Restriction map for
pFN21AE4347
(Circular) (Six-base) MAPSORT of: pFN21AE4347.seq Check: 308 from: 1 to: 5794
pFN21AE4347 5794 bp
With 182 enzymes: SgfI *
March 28, 2012 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2182 2182
Size: 5794
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 3115 3115
Size: 5794
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 3857 5241 5509 1632
Size: 749 1476 1384 268 1917
Fragments arranged by size:
1917 1476 1384 749 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5597
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 2151 1166
Size: 985 4809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3068 1184
Size: 1884 3910
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2982 3014 2982
Size: 32 5762
AlwNI CAG_nnn'CTG
Cuts at: 3537 5109 3537
Size: 1572 4222
ApaI G_GGCC'C
Cuts at: 2619 2619
Size: 5794
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2705 3103 3329 3481 5590 1493
Size: 1212 398 226 152 2109 1697
Fragments arranged by size:
2109 1697 1212 398 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 3119 1870
Size: 73 1176 4545
Fragments arranged by size:
4545 1176 73
AvrII C'CTAG_G
Cuts at: 2200 2200
Size: 5794
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2909 3035 3115 4570
Size: 325 217 627 383 739 126 80 1455
Cuts at: 4570 618
Size: 1842
Fragments arranged by size:
1842 1455 739 627 383 325 217 126
80
BanII G_rGCy'C
Cuts at: 1391 2482 2619 3113 1391
Size: 1091 137 494 4072
Fragments arranged by size:
4072 1091 494 137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 928
Size: 971 201 4622
Fragments arranged by size:
4622 971 201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3985 4019 1903
Size: 34 2048 34 3678
Fragments arranged by size:
3678 2048 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3686 4896 3686
Size: 1210 4584
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5794
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5789 5789
Size: 5794
BlpI GC'TnA_GC
Cuts at: 2195 2262 3211 2195
Size: 67 949 4778
Fragments arranged by size:
4778 949 67
Bme1580I G_kGCm'C
Cuts at: 1790 2281 2619 2914 3855 5011 1790
Size: 491 338 295 941 1156 2573
Fragments arranged by size:
2573 1156 941 491 338 295
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2328 2591 2613
Size: 416 53 83 399 24 911 263 22
Cuts at: 2613 3525 4483 442
Size: 912 958 1753
Fragments arranged by size:
1753 958 912 911 416 399 263 83
53 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2246 2288 4454 1239
Size: 861 146 42 2166 2579
Fragments arranged by size:
2579 2166 861 146 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3919 4784 5082 3919
Size: 865 298 4631
Fragments arranged by size:
4631 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2039 2342 2373 2639 4457 882
Size: 617 540 303 31 266 1818 2219
Fragments arranged by size:
2219 1818 617 540 303 266 31
BsaAI yAC'GTr
Cuts at: 493 1204 3585 493
Size: 711 2381 2702
Fragments arranged by size:
2702 2381 711
BsaBI GATnn'nnATC
Cuts at: 5788 5788
Size: 5794
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2438 3601 3983 4618
Size: 53 83 186 936 905 1163 382 635
Cuts at: 4618 275
Size: 1451
Fragments arranged by size:
1451 1163 936 905 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2448 2920 4227 4625 4899 5046 5457 2448
Size: 472 1307 398 274 147 411 2785
Fragments arranged by size:
2785 1307 472 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5569
Fragments arranged by size:
5569 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2243 2565 2657 1186
Size: 916 141 322 92 4323
Fragments arranged by size:
4323 916 322 141 92
BseYI C'CCAG_C
Cuts at: 2563 3009 4997 5493 2563
Size: 446 1988 496 2864
Fragments arranged by size:
2864 1988 496 446
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2588 2801 3244 4005 5033 1540
Size: 275 186 587 213 443 761 1028 2301
Fragments arranged by size:
2301 1028 761 587 443 275 213 186
BsiHKAI G_wGCw'C
Cuts at: 2417 3855 3940 5011 2417
Size: 1438 85 1071 3200
Fragments arranged by size:
3200 1438 1071 85
BsmI GAATG_Cn'
Cuts at: 2877 3305 3398 2877
Size: 428 93 5273
Fragments arranged by size:
5273 428 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 2417 2482 2619 2914 3113 3855
Size: 399 491 136 65 137 295 199 742
Cuts at: 3855 3940 5011 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 491 399 295 199 137
136 85 65
BspEI T'CCGG_A
Cuts at: 2448 2920 5457 2448
Size: 472 2537 2785
Fragments arranged by size:
2785 2537 472
BspHI T'CATG_A
Cuts at: 3683 5413 3683
Size: 1730 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2182 2391 3149 844
Size: 1338 209 758 3489
Fragments arranged by size:
3489 1338 758 209
BsrBI CCG'CTC
Cuts at: 1394 1823 2261 3681 1394
Size: 429 438 1420 3507
Fragments arranged by size:
3507 1420 438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4289 4463 65
Size: 1183 3041 174 1396
Fragments arranged by size:
3041 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2250 4438 4625 1700
Size: 110 440 2188 187 2869
Fragments arranged by size:
2869 2188 440 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5794
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 3854 4866 3854
Size: 1012 4782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 3070 2254
Size: 816 4978
BstYI r'GATC_y
Cuts at: 1972 2232 2747 3124 3491 3876 3893 5345 5431
Size: 260 515 377 367 385 17 1452 86
Cuts at: 5431 5443 5789 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 515 385 377 367 346 260
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4303
Fragments arranged by size:
4303 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2172 2279 2961 3310 4104 4124 2172
Size: 107 682 349 794 20 3842
Fragments arranged by size:
3842 794 682 349 107 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2148 2183 529
Size: 35 1584 35 4140
Fragments arranged by size:
4140 1584 35 35
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DraIII CAC_nnn'GTG
Cuts at: 2977 2977
Size: 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 809
Size: 507 3485 1802
Fragments arranged by size:
3485 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2798 2847
Size: 54 1425 50 275 18 426 542 49
Cuts at: 2847 3241 4130 5759 8
Size: 394 889 1629 43
Fragments arranged by size:
1629 1425 889 542 426 394 275 54
50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2798 3241 1537
Size: 275 986 443 4090
Fragments arranged by size:
4090 986 443 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3724 1353
Size: 1160 1211 3423
Fragments arranged by size:
3423 1211 1160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4381 4755 4901 387
Size: 1064 138 2792 374 146 1280
Fragments arranged by size:
2792 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2246 2288 2381 3857 4454 5241
Size: 393 468 146 42 93 1476 597 787
Cuts at: 5241 5509 1239
Size: 268 1524
Fragments arranged by size:
1524 1476 787 597 468 393 268 146
93 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2240 2904 2240
Size: 664 5130
EcoO109I rG'GnC_Cy
Cuts at: 2137 2204 2308 2315 2505 3083 2137
Size: 67 104 7 190 578 4848
Fragments arranged by size:
4848 578 190 104 67 7
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 4941 1678
Size: 3263 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 2915 2947 4435
Size: 32 135 32 552 32 759 32 1488
Cuts at: 4435 4467 4509 4541 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1488 759 552 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2831 2934 3138 3384 669
Size: 988 281 893 103 204 246 3079
Fragments arranged by size:
3079 988 893 281 246 204 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 3119 3119
Size: 5794
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2535 4908 5092 1305
Size: 1230 2373 184 2007
Fragments arranged by size:
2373 2007 1230 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3972
Fragments arranged by size:
3972 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2151 3752 4111 4270 518
Size: 648 936 49 1601 359 159 2042
Fragments arranged by size:
2042 1601 936 648 359 159 49
MspA1I CmG'CkG
Cuts at: 1606 2266 2813 3210 3887 5035 5280 1606
Size: 660 547 397 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 660 547 397 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4303
Fragments arranged by size:
4303 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 3152 4697 1132
Size: 97 1923 1545 2229
Fragments arranged by size:
2229 1923 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2182 1348
Size: 834 4960
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5794
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3083 3083
Size: 5794
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 2615 2615
Size: 5794
PstI C_TGCA'G
Cuts at: 830 2286 2968 3057 3146 830
Size: 1456 682 89 89 3478
Fragments arranged by size:
3478 1456 682 89 89
PvuI CG_AT'CG
Cuts at: 2001 2588 2001
Size: 587 5207
PvuII CAG'CTG
Cuts at: 2266 2813 2266
Size: 547 5247
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 2286 3146 2286
Size: 860 4934
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2282 2964 3053 3142 4277 4958 5149
Size: 219 1237 682 89 89 1135 681 191
Cuts at: 5149 826
Size: 1471
Fragments arranged by size:
1471 1237 1135 682 681 219 191 89
89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3898 4799 5061 820
Size: 197 926 1955 901 262 1553
Fragments arranged by size:
1955 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 2860 1517
Size: 1343 4451
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2200 3233 513
Size: 552 939 45 151 1033 3074
Fragments arranged by size:
3074 1033 939 552 151 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 2749 3023 3821 3838 3991 4176
Size: 1255 965 204 274 798 17 153 185
Cuts at: 4176 4621 325
Size: 445 1498
Fragments arranged by size:
1498 1255 965 798 445 274 204 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4040 5752 96
Size: 274 80 33 51 1443 2063 1712 138
Fragments arranged by size:
2063 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2961 2993 5754 5786 2961
Size: 32 2761 32 2969
Fragments arranged by size:
2969 2761 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5794
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5794
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 3923 3923
Size: 5794
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI