Restriction map for
pFN21AE4329
(Circular) (Six-base) MAPSORT of: pFN21AE4329.seq Check: 5469 from: 1 to: 5707
pFN21AE4329 5707 bp
With 182 enzymes: SgfI *
March 28, 2012 13:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2618 2618
Size: 5707
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 3770 5154 5422 1632
Size: 575 1563 1384 268 1917
Fragments arranged by size:
1917 1563 1384 575 268
AflII C'TTAA_G
Cuts at: 820 1017 2015 820
Size: 197 998 4512
Fragments arranged by size:
4512 998 197
AflIII A'CryG_T
Cuts at: 1203 4606 5583 1203
Size: 3403 977 1327
Fragments arranged by size:
3403 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 2081 1166
Size: 915 4792
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2559 3450 5022 2559
Size: 891 1572 3244
Fragments arranged by size:
3244 1572 891
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2360 3016 3242 3394 5503 1493
Size: 867 656 226 152 2109 1697
Fragments arranged by size:
2109 1697 867 656 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2898 3032 1870
Size: 73 955 134 4545
Fragments arranged by size:
4545 955 134 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3026 1391
Size: 1635 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2347 2860 928
Size: 971 448 513 3775
Fragments arranged by size:
3775 971 513 448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2897 3599 4809 2897
Size: 702 1210 3795
Fragments arranged by size:
3795 1210 702
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 2949 3124 2949
Size: 175 5532
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2417 2764 3438
Size: 416 53 83 399 24 1000 347 674
Cuts at: 3438 4396 442
Size: 958 1753
Fragments arranged by size:
1753 1000 958 674 416 399 347 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 4367 1239
Size: 1385 1743 2579
Fragments arranged by size:
2579 1743 1385
Bpu10I CC'TnA_GC
Cuts at: 2873 2873
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2578 2939 3832 4697 4995 2578
Size: 361 893 865 298 3290
Fragments arranged by size:
3290 893 865 361 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2439 3498 493
Size: 711 1235 1059 2702
Fragments arranged by size:
2702 1235 1059 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5482
Fragments arranged by size:
5482 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 2552 4910 5406 2552
Size: 2358 496 2853
Fragments arranged by size:
2853 2358 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 2985 1163
Size: 573 621 628 3885
Fragments arranged by size:
3885 628 621 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3768 3853 4924 3768
Size: 85 1071 4551
Fragments arranged by size:
4551 1071 85
BsmI GAATG_Cn'
Cuts at: 2121 2544 3218 3311 2121
Size: 423 674 93 4517
Fragments arranged by size:
4517 674 423 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3026 3768 3853 4924 1391
Size: 399 1236 742 85 1071 2174
Fragments arranged by size:
2174 1236 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2618 3062 844
Size: 1774 444 3489
Fragments arranged by size:
3489 1774 444
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2028 4202 4376 65
Size: 1183 780 2174 174 1396
Fragments arranged by size:
2174 1396 1183 780 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2039 794
Size: 1245 4462
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 2425
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2197 3037 3404 3789 3806 5258 5344 5356
Size: 225 840 367 385 17 1452 86 12
Cuts at: 5356 5702 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 840 385 367 346 225 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2563 513
Size: 552 939 559 3657
Fragments arranged by size:
3657 939 559 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4017 4037 3223
Size: 794 20 4893
Fragments arranged by size:
4893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2479 2479
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672
Size: 54 1425 50 275 18 1324 889 1629
Cuts at: 5672 8
Size: 43
Fragments arranged by size:
1629 1425 1324 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2043 3637 1353
Size: 690 1594 3423
Fragments arranged by size:
3423 1594 690
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2624 3770 4367 5154 5422 1239
Size: 393 575 417 1146 597 787 268 1524
Fragments arranged by size:
1524 1146 787 597 575 417 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2992 2992
Size: 5707
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2195 2195
Size: 5707
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3051 3297 669
Size: 988 281 891 222 246 3079
Fragments arranged by size:
3079 988 891 281 246 222
HindIII A'AGCT_T
Cuts at: 748 2177 748
Size: 1429 4278
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2071 2442 4821 5005 1305
Size: 766 371 2379 184 2007
Fragments arranged by size:
2379 2007 766 371 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2408 3665 4024 4183 518
Size: 648 915 327 1257 359 159 2042
Fragments arranged by size:
2042 1257 915 648 359 327 159
MspA1I CmG'CkG
Cuts at: 1606 2559 3123 3800 4948 5193 1606
Size: 953 564 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 677 564 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2563 513
Size: 552 939 559 3657
Fragments arranged by size:
3657 939 559 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PfoI T'CCnGG_A
Cuts at: 2640 2810 2640
Size: 170 5537
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2992 2992
Size: 5707
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 2205 2296 3059 830
Size: 1375 91 763 3478
Fragments arranged by size:
3478 1375 763 91
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2559 2559
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SexAI A'CCwGG_T
Cuts at: 3003 3003
Size: 5707
SfcI C'TryA_G
Cuts at: 826 1045 2079 2201 2292 3055 4190 4871 5062
Size: 219 1034 122 91 763 1135 681 191
Cuts at: 5062 826
Size: 1471
Fragments arranged by size:
1471 1135 1034 763 681 219 191 122
91
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 2900 3034 1872
Size: 1028 134 4545
Fragments arranged by size:
4545 1028 134
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 2593 2954 3811 4712 4974
Size: 197 926 72 578 361 857 901 262
Cuts at: 4974 820
Size: 1553
Fragments arranged by size:
1553 926 901 857 578 361 262 197
72
SnaBI TAC'GTA
Cuts at: 493 2439 3498 493
Size: 1946 1059 2702
Fragments arranged by size:
2702 1946 1059
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 2563 3146 513
Size: 552 939 559 583 3074
Fragments arranged by size:
3074 939 583 559 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 3734 3751 3904 4089 4534 325
Size: 1255 495 1659 17 153 185 445 1498
Fragments arranged by size:
1659 1498 1255 495 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 3953 5665
Size: 274 80 33 51 1443 408 1568 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1568 1443 408 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 2757 2789 5667 5699 2443
Size: 32 282 32 2878 32 2451
Fragments arranged by size:
2878 2451 282 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 2898 3032 1870
Size: 1028 134 4545
Fragments arranged by size:
4545 1028 134
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI