Restriction map for
pFN21AE4360
(Circular) (Six-base) MAPSORT of: pFN21AE4360.seq Check: 2107 from: 1 to: 5842
pFN21AE4360 5842 bp
With 182 enzymes: SgfI *
March 28, 2012 14:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2882 3652 278
Size: 53 83 186 936 1346 770 2468
Fragments arranged by size:
2468 1346 936 770 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3185 1937
Size: 1248 4594
Acc65I G'GTAC_C
Cuts at: 3163 3163
Size: 5842
AclI AA'CG_TT
Cuts at: 1563 3969 4342 1563
Size: 2406 373 3063
Fragments arranged by size:
3063 2406 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 3905 5289 5557 1632
Size: 578 1695 1384 268 1917
Fragments arranged by size:
1917 1695 1384 578 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5645
AflIII A'CryG_T
Cuts at: 1203 4741 5718 1203
Size: 3538 977 1327
Fragments arranged by size:
3538 1327 977
AgeI A'CCGG_T
Cuts at: 4673 4673
Size: 5842
AhdI GACnn_n'nnGTC
Cuts at: 4571 4571
Size: 5842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2334 2603 1184
Size: 1150 269 4423
Fragments arranged by size:
4423 1150 269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2685 2717 2685
Size: 32 5810
AlwNI CAG_nnn'CTG
Cuts at: 2188 2764 2930 3585 5157 2188
Size: 576 166 655 1572 2873
Fragments arranged by size:
2873 1572 655 576 166
ApaLI G'TGCA_C
Cuts at: 3899 5055 3899
Size: 1156 4686
ApoI r'AATT_y
Cuts at: 1493 3005 3151 3377 3529 5638 1493
Size: 1512 146 226 152 2109 1697
Fragments arranged by size:
2109 1697 1512 226 152 146
AseI AT'TA_AT
Cuts at: 160 1032 4396 160
Size: 872 3364 1606
Fragments arranged by size:
3364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
AvaI C'yCGr_G
Cuts at: 1870 1943 2370 3167 1870
Size: 73 427 797 4545
Fragments arranged by size:
4545 797 427 73
BamHI G'GATC_C
Cuts at: 3172 3539 3172
Size: 367 5475
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2489 2528 3163 4618 618
Size: 325 217 627 702 39 635 1455 1842
Fragments arranged by size:
1842 1455 702 635 627 325 217 39
BanII G_rGCy'C
Cuts at: 1391 2181 2652 2914 3161 1391
Size: 790 471 262 247 4072
Fragments arranged by size:
4072 790 471 262 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2198 928
Size: 971 299 4572
Fragments arranged by size:
4572 971 299
BbvCI CC'TCA_GC
Cuts at: 2028 2028
Size: 5842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3011 3045 4033 4067 1903
Size: 34 1074 34 988 34 3678
Fragments arranged by size:
3678 1074 988 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3734 4944 3734
Size: 1210 4632
BclI T'GATC_A
Cuts at: 1548 2341 1548
Size: 793 5049
BfrBI ATG'CAT
Cuts at: 2462 2462
Size: 5842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2779 4453 136
Size: 107 122 71 631 605 1107 1674 1525
Fragments arranged by size:
1674 1525 1107 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2511 5837 2511
Size: 3326 2516
BlpI GC'TnA_GC
Cuts at: 2814 3259 2814
Size: 445 5397
Bme1580I G_kGCm'C
Cuts at: 1790 2202 2494 3085 3903 5059 1790
Size: 412 292 591 818 1156 2573
Fragments arranged by size:
2573 1156 818 591 412 292
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5842
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2260 2319 2331
Size: 416 53 83 399 24 843 59 12
Cuts at: 2331 2488 2950 3573 4531 442
Size: 157 462 623 958 1753
Fragments arranged by size:
1753 958 843 623 462 416 399 157
83 59 53 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4502 1239
Size: 3263 2579
Bpu10I CC'TnA_GC
Cuts at: 2028 2799 3033 3057 2028
Size: 771 234 24 4813
Fragments arranged by size:
4813 771 234 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3967 4832 5130 3967
Size: 865 298 4679
Fragments arranged by size:
4679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4505 882
Size: 617 3006 2219
Fragments arranged by size:
3006 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3633 493
Size: 711 2429 2702
Fragments arranged by size:
2702 2429 711
BsaBI GATnn'nnATC
Cuts at: 5836 5836
Size: 5842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 2058 2879 3649
Size: 53 83 186 936 512 13 821 770
Cuts at: 3649 4031 4666 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 821 770 635 512 382 186
83 53 13
BsaWI w'CCGG_w
Cuts at: 3000 4275 4673 4947 5094 5505 3000
Size: 1275 398 274 147 411 3337
Fragments arranged by size:
3337 1275 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5617
Fragments arranged by size:
5617 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 2495 2624 1186
Size: 1202 107 129 4404
Fragments arranged by size:
4404 1202 129 107
BseYI C'CCAG_C
Cuts at: 2181 2302 2485 2601 2930 5045 5541 2181
Size: 121 183 116 329 2115 496 2482
Fragments arranged by size:
2482 2115 496 329 183 121 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2528 2942 1163
Size: 573 792 414 4063
Fragments arranged by size:
4063 792 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 4053 5081 1540
Size: 275 186 1291 761 1028 2301
Fragments arranged by size:
2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2718 3903 3988 5059 2718
Size: 1185 85 1071 3501
Fragments arranged by size:
3501 1185 1071 85
BsmI GAATG_Cn'
Cuts at: 3353 3446 3353
Size: 93 5749
BsmBI CGTCTCn'nnnn_
Cuts at: 2870 2870
Size: 5842
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2202 2494 2652 2718 2914 3085
Size: 399 391 21 292 158 66 196 171
Cuts at: 3085 3161 3903 3988 5059 1391
Size: 76 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 292 196 171
158 85 76 66 21
BspEI T'CCGG_A
Cuts at: 3000 5505 3000
Size: 2505 3337
BspHI T'CATG_A
Cuts at: 2838 3731 5461 2838
Size: 893 1730 3219
Fragments arranged by size:
3219 1730 893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3197 844
Size: 2353 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2980 3729 1394
Size: 429 1157 749 3507
Fragments arranged by size:
3507 1157 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4337 4511 65
Size: 1183 3089 174 1396
Fragments arranged by size:
3089 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4486 4673 1700
Size: 110 2676 187 2869
Fragments arranged by size:
2869 2676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5842
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5842
BssSI C'ACGA_G
Cuts at: 3902 4914 3902
Size: 1012 4830
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2366 794
Size: 1572 4270
BstBI TT'CG_AA
Cuts at: 4646 4646
Size: 5842
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5842
BstYI r'GATC_y
Cuts at: 1972 2442 2511 3172 3539 3924 3941 5393 5479
Size: 470 69 661 367 385 17 1452 86
Cuts at: 5479 5491 5837 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 661 470 385 367 346 86
69 17 12
Bsu36I CC'TnA_GG
Cuts at: 3090 3090
Size: 5842
BtgI C'CryG_G
Cuts at: 513 1065 2895 513
Size: 552 1830 3460
Fragments arranged by size:
3460 1830 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3121 487
Size: 683 56 1895 3208
Fragments arranged by size:
3208 1895 683 56
BtsI GCAGTG_nn'
Cuts at: 2020 2560 2762 3358 4152 4172 2020
Size: 540 202 596 794 20 3690
Fragments arranged by size:
3690 794 596 540 202 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5807
DraI TTT'AAA
Cuts at: 3146 3146
Size: 5842
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4849 809
Size: 507 3533 1802
Fragments arranged by size:
3533 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3289 4178 5807
Size: 54 1425 50 275 18 1459 889 1629
Cuts at: 5807 8
Size: 43
Fragments arranged by size:
1629 1459 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3289 1537
Size: 275 1477 4090
Fragments arranged by size:
4090 1477 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3772 1353
Size: 2419 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 4429 4803 4949 387
Size: 1064 138 480 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 480 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 3905 4502 5289 5557 1239
Size: 393 578 1695 597 787 268 1524
Fragments arranged by size:
1695 1524 787 597 578 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2333 3046 2333
Size: 713 5129
EcoRI G'AATT_C
Cuts at: 3151 3529 3151
Size: 378 5464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 5842
FspI TGC'GCA
Cuts at: 4348 4348
Size: 5842
HaeII r_GCGC'y
Cuts at: 1678 4989 1678
Size: 3311 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 2816 2848 4483
Size: 32 135 32 1028 32 184 32 1635
Cuts at: 4483 4515 4557 4589 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1635 1028 184 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 3432 669
Size: 988 281 1248 246 3079
Fragments arranged by size:
3079 1248 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5842
HpaI GTT'AAC
Cuts at: 3432 3432
Size: 5842
KpnI G_GTAC'C
Cuts at: 3167 3167
Size: 5842
MfeI C'AATT_G
Cuts at: 3441 3441
Size: 5842
MluI A'CGCG_T
Cuts at: 5718 5718
Size: 5842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2388 4956 5140 1305
Size: 1083 2568 184 2007
Fragments arranged by size:
2568 2007 1083 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4020
Fragments arranged by size:
4020 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3800 4159 4318 518
Size: 648 2634 359 159 2042
Fragments arranged by size:
2634 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2956 3258 3935 5083 5328 1606
Size: 507 843 302 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 677 507 302 245
NcoI C'CATG_G
Cuts at: 513 1065 2895 513
Size: 552 1830 3460
Fragments arranged by size:
3460 1830 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5842
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5842
NotI GC'GGCC_GC
Cuts at: 3289 3289
Size: 5842
NsiI A_TGCA'T
Cuts at: 2464 2464
Size: 5842
NspI r_CATG'y
Cuts at: 1132 1229 2786 3200 4745 1132
Size: 97 1557 414 1545 2229
Fragments arranged by size:
2229 1557 1545 414 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5842
PciI A'CATG_T
Cuts at: 4741 4741
Size: 5842
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2901 1108
Size: 1793 4049
PfoI T'CCnGG_A
Cuts at: 2329 2329
Size: 5842
PmeI GTTT'AAAC
Cuts at: 3146 3146
Size: 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2043 3911 3943 5424 5456 2011
Size: 32 1868 32 1481 32 2397
Fragments arranged by size:
2397 1868 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2333 3046 2333
Size: 713 5129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5842
PsiI TTA'TAA
Cuts at: 3412 3412
Size: 5842
PspXI vC'TCGA_Gb
Cuts at: 2370 2370
Size: 5842
PstI C_TGCA'G
Cuts at: 830 2118 2313 3070 3194 830
Size: 1288 195 757 124 3478
Fragments arranged by size:
3478 1288 757 195 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5842
PvuII CAG'CTG
Cuts at: 2956 2956
Size: 5842
SalI G'TCGA_C
Cuts at: 1936 3184 1936
Size: 1248 4594
SbfI CC_TGCA'GG
Cuts at: 3194 3194
Size: 5842
ScaI AGT'ACT
Cuts at: 2727 4090 5802 2727
Size: 1363 1712 2767
Fragments arranged by size:
2767 1712 1363
SfcI C'TryA_G
Cuts at: 826 1045 2114 2309 3066 3190 4325 5006 5197
Size: 219 1069 195 757 124 1135 681 191
Cuts at: 5197 826
Size: 1471
Fragments arranged by size:
1471 1135 1069 757 681 219 195 191
124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5842
SmaI CCC'GGG
Cuts at: 1872 3169 1872
Size: 1297 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2370 3946 4847 5109 820
Size: 197 926 427 1576 901 262 1553
Fragments arranged by size:
1576 1553 926 901 427 262 197
SnaBI TAC'GTA
Cuts at: 493 3633 493
Size: 3140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5842
SphI G_CATG'C
Cuts at: 1132 3200 1132
Size: 2068 3774
SspI AAT'ATT
Cuts at: 5 52 3766 5
Size: 47 3714 2081
Fragments arranged by size:
3714 2081 47
StuI AGG'CCT
Cuts at: 1517 2627 1517
Size: 1110 4732
StyI C'CwwG_G
Cuts at: 513 1065 2501 2895 3281 513
Size: 552 1436 394 386 3074
Fragments arranged by size:
3074 1436 552 394 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2116 2422 2477 2494 3869 3886 4039
Size: 1255 536 306 55 17 1375 17 153
Cuts at: 4039 4224 4669 325
Size: 185 445 1498
Fragments arranged by size:
1498 1375 1255 536 445 306 185 153
55 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2664 2725 4088
Size: 274 80 33 51 1443 687 61 1363
Cuts at: 4088 5800 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1363 687 274 138 80 61
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5802 5834 5802
Size: 32 5810
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5842
XbaI T'CTAG_A
Cuts at: 3178 3178
Size: 5842
XhoI C'TCGA_G
Cuts at: 1943 2370 1943
Size: 427 5415
XmaI C'CCGG_G
Cuts at: 1870 3167 1870
Size: 1297 4545
XmnI GAAnn'nnTTC
Cuts at: 3971 3971
Size: 5842
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2880 3650 276
Size: 53 83 186 936 1346 770 2468
Fragments arranged by size:
2468 1346 936 770 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI