Restriction map for
pFN21AE4319
(Circular) (Six-base) MAPSORT of: pFN21AE4319.seq Check: 2910 from: 1 to: 5680
pFN21AE4319 5680 bp
With 182 enzymes: SgfI *
March 28, 2012 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2545 2545
Size: 5680
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 1937
Size: 1086 4594
Acc65I G'GTAC_C
Cuts at: 2063 3001 2063
Size: 938 4742
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 2801 3743 5127 5395 1632
Size: 572 597 942 1384 268 1917
Fragments arranged by size:
1917 1384 942 597 572 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 4409 4409
Size: 5680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2228 2889 1184
Size: 1044 661 3975
Fragments arranged by size:
3975 1044 661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2688 2720 2688
Size: 32 5648
AlwNI CAG_nnn'CTG
Cuts at: 2479 3423 4995 2479
Size: 944 1572 3164
Fragments arranged by size:
3164 1572 944
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2989 3215 3367 5476 1493
Size: 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 2267 3005 1870
Size: 73 274 50 738 4545
Fragments arranged by size:
4545 738 274 73 50
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2063 2212 3001 4456 618
Size: 325 217 627 276 149 789 1455 1842
Fragments arranged by size:
1842 1455 789 627 325 276 217 149
BanII G_rGCy'C
Cuts at: 1391 2648 2999 1391
Size: 1257 351 4072
Fragments arranged by size:
4072 1257 351
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2300 928
Size: 971 401 4308
Fragments arranged by size:
4308 971 401
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2643 4291
Size: 107 122 71 631 605 337 634 1648
Cuts at: 4291 136
Size: 1525
Fragments arranged by size:
1648 1525 634 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2745 5675 2745
Size: 2930 2750
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 3741 4897 1790
Size: 1951 1156 2573
Fragments arranged by size:
2573 1951 1156
BmgBI CAC'GTC
Cuts at: 1318 2194 1318
Size: 876 4804
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3411 4369 442
Size: 416 53 83 399 24 1994 958 1753
Fragments arranged by size:
1994 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2336 2465 2639 2711 2721 4340 1239
Size: 1055 42 129 174 72 10 1619 2579
Fragments arranged by size:
2579 1619 1055 174 129 72 42 10
Bpu10I CC'TnA_GC
Cuts at: 2114 2460 2753 2114
Size: 346 293 5041
Fragments arranged by size:
5041 346 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2612 3471 493
Size: 711 1408 859 2702
Fragments arranged by size:
2702 1408 859 711
BsaBI GATnn'nnATC
Cuts at: 2783 5674 2783
Size: 2891 2789
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3487 3869 4504 275
Size: 53 83 186 936 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2289 4113 4511 4785 4932 5343 2289
Size: 1824 398 274 147 411 2626
Fragments arranged by size:
2626 1824 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2213 2243 1178
Size: 30 165 30 810 30 4615
Fragments arranged by size:
4615 810 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2638 1186
Size: 1051 401 4228
Fragments arranged by size:
4228 1051 401
BseYI C'CCAG_C
Cuts at: 2479 4883 5379 2479
Size: 2404 496 2780
Fragments arranged by size:
2780 2404 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2012 2492 1163
Size: 573 276 480 4351
Fragments arranged by size:
4351 573 480 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3741 3826 4897 3741
Size: 85 1071 4524
Fragments arranged by size:
4524 1071 85
BsiWI C'GTAC_G
Cuts at: 2976 2976
Size: 5680
BsmI GAATG_Cn'
Cuts at: 2392 2567 3191 3284 2392
Size: 175 624 93 4788
Fragments arranged by size:
4788 624 175 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2648 2999 3741 3826 4897 1391
Size: 399 858 351 742 85 1071 2174
Fragments arranged by size:
2174 1071 858 742 399 351 85
BspEI T'CCGG_A
Cuts at: 2289 5343 2289
Size: 3054 2626
BspHI T'CATG_A
Cuts at: 3569 5299 3569
Size: 1730 3950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2545 2752 3035 844
Size: 1701 207 283 3489
Fragments arranged by size:
3489 1701 283 207
BsrBI CCG'CTC
Cuts at: 1394 1823 2362 3567 1394
Size: 429 539 1205 3507
Fragments arranged by size:
3507 1205 539 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2164 4175 4349 65
Size: 1183 916 2011 174 1396
Fragments arranged by size:
2011 1396 1183 916 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5680
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5680
BstXI CCAn_nnnn'nTGG
Cuts at: 2612 2612
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2745 3010 3377 3762 3779 5231 5317 5329
Size: 773 265 367 385 17 1452 86 12
Cuts at: 5329 5675 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 773 385 367 346 265 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 2599 3196 3990 4010 2599
Size: 597 794 20 4269
Fragments arranged by size:
4269 794 597 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2120 2155 529
Size: 35 1556 35 4054
Fragments arranged by size:
4054 1556 35 35
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2728 3127 4016
Size: 54 1425 50 275 18 898 399 889
Cuts at: 4016 5645 8
Size: 1629 43
Fragments arranged by size:
1629 1425 898 889 399 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2850 2921 3610 1353
Size: 713 784 71 689 3423
Fragments arranged by size:
3423 784 713 689 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2294 2336 2465 2639 2711 2721
Size: 393 572 90 42 129 174 72 10
Cuts at: 2721 2801 3743 4340 5127 5395 1239
Size: 80 942 597 787 268 1524
Fragments arranged by size:
1524 942 787 597 572 393 268 174
129 90 80 72 42 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 2690 2186
Size: 504 5176
EcoO109I rG'GnC_Cy
Cuts at: 2179 2179
Size: 5680
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2480 4827 1678
Size: 802 2347 2531
Fragments arranged by size:
2531 2347 802
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4321 4353 4395 4427 1373
Size: 32 135 32 2749 32 42 32 2626
Fragments arranged by size:
2749 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5680
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 2067 3005 2067
Size: 938 4742
MfeI C'AATT_G
Cuts at: 3279 3279
Size: 5680
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 4794 4978 1305
Size: 1209 2280 184 2007
Fragments arranged by size:
2280 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3858
Fragments arranged by size:
3858 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 3638 3997 4156 518
Size: 648 1662 810 359 159 2042
Fragments arranged by size:
2042 1662 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2099 2314 2546 2770 3096 3773 4921 5166
Size: 493 215 232 224 326 677 1148 245
Cuts at: 5166 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 493 326 245 232 224
215
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 3038 4583 1132
Size: 97 1809 1545 2229
Fragments arranged by size:
2229 1809 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5680
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2247 1108
Size: 1139 4541
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PmlI CAC'GTG
Cuts at: 2612 2612
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 3032 830
Size: 2202 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
PvuII CAG'CTG
Cuts at: 2099 2314 2546 2099
Size: 215 232 5233
Fragments arranged by size:
5233 232 215
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SfcI C'TryA_G
Cuts at: 826 1045 3028 4163 4844 5035 826
Size: 219 1983 1135 681 191 1471
Fragments arranged by size:
1983 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 3604 5
Size: 47 3552 2081
Fragments arranged by size:
3552 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2936 3119 513
Size: 552 939 932 183 3074
Fragments arranged by size:
3074 939 932 552 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 2583 3707 3724 3877 4062 4507
Size: 1255 852 151 1124 17 153 185 445
Cuts at: 4507 325
Size: 1498
Fragments arranged by size:
1498 1255 1124 852 445 185 153 151
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5680
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 3809 3809
Size: 5680
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI