Restriction map for
pFN21AE4326
(Circular) (Six-base) MAPSORT of: pFN21AE4326.seq Check: 3919 from: 1 to: 5689
pFN21AE4326 5689 bp
With 182 enzymes: SgfI *
March 28, 2012 14:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2313 3032 1937
Size: 376 719 4594
Fragments arranged by size:
4594 719 376
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3752 5136 5404 1632
Size: 2120 1384 268 1917
Fragments arranged by size:
2120 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 2517 4588 5565 1203
Size: 1314 2071 977 1327
Fragments arranged by size:
2071 1327 1314 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 2114
Size: 32 5657
AlwNI CAG_nnn'CTG
Cuts at: 2338 2383 2704 2716 3432 5004 2338
Size: 45 321 12 716 1572 3023
Fragments arranged by size:
3023 1572 716 321 45 12
ApaI G_GGCC'C
Cuts at: 2178 2759 2178
Size: 581 5108
ApaLI G'TGCA_C
Cuts at: 2969 3746 4902 2969
Size: 777 1156 3756
Fragments arranged by size:
3756 1156 777
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2153 2356 2924 3014 1870
Size: 73 210 203 568 90 4545
Fragments arranged by size:
4545 568 210 203 90 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2432 3010 4465 618
Size: 325 217 627 435 210 578 1455 1842
Fragments arranged by size:
1842 1455 627 578 435 325 217 210
BanII G_rGCy'C
Cuts at: 1391 2178 2247 2363 2658 2759 2905 3008 1391
Size: 787 69 116 295 101 146 103 4072
Fragments arranged by size:
4072 787 295 146 116 103 101 69
BbeI G_GCGC'C
Cuts at: 2226 2436 2226
Size: 210 5479
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2942 2976 3880 3914 1903
Size: 34 50 34 921 34 904 34 3678
Fragments arranged by size:
3678 921 904 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2204 2213 4300
Size: 107 122 71 631 605 532 9 2087
Cuts at: 4300 136
Size: 1525
Fragments arranged by size:
2087 1525 631 605 532 122 107 71
9
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2402 2759 2973 3750 4906 1790
Size: 388 224 357 214 777 1156 2573
Fragments arranged by size:
2573 1156 777 388 357 224 214
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2096 3420 4378
Size: 416 53 83 399 24 679 1324 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1324 958 679 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2669 2738 2783 4349 1239
Size: 1073 357 69 45 1566 2579
Fragments arranged by size:
2579 1566 1073 357 69 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 3814 4679 4977 2540
Size: 1274 865 298 3252
Fragments arranged by size:
3252 1274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2500 3480 493
Size: 711 1296 980 2702
Fragments arranged by size:
2702 1296 980 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 2310 2421 2433
Size: 53 83 186 936 690 87 111 12
Cuts at: 2433 3496 3878 4513 275
Size: 1063 382 635 1451
Fragments arranged by size:
1451 1063 936 690 635 382 186 111
87 83 53 12
BsaWI w'CCGG_w
Cuts at: 2743 4122 4520 4794 4941 5352 2743
Size: 1379 398 274 147 411 3080
Fragments arranged by size:
3080 1379 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 2345 2375 2472
Size: 30 165 30 711 30 201 30 97
Cuts at: 2472 2502 2759 2789 2849 2879 1178
Size: 30 257 30 60 30 3988
Fragments arranged by size:
3988 711 257 201 165 97 60 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 1186
Size: 1480 4209
BseYI C'CCAG_C
Cuts at: 2976 4892 5388 2976
Size: 1916 496 3277
Fragments arranged by size:
3277 1916 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2131 2765 1163
Size: 573 395 634 4087
Fragments arranged by size:
4087 634 573 395
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2769 2973 3750 3835 4906 2247
Size: 522 204 777 85 1071 3030
Fragments arranged by size:
3030 1071 777 522 204 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2247 2363 2402 2658 2759 2769
Size: 399 388 69 116 39 256 101 10
Cuts at: 2769 2905 2973 3008 3750 3835 4906 1391
Size: 136 68 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 388 256 136 116
101 85 69 68 39 35 10
BspEI T'CCGG_A
Cuts at: 2743 5352 2743
Size: 2609 3080
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2437 2977 3044 844
Size: 1593 540 67 3489
Fragments arranged by size:
3489 1593 540 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2722 2752 3576 1394
Size: 429 899 30 824 3507
Fragments arranged by size:
3507 899 824 429 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2182 2610 4333 4520 1700
Size: 110 372 428 1723 187 2869
Fragments arranged by size:
2869 1723 428 372 187 110
BsrGI T'GTAC_A
Cuts at: 96 2811 96
Size: 2715 2974
BssHII G'CGCG_C
Cuts at: 1925 2114 2116 1925
Size: 189 2 5498
Fragments arranged by size:
5498 189 2
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2235 794
Size: 1441 4248
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2724 3019 3386 3771 3788 5240 5326 5338
Size: 752 295 367 385 17 1452 86 12
Cuts at: 5338 5684 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 752 385 367 346 295 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2199 513
Size: 552 1134 4003
Fragments arranged by size:
4003 1134 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2478 487
Size: 683 56 1252 3698
Fragments arranged by size:
3698 1252 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 2542 3205 3999 4019 2217
Size: 325 663 794 20 3887
Fragments arranged by size:
3887 794 663 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 2205 2214
Size: 54 1425 50 275 18 333 42 9
Cuts at: 2214 2329 2542 2577 3136 4025 5654 8
Size: 115 213 35 559 889 1629 43
Fragments arranged by size:
1629 1425 889 559 333 275 213 115
54 50 43 42 35 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2942 3619 1353
Size: 1589 677 3423
Fragments arranged by size:
3423 1589 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2669 2738 2783 3752 4349 5136
Size: 393 680 357 69 45 969 597 787
Cuts at: 5136 5404 1239
Size: 268 1524
Fragments arranged by size:
1524 969 787 680 597 393 357 268
69 45
EcoO109I rG'GnC_Cy
Cuts at: 2175 2713 2755 2756 2920 2175
Size: 538 42 1 164 4944
Fragments arranged by size:
4944 538 164 42 1
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2161 2226 2384 2416 2436 4836 1678
Size: 483 65 158 32 20 2400 2531
Fragments arranged by size:
2531 2400 483 158 65 32 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 4330 4362 4404
Size: 32 135 32 884 32 1842 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1842 884 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2222 2432 2222
Size: 210 5479
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2516 3647 4006 4165 518
Size: 648 1350 1131 359 159 2042
Fragments arranged by size:
2042 1350 1131 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2053 2062 2071 2136 2201 2980 3105
Size: 432 15 9 9 65 65 779 125
Cuts at: 3105 3782 4930 5175 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 779 677 432 245 125 65
65 15 9 9
NarI GG'CG_CC
Cuts at: 2223 2433 2223
Size: 210 5479
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2233 2521 3047 4592 1132
Size: 97 1004 288 526 1545 2229
Fragments arranged by size:
2229 1545 1004 526 288 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 2517 4588 2517
Size: 2071 3618
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 1108
Size: 1608 4081
PfoI T'CCnGG_A
Cuts at: 2250 2586 2715 2250
Size: 336 129 5224
Fragments arranged by size:
5224 336 129
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PmlI CAC'GTG
Cuts at: 2500 2500
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2114 2146 3758 3790 5271 5303 2114
Size: 32 1612 32 1481 32 2500
Fragments arranged by size:
2500 1612 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2713 2920 2713
Size: 207 5482
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2174 2755 2174
Size: 581 5108
PstI C_TGCA'G
Cuts at: 830 2379 3041 830
Size: 1549 662 3478
Fragments arranged by size:
3478 1549 662
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 5689
SacII CC_GC'GG
Cuts at: 2202 2202
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SanDI GG'GwC_CC
Cuts at: 2920 2920
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 2379 3041 2379
Size: 662 5027
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SexAI A'CCwGG_T
Cuts at: 2709 2709
Size: 5689
SfcI C'TryA_G
Cuts at: 826 1045 2375 3037 4172 4853 5044 826
Size: 219 1330 662 1135 681 191 1471
Fragments arranged by size:
1471 1330 1135 681 662 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2213 2213
Size: 5689
SfoI GGC'GCC
Cuts at: 2224 2434 2224
Size: 210 5479
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2358 3016 1872
Size: 486 658 4545
Fragments arranged by size:
4545 658 486
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2555 3793 4694 4956 820
Size: 197 926 612 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 612 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 2233 3047 1132
Size: 1101 814 3774
Fragments arranged by size:
3774 1101 814
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2484 1517
Size: 967 4722
StyI C'CwwG_G
Cuts at: 513 1065 2641 3128 513
Size: 552 1576 487 3074
Fragments arranged by size:
3074 1576 552 487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2990 3716 3733 3886 4071 4516 325
Size: 1255 1410 726 17 153 185 445 1498
Fragments arranged by size:
1498 1410 1255 726 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2811 3935 5647
Size: 274 80 33 51 1443 834 1124 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1443 1124 834 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2345 2377 2472 2504 2759 2791 2849 2881 2912
Size: 32 95 32 255 32 58 32 31
Cuts at: 2912 2944 5649 5681 2345
Size: 32 2705 32 2353
Fragments arranged by size:
2705 2353 255 95 58 32 32 32
32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 2711 1316
Size: 1395 4294
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2518 2518
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2356 3014 1870
Size: 486 658 4545
Fragments arranged by size:
4545 658 486
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI PvuII RsrII SapI SrfI SwaI