Restriction map for
pFN21AE4318
(Circular) (Six-base) MAPSORT of: pFN21AE4318.seq Check: 4084 from: 1 to: 5677
pFN21AE4318 5677 bp
With 182 enzymes: SgfI *
March 28, 2012 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2222 2222
Size: 5677
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3487 278
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3020 1937
Size: 1083 4594
Acc65I G'GTAC_C
Cuts at: 2998 2998
Size: 5677
AclI AA'CG_TT
Cuts at: 1563 3804 4177 1563
Size: 2241 373 3063
Fragments arranged by size:
3063 2241 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2108 3740 5124 5392 1632
Size: 476 1632 1384 268 1917
Fragments arranged by size:
1917 1632 1384 476 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5480
AflIII A'CryG_T
Cuts at: 1203 4576 5553 1203
Size: 3373 977 1327
Fragments arranged by size:
3373 1327 977
AgeI A'CCGG_T
Cuts at: 4508 4508
Size: 5677
AhdI GACnn_n'nnGTC
Cuts at: 4406 4406
Size: 5677
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5677
AlwNI CAG_nnn'CTG
Cuts at: 2458 3420 4992 2458
Size: 962 1572 3143
Fragments arranged by size:
3143 1572 962
ApaI G_GGCC'C
Cuts at: 2791 2846 2791
Size: 55 5622
ApaLI G'TGCA_C
Cuts at: 2357 2642 3734 4890 2357
Size: 285 1092 1156 3144
Fragments arranged by size:
3144 1156 1092 285
ApoI r'AATT_y
Cuts at: 1493 2986 3212 3364 5473 1493
Size: 1493 226 152 2109 1697
Fragments arranged by size:
2109 1697 1493 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4231 160
Size: 872 3199 1606
Fragments arranged by size:
3199 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 3002 1870
Size: 73 657 402 4545
Fragments arranged by size:
4545 657 402 73
BamHI G'GATC_C
Cuts at: 3007 3374 3007
Size: 367 5310
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2998 4453 618
Size: 325 217 627 1211 1455 1842
Fragments arranged by size:
1842 1455 1211 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2124 2791 2831 2846 2861 2996 1391
Size: 733 667 40 15 15 135 4072
Fragments arranged by size:
4072 733 667 135 40 15 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4706
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2690 2724 3868 3902 1903
Size: 34 753 34 1144 34 3678
Fragments arranged by size:
3678 1144 753 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3569 4779 3569
Size: 1210 4467
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5677
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2510 4288 136
Size: 107 122 71 631 605 838 1778 1525
Fragments arranged by size:
1778 1525 838 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5672 5672
Size: 5677
BlpI GC'TnA_GC
Cuts at: 2144 3094 2144
Size: 950 4727
Bme1580I G_kGCm'C
Cuts at: 1790 2361 2646 2791 2846 3738 4894 1790
Size: 571 285 145 55 892 1156 2573
Fragments arranged by size:
2573 1156 892 571 285 145 55
BmgBI CAC'GTC
Cuts at: 1318 2575 1318
Size: 1257 4420
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2859 3408 4366
Size: 416 53 83 399 24 1442 549 958
Cuts at: 4366 442
Size: 1753
Fragments arranged by size:
1753 1442 958 549 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5677
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2153 2210 2537 2585 2845 4337 1239
Size: 914 57 327 48 260 1492 2579
Fragments arranged by size:
2579 1492 914 327 260 57 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 3802 4667 4965 2324
Size: 1478 865 298 3036
Fragments arranged by size:
3036 1478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4340 882
Size: 617 2841 2219
Fragments arranged by size:
2841 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3468 493
Size: 711 2264 2702
Fragments arranged by size:
2702 2264 711
BsaBI GATnn'nnATC
Cuts at: 5671 5671
Size: 5677
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2013 3484 3866 4501
Size: 53 83 186 936 480 1471 382 635
Cuts at: 4501 275
Size: 1451
Fragments arranged by size:
1471 1451 936 635 480 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2949 4110 4508 4782 4929 5340 2949
Size: 1161 398 274 147 411 3286
Fragments arranged by size:
3286 1161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2870 2899 2900 2929 1178
Size: 30 165 30 1467 29 1 29 3926
Fragments arranged by size:
3926 1467 165 30 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2630 2912 1186
Size: 978 466 282 3951
Fragments arranged by size:
3951 978 466 282
BseYI C'CCAG_C
Cuts at: 2535 2741 4880 5376 2535
Size: 206 2139 496 2836
Fragments arranged by size:
2836 2139 496 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2834 1163
Size: 573 1098 4006
Fragments arranged by size:
4006 1098 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2700 3127 3888 4916 1540
Size: 275 186 699 427 761 1028 2301
Fragments arranged by size:
2301 1028 761 699 427 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 2340 2361 2448 2646 2753 2861 3738 3823
Size: 216 21 87 198 107 108 877 85
Cuts at: 3823 4894 2124
Size: 1071 2907
Fragments arranged by size:
2907 1071 877 216 198 108 107 87
85 21
BsmI GAATG_Cn'
Cuts at: 2273 2660 3188 3281 2273
Size: 387 528 93 4669
Fragments arranged by size:
4669 528 387 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2861 2861
Size: 5677
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2340 2361 2448 2646 2753 2791
Size: 399 334 216 21 87 198 107 38
Cuts at: 2791 2831 2846 2861 2996 3738 3823 4894 1391
Size: 40 15 15 135 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 216 198 135
107 87 85 40 38 21 15 15
BspEI T'CCGG_A
Cuts at: 5340 5340
Size: 5677
BspHI T'CATG_A
Cuts at: 3566 5296 3566
Size: 1730 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2222 2325 2712 3032 844
Size: 1378 103 387 320 3489
Fragments arranged by size:
3489 1378 387 320 103
BsrBI CCG'CTC
Cuts at: 1394 1823 2569 2605 3564 1394
Size: 429 746 36 959 3507
Fragments arranged by size:
3507 959 746 429 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 2277 4172 4346 65
Size: 1183 1029 1895 174 1396
Fragments arranged by size:
1895 1396 1183 1029 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4321 4508 1700
Size: 110 2511 187 2869
Fragments arranged by size:
2869 2511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5677
BssSI C'ACGA_G
Cuts at: 2639 3737 4749 2639
Size: 1098 1012 3567
Fragments arranged by size:
3567 1098 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5677
BstBI TT'CG_AA
Cuts at: 4481 4481
Size: 5677
BstEII G'GTnAC_C
Cuts at: 1184 2291 1184
Size: 1107 4570
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 5677
BstYI r'GATC_y
Cuts at: 1972 3007 3374 3759 3776 5228 5314 5326 5672
Size: 1035 367 385 17 1452 86 12 346
Cuts at: 5672 1972
Size: 1977
Fragments arranged by size:
1977 1452 1035 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2895 2895
Size: 5677
BtgI C'CryG_G
Cuts at: 513 1065 2223 2505 2780 2838 513
Size: 552 1158 282 275 58 3352
Fragments arranged by size:
3352 1158 552 282 275 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4938
Fragments arranged by size:
4938 683 56
BtsI GCAGTG_nn'
Cuts at: 2501 3193 3987 4007 2501
Size: 692 794 20 4171
Fragments arranged by size:
4171 794 692 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5677
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5642
DraI TTT'AAA
Cuts at: 2981 2981
Size: 5677
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4684 809
Size: 507 3368 1802
Fragments arranged by size:
3368 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2697 3124
Size: 54 1425 50 275 18 192 675 427
Cuts at: 3124 4013 5642 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 675 427 275 192 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2697 3124 1537
Size: 275 885 427 4090
Fragments arranged by size:
4090 885 427 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2067 2112 3607 1353
Size: 698 16 45 1495 3423
Fragments arranged by size:
3423 1495 698 45 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2338 4264 4638 4784 387
Size: 1064 138 749 1926 374 146 1280
Fragments arranged by size:
1926 1280 1064 749 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2859 2122
Size: 737 4940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2153 2210 2537 2585 2845 3740
Size: 393 476 45 57 327 48 260 895
Cuts at: 3740 4337 5124 5392 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 895 787 597 476 393 327 268
260 57 48 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2726 2894 2726
Size: 168 5509
EcoO109I rG'GnC_Cy
Cuts at: 2041 2314 2512 2620 2731 2787 2041
Size: 273 198 108 111 56 4931
Fragments arranged by size:
4931 273 198 111 108 56
EcoRI G'AATT_C
Cuts at: 2986 3364 2986
Size: 378 5299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 5677
FspI TGC'GCA
Cuts at: 4183 4183
Size: 5677
HaeII r_GCGC'y
Cuts at: 1678 2564 4824 1678
Size: 886 2260 2531
Fragments arranged by size:
2531 2260 886
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2870 2902 4318 4350 4392
Size: 32 135 32 1298 32 1416 32 42
Cuts at: 4392 4424 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 1298 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2590 3021 3267 669
Size: 988 281 652 431 246 3079
Fragments arranged by size:
3079 988 652 431 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5677
HpaI GTT'AAC
Cuts at: 3267 3267
Size: 5677
KpnI G_GTAC'C
Cuts at: 3002 3002
Size: 5677
MfeI C'AATT_G
Cuts at: 3276 3276
Size: 5677
MluI A'CGCG_T
Cuts at: 5553 5553
Size: 5677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4791 4975 1305
Size: 3486 184 2007
Fragments arranged by size:
3486 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 10
Size: 54 1425 343 192 3663
Fragments arranged by size:
3663 1425 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 2810 3635 3994 4153 518
Size: 648 1017 627 825 359 159 2042
Fragments arranged by size:
2042 1017 825 648 627 359 159
MspA1I CmG'CkG
Cuts at: 1606 2437 2782 3093 3770 4918 5163 1606
Size: 831 345 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 831 677 345 311 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5125
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5677
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5677
NotI GC'GGCC_GC
Cuts at: 3124 3124
Size: 5677
NspI r_CATG'y
Cuts at: 1132 1229 2630 3035 4580 1132
Size: 97 1401 405 1545 2229
Fragments arranged by size:
2229 1545 1401 405 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5677
PciI A'CATG_T
Cuts at: 4576 4576
Size: 5677
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5677
PmeI GTTT'AAAC
Cuts at: 2981 2981
Size: 5677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 5259 5291 3746
Size: 32 1481 32 4132
Fragments arranged by size:
4132 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2041 2314 2731 2041
Size: 273 417 4987
Fragments arranged by size:
4987 417 273
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5677
PsiI TTA'TAA
Cuts at: 3247 3247
Size: 5677
PspOMI G'GGCC_C
Cuts at: 2787 2842 2787
Size: 55 5622
PstI C_TGCA'G
Cuts at: 830 2866 3029 830
Size: 2036 163 3478
Fragments arranged by size:
3478 2036 163
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5677
PvuII CAG'CTG
Cuts at: 2437 2437
Size: 5677
SacI G_AGCT'C
Cuts at: 2124 2861 2124
Size: 737 4940
SacII CC_GC'GG
Cuts at: 2783 2783
Size: 5677
SalI G'TCGA_C
Cuts at: 1936 3019 1936
Size: 1083 4594
SapI GCTCTTCn'nnn_
Cuts at: 2112 2112
Size: 5677
SbfI CC_TGCA'GG
Cuts at: 3029 3029
Size: 5677
ScaI AGT'ACT
Cuts at: 2903 3925 5637 2903
Size: 1022 1712 2943
Fragments arranged by size:
2943 1712 1022
SfcI C'TryA_G
Cuts at: 826 1045 2470 2862 3025 4160 4841 5032 826
Size: 219 1425 392 163 1135 681 191 1471
Fragments arranged by size:
1471 1425 1135 681 392 219 191 163
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5677
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5677
SmaI CCC'GGG
Cuts at: 1872 3004 1872
Size: 1132 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 3781 4682 4944 820
Size: 197 926 396 1442 901 262 1553
Fragments arranged by size:
1553 1442 926 901 396 262 197
SnaBI TAC'GTA
Cuts at: 493 3468 493
Size: 2975 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5677
SphI G_CATG'C
Cuts at: 1132 2630 3035 1132
Size: 1498 405 3774
Fragments arranged by size:
3774 1498 405
SspI AAT'ATT
Cuts at: 5 52 3601 5
Size: 47 3549 2081
Fragments arranged by size:
3549 2081 47
StuI AGG'CCT
Cuts at: 1517 2822 1517
Size: 1305 4372
StyI C'CwwG_G
Cuts at: 513 1065 3116 513
Size: 552 2051 3074
Fragments arranged by size:
3074 2051 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3704 3721 3874 4059 4504 325
Size: 1255 2124 17 153 185 445 1498
Fragments arranged by size:
2124 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 3923 5635
Size: 274 80 33 51 1443 924 1022 1712
Cuts at: 5635 96
Size: 138
Fragments arranged by size:
1712 1443 1022 924 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2899 2931 5637 5669 2899
Size: 32 2706 32 2907
Fragments arranged by size:
2907 2706 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5677
XbaI T'CTAG_A
Cuts at: 3013 3013
Size: 5677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2185
Size: 5677
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5677
XmaI C'CCGG_G
Cuts at: 1870 3002 1870
Size: 1132 4545
XmnI GAAnn'nnTTC
Cuts at: 3806 3806
Size: 5677
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3485 276
Size: 53 83 186 936 1951 2468
Fragments arranged by size:
2468 1951 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI