Restriction map for
pFN21AE4323
(Circular) (Six-base) MAPSORT of: pFN21AE4323.seq Check: 3212 from: 1 to: 5683
pFN21AE4323 5683 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3493 278
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3026 1937
Size: 1089 4594
Acc65I G'GTAC_C
Cuts at: 3004 3004
Size: 5683
AclI AA'CG_TT
Cuts at: 1563 3810 4183 1563
Size: 2247 373 3063
Fragments arranged by size:
3063 2247 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3746 5130 5398 1632
Size: 2114 1384 268 1917
Fragments arranged by size:
2114 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5486
AflIII A'CryG_T
Cuts at: 1203 4582 5559 1203
Size: 3379 977 1327
Fragments arranged by size:
3379 1327 977
AgeI A'CCGG_T
Cuts at: 4514 4514
Size: 5683
AhdI GACnn_n'nnGTC
Cuts at: 4412 4412
Size: 5683
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5683
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5683
AlwNI CAG_nnn'CTG
Cuts at: 2787 2833 3426 4998 2787
Size: 46 593 1572 3472
Fragments arranged by size:
3472 1572 593 46
ApaI G_GGCC'C
Cuts at: 2258 2796 2258
Size: 538 5145
ApaLI G'TGCA_C
Cuts at: 3740 4896 3740
Size: 1156 4527
ApoI r'AATT_y
Cuts at: 1493 2992 3218 3370 5479 1493
Size: 1499 226 152 2109 1697
Fragments arranged by size:
2109 1697 1499 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4237 160
Size: 872 3205 1606
Fragments arranged by size:
3205 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2527 3008 1870
Size: 73 164 420 481 4545
Fragments arranged by size:
4545 481 420 164 73
BamHI G'GATC_C
Cuts at: 2837 3013 3380 2837
Size: 176 367 5140
Fragments arranged by size:
5140 367 176
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 2714 3004 4459 618
Size: 325 217 627 425 502 290 1455 1842
Fragments arranged by size:
1842 1455 627 502 425 325 290 217
BanII G_rGCy'C
Cuts at: 1391 2048 2105 2129 2258 2796 2885 3002 1391
Size: 657 57 24 129 538 89 117 4072
Fragments arranged by size:
4072 657 538 129 117 89 57 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2520 2890 928
Size: 971 621 370 3721
Fragments arranged by size:
3721 971 621 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3874 3908 1903
Size: 34 1937 34 3678
Fragments arranged by size:
3678 1937 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3575 4785 3575
Size: 1210 4473
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5683
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2169 2478 2616
Size: 107 122 71 631 605 497 309 138
Cuts at: 2616 2880 4294 136
Size: 264 1414 1525
Fragments arranged by size:
1525 1414 631 605 497 309 264 138
122 107 71
BglII A'GATC_T
Cuts at: 5678 5678
Size: 5683
BlpI GC'TnA_GC
Cuts at: 2042 3100 2042
Size: 1058 4625
Bme1580I G_kGCm'C
Cuts at: 1790 2058 2217 2258 2796 3744 4900 1790
Size: 268 159 41 538 948 1156 2573
Fragments arranged by size:
2573 1156 948 538 268 159 41
BmgBI CAC'GTC
Cuts at: 1318 2053 1318
Size: 735 4948
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2738 3414 4372
Size: 416 53 83 399 24 1321 676 958
Cuts at: 4372 442
Size: 1753
Fragments arranged by size:
1753 1321 958 676 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2137 2444 2617 2635 4343 1239
Size: 883 15 307 173 18 1708 2579
Fragments arranged by size:
2579 1708 883 307 173 18 15
Bpu10I CC'TnA_GC
Cuts at: 2123 2259 2123
Size: 136 5547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4673 4971 3808
Size: 865 298 4520
Fragments arranged by size:
4520 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2727 4346 882
Size: 617 1228 1619 2219
Fragments arranged by size:
2219 1619 1228 617
BsaAI yAC'GTr
Cuts at: 493 1204 3474 493
Size: 711 2270 2702
Fragments arranged by size:
2702 2270 711
BsaBI GATnn'nnATC
Cuts at: 5677 5677
Size: 5683
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3490 3872 4507 275
Size: 53 83 186 936 1957 382 635 1451
Fragments arranged by size:
1957 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4116 4514 4788 4935 5346 4116
Size: 398 274 147 411 4453
Fragments arranged by size:
4453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5458
Fragments arranged by size:
5458 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2095 1186
Size: 909 4774
BseYI C'CCAG_C
Cuts at: 2097 2208 2925 4886 5382 2097
Size: 111 717 1961 496 2398
Fragments arranged by size:
2398 1961 717 496 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5110
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3133 3894 4922 1540
Size: 275 186 1132 761 1028 2301
Fragments arranged by size:
2301 1132 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3744 3829 4900 3744
Size: 85 1071 4527
Fragments arranged by size:
4527 1071 85
BsmI GAATG_Cn'
Cuts at: 3194 3287 3194
Size: 93 5590
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2058 2105 2129 2217 2258 2796
Size: 399 258 10 47 24 88 41 538
Cuts at: 2796 2885 3002 3744 3829 4900 1391
Size: 89 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 258 117 89
88 85 47 41 24 10
BspEI T'CCGG_A
Cuts at: 5346 5346
Size: 5683
BspHI T'CATG_A
Cuts at: 3572 5302 3572
Size: 1730 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3038 844
Size: 2194 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2175 3570 1394
Size: 429 352 1395 3507
Fragments arranged by size:
3507 1395 429 352
BsrDI GCAATG_nn'
Cuts at: 65 1248 4178 4352 65
Size: 1183 2930 174 1396
Fragments arranged by size:
2930 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2615 4327 4514 1700
Size: 110 805 1712 187 2869
Fragments arranged by size:
2869 1712 805 187 110
BsrGI T'GTAC_A
Cuts at: 96 2824 96
Size: 2728 2955
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5683
BssSI C'ACGA_G
Cuts at: 3743 4755 3743
Size: 1012 4671
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5683
BstBI TT'CG_AA
Cuts at: 4487 4487
Size: 5683
BstEII G'GTnAC_C
Cuts at: 1184 2152 1184
Size: 968 4715
BstXI CCAn_nnnn'nTGG
Cuts at: 2188 2188
Size: 5683
BstYI r'GATC_y
Cuts at: 1972 2008 2837 3013 3380 3765 3782 5234 5320
Size: 36 829 176 367 385 17 1452 86
Cuts at: 5320 5332 5678 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 829 385 367 346 176 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2071 2386 2931 513
Size: 552 939 67 315 545 3265
Fragments arranged by size:
3265 939 552 545 315 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4944
Fragments arranged by size:
4944 683 56
BtsI GCAGTG_nn'
Cuts at: 2286 3199 3993 4013 2286
Size: 913 794 20 3956
Fragments arranged by size:
3956 913 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5683
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5648
DraI TTT'AAA
Cuts at: 2987 2987
Size: 5683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2894 4690 809
Size: 507 1578 1796 1802
Fragments arranged by size:
1802 1796 1578 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2170 2581 2613
Size: 54 1425 50 275 18 340 411 32
Cuts at: 2613 3130 4019 5648 8
Size: 517 889 1629 43
Fragments arranged by size:
1629 1425 889 517 411 340 275 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3130 1537
Size: 275 1318 4090
Fragments arranged by size:
4090 1318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2725 3613 1353
Size: 1372 888 3423
Fragments arranged by size:
3423 1372 888
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4270 4644 4790 387
Size: 1064 138 2681 374 146 1280
Fragments arranged by size:
2681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2137 2444 2617 2635 3746 4343
Size: 393 490 15 307 173 18 1111 597
Cuts at: 4343 5130 5398 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1111 787 597 490 393 307 268
173 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2189 2764 2189
Size: 575 5108
EcoO109I rG'GnC_Cy
Cuts at: 2111 2255 2674 2792 2793 2111
Size: 144 419 118 1 5001
Fragments arranged by size:
5001 419 144 118 1
EcoRI G'AATT_C
Cuts at: 2992 3370 2992
Size: 378 5305
FseI GG_CCGG'CC
Cuts at: 2619 2619
Size: 5683
FspI TGC'GCA
Cuts at: 4189 4189
Size: 5683
HaeII r_GCGC'y
Cuts at: 1678 2356 4830 1678
Size: 678 2474 2531
Fragments arranged by size:
2531 2474 678
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2940 2972 4324 4356 4398
Size: 32 135 32 1368 32 1352 32 42
Cuts at: 4398 4430 1373
Size: 32 2626
Fragments arranged by size:
2626 1368 1352 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3027 3273 669
Size: 988 281 1089 246 3079
Fragments arranged by size:
3079 1089 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5683
HpaI GTT'AAC
Cuts at: 3273 3273
Size: 5683
KpnI G_GTAC'C
Cuts at: 3008 3008
Size: 5683
MfeI C'AATT_G
Cuts at: 3282 3282
Size: 5683
MluI A'CGCG_T
Cuts at: 5559 5559
Size: 5683
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2091 2157 2312 2865 4797 4981 1305
Size: 786 66 155 553 1932 184 2007
Fragments arranged by size:
2007 1932 786 553 184 155 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3861
Fragments arranged by size:
3861 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3641 4000 4159 518
Size: 648 2475 359 159 2042
Fragments arranged by size:
2475 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2202 2239 2469 2865 3099 3776 4924 5169
Size: 596 37 230 396 234 677 1148 245
Cuts at: 5169 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 596 396 245 234 230
37
NaeI GCC'GGC
Cuts at: 2617 2617
Size: 5683
NcoI C'CATG_G
Cuts at: 513 1065 2004 2071 2386 513
Size: 552 939 67 315 3810
Fragments arranged by size:
3810 939 552 315 67
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5683
NgoMIV G'CCGG_C
Cuts at: 2615 2615
Size: 5683
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5683
NotI GC'GGCC_GC
Cuts at: 3130 3130
Size: 5683
NspI r_CATG'y
Cuts at: 1132 1229 3041 4586 1132
Size: 97 1812 1545 2229
Fragments arranged by size:
2229 1812 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2833 1348
Size: 1485 4198
PciI A'CATG_T
Cuts at: 4582 4582
Size: 5683
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2225 2392 2669 2932 1108
Size: 1117 167 277 263 3859
Fragments arranged by size:
3859 1117 277 263 167
PmeI GTTT'AAAC
Cuts at: 2987 2987
Size: 5683
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 5265 5297 3752
Size: 32 1481 32 4138
Fragments arranged by size:
4138 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 2674 2111
Size: 563 5120
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5683
PsiI TTA'TAA
Cuts at: 3253 3253
Size: 5683
PspOMI G'GGCC_C
Cuts at: 2254 2792 2254
Size: 538 5145
PstI C_TGCA'G
Cuts at: 830 3035 830
Size: 2205 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5683
PvuII CAG'CTG
Cuts at: 2202 2202
Size: 5683
SalI G'TCGA_C
Cuts at: 1936 3025 1936
Size: 1089 4594
SanDI GG'GwC_CC
Cuts at: 2111 2111
Size: 5683
SbfI CC_TGCA'GG
Cuts at: 3035 3035
Size: 5683
ScaI AGT'ACT
Cuts at: 3931 5643 3931
Size: 1712 3971
SfcI C'TryA_G
Cuts at: 826 1045 3031 4166 4847 5038 826
Size: 219 1986 1135 681 191 1471
Fragments arranged by size:
1986 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2616 2616
Size: 5683
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5683
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5683
SmaI CCC'GGG
Cuts at: 1872 2529 3010 1872
Size: 657 481 4545
Fragments arranged by size:
4545 657 481
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3787 4688 4950 820
Size: 197 926 1844 901 262 1553
Fragments arranged by size:
1844 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3474 493
Size: 2981 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5683
SphI G_CATG'C
Cuts at: 1132 3041 1132
Size: 1909 3774
SspI AAT'ATT
Cuts at: 5 52 3607 5
Size: 47 3555 2081
Fragments arranged by size:
3555 2081 47
StuI AGG'CCT
Cuts at: 1517 2610 1517
Size: 1093 4590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2071 2386 2796 3122 513
Size: 552 939 67 315 410 326 3074
Fragments arranged by size:
3074 939 552 410 326 315 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2200 2290 2789 3710 3727 3880 4065
Size: 1255 620 90 499 921 17 153 185
Cuts at: 4065 4510 325
Size: 445 1498
Fragments arranged by size:
1498 1255 921 620 499 445 185 153
90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2824 3929 5641
Size: 274 80 33 51 1443 847 1105 1712
Cuts at: 5641 96
Size: 138
Fragments arranged by size:
1712 1443 1105 847 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5643 5675 5643
Size: 32 5651
Tth111I GACn'n_nGTC
Cuts at: 1316 2083 1316
Size: 767 4916
XbaI T'CTAG_A
Cuts at: 3019 3019
Size: 5683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2384 2185
Size: 199 5484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5683
XmaI C'CCGG_G
Cuts at: 1870 2527 3008 1870
Size: 657 481 4545
Fragments arranged by size:
4545 657 481
XmnI GAAnn'nnTTC
Cuts at: 3812 3812
Size: 5683
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3491 276
Size: 53 83 186 936 1957 2468
Fragments arranged by size:
2468 1957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfoI SrfI SwaI