Restriction map for
pFN21AE4338
(Circular) (Six-base) MAPSORT of: pFN21AE4338.seq Check: 6611 from: 1 to: 5755
pFN21AE4338 5755 bp
With 182 enzymes: SgfI *
March 1, 2012 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2513 2513
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3012 3818 5202 5470 1632
Size: 1380 806 1384 268 1917
Fragments arranged by size:
1917 1384 1380 806 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 2381 4484 2381
Size: 2103 3652
AleI CACnn'nnGTG
Cuts at: 1166 2408 1166
Size: 1242 4513
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2720 1184
Size: 1536 4219
AlwNI CAG_nnn'CTG
Cuts at: 2965 3498 5070 2965
Size: 533 1572 3650
Fragments arranged by size:
3650 1572 533
ApaI G_GGCC'C
Cuts at: 2469 2469
Size: 5755
ApaLI G'TGCA_C
Cuts at: 2336 2695 3812 4968 2336
Size: 359 1117 1156 3123
Fragments arranged by size:
3123 1156 1117 359
ApoI r'AATT_y
Cuts at: 1493 2346 3064 3290 3442 5551 1493
Size: 853 718 226 152 2109 1697
Fragments arranged by size:
2109 1697 853 718 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2541 3080 1870
Size: 73 598 539 4545
Fragments arranged by size:
4545 598 539 73
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2469 3074 1391
Size: 1078 605 4072
Fragments arranged by size:
4072 1078 605
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3946 3980 1903
Size: 34 50 34 1925 34 3678
Fragments arranged by size:
3678 1925 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2340 2469 2699 3816 4972 1790
Size: 550 129 230 1117 1156 2573
Fragments arranged by size:
2573 1156 1117 550 230 129
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2079 2987 2996 4415 1239
Size: 840 908 9 1419 2579
Fragments arranged by size:
2579 1419 908 840 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2288 2556 4418 882
Size: 617 789 268 1862 2219
Fragments arranged by size:
2219 1862 789 617 268
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4188 4586 4860 5007 5418 4188
Size: 398 274 147 411 4525
Fragments arranged by size:
4525 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2077 2107 1178
Size: 30 165 30 674 30 4826
Fragments arranged by size:
4826 674 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 2807 2963 2995 1186
Size: 961 660 156 32 3946
Fragments arranged by size:
3946 961 660 156 32
BseYI C'CCAG_C
Cuts at: 2279 2468 3004 3037 4958 5454 2279
Size: 189 536 33 1921 496 2580
Fragments arranged by size:
2580 1921 536 496 189 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2521 2572 3015 1163
Size: 573 785 51 443 3903
Fragments arranged by size:
3903 785 573 443 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2699 3816 3901 4972 2340
Size: 359 1117 85 1071 3123
Fragments arranged by size:
3123 1117 1071 359 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
BsmBI CGTCTCn'nnnn_
Cuts at: 2409 2409
Size: 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2469 2699 3074 3816 3901 4972
Size: 399 550 129 230 375 742 85 1071
Cuts at: 4972 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 550 399 375 230 129
85
BspEI T'CCGG_A
Cuts at: 5418 5418
Size: 5755
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2513 3110 844
Size: 1669 597 3489
Fragments arranged by size:
3489 1669 597
BsrBI CCG'CTC
Cuts at: 1394 1823 2075 3642 1394
Size: 429 252 1567 3507
Fragments arranged by size:
3507 1567 429 252
BsrDI GCAATG_nn'
Cuts at: 65 1248 2620 4250 4424 65
Size: 1183 1372 1630 174 1396
Fragments arranged by size:
1630 1396 1372 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2742 4399 4586 1700
Size: 110 932 1657 187 2869
Fragments arranged by size:
2869 1657 932 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5755
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 2438 3815 4827 2438
Size: 1377 1012 3366
Fragments arranged by size:
3366 1377 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2197 2478 794
Size: 1403 281 4071
Fragments arranged by size:
4071 1403 281
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2895 3085 3452 3837 3854 5306 5392 5404
Size: 923 190 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 923 385 367 346 190 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2058 2709 513
Size: 552 993 651 3559
Fragments arranged by size:
3559 993 651 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2584 3032 3271 4065 4085 2584
Size: 448 239 794 20 4254
Fragments arranged by size:
4254 794 448 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 3202 4091
Size: 54 1425 50 275 18 768 604 889
Cuts at: 4091 5720 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 768 604 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2208 2988 3042 3685 1353
Size: 855 780 54 643 3423
Fragments arranged by size:
3423 855 780 643 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2987 2996 3012 3818 4415 5202
Size: 393 447 908 9 16 806 597 787
Cuts at: 5202 5470 1239
Size: 268 1524
Fragments arranged by size:
1524 908 806 787 597 447 393 268
16 9
EcoO109I rG'GnC_Cy
Cuts at: 2914 2971 2914
Size: 57 5698
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 5755
FspI TGC'GCA
Cuts at: 2201 4261 2201
Size: 2060 3695
HaeII r_GCGC'y
Cuts at: 1678 3005 4902 1678
Size: 1327 1897 2531
Fragments arranged by size:
2531 1897 1327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2077 2109 2367 2399 4396
Size: 32 135 32 505 32 258 32 1997
Cuts at: 4396 4428 4470 4502 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1997 505 258 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3099 3345 669
Size: 988 281 1161 246 3079
Fragments arranged by size:
3079 1161 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2102 2296 4869 5053 1305
Size: 797 194 2573 184 2007
Fragments arranged by size:
2573 2007 797 194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 10
Size: 54 1425 343 768 3165
Fragments arranged by size:
3165 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2408 2594 3713 4072 4231 518
Size: 648 1167 75 186 1119 359 159 2042
Fragments arranged by size:
2042 1167 1119 648 359 186 159 75
MspA1I CmG'CkG
Cuts at: 1606 2011 2569 3029 3171 3848 4996 5241 1606
Size: 405 558 460 142 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 460 405 245 142
NaeI GCC'GGC
Cuts at: 2744 2744
Size: 5755
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5203
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NgoMIV G'CCGG_C
Cuts at: 2742 2742
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2476 3113 4658 1132
Size: 97 1247 637 1545 2229
Fragments arranged by size:
2229 1545 1247 637 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5755
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2842 2965 1108
Size: 1734 123 3898
Fragments arranged by size:
3898 1734 123
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2914 2971 2914
Size: 57 5698
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2465 2465
Size: 5755
PstI C_TGCA'G
Cuts at: 830 3107 830
Size: 2277 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2569 2569
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SfcI C'TryA_G
Cuts at: 826 1045 3103 4238 4919 5110 826
Size: 219 2058 1135 681 191 1471
Fragments arranged by size:
2058 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 2543 3082 1872
Size: 671 539 4545
Fragments arranged by size:
4545 671 539
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 2679 1517
Size: 1162 4593
StyI C'CwwG_G
Cuts at: 513 1065 2787 3194 513
Size: 552 1722 407 3074
Fragments arranged by size:
3074 1722 552 407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2808 3782 3799 3952 4137 4582 325
Size: 1255 1228 974 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1228 974 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 2499 4001
Size: 274 80 33 51 1443 498 24 1502
Cuts at: 4001 5713 96
Size: 1712 138
Fragments arranged by size:
1712 1502 1443 498 274 138 80 51
33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2537 2569 2611 2643 5715 5747 2537
Size: 32 42 32 3072 32 2545
Fragments arranged by size:
3072 2545 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2746 2746
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 2541 3080 1870
Size: 671 539 4545
Fragments arranged by size:
4545 671 539
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI