Restriction map for
pFN21AE4349
(Circular) (Six-base) MAPSORT of: pFN21AE4349.seq Check: 6501 from: 1 to: 5797
pFN21AE4349 5797 bp
With 182 enzymes: SgfI *
March 28, 2012 13:30 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3607 278
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2863 3140 1937
Size: 926 277 4594
Fragments arranged by size:
4594 926 277
Acc65I G'GTAC_C
Cuts at: 3118 3118
Size: 5797
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2796 3860 5244 5512 1632
Size: 1164 1064 1384 268 1917
Fragments arranged by size:
1917 1384 1164 1064 268
AflII C'TTAA_G
Cuts at: 820 1017 2026 820
Size: 197 1009 4591
Fragments arranged by size:
4591 1009 197
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 2118 1166
Size: 952 4845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5797
AlwNI CAG_nnn'CTG
Cuts at: 2054 3540 5112 2054
Size: 1486 1572 2739
Fragments arranged by size:
2739 1572 1486
ApaLI G'TGCA_C
Cuts at: 3854 5010 3854
Size: 1156 4641
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 1870
Size: 73 1179 4545
Fragments arranged by size:
4545 1179 73
BamHI G'GATC_C
Cuts at: 3127 3494 3127
Size: 367 5430
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3118 4573 618
Size: 325 217 627 1331 1455 1842
Fragments arranged by size:
1842 1455 1331 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3116 1391
Size: 1725 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2802 3063 928
Size: 971 903 261 3662
Fragments arranged by size:
3662 971 903 261
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 5797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3988 4022 1903
Size: 34 50 34 1967 34 3678
Fragments arranged by size:
3678 1967 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2181 4408 136
Size: 107 122 71 631 605 509 2227 1525
Fragments arranged by size:
2227 1525 631 605 509 122 107 71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 3214 3214
Size: 5797
Bme1580I G_kGCm'C
Cuts at: 1790 3858 5014 1790
Size: 2068 1156 2573
Fragments arranged by size:
2573 2068 1156
BmgBI CAC'GTC
Cuts at: 1318 2209 1318
Size: 891 4906
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3528 4486 442
Size: 416 53 83 399 24 2111 958 1753
Fragments arranged by size:
2111 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2919 4457 1239
Size: 1680 1538 2579
Fragments arranged by size:
2579 1680 1538
Bpu10I CC'TnA_GC
Cuts at: 2700 2700
Size: 5797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1998 3922 4787 5085 1998
Size: 1924 865 298 2710
Fragments arranged by size:
2710 1924 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3588 493
Size: 711 2384 2702
Fragments arranged by size:
2702 2384 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3604 3986 4621 275
Size: 53 83 186 936 2071 382 635 1451
Fragments arranged by size:
2071 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2383 2413 1178
Size: 30 165 30 980 30 4562
Fragments arranged by size:
4562 980 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5797
BseYI C'CCAG_C
Cuts at: 5000 5496 5000
Size: 496 5301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5224
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 4008 5036 1540
Size: 275 186 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 2447 3858 3943 5014 2331
Size: 116 1411 85 1071 3114
Fragments arranged by size:
3114 1411 1071 116 85
BsmI GAATG_Cn'
Cuts at: 2517 2589 3308 3401 2517
Size: 72 719 93 4913
Fragments arranged by size:
4913 719 93 72
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2447 3116 3858 3943 5014 1391
Size: 399 541 116 669 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 541 399 116 85
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 3686 5416 3686
Size: 1730 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2078 3152 844
Size: 1234 1074 3489
Fragments arranged by size:
3489 1234 1074
BsrBI CCG'CTC
Cuts at: 1394 1823 3684 1394
Size: 429 1861 3507
Fragments arranged by size:
3507 1861 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2946 4292 4466 65
Size: 1183 1698 1346 174 1396
Fragments arranged by size:
1698 1396 1346 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4441 4628 1700
Size: 110 2631 187 2869
Fragments arranged by size:
2869 2631 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5797
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 2437 4601 2437
Size: 2164 3633
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstXI CCAn_nnnn'nTGG
Cuts at: 2120 2120
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 3127 3494 3879 3896 5348 5434 5446 5792
Size: 1155 367 385 17 1452 86 12 346
Cuts at: 5792 1972
Size: 1977
Fragments arranged by size:
1977 1452 1155 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2332 3090 513
Size: 552 1267 758 3220
Fragments arranged by size:
3220 1267 758 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2726 3313 4107 4127 2726
Size: 587 794 20 4396
Fragments arranged by size:
4396 794 587 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2871 3244 4133
Size: 54 1425 50 275 18 1041 373 889
Cuts at: 4133 5762 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1041 889 373 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 1537
Size: 275 1432 4090
Fragments arranged by size:
4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4384 4758 4904 387
Size: 1064 138 2795 374 146 1280
Fragments arranged by size:
2795 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2796 2919 3860 4457 5244 5512 1239
Size: 393 1164 123 941 597 787 268 1524
Fragments arranged by size:
1524 1164 941 787 597 393 268 123
EcoO109I rG'GnC_Cy
Cuts at: 2248 2248
Size: 5797
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 5797
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 4944 1678
Size: 3266 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373
Size: 32 135 32 2866 32 42 32 2626
Fragments arranged by size:
2866 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3141 3387 669
Size: 988 281 1203 246 3079
Fragments arranged by size:
3079 1203 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3018 748
Size: 2270 3527
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KpnI G_GTAC'C
Cuts at: 3122 3122
Size: 5797
MfeI C'AATT_G
Cuts at: 2403 3396 2403
Size: 993 4804
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2444 2480 2513 2528 2546 2600 2618 4911
Size: 1139 36 33 15 18 54 18 2293
Cuts at: 4911 5095 1305
Size: 184 2007
Fragments arranged by size:
2293 2007 1139 184 54 36 33 18
18 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3975
Fragments arranged by size:
3975 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 2135 2137 3755 4114 4273 518
Size: 648 952 17 2 1618 359 159 2042
Fragments arranged by size:
2042 1618 952 648 359 159 17 2
MspA1I CmG'CkG
Cuts at: 1606 2291 3213 3890 5038 5283 1606
Size: 685 922 677 1148 245 2120
Fragments arranged by size:
2120 1148 922 685 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5245
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2175 3155 4700 1132
Size: 97 946 980 1545 2229
Fragments arranged by size:
2229 1545 980 946 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2142 1108
Size: 1034 4763
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 5379 5411 3866
Size: 32 1481 32 4252
Fragments arranged by size:
4252 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2248
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2847 2879 2847
Size: 32 5765
PstI C_TGCA'G
Cuts at: 830 2733 3149 830
Size: 1903 416 3478
Fragments arranged by size:
3478 1903 416
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2291 2291
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 2055 2116 2729 3145 4280 4961 5152
Size: 219 1010 61 613 416 1135 681 191
Cuts at: 5152 826
Size: 1471
Fragments arranged by size:
1471 1135 1010 681 613 416 219 191
61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 3124 1872
Size: 1252 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2013 2026 3901 4802 5064 820
Size: 197 926 70 13 1875 901 262 1553
Fragments arranged by size:
1875 1553 926 901 262 197 70 13
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2385 3236 513
Size: 552 1320 851 3074
Fragments arranged by size:
3074 1320 851 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2325 2398 2927 3824 3841 3994 4179
Size: 1255 745 73 529 897 17 153 185
Cuts at: 4179 4624 325
Size: 445 1498
Fragments arranged by size:
1498 1255 897 745 529 445 185 153
73 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2769 4043 5755
Size: 274 80 33 51 1443 792 1274 1712
Cuts at: 5755 96
Size: 138
Fragments arranged by size:
1712 1443 1274 792 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2383 2401 2415 2433 2437 2469 5757 5789 2383
Size: 18 14 18 4 32 3288 32 2391
Fragments arranged by size:
3288 2391 32 32 18 18 14 4
Tth111I GACn'n_nGTC
Cuts at: 1316 2806 1316
Size: 1490 4307
XbaI T'CTAG_A
Cuts at: 2851 3133 2851
Size: 282 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 3122 1870
Size: 1252 4545
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3605 276
Size: 53 83 186 936 2071 2468
Fragments arranged by size:
2468 2071 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI