Size: 1661 1993 184 2007
Fragments arranged by size:
2007 1993 1661 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2208 10
Size: 54 1425 343 376 3647
Fragments arranged by size:
3647 1425 376 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 2296 2474 3803 4162 4321 518
Size: 648 985 145 178 1329 359 159 2042
Fragments arranged by size:
2042 1329 985 648 359 178 159 145
MspA1I CmG'CkG
Cuts at: 1606 3261 3938 5086 5331 1606
Size: 1655 677 1148 245 2120
Fragments arranged by size:
2120 1655 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4354
Fragments arranged by size:
4354 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 3203 4748 1132
Size: 97 1974 1545 2229
Fragments arranged by size:
2229 1974 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2725 1348
Size: 1377 4468
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2049 1108
Size: 941 4904
PfoI T'CCnGG_A
Cuts at: 2772 2772
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2255 1613
Size: 642 5203
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PstI C_TGCA'G
Cuts at: 830 2221 3197 830
Size: 1391 976 3478
Fragments arranged by size:
3478 1391 976
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SexAI A'CCwGG_T
Cuts at: 2263 2263
Size: 5845
SfcI C'TryA_G
Cuts at: 826 1045 2217 3193 4328 5009 5200 826
Size: 219 1172 976 1135 681 191 1471
Fragments arranged by size:
1471 1172 1135 976 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2100 2583 2620 2796 3112
Size: 552 939 9 87 483 37 176 316
Cuts at: 3112 3284 513
Size: 172 3074
Fragments arranged by size:
3074 939 552 483 316 176 172 87
37 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3872 3889 4042 4227 4672 325
Size: 1255 2292 17 153 185 445 1498
Fragments arranged by size:
2292 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2453 2466 2864
Size: 274 80 33 51 1443 476 13 398
Cuts at: 2864 2888 4091 5803 96
Size: 24 1203 1712 138
Fragments arranged by size:
1712 1443 1203 476 398 274 138 80
51 33 24 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5805 5837 5805
Size: 32 5813
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2803 2803
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI