Restriction map for
pFN21AE4325
(Circular) (Six-base) MAPSORT of: pFN21AE4325.seq Check: 36 from: 1 to: 5689
pFN21AE4325 5689 bp
With 182 enzymes: SgfI *
March 28, 2012 14:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 2945 3816 4189 1563
Size: 1382 871 373 3063
Fragments arranged by size:
3063 1382 871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 2897 3752 5136 5404 1632
Size: 764 501 855 1384 268 1917
Fragments arranged by size:
1917 1384 855 764 501 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 2986 4520 2986
Size: 1534 4155
AhdI GACnn_n'nnGTC
Cuts at: 2972 4418 2972
Size: 1446 4243
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2161 1184
Size: 977 4712
AlwNI CAG_nnn'CTG
Cuts at: 2245 2374 3432 5004 2245
Size: 129 1058 1572 2930
Fragments arranged by size:
2930 1572 1058 129
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2683 2998 3224 3376 5485 1493
Size: 1190 315 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 315 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2141 4243 160
Size: 872 1109 2102 1606
Fragments arranged by size:
2102 1606 1109 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2073 2110 3014 1870
Size: 73 130 37 904 4545
Fragments arranged by size:
4545 904 130 73 37
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 3010 4465 618
Size: 325 217 627 423 800 1455 1842
Fragments arranged by size:
1842 1455 800 627 423 325 217
BanII G_rGCy'C
Cuts at: 1391 2862 3008 1391
Size: 1471 146 4072
Fragments arranged by size:
4072 1471 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2418 5684 2418
Size: 3266 2423
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2524 3750 4906 1790
Size: 423 311 1226 1156 2573
Fragments arranged by size:
2573 1226 1156 423 311
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 4349 1239
Size: 1271 1839 2579
Fragments arranged by size:
2579 1839 1271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2577 3814 4679 4977 2577
Size: 1237 865 298 3289
Fragments arranged by size:
3289 1237 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 2417 5683 2417
Size: 3266 2423
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2093 2986 4122 4520 4794 4941 5352 2093
Size: 893 1136 398 274 147 411 2430
Fragments arranged by size:
2430 1136 893 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 1178
Size: 30 165 30 1311 30 4123
Fragments arranged by size:
4123 1311 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2073 1186
Size: 887 4802
BseYI C'CCAG_C
Cuts at: 2870 4892 5388 2870
Size: 2022 496 3171
Fragments arranged by size:
3171 2022 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 3835 4906 3750
Size: 85 1071 4533
Fragments arranged by size:
4533 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2524 2862 3008 3750 3835 4906
Size: 399 423 311 338 146 742 85 1071
Cuts at: 4906 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 423 399 338 311 146
85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2986 4333 4520 1700
Size: 110 1176 1347 187 2869
Fragments arranged by size:
2869 1347 1176 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 2701 1184
Size: 1517 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2649 2011
Size: 638 5051
BstYI r'GATC_y
Cuts at: 1972 2328 2412 2418 2514 3019 3386 3771 3788
Size: 356 84 6 96 505 367 385 17
Cuts at: 3788 5240 5326 5338 5684 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 505 385 367 356 346 96
86 84 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2505 2993 2505
Size: 488 5201
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654
Size: 54 1425 50 275 18 1306 889 1629
Cuts at: 5654 8
Size: 43
Fragments arranged by size:
1629 1425 1306 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3619 1353
Size: 2266 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2396 2510 2897 3752 4349 5136 5404
Size: 393 764 114 387 855 597 787 268
Cuts at: 5404 1239
Size: 1524
Fragments arranged by size:
1524 855 787 764 597 393 387 268
114
EcoNI CCTnn'n_nnAGG
Cuts at: 2180 2180
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2023 2023
Size: 5689
EcoRI G'AATT_C
Cuts at: 2683 2998 3376 2683
Size: 315 378 4996
Fragments arranged by size:
4996 378 315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 5689
FspI TGC'GCA
Cuts at: 2034 4195 2034
Size: 2161 3528
FspAI rTGC'GCAy
Cuts at: 2034 2034
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2958 2990 4330 4362 4404
Size: 32 135 32 1386 32 1340 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1386 1340 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3647 4006 4165 518
Size: 648 843 1638 359 159 2042
Fragments arranged by size:
2042 1638 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2318 2741 3105 3782 4930 5175 1606
Size: 712 423 364 677 1148 245 2120
Fragments arranged by size:
2120 1148 712 677 423 364 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PfoI T'CCnGG_A
Cuts at: 2678 2678
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2156 2234 1613
Size: 543 78 5068
Fragments arranged by size:
5068 543 78
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2609 2641 2609
Size: 32 5657
PstI C_TGCA'G
Cuts at: 830 2184 3041 830
Size: 1354 857 3478
Fragments arranged by size:
3478 1354 857
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2318 2318
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 2606 3937 5649 2606
Size: 1331 1712 2646
Fragments arranged by size:
2646 1712 1331
SexAI A'CCwGG_T
Cuts at: 2648 2648
Size: 5689
SfcI C'TryA_G
Cuts at: 826 1045 2180 2579 3037 4172 4853 5044 826
Size: 219 1135 399 458 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 681 458 399 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2112 3016 1872
Size: 240 904 4545
Fragments arranged by size:
4545 904 240
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2592 3793 4694 4956 820
Size: 197 926 649 1201 901 262 1553
Fragments arranged by size:
1553 1201 926 901 649 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 2918 1517
Size: 1401 4288
StyI C'CwwG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 3074
Fragments arranged by size:
3074 2063 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2137 2791 3716 3733 3886 4071 4516
Size: 1255 557 654 925 17 153 185 445
Cuts at: 4516 325
Size: 1498
Fragments arranged by size:
1498 1255 925 654 557 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2604 3935 5647
Size: 274 80 33 51 1443 627 1331 1712
Cuts at: 5647 96
Size: 138
Fragments arranged by size:
1712 1443 1331 627 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2112 2144 2839 2871 5649 5681 2112
Size: 32 695 32 2778 32 2120
Fragments arranged by size:
2778 2120 695 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2110 3014 1870
Size: 240 904 4545
Fragments arranged by size:
4545 904 240
XmnI GAAnn'nnTTC
Cuts at: 2606 3818 2606
Size: 1212 4477
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI