Restriction map for
pFN21AE4334
(Circular) (Six-base) MAPSORT of: pFN21AE4334.seq Check: 107 from: 1 to: 5737
pFN21AE4334 5737 bp
With 182 enzymes: SgfI *
March 28, 2012 13:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3080 1937
Size: 1143 4594
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3800 5184 5452 1632
Size: 2168 1384 268 1917
Fragments arranged by size:
2168 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 2285 2537 4636 5613 1203
Size: 1082 252 2099 977 1327
Fragments arranged by size:
2099 1327 1082 977 252
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 2836 4466 2836
Size: 1630 4107
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5737
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2460 2492 2460
Size: 32 5705
AlwNI CAG_nnn'CTG
Cuts at: 2137 2600 2655 3480 5052 2137
Size: 463 55 825 1572 2822
Fragments arranged by size:
2822 1572 825 463 55
ApaLI G'TGCA_C
Cuts at: 2433 2752 3794 4950 2433
Size: 319 1042 1156 3220
Fragments arranged by size:
3220 1156 1042 319
ApoI r'AATT_y
Cuts at: 1493 3046 3272 3424 5533 1493
Size: 1553 226 152 2109 1697
Fragments arranged by size:
2109 1697 1553 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 3062 1870
Size: 73 1119 4545
Fragments arranged by size:
4545 1119 73
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2255 3058 4513 618
Size: 325 217 627 232 236 803 1455 1842
Fragments arranged by size:
1842 1455 803 627 325 236 232 217
BanII G_rGCy'C
Cuts at: 1391 3056 1391
Size: 1665 4072
BbeI G_GCGC'C
Cuts at: 2259 2259
Size: 5737
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2973 3000 928
Size: 971 1074 27 3665
Fragments arranged by size:
3665 1074 971 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 1903
Size: 34 1991 34 3678
Fragments arranged by size:
3678 1991 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 4839 3629
Size: 1210 4527
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2032 2685 4348
Size: 107 122 71 631 605 360 653 1663
Cuts at: 4348 136
Size: 1525
Fragments arranged by size:
1663 1525 653 631 605 360 122 107
71
BglII A'GATC_T
Cuts at: 2690 5732 2690
Size: 3042 2695
BlpI GC'TnA_GC
Cuts at: 2186 3154 2186
Size: 968 4769
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2437 2756 2828 3798 4954 1790
Size: 296 351 319 72 970 1156 2573
Fragments arranged by size:
2573 1156 970 351 319 296 72
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 4843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2632 4397 1239
Size: 1393 1765 2579
Fragments arranged by size:
2579 1765 1393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3862 4727 5025 3862
Size: 865 298 4574
Fragments arranged by size:
4574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4400 882
Size: 617 2901 2219
Fragments arranged by size:
2901 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 5731 5731
Size: 5737
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 3544 3926 4561
Size: 53 83 186 936 723 1288 382 635
Cuts at: 4561 275
Size: 1451
Fragments arranged by size:
1451 1288 936 723 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2942 4170 4568 4842 4989 5400 2942
Size: 1228 398 274 147 411 3279
Fragments arranged by size:
3279 1228 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5512
Fragments arranged by size:
5512 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2219 2902 2996 1186
Size: 991 42 683 94 3927
Fragments arranged by size:
3927 991 683 94 42
BseYI C'CCAG_C
Cuts at: 2090 2683 4940 5436 2090
Size: 593 2257 496 2391
Fragments arranged by size:
2391 2257 593 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5164
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2125 2182 3187 3948 4976 1540
Size: 275 186 124 57 1005 761 1028 2301
Fragments arranged by size:
2301 1028 1005 761 275 186 124 57
BsiHKAI G_wGCw'C
Cuts at: 2192 2437 2756 3798 3883 4954 2192
Size: 245 319 1042 85 1071 2975
Fragments arranged by size:
2975 1071 1042 319 245 85
BsmI GAATG_Cn'
Cuts at: 3248 3341 3248
Size: 93 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2192 2437 2756 2828 3056 3798
Size: 399 296 106 245 319 72 228 742
Cuts at: 3798 3883 4954 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 296 245 228
106 85 72
BspEI T'CCGG_A
Cuts at: 2942 5400 2942
Size: 2458 3279
BspHI T'CATG_A
Cuts at: 3626 5356 3626
Size: 1730 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2684 3092 844
Size: 1840 408 3489
Fragments arranged by size:
3489 1840 408
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2431 4232 4406 65
Size: 1183 1183 1801 174 1396
Fragments arranged by size:
1801 1396 1183 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2177 2181 2799 4381 4568 1700
Size: 110 367 4 618 1582 187 2869
Fragments arranged by size:
2869 1582 618 367 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 2139 96
Size: 2043 3694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 2194 3797 4809 2194
Size: 1603 1012 3122
Fragments arranged by size:
3122 1603 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5737
BstBI TT'CG_AA
Cuts at: 4541 4541
Size: 5737
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstXI CCAn_nnnn'nTGG
Cuts at: 2030 2542 2756 2030
Size: 512 214 5011
Fragments arranged by size:
5011 512 214
BstYI r'GATC_y
Cuts at: 1972 2690 2945 3067 3434 3819 3836 5288 5374
Size: 718 255 122 367 385 17 1452 86
Cuts at: 5374 5386 5732 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 255 122
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2914 2914
Size: 5737
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4246
Fragments arranged by size:
4246 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 2086 3253 4047 4067 2086
Size: 1167 794 20 3756
Fragments arranged by size:
3756 1167 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DraIII CAC_nnn'GTG
Cuts at: 2196 2196
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2179 3184
Size: 54 1425 50 275 18 292 57 1005
Cuts at: 3184 4073 5702 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1005 889 292 275 57 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2122 2179 3184 1537
Size: 275 310 57 1005 4090
Fragments arranged by size:
4090 1005 310 275 57
EarI CTCTTCn'nnn_
Cuts at: 1353 2234 2973 3667 1353
Size: 881 739 694 3423
Fragments arranged by size:
3423 881 739 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2632 3800 4397 5184 5452 1239
Size: 393 1000 1168 597 787 268 1524
Fragments arranged by size:
1524 1168 1000 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2666 2666
Size: 5737
EcoO109I rG'GnC_Cy
Cuts at: 2134 2134
Size: 5737
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
EcoRV GAT'ATC
Cuts at: 2746 2746
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 2259 4884 1678
Size: 581 2625 2531
Fragments arranged by size:
2625 2531 581
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2566 2598 2822 2854 4378
Size: 32 135 32 994 32 224 32 1524
Cuts at: 4378 4410 4452 4484 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1524 994 224 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KasI G'GCGC_C
Cuts at: 2255 2255
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2397 2820 4851 5035 1305
Size: 1092 423 2031 184 2007
Fragments arranged by size:
2031 2007 1092 423 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3915
Fragments arranged by size:
3915 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 3695 4054 4213 518
Size: 648 1374 1155 359 159 2042
Fragments arranged by size:
2042 1374 1155 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2127 2349 3153 3830 4978 5223 1606
Size: 521 222 804 677 1148 245 2120
Fragments arranged by size:
2120 1148 804 677 521 245 222
NaeI GCC'GGC
Cuts at: 2183 2183
Size: 5737
NarI GG'CG_CC
Cuts at: 2256 2256
Size: 5737
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4246
Fragments arranged by size:
4246 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NgoMIV G'CCGG_C
Cuts at: 2181 2181
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 2541 3095 4640 1132
Size: 97 1312 554 1545 2229
Fragments arranged by size:
2229 1545 1312 554 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5737
PciI A'CATG_T
Cuts at: 2537 4636 2537
Size: 2099 3638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2068 1108
Size: 960 4777
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2134 2134
Size: 5737
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 3307 3307
Size: 5737
PstI C_TGCA'G
Cuts at: 830 2174 3089 830
Size: 1344 915 3478
Fragments arranged by size:
3478 1344 915
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SbfI CC_TGCA'GG
Cuts at: 3089 3089
Size: 5737
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2170 3004 3085 4220 4901 5092 826
Size: 219 1125 834 81 1135 681 191 1471
Fragments arranged by size:
1471 1135 1125 834 681 219 191 81
SfoI GGC'GCC
Cuts at: 2257 2257
Size: 5737
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3841 4742 5004 820
Size: 197 926 1898 901 262 1553
Fragments arranged by size:
1898 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 3661 5
Size: 47 3609 2081
Fragments arranged by size:
3609 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5737
StyI C'CwwG_G
Cuts at: 513 1065 2004 2485 3176 513
Size: 552 939 481 691 3074
Fragments arranged by size:
3074 939 691 552 481
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2162 2216 3764 3781 3934 4119 4564
Size: 1255 582 54 1548 17 153 185 445
Cuts at: 4564 325
Size: 1498
Fragments arranged by size:
1548 1498 1255 582 445 185 153 54
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 3983 5695
Size: 274 80 33 51 1443 162 1844 1712
Cuts at: 5695 96
Size: 138
Fragments arranged by size:
1844 1712 1443 274 162 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5697 5729 5697
Size: 32 5705
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 3028 3073 3028
Size: 45 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2542 2756 2542
Size: 214 5523
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 3866 3866
Size: 5737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI