Restriction map for
pFN21AE4321
(Circular) (Six-base) MAPSORT of: pFN21AE4321.seq Check: 9828 from: 1 to: 5680
pFN21AE4321 5680 bp
With 182 enzymes: SgfI *
March 28, 2012 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2350 2350
Size: 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2913 3023 1937
Size: 976 110 4594
Fragments arranged by size:
4594 976 110
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2966 3743 5127 5395 1632
Size: 1334 777 1384 268 1917
Fragments arranged by size:
1917 1384 1334 777 268
AfeI AGC'GCT
Cuts at: 2193 2193
Size: 5680
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 2675 1166
Size: 1509 4171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2840 1184
Size: 1656 4024
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaI G_GGCC'C
Cuts at: 2048 2048
Size: 5680
ApaLI G'TGCA_C
Cuts at: 2815 3737 4893 2815
Size: 922 1156 3602
Fragments arranged by size:
3602 1156 922
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2065 2079 2105 2166 2559 3005 1870
Size: 73 122 14 26 61 393 446 4545
Fragments arranged by size:
4545 446 393 122 73 61 26 14
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2777 2902 3001 4456 618
Size: 325 217 627 990 125 99 1455 1842
Fragments arranged by size:
1842 1455 990 627 325 217 125 99
BanII G_rGCy'C
Cuts at: 1391 2048 2064 2874 2999 1391
Size: 657 16 810 125 4072
Fragments arranged by size:
4072 810 657 125 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2629 928
Size: 971 730 3979
Fragments arranged by size:
3979 971 730
BbvCI CC'TCA_GC
Cuts at: 2594 2594
Size: 5680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2371 2405 2837 2871 3871 3905 1903
Size: 34 434 34 432 34 1000 34 3678
Fragments arranged by size:
3678 1000 434 432 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2052 4291
Size: 107 122 71 631 605 337 43 2239
Cuts at: 4291 136
Size: 1525
Fragments arranged by size:
2239 1525 631 605 337 122 107 71
43
BglII A'GATC_T
Cuts at: 5675 5675
Size: 5680
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2080 2819 2907 3741 4897 1790
Size: 258 32 739 88 834 1156 2573
Fragments arranged by size:
2573 1156 834 739 258 88 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 4340 1239
Size: 1046 2055 2579
Fragments arranged by size:
2579 2055 1046
Bpu10I CC'TnA_GC
Cuts at: 2439 2594 2439
Size: 155 5525
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3805 4670 4968 2858
Size: 947 865 298 3570
Fragments arranged by size:
3570 947 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2298 2699 4343 882
Size: 617 799 401 1644 2219
Fragments arranged by size:
2219 1644 799 617 401
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2334 4113 4511 4785 4932 5343 2334
Size: 1779 398 274 147 411 2671
Fragments arranged by size:
2671 1779 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2624 2654 1178
Size: 30 165 30 1221 30 4204
Fragments arranged by size:
4204 1221 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2297 1186
Size: 1111 4569
BseYI C'CCAG_C
Cuts at: 2194 2279 2782 2829 4883 5379 2194
Size: 85 503 47 2054 496 2495
Fragments arranged by size:
2495 2054 503 496 85 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2113 2255 1163
Size: 573 377 142 4588
Fragments arranged by size:
4588 573 377 142
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2972 3130 3891 4919 1540
Size: 275 186 971 158 761 1028 2301
Fragments arranged by size:
2301 1028 971 761 275 186 158
BsiHKAI G_wGCw'C
Cuts at: 2819 2850 3741 3826 4897 2819
Size: 31 891 85 1071 3602
Fragments arranged by size:
3602 1071 891 85 31
BsmI GAATG_Cn'
Cuts at: 2239 3191 3284 2239
Size: 952 93 4635
Fragments arranged by size:
4635 952 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2064 2080 2819 2850 2874 2907
Size: 399 258 16 16 739 31 24 33
Cuts at: 2907 2999 3741 3826 4897 1391
Size: 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 739 399 258 92 85
33 31 24 16 16
BspEI T'CCGG_A
Cuts at: 2334 5343 2334
Size: 3009 2671
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 3035 844
Size: 1506 685 3489
Fragments arranged by size:
3489 1506 685
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2579 2727 4175 4349 65
Size: 1183 1331 148 1448 174 1396
Fragments arranged by size:
1448 1396 1331 1183 174 148
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 2070 2097 1925
Size: 145 27 5508
Fragments arranged by size:
5508 145 27
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2216 794
Size: 1422 4258
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2734 2263
Size: 471 5209
BstYI r'GATC_y
Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675
Size: 1038 367 385 17 1452 86 12 346
Cuts at: 5675 1972
Size: 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2130 2130
Size: 5680
BtgI C'CryG_G
Cuts at: 513 1065 2004 2048 2229 2288 2299 2733 513
Size: 552 939 44 181 59 11 434 3460
Fragments arranged by size:
3460 939 552 434 181 59 44 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 2681 3196 3990 4010 2142
Size: 539 515 794 20 3812
Fragments arranged by size:
3812 794 539 515 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5645
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DraIII CAC_nnn'GTG
Cuts at: 2410 2410
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2504 2719
Size: 54 1425 50 275 18 396 278 215
Cuts at: 2719 2969 3127 4016 5645 8
Size: 250 158 889 1629 43
Fragments arranged by size:
1629 1425 889 396 278 275 250 215
158 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2969 3127 1537
Size: 275 1157 158 4090
Fragments arranged by size:
4090 1157 275 158
EarI CTCTTCn'nnn_
Cuts at: 1353 2317 2942 3610 1353
Size: 964 625 668 3423
Fragments arranged by size:
3423 964 668 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2966 3743 4340 5127 5395 1239
Size: 393 653 681 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 681 653 597 393 268
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2195 4827 1678
Size: 517 2632 2531
Fragments arranged by size:
2632 2531 517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2878 2910 4321 4353 4395
Size: 32 135 32 1306 32 1411 32 42
Cuts at: 4395 4427 1373
Size: 32 2626
Fragments arranged by size:
2626 1411 1306 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2933 4794 4978 1305
Size: 1628 1861 184 2007
Fragments arranged by size:
2007 1861 1628 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2721 10
Size: 54 1425 343 889 2969
Fragments arranged by size:
2969 1425 889 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2622 2675 2732 3638 3997 4156 518
Size: 648 1456 53 57 906 359 159 2042
Fragments arranged by size:
2042 1456 906 648 359 159 57 53
MspA1I CmG'CkG
Cuts at: 1606 2015 2050 3096 3773 4921 5166 1606
Size: 409 35 1046 677 1148 245 2120
Fragments arranged by size:
2120 1148 1046 677 409 245 35
NcoI C'CATG_G
Cuts at: 513 1065 2004 2733 513
Size: 552 939 729 3460
Fragments arranged by size:
3460 939 729 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2203 3038 4583 1132
Size: 97 974 835 1545 2229
Fragments arranged by size:
2229 1545 974 835 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2383 2410 1108
Size: 1275 27 4378
Fragments arranged by size:
4378 1275 27
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PspOMI G'GGCC_C
Cuts at: 2044 2044
Size: 5680
PspXI vC'TCGA_Gb
Cuts at: 2559 2559
Size: 5680
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2015 2015
Size: 5680
SacII CC_GC'GG
Cuts at: 2051 2051
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SexAI A'CCwGG_T
Cuts at: 2406 2406
Size: 5680
SfcI C'TryA_G
Cuts at: 826 1045 3028 4163 4844 5035 826
Size: 219 1983 1135 681 191 1471
Fragments arranged by size:
1983 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 2067 2081 2107 3007 1872
Size: 195 14 26 900 4545
Fragments arranged by size:
4545 900 195 26 14
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2559 2873 3784 4685 4947 820
Size: 197 926 616 314 911 901 262 1553
Fragments arranged by size:
1553 926 911 901 616 314 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 2203 3038 1132
Size: 1071 835 3774
Fragments arranged by size:
3774 1071 835
SrfI GCCC'GGGC
Cuts at: 2107 2107
Size: 5680
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2316 2733 2805 2976 3119 513
Size: 552 939 312 417 72 171 143 3074
Fragments arranged by size:
3074 939 552 417 312 171 143 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2683 3707 3724 3877 4062 4507 325
Size: 1255 1103 1024 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1103 1024 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 2633 1316
Size: 1317 4363
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 2559 1943
Size: 616 5064
XmaI C'CCGG_G
Cuts at: 1870 2065 2079 2105 3005 1870
Size: 195 14 26 900 4545
Fragments arranged by size:
4545 900 195 26 14
XmnI GAAnn'nnTTC
Cuts at: 2516 3809 2516
Size: 1293 4387
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PsrI RsrII SacI SanDI SapI
SfiI SfoI SwaI XcmI