Restriction map for
pFN21AE4343
(Circular) (Six-base) MAPSORT of: pFN21AE4343.seq Check: 6633 from: 1 to: 5767
pFN21AE4343 5767 bp
With 182 enzymes: SgfI *
March 28, 2012 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2370 2370
Size: 5767
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2859 3110 1937
Size: 922 251 4594
Fragments arranged by size:
4594 922 251
Acc65I G'GTAC_C
Cuts at: 3088 3088
Size: 5767
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2548 3830 5214 5482 1632
Size: 916 1282 1384 268 1917
Fragments arranged by size:
1917 1384 1282 916 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 2578 4666 5643 1203
Size: 1375 2088 977 1327
Fragments arranged by size:
2088 1375 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5767
AlwNI CAG_nnn'CTG
Cuts at: 2755 3510 5082 2755
Size: 755 1572 3440
Fragments arranged by size:
3440 1572 755
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 2736 3076 3302 3454 5563 1493
Size: 1243 340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1243 340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2611 3092 1870
Size: 73 282 386 481 4545
Fragments arranged by size:
4545 481 386 282 73
BamHI G'GATC_C
Cuts at: 3097 3464 3097
Size: 367 5400
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3088 4543 618
Size: 325 217 627 1301 1455 1842
Fragments arranged by size:
1842 1455 1301 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2537 3086 1391
Size: 1146 549 4072
Fragments arranged by size:
4072 1146 549
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3958 3992 1903
Size: 34 50 34 1937 34 3678
Fragments arranged by size:
3678 1937 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 2814 1548
Size: 1266 4501
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4378 136
Size: 107 122 71 631 605 2706 1525
Fragments arranged by size:
2706 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2559 5762 2559
Size: 3203 2564
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 3828 4984 1790
Size: 2038 1156 2573
Fragments arranged by size:
2573 2038 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2075 2377 3498
Size: 416 53 83 399 24 658 302 1121
Cuts at: 3498 4456 442
Size: 958 1753
Fragments arranged by size:
1753 1121 958 658 416 399 302 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2315 4427 1239
Size: 944 132 2112 2579
Fragments arranged by size:
2579 2112 944 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3558 493
Size: 711 2354 2702
Fragments arranged by size:
2702 2354 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 2472 3574 3956
Size: 53 83 186 936 675 264 1102 382
Cuts at: 3956 4591 275
Size: 635 1451
Fragments arranged by size:
1451 1102 936 675 635 382 264 186
83 53
BsaWI w'CCGG_w
Cuts at: 2638 4200 4598 4872 5019 5430 2638
Size: 1562 398 274 147 411 2975
Fragments arranged by size:
2975 1562 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5767
BseYI C'CCAG_C
Cuts at: 4970 5466 4970
Size: 496 5271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2006 2168 2342 2348 2420 2764 1163
Size: 573 270 162 174 6 72 344 4166
Fragments arranged by size:
4166 573 344 270 174 162 72 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3217 3978 5006 1540
Size: 275 186 33 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 2040 2403 2709 3828 3913 4984 2040
Size: 363 306 1119 85 1071 2823
Fragments arranged by size:
2823 1119 1071 363 306 85
BsmI GAATG_Cn'
Cuts at: 2603 2940 3278 3371 2603
Size: 337 338 93 4999
Fragments arranged by size:
4999 338 337 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2403 2537 2709 3086 3828 3913
Size: 399 250 363 134 172 377 742 85
Cuts at: 3913 4984 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 377 363 250 172
134 85
BspEI T'CCGG_A
Cuts at: 2638 5430 2638
Size: 2792 2975
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 2310 2370 2821 3122 844
Size: 1346 120 60 451 301 3489
Fragments arranged by size:
3489 1346 451 301 120 60
BsrBI CCG'CTC
Cuts at: 1394 1823 3654 1394
Size: 429 1831 3507
Fragments arranged by size:
3507 1831 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4262 4436 65
Size: 1183 3014 174 1396
Fragments arranged by size:
3014 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4411 4598 1700
Size: 110 2601 187 2869
Fragments arranged by size:
2869 2601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5767
BssSI C'ACGA_G
Cuts at: 3827 4839 3827
Size: 1012 4755
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 3046 3046
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2559 3097 3464 3849 3866 5318 5404 5416
Size: 587 538 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 587 538 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2860 2860
Size: 5767
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 2478 3283 4077 4097 2478
Size: 805 794 20 4148
Fragments arranged by size:
4148 805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4774 809
Size: 507 3458 1802
Fragments arranged by size:
3458 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2281 3214
Size: 54 1425 50 275 18 201 250 933
Cuts at: 3214 4103 5732 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 933 889 275 250 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3214 1537
Size: 275 219 1183 4090
Fragments arranged by size:
4090 1183 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 2987 3697 1353
Size: 1269 365 710 3423
Fragments arranged by size:
3423 1269 710 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2315 2548 3830 4427 5214 5482
Size: 393 551 132 233 1282 597 787 268
Cuts at: 5482 1239
Size: 1524
Fragments arranged by size:
1524 1282 787 597 551 393 268 233
132
EcoO109I rG'GnC_Cy
Cuts at: 2158 2326 2673 2158
Size: 168 347 5252
Fragments arranged by size:
5252 347 168
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2651 2651
Size: 5767
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 4914 1678
Size: 3236 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2551 2583 2799 2831 4408
Size: 32 135 32 979 32 216 32 1577
Cuts at: 4408 4440 4482 4514 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1577 979 216 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KpnI G_GTAC'C
Cuts at: 3092 3092
Size: 5767
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4881 5065 1305
Size: 3576 184 2007
Fragments arranged by size:
3576 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2283 10
Size: 54 1425 343 451 3494
Fragments arranged by size:
3494 1425 451 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2786 3044 3725 4084 4243 518
Size: 648 1362 258 258 681 359 159 2042
Fragments arranged by size:
2042 1362 681 648 359 258 258 159
MspA1I CmG'CkG
Cuts at: 1606 2356 2412 2651 2752 3183 3860 5008 5253
Size: 750 56 239 101 431 677 1148 245
Cuts at: 5253 1606
Size: 2120
Fragments arranged by size:
2120 1148 750 677 431 245 239 101
56
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5215
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 2317 2582 3125 4670 1132
Size: 97 1088 265 543 1545 2229
Fragments arranged by size:
2229 1545 1088 543 265 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 2578 4666 2578
Size: 2088 3679
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5767
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2323 3836 3868 5349 5381 2291
Size: 32 1513 32 1481 32 2677
Fragments arranged by size:
2677 1513 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2673 2673
Size: 5767
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 2451 3337 2451
Size: 886 4881
PspXI vC'TCGA_Gb
Cuts at: 2225 2225
Size: 5767
PstI C_TGCA'G
Cuts at: 830 2169 3119 830
Size: 1339 950 3478
Fragments arranged by size:
3478 1339 950
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5767
PvuII CAG'CTG
Cuts at: 2356 2752 2356
Size: 396 5371
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SanDI GG'GwC_CC
Cuts at: 2673 2673
Size: 5767
SapI GCTCTTCn'nnn_
Cuts at: 2622 2622
Size: 5767
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 4015 5727 4015
Size: 1712 4055
SfcI C'TryA_G
Cuts at: 826 1045 2165 2341 3115 4250 4931 5122 826
Size: 219 1120 176 774 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 774 681 219 191 176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2613 3094 1872
Size: 741 481 4545
Fragments arranged by size:
4545 741 481
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2225 3871 4772 5034 820
Size: 197 926 282 1646 901 262 1553
Fragments arranged by size:
1646 1553 926 901 282 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 2657 3691 5
Size: 47 2605 1034 2081
Fragments arranged by size:
2605 2081 1034 47
StuI AGG'CCT
Cuts at: 1517 2502 1517
Size: 985 4782
StyI C'CwwG_G
Cuts at: 513 1065 2233 3206 513
Size: 552 1168 973 3074
Fragments arranged by size:
3074 1168 973 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3794 3811 3964 4149 4594 325
Size: 1255 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2586 2775 2888
Size: 274 80 33 51 1443 609 189 113
Cuts at: 2888 4013 5725 96
Size: 1125 1712 138
Fragments arranged by size:
1712 1443 1125 609 274 189 138 113
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 5735
Tth111I GACn'n_nGTC
Cuts at: 1316 2904 1316
Size: 1588 4179
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 2225 1943
Size: 282 5485
XmaI C'CCGG_G
Cuts at: 1870 2611 3092 1870
Size: 741 481 4545
Fragments arranged by size:
4545 741 481
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI
SacII SexAI SfiI SfoI SrfI SwaI XcmI