Restriction map for
pFN21AE4324
(Circular) (Six-base) MAPSORT of: pFN21AE4324.seq Check: 4280 from: 1 to: 5683
pFN21AE4324 5683 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2192 2192
Size: 5683
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2622 3026 1937
Size: 685 404 4594
Fragments arranged by size:
4594 685 404
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 2099 4582 5559 1203
Size: 896 2483 977 1327
Fragments arranged by size:
2483 1327 977 896
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2381 1184
Size: 1197 4486
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2217 2249 2217
Size: 32 5651
AlwNI CAG_nnn'CTG
Cuts at: 3426 4998 3426
Size: 1572 4111
ApaI G_GGCC'C
Cuts at: 2182 2271 2182
Size: 89 5594
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2164 2174 3008 1870
Size: 73 88 133 10 834 4545
Fragments arranged by size:
4545 834 133 88 73 10
BamHI G'GATC_C
Cuts at: 3013 3380 3013
Size: 367 5316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3004 4459 618
Size: 325 217 627 1217 1455 1842
Fragments arranged by size:
1842 1455 1217 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2182 2245 2271 2616 3002 1391
Size: 744 47 63 26 345 386 4072
Fragments arranged by size:
4072 744 386 345 63 47 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2837 2871 3874 3908 1903
Size: 34 900 34 1003 34 3678
Fragments arranged by size:
3678 1003 900 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 2130 4294
Size: 107 122 71 631 605 355 103 2164
Cuts at: 4294 136
Size: 1525
Fragments arranged by size:
2164 1525 631 605 355 122 107 103
71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 3100 3100
Size: 5683
Bme1580I G_kGCm'C
Cuts at: 1790 2182 2271 3744 4900 1790
Size: 392 89 1473 1156 2573
Fragments arranged by size:
2573 1473 1156 392 89
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5683
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2527 2720 3414
Size: 416 53 83 399 24 1110 193 694
Cuts at: 3414 4372 442
Size: 958 1753
Fragments arranged by size:
1753 1110 958 694 416 399 193 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2259 2941 4343 1239
Size: 908 112 682 1402 2579
Fragments arranged by size:
2579 1402 908 682 112
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 5683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2237 2271 2879 4346 882
Size: 617 543 195 34 608 1467 2219
Fragments arranged by size:
2219 1467 617 608 543 195 34
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2217 2247 2706 2736 2756
Size: 30 165 30 814 30 459 30 20
Cuts at: 2756 2786 1178
Size: 30 4075
Fragments arranged by size:
4075 814 459 165 30 30 30 30
30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2235 2298 1186
Size: 1049 63 4571
Fragments arranged by size:
4571 1049 63
BseYI C'CCAG_C
Cuts at: 2318 2551 2566 4886 5382 2318
Size: 233 15 2320 496 2619
Fragments arranged by size:
2619 2320 496 233 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 2616 3744 3829 4900 2439
Size: 177 1128 85 1071 3222
Fragments arranged by size:
3222 1128 1071 177 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
BsmBI CGTCTCn'nnnn_
Cuts at: 2307 2307
Size: 5683
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2182 2245 2271 2439 2616 3002
Size: 399 345 47 63 26 168 177 386
Cuts at: 3002 3744 3829 4900 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 386 345 177 168
85 63 47 26
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2085 2192 2373 2418 3038 844
Size: 1241 107 181 45 620 3489
Fragments arranged by size:
3489 1241 620 181 107 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2391 3570 1394
Size: 429 568 1179 3507
Fragments arranged by size:
3507 1179 568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2421 4327 4514 1700
Size: 110 611 1906 187 2869
Fragments arranged by size:
2869 1906 611 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5683
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 2807 4487 2807
Size: 1680 4003
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678
Size: 1041 367 385 17 1452 86 12 346
Cuts at: 5678 1972
Size: 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 513
Size: 552 939 21 4171
Fragments arranged by size:
4171 939 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2737 3199 3993 4013 2737
Size: 462 794 20 4407
Fragments arranged by size:
4407 794 462 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4690 809
Size: 507 3374 1802
Fragments arranged by size:
3374 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648
Size: 54 1425 50 275 18 1300 889 1629
Cuts at: 5648 8
Size: 43
Fragments arranged by size:
1629 1425 1300 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3613 1353
Size: 2260 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2614 2614
Size: 5683
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2259 2941 3746 4343 5130 5398
Size: 393 515 112 682 805 597 787 268
Cuts at: 5398 1239
Size: 1524
Fragments arranged by size:
1524 805 787 682 597 515 393 268
112
EcoO109I rG'GnC_Cy
Cuts at: 2178 2179 2267 2362 2888 2178
Size: 1 88 95 526 4973
Fragments arranged by size:
4973 526 95 88 1
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 5683
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 4830 1678
Size: 3152 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2706 2738 4324 4356 4398
Size: 32 135 32 1134 32 1586 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 1586 1134 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4797 4981 1305
Size: 3492 184 2007
Fragments arranged by size:
3492 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2482 2551 3099 3776 4924 5169 1606
Size: 876 69 548 677 1148 245 2120
Fragments arranged by size:
2120 1148 876 677 548 245 69
NaeI GCC'GGC
Cuts at: 2423 2423
Size: 5683
NcoI C'CATG_G
Cuts at: 513 1065 2004 2025 513
Size: 552 939 21 4171
Fragments arranged by size:
4171 939 552 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NgoMIV G'CCGG_C
Cuts at: 2421 2421
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 2103 3041 4586 1132
Size: 97 874 938 1545 2229
Fragments arranged by size:
2229 1545 938 874 97
PasI CC'CwG_GG
Cuts at: 1348 2183 2599 1348
Size: 835 416 4432
Fragments arranged by size:
4432 835 416
PciI A'CATG_T
Cuts at: 2099 4582 2099
Size: 2483 3200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5683
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2217 2249 3752 3784 5265 5297 2217
Size: 32 1503 32 1481 32 2603
Fragments arranged by size:
2603 1503 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2888 2362
Size: 526 5157
PshAI GACnn'nnGTC
Cuts at: 1613 2100 1613
Size: 487 5196
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PspOMI G'GGCC_C
Cuts at: 2178 2267 2178
Size: 89 5594
PstI C_TGCA'G
Cuts at: 830 3035 830
Size: 2205 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2551 2551
Size: 5683
SacI G_AGCT'C
Cuts at: 2616 2616
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SanDI GG'GwC_CC
Cuts at: 2888 2888
Size: 5683
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SexAI A'CCwGG_T
Cuts at: 2625 2625
Size: 5683
SfcI C'TryA_G
Cuts at: 826 1045 3031 4166 4847 5038 826
Size: 219 1986 1135 681 191 1471
Fragments arranged by size:
1986 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 2033 2166 2176 3010 1872
Size: 161 133 10 834 4545
Fragments arranged by size:
4545 834 161 133 10
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5683
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 3122 513
Size: 552 939 21 1097 3074
Fragments arranged by size:
3074 1097 939 552 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2295 3710 3727 3880 4065 4510 325
Size: 1255 715 1415 17 153 185 445 1498
Fragments arranged by size:
1498 1415 1255 715 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2661 2697 3929
Size: 274 80 33 51 1443 684 36 1232
Cuts at: 3929 5641 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1232 684 274 138 80 51
36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5683
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 2031 2164 2174 3008 1870
Size: 161 133 10 834 4545
Fragments arranged by size:
4545 834 161 133 10
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI
SwaI XcmI