Restriction map for
pFN21AE4328
(Circular) (Six-base) MAPSORT of: pFN21AE4328.seq Check: 732 from: 1 to: 5707
pFN21AE4328 5707 bp
With 182 enzymes: SgfI *
March 28, 2012 13:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 2252 2376 3028 2252
Size: 124 652 4931
Fragments arranged by size:
4931 652 124
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3770 5154 5422 1632
Size: 2138 1384 268 1917
Fragments arranged by size:
2138 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2779 820
Size: 197 1762 3748
Fragments arranged by size:
3748 1762 197
AflIII A'CryG_T
Cuts at: 1203 2246 4606 5583 1203
Size: 1043 2360 977 1327
Fragments arranged by size:
2360 1327 1043 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 2039
Size: 32 5675
AlwNI CAG_nnn'CTG
Cuts at: 2651 3450 5022 2651
Size: 799 1572 3336
Fragments arranged by size:
3336 1572 799
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2350 2389 2461 2538 2628 2750 2840 3016
Size: 857 39 72 77 90 122 90 176
Cuts at: 3016 3242 3394 5503 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 857 226 176 152 122 90
90 77 72 39
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 3032 1870
Size: 73 1089 4545
Fragments arranged by size:
4545 1089 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2252 2376 3028 4483 618
Size: 325 217 627 465 124 652 1455 1842
Fragments arranged by size:
1842 1455 652 627 465 325 217 124
BanII G_rGCy'C
Cuts at: 1391 3026 1391
Size: 1635 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2249 2818 928
Size: 971 350 569 3817
Fragments arranged by size:
3817 971 569 350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 2805 1548
Size: 1257 4450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2114 5702 2114
Size: 3588 2119
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3438 4396 442
Size: 416 53 83 399 24 2021 958 1753
Fragments arranged by size:
2021 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2724 4367 1239
Size: 827 658 1643 2579
Fragments arranged by size:
2579 1643 827 658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4370 882
Size: 617 2871 2219
Fragments arranged by size:
2871 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2385 4140 4538 4812 4959 5370 2385
Size: 1755 398 274 147 411 2722
Fragments arranged by size:
2722 1755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 1178
Size: 30 165 30 636 30 4816
Fragments arranged by size:
4816 636 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5707
BseYI C'CCAG_C
Cuts at: 2560 4910 5406 2560
Size: 2350 496 2861
Fragments arranged by size:
2861 2350 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1998 1163
Size: 573 262 4872
Fragments arranged by size:
4872 573 262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3768 3853 4924 3768
Size: 85 1071 4551
Fragments arranged by size:
4551 1071 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
BsmBI CGTCTCn'nnnn_
Cuts at: 2221 2221
Size: 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3026 3768 3853 4924 1391
Size: 399 1236 742 85 1071 2174
Fragments arranged by size:
2174 1236 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 2355 3596 5326 2355
Size: 1241 1730 2736
Fragments arranged by size:
2736 1730 1241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2153 4202 4376 65
Size: 1183 905 2049 174 1396
Fragments arranged by size:
2049 1396 1183 905 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 2616 3767 4779 2616
Size: 1151 1012 3544
Fragments arranged by size:
3544 1151 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 2025 4511 2025
Size: 2486 3221
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstXI CCAn_nnnn'nTGG
Cuts at: 2090 2090
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2087 2095 2114 3037 3404 3789 3806 5258
Size: 115 8 19 923 367 385 17 1452
Cuts at: 5258 5344 5356 5702 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 923 385 367 346 115 86
19 17 12 8
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 4017 4037 3223
Size: 794 20 4893
Fragments arranged by size:
4893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2662 2697 2839 2874 529
Size: 35 2098 35 142 35 3362
Fragments arranged by size:
3362 2098 142 35 35 35
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3154 4043 5672
Size: 54 1425 50 275 18 1324 889 1629
Cuts at: 5672 8
Size: 43
Fragments arranged by size:
1629 1425 1324 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3637 1353
Size: 2284 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2724 3770 4367 5154 5422 1239
Size: 393 434 658 1046 597 787 268 1524
Fragments arranged by size:
1524 1046 787 658 597 434 393 268
EcoRI G'AATT_C
Cuts at: 2461 2628 3016 3394 2461
Size: 167 388 378 4774
Fragments arranged by size:
4774 388 378 167
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373
Size: 32 135 32 2776 32 42 32 2626
Fragments arranged by size:
2776 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2187 2291 2329 748
Size: 1439 104 38 4126
Fragments arranged by size:
4126 1439 104 38
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 2256 2380 3032 2256
Size: 124 652 4931
Fragments arranged by size:
4931 652 124
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4821 5005 1305
Size: 3516 184 2007
Fragments arranged by size:
3516 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 3665 4024 4183 518
Size: 648 996 1503 359 159 2042
Fragments arranged by size:
2042 1503 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3123 3800 4948 5193 1606
Size: 1517 677 1148 245 2120
Fragments arranged by size:
2120 1517 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2215 2250 3065 4610 1132
Size: 97 986 35 815 1545 2229
Fragments arranged by size:
2229 1545 986 815 97 35
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 2246 4606 2246
Size: 2360 3347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2071 3776 3808 5289 5321 2039
Size: 32 1705 32 1481 32 2425
Fragments arranged by size:
2425 1705 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SexAI A'CCwGG_T
Cuts at: 2644 2644
Size: 5707
SfcI C'TryA_G
Cuts at: 826 1045 3055 4190 4871 5062 826
Size: 219 2010 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2779 3811 4712 4974 820
Size: 197 926 836 1032 901 262 1553
Fragments arranged by size:
1553 1032 926 901 836 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 2083 2371 2532 3146 513
Size: 552 939 79 288 161 614 3074
Fragments arranged by size:
3074 939 614 552 288 161 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 3734 3751 3904 4089 4534 325
Size: 1255 468 1686 17 153 185 445 1498
Fragments arranged by size:
1686 1498 1255 468 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2194 3953 5665
Size: 274 80 33 51 1443 217 1759 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1759 1712 1443 274 217 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 5667 5699 2829
Size: 32 2806 32 2837
Fragments arranged by size:
2837 2806 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2118 2118
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI