Restriction map for
pFN21AE4339
(Circular) (Six-base) MAPSORT of: pFN21AE4339.seq Check: 6466 from: 1 to: 5758
pFN21AE4339 5758 bp
With 182 enzymes: SgfI *
March 28, 2012 13:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 2898 3821 5205 5473 1632
Size: 458 808 923 1384 268 1917
Fragments arranged by size:
1917 1384 923 808 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2264 1184
Size: 1080 4678
AlwNI CAG_nnn'CTG
Cuts at: 2470 3501 5073 2470
Size: 1031 1572 3155
Fragments arranged by size:
3155 1572 1031
ApaI G_GGCC'C
Cuts at: 2737 2737
Size: 5758
ApaLI G'TGCA_C
Cuts at: 2808 3815 4971 2808
Size: 1007 1156 3595
Fragments arranged by size:
3595 1156 1007
ApoI r'AATT_y
Cuts at: 1493 2561 2620 2995 3067 3293 3445 5554 1493
Size: 1068 59 375 72 226 152 2109 1697
Fragments arranged by size:
2109 1697 1068 375 226 152 72 59
AseI AT'TA_AT
Cuts at: 160 1032 2415 4312 160
Size: 872 1383 1897 1606
Fragments arranged by size:
1897 1606 1383 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 3083 1870
Size: 73 166 974 4545
Fragments arranged by size:
4545 974 166 73
AvrII C'CTAG_G
Cuts at: 2119 2119
Size: 5758
BamHI G'GATC_C
Cuts at: 2012 3088 3455 2012
Size: 1076 367 4315
Fragments arranged by size:
4315 1076 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 4534 618
Size: 325 217 627 1292 1455 1842
Fragments arranged by size:
1842 1455 1292 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2737 2964 3077 1391
Size: 639 707 227 113 4072
Fragments arranged by size:
4072 707 639 227 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2720 2856 928
Size: 971 821 136 3830
Fragments arranged by size:
3830 971 821 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3949 3983 1903
Size: 34 50 34 1928 34 3678
Fragments arranged by size:
3678 1928 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 2314 2496 2700 3046 1548
Size: 766 182 204 346 4260
Fragments arranged by size:
4260 766 346 204 182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 4369 136
Size: 107 122 71 631 605 1060 1637 1525
Fragments arranged by size:
1637 1525 1060 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 2217 3175 2217
Size: 958 4800
Bme1580I G_kGCm'C
Cuts at: 1790 2737 2812 3819 4975 1790
Size: 947 75 1007 1156 2573
Fragments arranged by size:
2573 1156 1007 947 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2375 2492 2933 4418 1239
Size: 937 199 117 441 1485 2579
Fragments arranged by size:
2579 1485 937 441 199 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2246 3883 4748 5046 2246
Size: 1637 865 298 2958
Fragments arranged by size:
2958 1637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2057 2108 2222 4421 882
Size: 617 558 51 114 2199 2219
Fragments arranged by size:
2219 2199 617 558 114 51
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2348 4191 4589 4863 5010 5421 2348
Size: 1843 398 274 147 411 2685
Fragments arranged by size:
2685 1843 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 1178
Size: 30 165 30 1065 30 4438
Fragments arranged by size:
4438 1065 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 1186
Size: 1105 4653
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 1163
Size: 573 579 4606
Fragments arranged by size:
4606 579 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2214 3208 3969 4997 1540
Size: 275 186 213 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 275 213 186
BsiHKAI G_wGCw'C
Cuts at: 2262 2478 2590 2812 2964 3819 3904 4975 2262
Size: 216 112 222 152 855 85 1071 3045
Fragments arranged by size:
3045 1071 855 222 216 152 112 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2262 2478 2590 2737 2812 2964
Size: 399 240 232 216 112 147 75 152
Cuts at: 2964 3077 3819 3904 4975 1391
Size: 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 240 232 216 152
147 113 112 85 75
BspEI T'CCGG_A
Cuts at: 2348 5421 2348
Size: 3073 2685
BspHI T'CATG_A
Cuts at: 2499 2605 2652 3647 5377 2499
Size: 106 47 995 1730 2880
Fragments arranged by size:
2880 1730 995 106 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2624 3113 844
Size: 1780 489 3489
Fragments arranged by size:
3489 1780 489
BsrBI CCG'CTC
Cuts at: 1394 1823 2216 3645 1394
Size: 429 393 1429 3507
Fragments arranged by size:
3507 1429 429 393
BsrDI GCAATG_nn'
Cuts at: 65 1248 2454 2929 4253 4427 65
Size: 1183 1206 475 1324 174 1396
Fragments arranged by size:
1396 1324 1206 1183 475 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2585 794
Size: 1791 3967
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2012 3088 3455 3840 3857 5309 5395 5407
Size: 40 1076 367 385 17 1452 86 12
Cuts at: 5407 5753 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1076 385 367 346 86 40
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2235 2293 513
Size: 552 1170 58 3978
Fragments arranged by size:
3978 1170 552 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2971 3006 529
Size: 35 2407 35 3281
Fragments arranged by size:
3281 2407 35 35
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2724 3205
Size: 54 1425 50 275 18 381 513 481
Cuts at: 3205 4094 5723 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 513 481 381 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2211 3205 1537
Size: 275 399 994 4090
Fragments arranged by size:
4090 994 399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3688 1353
Size: 2335 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2962 2962
Size: 5758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2176 2375 2492 2898 2933 3821
Size: 393 458 86 199 117 406 35 888
Cuts at: 3821 4418 5205 5473 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 458 406 393 268
199 117 86 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2540 2360
Size: 180 5578
EcoO109I rG'GnC_Cy
Cuts at: 2733 2734 2733
Size: 1 5757
EcoRI G'AATT_C
Cuts at: 2995 3067 3445 2995
Size: 72 378 5308
Fragments arranged by size:
5308 378 72
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 2740 2772 4399
Size: 32 135 32 628 32 508 32 1627
Cuts at: 4399 4431 4473 4505 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1627 628 508 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2343 2692 3102 3348 669
Size: 988 281 405 349 410 246 3079
Fragments arranged by size:
3079 988 410 405 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 3006 4872 5056 1305
Size: 1116 585 1866 184 2007
Fragments arranged by size:
2007 1866 1116 585 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2726 10
Size: 54 1425 343 894 3042
Fragments arranged by size:
3042 1425 894 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2487 3042 3716 4075 4234 518
Size: 648 1321 555 674 359 159 2042
Fragments arranged by size:
2042 1321 674 648 555 359 159
MspA1I CmG'CkG
Cuts at: 1606 2154 3174 3851 4999 5244 1606
Size: 548 1020 677 1148 245 2120
Fragments arranged by size:
2120 1148 1020 677 548 245
NcoI C'CATG_G
Cuts at: 513 1065 2293 513
Size: 552 1228 3978
Fragments arranged by size:
3978 1228 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2470 1108
Size: 1362 4396
PfoI T'CCnGG_A
Cuts at: 2271 2271
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2746 2778 3827 3859 5340 5372 2746
Size: 32 1049 32 1481 32 3132
Fragments arranged by size:
3132 1481 1049 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2648 1613
Size: 1035 4723
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2733 2733
Size: 5758
PstI C_TGCA'G
Cuts at: 830 3110 830
Size: 2280 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SacI G_AGCT'C
Cuts at: 2964 2964
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 3106 4241 4922 5113 826
Size: 219 2061 1135 681 191 1471
Fragments arranged by size:
2061 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2732 2732
Size: 5758
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 3862 4763 5025 820
Size: 197 926 318 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 318 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 2355 2531 1517
Size: 838 176 4744
Fragments arranged by size:
4744 838 176
StyI C'CwwG_G
Cuts at: 513 1065 2119 2293 3197 513
Size: 552 1054 174 904 3074
Fragments arranged by size:
3074 1054 904 552 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4004 5716 96
Size: 274 80 33 51 1443 2027 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2468 2500 5718 5750 2468
Size: 32 3218 32 2476
Fragments arranged by size:
3218 2476 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI
XcmI