Restriction map for
pFN21AE4312
(Circular) (Six-base) MAPSORT of: pFN21AE4312.seq Check: 955 from: 1 to: 5671
pFN21AE4312 5671 bp
With 182 enzymes: SgfI *
March 28, 2012 14:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3481 278
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 1937
Size: 1077 4594
Acc65I G'GTAC_C
Cuts at: 2992 2992
Size: 5671
AclI AA'CG_TT
Cuts at: 1563 3798 4171 1563
Size: 2235 373 3063
Fragments arranged by size:
3063 2235 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2538 3734 5118 5386 1632
Size: 906 1196 1384 268 1917
Fragments arranged by size:
1917 1384 1196 906 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5474
AflIII A'CryG_T
Cuts at: 1203 4570 5547 1203
Size: 3367 977 1327
Fragments arranged by size:
3367 1327 977
AgeI A'CCGG_T
Cuts at: 4502 4502
Size: 5671
AhdI GACnn_n'nnGTC
Cuts at: 4400 4400
Size: 5671
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2749 1184
Size: 1565 4106
AlwNI CAG_nnn'CTG
Cuts at: 3414 4986 3414
Size: 1572 4099
ApaLI G'TGCA_C
Cuts at: 3728 4884 3728
Size: 1156 4515
ApoI r'AATT_y
Cuts at: 1493 2095 2267 2631 2980 3206 3358 5467 1493
Size: 602 172 364 349 226 152 2109 1697
Fragments arranged by size:
2109 1697 602 364 349 226 172 152
AseI AT'TA_AT
Cuts at: 160 1032 4225 160
Size: 872 3193 1606
Fragments arranged by size:
3193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
AvaI C'yCGr_G
Cuts at: 1870 1943 2996 1870
Size: 73 1053 4545
Fragments arranged by size:
4545 1053 73
BamHI G'GATC_C
Cuts at: 3001 3368 3001
Size: 367 5304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2992 4447 618
Size: 325 217 627 1205 1455 1842
Fragments arranged by size:
1842 1455 1205 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2990 1391
Size: 1599 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4700
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3862 3896 1903
Size: 34 1925 34 3678
Fragments arranged by size:
3678 1925 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3563 4773 3563
Size: 1210 4461
BclI T'GATC_A
Cuts at: 1548 2313 1548
Size: 765 4906
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4282 136
Size: 107 122 71 631 605 2610 1525
Fragments arranged by size:
2610 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5666 5666
Size: 5671
BlpI GC'TnA_GC
Cuts at: 3088 3088
Size: 5671
Bme1580I G_kGCm'C
Cuts at: 1790 3732 4888 1790
Size: 1942 1156 2573
Fragments arranged by size:
2573 1942 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5671
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2336 2461 2837
Size: 416 53 83 399 24 919 125 376
Cuts at: 2837 3402 4360 442
Size: 565 958 1753
Fragments arranged by size:
1753 958 919 565 416 399 376 125
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2106 4331 1239
Size: 867 2225 2579
Fragments arranged by size:
2579 2225 867
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3796 4661 4959 3796
Size: 865 298 4508
Fragments arranged by size:
4508 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4334 882
Size: 617 2835 2219
Fragments arranged by size:
2835 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3462 493
Size: 711 2258 2702
Fragments arranged by size:
2702 2258 711
BsaBI GATnn'nnATC
Cuts at: 5665 5665
Size: 5671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3478 3860 4495 275
Size: 53 83 186 936 1945 382 635 1451
Fragments arranged by size:
1945 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4104 4502 4776 4923 5334 4104
Size: 398 274 147 411 4441
Fragments arranged by size:
4441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5446
Fragments arranged by size:
5446 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2340 2886 1186
Size: 1154 546 3971
Fragments arranged by size:
3971 1154 546
BseYI C'CCAG_C
Cuts at: 4874 5370 4874
Size: 496 5175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5098
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 3882 4910 1540
Size: 275 186 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3732 3817 4888 3732
Size: 85 1071 4515
Fragments arranged by size:
4515 1071 85
BsmI GAATG_Cn'
Cuts at: 3182 3275 3182
Size: 93 5578
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2990 3732 3817 4888 1391
Size: 399 1200 742 85 1071 2174
Fragments arranged by size:
2174 1200 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5334 5334
Size: 5671
BspHI T'CATG_A
Cuts at: 3560 5290 3560
Size: 1730 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3026 844
Size: 2182 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3558 1394
Size: 429 1735 3507
Fragments arranged by size:
3507 1735 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4166 4340 65
Size: 1183 2918 174 1396
Fragments arranged by size:
2918 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4315 4502 1700
Size: 110 2505 187 2869
Fragments arranged by size:
2869 2505 187 110
BsrGI T'GTAC_A
Cuts at: 96 2596 96
Size: 2500 3171
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5671
BssSI C'ACGA_G
Cuts at: 3731 4743 3731
Size: 1012 4659
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5671
BstBI TT'CG_AA
Cuts at: 4475 4475
Size: 5671
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5671
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2626
Size: 5671
BstYI r'GATC_y
Cuts at: 1972 2157 3001 3368 3753 3770 5222 5308 5320
Size: 185 844 367 385 17 1452 86 12
Cuts at: 5320 5666 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 844 385 367 346 185 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2018 513
Size: 552 953 4166
Fragments arranged by size:
4166 953 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4932
Fragments arranged by size:
4932 683 56
BtsI GCAGTG_nn'
Cuts at: 2178 3187 3981 4001 2178
Size: 1009 794 20 3848
Fragments arranged by size:
3848 1009 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5636
DraI TTT'AAA
Cuts at: 2975 2975
Size: 5671
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4678 809
Size: 507 3362 1802
Fragments arranged by size:
3362 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 3118 4007
Size: 54 1425 50 275 18 750 538 889
Cuts at: 4007 5636 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 750 538 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 1537
Size: 275 1306 4090
Fragments arranged by size:
4090 1306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2017 3601 1353
Size: 664 1584 3423
Fragments arranged by size:
3423 1584 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4258 4632 4778 387
Size: 1064 138 2669 374 146 1280
Fragments arranged by size:
2669 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2538 3734 4331 5118 5386 1239
Size: 393 474 432 1196 597 787 268 1524
Fragments arranged by size:
1524 1196 787 597 474 432 393 268
EcoRI G'AATT_C
Cuts at: 2095 2980 3358 2095
Size: 885 378 4408
Fragments arranged by size:
4408 885 378
FspI TGC'GCA
Cuts at: 4177 4177
Size: 5671
HaeII r_GCGC'y
Cuts at: 1678 2949 4818 1678
Size: 1271 1869 2531
Fragments arranged by size:
2531 1869 1271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4312 4344 4386 4418 1373
Size: 32 135 32 2740 32 42 32 2626
Fragments arranged by size:
2740 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 3015 3261 669
Size: 988 281 367 710 246 3079
Fragments arranged by size:
3079 988 710 367 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5671
HpaI GTT'AAC
Cuts at: 3261 3261
Size: 5671
KpnI G_GTAC'C
Cuts at: 2996 2996
Size: 5671
MfeI C'AATT_G
Cuts at: 3270 3270
Size: 5671
MluI A'CGCG_T
Cuts at: 5547 5547
Size: 5671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4785 4969 1305
Size: 3480 184 2007
Fragments arranged by size:
3480 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 10
Size: 54 1425 343 750 3099
Fragments arranged by size:
3099 1425 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3629 3988 4147 518
Size: 648 2463 359 159 2042
Fragments arranged by size:
2463 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3087 3764 4912 5157 1606
Size: 1481 677 1148 245 2120
Fragments arranged by size:
2120 1481 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5119
NdeI CA'TA_TG
Cuts at: 387 2382 2884 387
Size: 1995 502 3174
Fragments arranged by size:
3174 1995 502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5671
NotI GC'GGCC_GC
Cuts at: 3118 3118
Size: 5671
NspI r_CATG'y
Cuts at: 1132 1229 2187 2303 3029 4574 1132
Size: 97 958 116 726 1545 2229
Fragments arranged by size:
2229 1545 958 726 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5671
PciI A'CATG_T
Cuts at: 4570 4570
Size: 5671
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5671
PmeI GTTT'AAAC
Cuts at: 2975 2975
Size: 5671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 5253 5285 3740
Size: 32 1481 32 4126
Fragments arranged by size:
4126 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5671
PsiI TTA'TAA
Cuts at: 3241 3241
Size: 5671
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2453 2485 2453
Size: 32 5639
PstI C_TGCA'G
Cuts at: 830 2569 3023 830
Size: 1739 454 3478
Fragments arranged by size:
3478 1739 454
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5671
SalI G'TCGA_C
Cuts at: 1936 3013 1936
Size: 1077 4594
SbfI CC_TGCA'GG
Cuts at: 3023 3023
Size: 5671
ScaI AGT'ACT
Cuts at: 3919 5631 3919
Size: 1712 3959
SfcI C'TryA_G
Cuts at: 826 1045 2565 2949 3019 4154 4835 5026 826
Size: 219 1520 384 70 1135 681 191 1471
Fragments arranged by size:
1520 1471 1135 681 384 219 191 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5671
SmaI CCC'GGG
Cuts at: 1872 2998 1872
Size: 1126 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3775 4676 4938 820
Size: 197 926 1832 901 262 1553
Fragments arranged by size:
1832 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3462 493
Size: 2969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5671
SphI G_CATG'C
Cuts at: 1132 3029 1132
Size: 1897 3774
SspI AAT'ATT
Cuts at: 5 52 3595 5
Size: 47 3543 2081
Fragments arranged by size:
3543 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5671
StyI C'CwwG_G
Cuts at: 513 1065 2257 3110 513
Size: 552 1192 853 3074
Fragments arranged by size:
3074 1192 853 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2355 2952 3698 3715 3868 4053 4498
Size: 1255 775 597 746 17 153 185 445
Cuts at: 4498 325
Size: 1498
Fragments arranged by size:
1498 1255 775 746 597 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2596 3917 5629
Size: 274 80 33 51 1443 619 1321 1712
Cuts at: 5629 96
Size: 138
Fragments arranged by size:
1712 1443 1321 619 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5631 5663 5631
Size: 32 5639
Tth111I GACn'n_nGTC
Cuts at: 1316 2835 1316
Size: 1519 4152
XbaI T'CTAG_A
Cuts at: 3007 3007
Size: 5671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2186 2186
Size: 5671
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5671
XmaI C'CCGG_G
Cuts at: 1870 2996 1870
Size: 1126 4545
XmnI GAAnn'nnTTC
Cuts at: 3800 3800
Size: 5671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3479 276
Size: 53 83 186 936 1945 2468
Fragments arranged by size:
2468 1945 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI