Restriction map for
pFN21AE4333
(Circular) (Six-base) MAPSORT of: pFN21AE4333.seq Check: 6748 from: 1 to: 5734
pFN21AE4333 5734 bp
With 182 enzymes: SgfI *
March 28, 2012 13:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 2754 3055 2754
Size: 301 5433
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 2923 3797 5181 5449 1632
Size: 743 548 874 1384 268 1917
Fragments arranged by size:
1917 1384 874 743 548 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 2603 4633 5610 1203
Size: 1400 2030 977 1327
Fragments arranged by size:
2030 1400 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AlwNI CAG_nnn'CTG
Cuts at: 3477 5049 3477
Size: 1572 4162
ApaI G_GGCC'C
Cuts at: 2311 2726 2797 2311
Size: 415 71 5248
Fragments arranged by size:
5248 415 71
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2576 3059 1870
Size: 73 633 483 4545
Fragments arranged by size:
4545 633 483 73
BamHI G'GATC_C
Cuts at: 2844 3064 3431 2844
Size: 220 367 5147
Fragments arranged by size:
5147 367 220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2581 2754 2881 3055 4510
Size: 325 217 627 794 173 127 174 1455
Cuts at: 4510 618
Size: 1842
Fragments arranged by size:
1842 1455 794 627 325 217 174 173
127
BanII G_rGCy'C
Cuts at: 1391 2311 2396 2726 2797 3053 1391
Size: 920 85 330 71 256 4072
Fragments arranged by size:
4072 920 330 256 85 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BbvCI CC'TCA_GC
Cuts at: 2966 2966
Size: 5734
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2140 2174 3925 3959 1903
Size: 34 203 34 1751 34 3678
Fragments arranged by size:
3678 1751 203 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 2589 1548
Size: 1041 4693
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2792 4345 136
Size: 107 122 71 631 605 1120 1553 1525
Fragments arranged by size:
1553 1525 1120 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3023 3151 3023
Size: 128 5606
Bme1580I G_kGCm'C
Cuts at: 1790 2311 2726 2797 2886 3795 4951 1790
Size: 521 415 71 89 909 1156 2573
Fragments arranged by size:
2573 1156 909 521 415 89 71
BmgBI CAC'GTC
Cuts at: 1318 2604 1318
Size: 1286 4448
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3465 4423 442
Size: 416 53 83 399 24 2048 958 1753
Fragments arranged by size:
2048 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2369 2812 2852 4394 1239
Size: 1130 443 40 1542 2579
Fragments arranged by size:
2579 1542 1130 443 40
Bpu10I CC'TnA_GC
Cuts at: 2966 2966
Size: 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2753 4397 882
Size: 617 1254 1644 2219
Fragments arranged by size:
2219 1644 1254 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2202 2337 2555 2628
Size: 53 83 186 936 669 135 218 73
Cuts at: 2628 2700 3541 3923 4558 275
Size: 72 841 382 635 1451
Fragments arranged by size:
1451 936 841 669 635 382 218 186
135 83 73 72 53
BsaWI w'CCGG_w
Cuts at: 2076 4167 4565 4839 4986 5397 2076
Size: 2091 398 274 147 411 2413
Fragments arranged by size:
2413 2091 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 1178
Size: 30 165 30 909 30 4570
Fragments arranged by size:
4570 909 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2405 2654 2999 1186
Size: 1219 249 345 3921
Fragments arranged by size:
3921 1219 345 249
BseYI C'CCAG_C
Cuts at: 2038 2267 2302 2375 2520 2775 2901 2950 4937
Size: 229 35 73 145 255 126 49 1987
Cuts at: 4937 5433 2038
Size: 496 2339
Fragments arranged by size:
2339 1987 496 255 229 145 126 73
49 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2175 2491 1163
Size: 573 439 316 4406
Fragments arranged by size:
4406 573 439 316
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2081 3184 3945 4973 1540
Size: 275 186 80 1103 761 1028 2301
Fragments arranged by size:
2301 1103 1028 761 275 186 80
BsiHKAI G_wGCw'C
Cuts at: 2277 3033 3795 3880 4951 2277
Size: 756 762 85 1071 3060
Fragments arranged by size:
3060 1071 762 756 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
BsmBI CGTCTCn'nnnn_
Cuts at: 2594 2594
Size: 5734
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2311 2396 2726 2797 2886 3033
Size: 399 487 34 85 330 71 89 147
Cuts at: 3033 3053 3795 3880 4951 1391
Size: 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 399 330 147 89
85 85 71 34 20
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2170 3089 844
Size: 1326 919 3489
Fragments arranged by size:
3489 1326 919
BsrBI CCG'CTC
Cuts at: 1394 1823 2093 3621 1394
Size: 429 270 1528 3507
Fragments arranged by size:
3507 1528 429 270
BsrDI GCAATG_nn'
Cuts at: 65 1248 2406 4229 4403 65
Size: 1183 1158 1823 174 1396
Fragments arranged by size:
1823 1396 1183 1158 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2162 4378 4565 1700
Size: 110 352 2216 187 2869
Fragments arranged by size:
2869 2216 352 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 2814 96
Size: 2520 198 3016
Fragments arranged by size:
3016 2520 198
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 2225 2874 3794 4806 2225
Size: 649 920 1012 3153
Fragments arranged by size:
3153 1012 920 649
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 794
Size: 1448 4286
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstXI CCAn_nnnn'nTGG
Cuts at: 2213 2213
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2844 3064 3431 3816 3833 5285 5371 5383
Size: 872 220 367 385 17 1452 86 12
Cuts at: 5383 5729 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 872 385 367 346 220 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2637 2637
Size: 5734
BtgI C'CryG_G
Cuts at: 513 1065 2004 3017 513
Size: 552 939 1013 3230
Fragments arranged by size:
3230 1013 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 2243 2257 2503 2564 3250 4044 4064 2158
Size: 85 14 246 61 686 794 20 3828
Fragments arranged by size:
3828 794 686 246 85 61 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2902 2937 529
Size: 35 2338 35 3326
Fragments arranged by size:
3326 2338 35 35
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2342 4741 809
Size: 507 1026 2399 1802
Fragments arranged by size:
2399 1802 1026 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 3181 4070
Size: 54 1425 50 275 18 219 1132 889
Cuts at: 4070 5699 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1132 889 275 219 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3664 1353
Size: 2311 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2375 2812 2852 2923 3797 4394
Size: 393 737 6 437 40 71 874 597
Cuts at: 4394 5181 5449 1239
Size: 787 268 1524
Fragments arranged by size:
1524 874 787 737 597 437 393 268
71 40 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2387 2387
Size: 5734
EcoO109I rG'GnC_Cy
Cuts at: 2020 2027 2307 2722 2793 2020
Size: 7 280 415 71 4961
Fragments arranged by size:
4961 415 280 71 7
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 4375 4407 4449
Size: 32 135 32 740 32 2031 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 2031 740 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 2758 3059 2758
Size: 301 5433
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2443 4848 5032 1305
Size: 1138 2405 184 2007
Fragments arranged by size:
2405 2007 1138 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 10
Size: 54 1425 343 219 3693
Fragments arranged by size:
3693 1425 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 3692 4051 4210 518
Size: 648 1045 1481 359 159 2042
Fragments arranged by size:
2042 1481 1045 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2252 3150 3827 4975 5220 1606
Size: 432 214 898 677 1148 245 2120
Fragments arranged by size:
2120 1148 898 677 432 245 214
NcoI C'CATG_G
Cuts at: 513 1065 2004 3017 513
Size: 552 939 1013 3230
Fragments arranged by size:
3230 1013 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 3092 4637 1132
Size: 97 1863 1545 2229
Fragments arranged by size:
2229 1863 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2398 2956 1348
Size: 1050 558 4126
Fragments arranged by size:
4126 1050 558
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PfoI T'CCnGG_A
Cuts at: 2385 2840 2385
Size: 455 5279
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2020
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PspOMI G'GGCC_C
Cuts at: 2307 2722 2793 2307
Size: 415 71 5248
Fragments arranged by size:
5248 415 71
PspXI vC'TCGA_Gb
Cuts at: 2576 2576
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2476 2571 3086 830
Size: 1646 95 515 3478
Fragments arranged by size:
3478 1646 515 95
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2038 2252 2038
Size: 214 5520
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2472 2567 3082 4217 4898 5089 826
Size: 219 1427 95 515 1135 681 191 1471
Fragments arranged by size:
1471 1427 1135 681 515 219 191 95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2576 3838 4739 5001 820
Size: 197 926 633 1262 901 262 1553
Fragments arranged by size:
1553 1262 926 901 633 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 2806 1517
Size: 1289 4445
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 3017 3173 513
Size: 552 939 336 677 156 3074
Fragments arranged by size:
3074 939 677 552 336 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 3761 3778 3931 4116 4561 325
Size: 1255 650 1531 17 153 185 445 1498
Fragments arranged by size:
1531 1498 1255 650 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 2814 3980
Size: 274 80 33 51 1443 639 198 1166
Cuts at: 3980 5692 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1166 639 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2654 2686 5694 5726 2654
Size: 32 3008 32 2662
Fragments arranged by size:
3008 2662 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2213 2714 2213
Size: 501 5233
XhoI C'TCGA_G
Cuts at: 1943 2576 1943
Size: 633 5101
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI