Restriction map for
pFN21AE4313
(Circular) (Six-base) MAPSORT of: pFN21AE4313.seq Check: 822 from: 1 to: 5671
pFN21AE4313 5671 bp
With 182 enzymes: SgfI *
March 28, 2012 14:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 3014 1937
Size: 705 372 4594
Fragments arranged by size:
4594 705 372
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2985 3734 5118 5386 1632
Size: 370 983 749 1384 268 1917
Fragments arranged by size:
1917 1384 983 749 370 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 2132 1166
Size: 966 4705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5671
AlwNI CAG_nnn'CTG
Cuts at: 2158 2651 3414 4986 2158
Size: 493 763 1572 2843
Fragments arranged by size:
2843 1572 763 493
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2161 2486 2980 3206 3358 5467 1493
Size: 668 325 494 226 152 2109 1697
Fragments arranged by size:
2109 1697 668 494 325 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2992 4447 618
Size: 325 217 627 1205 1455 1842
Fragments arranged by size:
1842 1455 1205 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2990 1391
Size: 1599 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 3732 4888 1790
Size: 1942 1156 2573
Fragments arranged by size:
2573 1942 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3402 4360 442
Size: 416 53 83 399 24 1985 958 1753
Fragments arranged by size:
1985 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2247 1056
Size: 1191 4480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2550 4331 1239
Size: 1311 1781 2579
Fragments arranged by size:
2579 1781 1311
Bpu10I CC'TnA_GC
Cuts at: 2175 2175
Size: 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2539 2542 2569 2572 1178
Size: 30 165 30 1136 3 27 3 4277
Fragments arranged by size:
4277 1136 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2547 2729 2873 1186
Size: 1361 182 144 3984
Fragments arranged by size:
3984 1361 182 144
BseYI C'CCAG_C
Cuts at: 2194 2268 2696 4874 5370 2194
Size: 74 428 2178 496 2495
Fragments arranged by size:
2495 2178 496 428 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2103 1163
Size: 573 367 4731
Fragments arranged by size:
4731 573 367
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3732 3817 4888 3732
Size: 85 1071 4515
Fragments arranged by size:
4515 1071 85
BsmI GAATG_Cn'
Cuts at: 2421 2678 3182 3275 2421
Size: 257 504 93 4817
Fragments arranged by size:
4817 504 257 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2990 3732 3817 4888 1391
Size: 399 1200 742 85 1071 2174
Fragments arranged by size:
2174 1200 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2548 3026 844
Size: 1704 478 3489
Fragments arranged by size:
3489 1704 478
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2210 4315 4502 1700
Size: 110 400 2105 187 2869
Fragments arranged by size:
2869 2105 400 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 2053 2416 3731 4743 2053
Size: 363 1315 1012 2981
Fragments arranged by size:
2981 1315 1012 363
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2446 3001 3368 3753 3770 5222 5308 5320
Size: 474 555 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 555 474 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2585 2715 513
Size: 552 939 581 130 3469
Fragments arranged by size:
3469 939 581 552 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2965 487
Size: 683 56 1739 3193
Fragments arranged by size:
3193 1739 683 56
BtsI GCAGTG_nn'
Cuts at: 3187 3981 4001 3187
Size: 794 20 4857
Fragments arranged by size:
4857 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2208 3118
Size: 54 1425 50 275 18 177 201 910
Cuts at: 3118 4007 5636 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 275 201 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 2747 2913 3601 1353
Size: 729 665 166 688 3423
Fragments arranged by size:
3423 729 688 665 166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2550 2985 3734 4331 5118 5386
Size: 393 370 548 435 749 597 787 268
Cuts at: 5386 1239
Size: 1524
Fragments arranged by size:
1524 787 749 597 548 435 393 370
268
EcoRI G'AATT_C
Cuts at: 2980 3358 2980
Size: 378 5293
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 4818 1678
Size: 3140 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2274 2306 4312 4344 4386
Size: 32 135 32 702 32 2006 32 42
Cuts at: 4386 4418 1373
Size: 32 2626
Fragments arranged by size:
2626 2006 702 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3015 3261 669
Size: 988 281 1077 246 3079
Fragments arranged by size:
3079 1077 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2254 4785 4969 1305
Size: 949 2531 184 2007
Fragments arranged by size:
2531 2007 949 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3672
Fragments arranged by size:
3672 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2102 2132 3629 3988 4147 518
Size: 648 936 30 1497 359 159 2042
Fragments arranged by size:
2042 1497 936 648 359 159 30
MspA1I CmG'CkG
Cuts at: 1606 2158 2759 3087 3764 4912 5157 1606
Size: 552 601 328 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 601 552 328 245
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 5671
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4180
Fragments arranged by size:
4180 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5671
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 5671
NheI G'CTAG_C
Cuts at: 1052 2243 1052
Size: 1191 4480
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 3029 4574 1132
Size: 97 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PstI C_TGCA'G
Cuts at: 830 2758 3023 830
Size: 1928 265 3478
Fragments arranged by size:
3478 1928 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
PvuII CAG'CTG
Cuts at: 2158 2759 2158
Size: 601 5070
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SapI GCTCTTCn'nnn_
Cuts at: 2082 2082
Size: 5671
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2185 2643 2754 3019 4154 4835 5026
Size: 219 1140 458 111 265 1135 681 191
Cuts at: 5026 826
Size: 1471
Fragments arranged by size:
1471 1140 1135 681 458 265 219 191
111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3775 4676 4938 820
Size: 197 926 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 2183 2798 1517
Size: 666 615 4390
Fragments arranged by size:
4390 666 615
StyI C'CwwG_G
Cuts at: 513 1065 2004 2068 3110 513
Size: 552 939 64 1042 3074
Fragments arranged by size:
3074 1042 939 552 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 3698 3715 3868 4053 4498 325
Size: 1255 747 1371 17 153 185 445 1498
Fragments arranged by size:
1498 1371 1255 747 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3917 5629 96
Size: 274 80 33 51 1443 1940 1712 138
Fragments arranged by size:
1940 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2431 2463 2566 2598 5631 5663 2431
Size: 32 103 32 3033 32 2439
Fragments arranged by size:
3033 2439 103 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5671
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 2798 3800 2798
Size: 1002 4669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI