Restriction map for
pFN21AE4337
(Circular) (Six-base) MAPSORT of: pFN21AE4337.seq Check: 1312 from: 1 to: 5749
pFN21AE4337 5749 bp
With 182 enzymes: SgfI *
March 28, 2012 13:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3012 3812 5196 5464 1632
Size: 1380 800 1384 268 1917
Fragments arranged by size:
1917 1384 1380 800 268
AflII C'TTAA_G
Cuts at: 820 1017 2767 820
Size: 197 1750 3802
Fragments arranged by size:
3802 1750 197
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2167 2350 2405 2428 3492 5064 2167
Size: 183 55 23 1064 1572 2852
Fragments arranged by size:
2852 1572 1064 183 55 23
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 2602 3079 3446 2602
Size: 477 367 4905
Fragments arranged by size:
4905 477 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2573 3070 4525 618
Size: 325 217 627 786 497 1455 1842
Fragments arranged by size:
1842 1455 786 627 497 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2221 2277 3068 1391
Size: 744 86 56 791 4072
Fragments arranged by size:
4072 791 744 86 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 2398 3166 2398
Size: 768 4981
Bme1580I G_kGCm'C
Cuts at: 1790 2578 2816 2822 3810 4966 1790
Size: 788 238 6 988 1156 2573
Fragments arranged by size:
2573 1156 988 788 238 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2416 3480 4438
Size: 416 53 83 399 24 999 1064 958
Cuts at: 4438 442
Size: 1753
Fragments arranged by size:
1753 1064 999 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2093 2372 4409 1239
Size: 845 9 279 2037 2579
Fragments arranged by size:
2579 2037 845 279 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 3874 4739 5037 2261
Size: 1613 865 298 2973
Fragments arranged by size:
2973 1613 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2945 2975 1178
Size: 30 165 30 1542 30 3952
Fragments arranged by size:
3952 1542 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2966 1186
Size: 1021 759 3969
Fragments arranged by size:
3969 1021 759
BseYI C'CCAG_C
Cuts at: 2936 2956 4952 5448 2936
Size: 20 1996 496 3237
Fragments arranged by size:
3237 1996 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 1163
Size: 573 582 4594
Fragments arranged by size:
4594 582 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2655 3199 3960 4988 1540
Size: 275 186 654 544 761 1028 2301
Fragments arranged by size:
2301 1028 761 654 544 275 186
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
BsmBI CGTCTCn'nnnn_
Cuts at: 2234 2234
Size: 5749
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2221 2277 2578 2816 2822 3068
Size: 399 345 86 56 301 238 6 246
Cuts at: 3068 3810 3895 4966 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 345 301 246 238
86 85 56 6
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 2358 3638 5368 2358
Size: 1280 1730 2739
Fragments arranged by size:
2739 1730 1280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2436 3104 844
Size: 1592 668 3489
Fragments arranged by size:
3489 1592 668
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 3636 1394
Size: 429 355 1458 3507
Fragments arranged by size:
3507 1458 429 355
BsrDI GCAATG_nn'
Cuts at: 65 1248 2692 2757 4244 4418 65
Size: 1183 1444 65 1487 174 1396
Fragments arranged by size:
1487 1444 1396 1183 174 65
BsrFI r'CCGG_y
Cuts at: 1700 1810 4393 4580 1700
Size: 110 2583 187 2869
Fragments arranged by size:
2869 2583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2585 2585
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2602 2740 2799 2877 2998 3079 3446 3831
Size: 630 138 59 78 121 81 367 385
Cuts at: 3831 3848 5300 5386 5398 5744 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 630 385 367 346 138 121
86 81 78 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 2974 2974
Size: 5749
BtgI C'CryG_G
Cuts at: 513 1065 2004 2122 2283 2368 2451 513
Size: 552 939 118 161 85 83 3811
Fragments arranged by size:
3811 939 552 161 118 85 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2550 3265 4059 4079 2550
Size: 715 794 20 4220
Fragments arranged by size:
4220 794 715 20
ClaI AT'CG_AT
Cuts at: 1554 2100 1554
Size: 546 5203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DraIII CAC_nnn'GTG
Cuts at: 2059 2374 2059
Size: 315 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2745 3196 4085
Size: 54 1425 50 275 18 915 451 889
Cuts at: 4085 5714 8
Size: 1629 43
Fragments arranged by size:
1629 1425 915 889 451 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2237 3679 1353
Size: 884 1442 3423
Fragments arranged by size:
3423 1442 884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2093 2372 3012 3812 4409 5196
Size: 393 452 9 279 640 800 597 787
Cuts at: 5196 5464 1239
Size: 268 1524
Fragments arranged by size:
1524 800 787 640 597 452 393 279
268 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2438 2519 2438
Size: 81 5668
EcoO109I rG'GnC_Cy
Cuts at: 2512 2668 2512
Size: 156 5593
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 5749
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 3010 4896 1678
Size: 1332 1886 2531
Fragments arranged by size:
2531 1886 1332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373
Size: 32 135 32 2818 32 42 32 2626
Fragments arranged by size:
2818 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2326 3093 3339 669
Size: 988 281 388 767 246 3079
Fragments arranged by size:
3079 988 767 388 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 2326 3339 2326
Size: 1013 4736
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 10
Size: 54 1425 343 915 3012
Fragments arranged by size:
3012 1425 915 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3707 4066 4225 518
Size: 648 2541 359 159 2042
Fragments arranged by size:
2541 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3165 3842 4990 5235 1606
Size: 1559 677 1148 245 2120
Fragments arranged by size:
2120 1559 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4258
Fragments arranged by size:
4258 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 3107 4652 1132
Size: 97 1878 1545 2229
Fragments arranged by size:
2229 1878 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2608 1348
Size: 1260 4489
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2350 2737 2854 1108
Size: 1242 387 117 4003
Fragments arranged by size:
4003 1242 387 117
PfoI T'CCnGG_A
Cuts at: 2160 2508 2718 2160
Size: 348 210 5191
Fragments arranged by size:
5191 348 210
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2512 2512
Size: 5749
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2408 2440 2408
Size: 32 5717
PstI C_TGCA'G
Cuts at: 830 2841 3101 830
Size: 2011 260 3478
Fragments arranged by size:
3478 2011 260
PvuI CG_AT'CG
Cuts at: 2001 2655 2001
Size: 654 5095
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SexAI A'CCwGG_T
Cuts at: 2382 2382
Size: 5749
SfcI C'TryA_G
Cuts at: 826 1045 2222 2837 3097 4232 4913 5104 826
Size: 219 1177 615 260 1135 681 191 1471
Fragments arranged by size:
1471 1177 1135 681 615 260 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2767 3853 4754 5016 820
Size: 197 926 333 491 1086 901 262 1553
Fragments arranged by size:
1553 1086 926 901 491 333 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 2864 1517
Size: 1347 4402
StyI C'CwwG_G
Cuts at: 513 1065 2004 2748 3188 513
Size: 552 939 744 440 3074
Fragments arranged by size:
3074 939 744 552 440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 3995 5707
Size: 274 80 33 51 1443 264 1754 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1754 1712 1443 274 264 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2975 2977 3007 5709 5741 2945
Size: 30 2 30 2702 32 2953
Fragments arranged by size:
2953 2702 32 30 30 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2347 2453 2938 2347
Size: 106 485 5158
Fragments arranged by size:
5158 485 106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI