Restriction map for
pFN21AE4330
(Circular) (Six-base) MAPSORT of: pFN21AE4330.seq Check: 7987 from: 1 to: 5719
pFN21AE4330 5719 bp
With 182 enzymes: SgfI *
March 28, 2012 14:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2513 3529 278
Size: 53 83 186 936 977 1016 2468
Fragments arranged by size:
2468 1016 977 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2141 3062 1937
Size: 204 921 4594
Fragments arranged by size:
4594 921 204
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 2380 2536 2675 3782 5166 5434 1632
Size: 447 301 156 139 1107 1384 268 1917
Fragments arranged by size:
1917 1384 1107 447 301 268 156 139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 4618 5595 1203
Size: 3415 977 1327
Fragments arranged by size:
3415 1327 977
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 2514 4448 2514
Size: 1934 3785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2857 1184
Size: 1673 4046
AlwNI CAG_nnn'CTG
Cuts at: 2403 2498 2536 3462 5034 2403
Size: 95 38 926 1572 3088
Fragments arranged by size:
3088 1572 926 95 38
ApaLI G'TGCA_C
Cuts at: 2741 3776 4932 2741
Size: 1035 1156 3528
Fragments arranged by size:
3528 1156 1035
ApoI r'AATT_y
Cuts at: 1493 2180 2933 3028 3254 3406 5515 1493
Size: 687 753 95 226 152 2109 1697
Fragments arranged by size:
2109 1697 753 687 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 2736 3044 1870
Size: 73 471 322 308 4545
Fragments arranged by size:
4545 471 322 308 73
BamHI G'GATC_C
Cuts at: 2709 3049 3416 2709
Size: 340 367 5012
Fragments arranged by size:
5012 367 340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2885 3040 4495 618
Size: 325 217 627 1098 155 1455 1842
Fragments arranged by size:
1842 1455 1098 627 325 217 155
BanII G_rGCy'C
Cuts at: 1391 2394 2651 2989 3038 1391
Size: 1003 257 338 49 4072
Fragments arranged by size:
4072 1003 338 257 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2513 928
Size: 971 614 4134
Fragments arranged by size:
4134 971 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 2355 1548
Size: 807 4912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2055 2585 4330
Size: 107 122 71 631 605 383 530 1745
Cuts at: 4330 136
Size: 1525
Fragments arranged by size:
1745 1525 631 605 530 383 122 107
71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2745 2888 3780 4936 1790
Size: 955 143 892 1156 2573
Fragments arranged by size:
2573 1156 955 892 143
BmgBI CAC'GTC
Cuts at: 1318 2878 1318
Size: 1560 4159
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3450 4408 442
Size: 416 53 83 399 24 2033 958 1753
Fragments arranged by size:
2033 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2475 4379 1239
Size: 1236 1904 2579
Fragments arranged by size:
2579 1904 1236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 493
Size: 711 2306 2702
Fragments arranged by size:
2702 2306 711
BsaBI GATnn'nnATC
Cuts at: 5713 5713
Size: 5719
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2510 3526 3908 4543
Size: 53 83 186 936 977 1016 382 635
Cuts at: 4543 275
Size: 1451
Fragments arranged by size:
1451 1016 977 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2826 4152 4550 4824 4971 5382 2826
Size: 1326 398 274 147 411 3163
Fragments arranged by size:
3163 1326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2633 2663 1178
Size: 30 165 30 1230 30 4234
Fragments arranged by size:
4234 1230 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5719
BseYI C'CCAG_C
Cuts at: 2558 4922 5418 2558
Size: 2364 496 2859
Fragments arranged by size:
2859 2364 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 2510 2522 1163
Size: 573 653 121 12 4360
Fragments arranged by size:
4360 653 573 121 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2059 2070 3169 3930 4958 1540
Size: 275 186 58 11 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186 58 11
BsiHKAI G_wGCw'C
Cuts at: 2394 2598 2631 2745 2989 3780 3865 4936 2394
Size: 204 33 114 244 791 85 1071 3177
Fragments arranged by size:
3177 1071 791 244 204 114 85 33
BsmI GAATG_Cn'
Cuts at: 3230 3323 3230
Size: 93 5626
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2598 2631 2651 2745 2888 2989
Size: 399 604 204 33 20 94 143 101
Cuts at: 2989 3038 3780 3865 4936 1391
Size: 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 204 143 101
94 85 49 33 20
BspEI T'CCGG_A
Cuts at: 2826 5382 2826
Size: 2556 3163
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2544 3074 844
Size: 1700 530 3489
Fragments arranged by size:
3489 1700 530
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4214 4388 65
Size: 1183 2966 174 1396
Fragments arranged by size:
2966 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4363 4550 1700
Size: 110 2553 187 2869
Fragments arranged by size:
2869 2553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 3779 4791 3779
Size: 1012 4707
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 2324 4523 2324
Size: 2199 3520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 2801
Size: 5719
BstYI r'GATC_y
Cuts at: 1972 2709 2798 3049 3416 3801 3818 5270 5356
Size: 737 89 251 367 385 17 1452 86
Cuts at: 5356 5368 5714 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 737 385 367 346 251 89
86 17 12
BstZ17I GTA'TAC
Cuts at: 2142 2142
Size: 5719
BtgI C'CryG_G
Cuts at: 513 1065 2004 2704 2794 513
Size: 552 939 700 90 3438
Fragments arranged by size:
3438 939 700 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2603 487
Size: 683 56 1377 3603
Fragments arranged by size:
3603 1377 683 56
BtsI GCAGTG_nn'
Cuts at: 3235 4029 4049 3235
Size: 794 20 4905
Fragments arranged by size:
4905 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DraIII CAC_nnn'GTG
Cuts at: 2749 2749
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4726 809
Size: 507 3410 1802
Fragments arranged by size:
3410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2056 2067 2460
Size: 54 1425 50 275 18 226 11 393
Cuts at: 2460 3166 4055 5684 8
Size: 706 889 1629 43
Fragments arranged by size:
1629 1425 889 706 393 275 226 54
50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2056 2067 3166 1537
Size: 275 244 11 1099 4090
Fragments arranged by size:
4090 1099 275 244 11
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 3649 1353
Size: 1046 1250 3423
Fragments arranged by size:
3423 1250 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 2987 2392
Size: 595 5124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2380 2475 2536 2675 3782 4379
Size: 393 447 301 95 61 139 1107 597
Cuts at: 4379 5166 5434 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1107 787 597 447 393 301 268
139 95 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 5719
EcoO109I rG'GnC_Cy
Cuts at: 2533 2533
Size: 5719
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 5719
FspI TGC'GCA
Cuts at: 4225 4225
Size: 5719
HaeII r_GCGC'y
Cuts at: 1678 4866 1678
Size: 3188 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2500 2532 4360 4392 4434
Size: 32 135 32 928 32 1828 32 42
Cuts at: 4434 4466 1373
Size: 32 2626
Fragments arranged by size:
2626 1828 928 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2692 3063 3309 669
Size: 988 281 754 371 246 3079
Fragments arranged by size:
3079 988 754 371 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 2692 3309 2692
Size: 617 5102
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4833 5017 1305
Size: 3528 184 2007
Fragments arranged by size:
3528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 10
Size: 54 1425 343 630 3267
Fragments arranged by size:
3267 1425 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 3677 4036 4195 518
Size: 648 1710 801 359 159 2042
Fragments arranged by size:
2042 1710 801 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3135 3812 4960 5205 1606
Size: 1529 677 1148 245 2120
Fragments arranged by size:
2120 1529 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4228
Fragments arranged by size:
4228 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 3077 4622 1132
Size: 97 1848 1545 2229
Fragments arranged by size:
2229 1848 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2818 1348
Size: 1470 4249
PciI A'CATG_T
Cuts at: 4618 4618
Size: 5719
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2498 1108
Size: 1390 4329
PfoI T'CCnGG_A
Cuts at: 2367 2376 2712 2367
Size: 9 336 5374
Fragments arranged by size:
5374 336 9
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 2533
Size: 5719
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PstI C_TGCA'G
Cuts at: 830 2037 2273 3071 830
Size: 1207 236 798 3478
Fragments arranged by size:
3478 1207 798 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
SacI G_AGCT'C
Cuts at: 2394 2989 2394
Size: 595 5124
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2399 2399
Size: 5719
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SexAI A'CCwGG_T
Cuts at: 2745 2745
Size: 5719
SfcI C'TryA_G
Cuts at: 826 1045 2033 2269 3067 4202 4883 5074 826
Size: 219 988 236 798 1135 681 191 1471
Fragments arranged by size:
1471 1135 988 798 681 236 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2055 2055
Size: 5719
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5719
SmaI CCC'GGG
Cuts at: 1872 2738 3046 1872
Size: 866 308 4545
Fragments arranged by size:
4545 866 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3823 4724 4986 820
Size: 197 926 1880 901 262 1553
Fragments arranged by size:
1880 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 2455 3643 5
Size: 47 2403 1188 2081
Fragments arranged by size:
2403 2081 1188 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2004 3158 513
Size: 552 939 1154 3074
Fragments arranged by size:
3074 1154 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3746 3763 3916 4101 4546 325
Size: 1255 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3965 5677 96
Size: 274 80 33 51 1443 1988 1712 138
Fragments arranged by size:
1988 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2633 2665 5679 5711 2633
Size: 32 3014 32 2641
Fragments arranged by size:
3014 2641 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5719
XmaI C'CCGG_G
Cuts at: 1870 2736 3044 1870
Size: 866 308 4545
Fragments arranged by size:
4545 866 308
XmnI GAAnn'nnTTC
Cuts at: 3848 3848
Size: 5719
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2511 3527 276
Size: 53 83 186 936 977 1016 2468
Fragments arranged by size:
2468 1016 977 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SfoI SrfI SwaI XcmI