Restriction map for
pFN21AE4314
(Circular) (Six-base) MAPSORT of: pFN21AE4314.seq Check: 9540 from: 1 to: 5674
pFN21AE4314 5674 bp
With 182 enzymes: SgfI *
March 28, 2012 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2839 2839
Size: 5674
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2995 2995
Size: 5674
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2856 3737 5121 5389 1632
Size: 1224 881 1384 268 1917
Fragments arranged by size:
1917 1384 1224 881 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 2161 4573 5550 1203
Size: 958 2412 977 1327
Fragments arranged by size:
2412 1327 977 958
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 4403 4403
Size: 5674
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 2366 3417 4989 2366
Size: 1051 1572 3051
Fragments arranged by size:
3051 1572 1051
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2858 2983 3209 3361 5470 1493
Size: 1365 125 226 152 2109 1697
Fragments arranged by size:
2109 1697 1365 226 152 125
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 1870
Size: 73 1056 4545
Fragments arranged by size:
4545 1056 73
BamHI G'GATC_C
Cuts at: 2663 3004 3371 2663
Size: 341 367 4966
Fragments arranged by size:
4966 367 341
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2995 4450 618
Size: 325 217 627 282 926 1455 1842
Fragments arranged by size:
1842 1455 926 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2550 2993 1391
Size: 1159 443 4072
Fragments arranged by size:
4072 1159 443
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 928
Size: 971 342 4361
Fragments arranged by size:
4361 971 342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3865 3899 1903
Size: 34 1928 34 3678
Fragments arranged by size:
3678 1928 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 3735 4891 1790
Size: 1945 1156 2573
Fragments arranged by size:
2573 1945 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2714 3405 4363
Size: 416 53 83 399 24 1297 691 958
Cuts at: 4363 442
Size: 1753
Fragments arranged by size:
1753 1297 958 691 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2543 4334 1239
Size: 1304 1791 2579
Fragments arranged by size:
2579 1791 1304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 3799 4664 4962 2534
Size: 1265 865 298 3246
Fragments arranged by size:
3246 1265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4337 882
Size: 617 2838 2219
Fragments arranged by size:
2838 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3465 493
Size: 711 2261 2702
Fragments arranged by size:
2702 2261 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3481 3863 4498 275
Size: 53 83 186 936 1948 382 635 1451
Fragments arranged by size:
1948 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4107 4505 4779 4926 5337 4107
Size: 398 274 147 411 4444
Fragments arranged by size:
4444 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 1178
Size: 30 165 30 1278 30 4141
Fragments arranged by size:
4141 1278 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2891 1186
Size: 1006 699 3969
Fragments arranged by size:
3969 1006 699
BseYI C'CCAG_C
Cuts at: 2758 4877 5373 2758
Size: 2119 496 3059
Fragments arranged by size:
3059 2119 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 2867 1163
Size: 573 406 725 3970
Fragments arranged by size:
3970 725 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2659 2709 3735 3820 4891 2659
Size: 50 1026 85 1071 3442
Fragments arranged by size:
3442 1071 1026 85 50
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2550 2659 2709 2993 3735 3820 4891
Size: 399 760 109 50 284 742 85 1071
Cuts at: 4891 1391
Size: 2174
Fragments arranged by size:
2174 1071 760 742 399 284 109 85
50
BspEI T'CCGG_A
Cuts at: 5337 5337
Size: 5674
BspHI T'CATG_A
Cuts at: 3563 5293 3563
Size: 1730 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2173 2839 3029 844
Size: 1329 666 190 3489
Fragments arranged by size:
3489 1329 666 190
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4169 4343 65
Size: 1183 2921 174 1396
Fragments arranged by size:
2921 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 3734 4746 3734
Size: 1012 4662
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 2012 4478 2012
Size: 2466 3208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2431 2663 3004 3371 3756 3773 5225 5311
Size: 459 232 341 367 385 17 1452 86
Cuts at: 5311 5323 5669 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 459 385 367 346 341 232
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2454 2681 513
Size: 552 939 450 227 3506
Fragments arranged by size:
3506 939 552 450 227
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 2227 3190 3984 4004 2227
Size: 963 794 20 3897
Fragments arranged by size:
3897 963 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2193 2481 2808
Size: 54 1425 50 275 18 363 288 327
Cuts at: 2808 3121 4010 5639 8
Size: 313 889 1629 43
Fragments arranged by size:
1629 1425 889 363 327 313 288 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 3604 1353
Size: 869 1382 3423
Fragments arranged by size:
3423 1382 869
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2543 2856 3737 4334 5121 5389 1239
Size: 393 911 313 881 597 787 268 1524
Fragments arranged by size:
1524 911 881 787 597 393 313 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2302 2302
Size: 5674
EcoO109I rG'GnC_Cy
Cuts at: 2716 2716
Size: 5674
EcoRI G'AATT_C
Cuts at: 2858 2983 3361 2858
Size: 125 378 5171
Fragments arranged by size:
5171 378 125
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 4821 1678
Size: 3143 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2181 2213 2681 2713 4315
Size: 32 135 32 609 32 468 32 1602
Cuts at: 4315 4347 4389 4421 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1602 609 468 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 2868 3018 3264 669
Size: 988 281 400 530 150 246 3079
Fragments arranged by size:
3079 988 530 400 281 246 150
HindIII A'AGCT_T
Cuts at: 748 2255 748
Size: 1507 4167
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KpnI G_GTAC'C
Cuts at: 2999 2999
Size: 5674
MfeI C'AATT_G
Cuts at: 2324 3273 2324
Size: 949 4725
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2167 2314 2526 4788 4972 1305
Size: 862 147 212 2262 184 2007
Fragments arranged by size:
2262 2007 862 212 184 147
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2483 2810 10
Size: 54 1425 343 363 288 327 2874
Fragments arranged by size:
2874 1425 363 343 327 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3632 3991 4150 518
Size: 648 1329 1137 359 159 2042
Fragments arranged by size:
2042 1329 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2785 3090 3767 4915 5160 1606
Size: 1179 305 677 1148 245 2120
Fragments arranged by size:
2120 1179 1148 677 305 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2454 2681 513
Size: 552 939 450 227 3506
Fragments arranged by size:
3506 939 552 450 227
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 2165 3032 4577 1132
Size: 97 936 867 1545 2229
Fragments arranged by size:
2229 1545 936 867 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 2161 4573 2161
Size: 2412 3262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2716 2716
Size: 5674
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PstI C_TGCA'G
Cuts at: 830 2086 2790 3026 830
Size: 1256 704 236 3478
Fragments arranged by size:
3478 1256 704 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
PvuII CAG'CTG
Cuts at: 2785 2785
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 3922 5634 3922
Size: 1712 3962
SexAI A'CCwGG_T
Cuts at: 2571 2571
Size: 5674
SfcI C'TryA_G
Cuts at: 826 1045 2082 2650 2786 3022 4157 4838 5029
Size: 219 1037 568 136 236 1135 681 191
Cuts at: 5029 826
Size: 1471
Fragments arranged by size:
1471 1135 1037 681 568 236 219 191
136
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 3001 1872
Size: 1129 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2549 3778 4679 4941 820
Size: 197 926 606 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 606 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 2298 2454 2681 2733 3113 513
Size: 552 939 294 156 227 52 380 3074
Fragments arranged by size:
3074 939 552 380 294 227 156 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2674 3701 3718 3871 4056 4501 325
Size: 1255 1094 1027 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1094 1027 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2553 3920 5632
Size: 274 80 33 51 1443 576 1367 1712
Cuts at: 5632 96
Size: 138
Fragments arranged by size:
1712 1443 1367 576 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 2720 1316
Size: 1404 4270
XbaI T'CTAG_A
Cuts at: 2107 3010 2107
Size: 903 4771
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2789 2789
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2999 1870
Size: 1129 4545
XmnI GAAnn'nnTTC
Cuts at: 3803 3803
Size: 5674
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI