Restriction map for
pFN21AE4310
(Circular) (Six-base) MAPSORT of: pFN21AE4310.seq Check: 7996 from: 1 to: 5668
pFN21AE4310 5668 bp
With 182 enzymes: SgfI *
March 28, 2012 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 2233
Size: 5668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2989 2989
Size: 5668
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2028 2234 3731 5115 5383 1632
Size: 396 206 1497 1384 268 1917
Fragments arranged by size:
1917 1497 1384 396 268 206
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5471
AflIII A'CryG_T
Cuts at: 1203 2847 4567 5544 1203
Size: 1644 1720 977 1327
Fragments arranged by size:
1720 1644 1327 977
AgeI A'CCGG_T
Cuts at: 4499 4499
Size: 5668
AhdI GACnn_n'nnGTC
Cuts at: 2215 4397 2215
Size: 2182 3486
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2077 2542 3411 4983 2077
Size: 465 869 1572 2762
Fragments arranged by size:
2762 1572 869 465
ApaLI G'TGCA_C
Cuts at: 3725 4881 3725
Size: 1156 4512
ApoI r'AATT_y
Cuts at: 1493 2585 2977 3203 3355 5464 1493
Size: 1092 392 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 392 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2832 2993 1870
Size: 73 889 161 4545
Fragments arranged by size:
4545 889 161 73
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2102 2795 2989 4444 618
Size: 325 217 627 315 693 194 1455 1842
Fragments arranged by size:
1842 1455 693 627 325 315 217 194
BanII G_rGCy'C
Cuts at: 1391 2100 2987 1391
Size: 709 887 4072
Fragments arranged by size:
4072 887 709
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 928
Size: 971 219 4478
Fragments arranged by size:
4478 971 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3859 3893 1903
Size: 34 1922 34 3678
Fragments arranged by size:
3678 1922 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4279 136
Size: 107 122 71 631 605 2607 1525
Fragments arranged by size:
2607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2798 2866 3729 4885 1790
Size: 1008 68 863 1156 2573
Fragments arranged by size:
2573 1156 1008 863 68
BmgBI CAC'GTC
Cuts at: 1318 2284 1318
Size: 966 4702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3399 4357 442
Size: 416 53 83 399 24 1982 958 1753
Fragments arranged by size:
1982 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2574 2943 4328 1239
Size: 1335 369 1385 2579
Fragments arranged by size:
2579 1385 1335 369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3793 4658 4956 3793
Size: 865 298 4505
Fragments arranged by size:
4505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4331 882
Size: 617 2832 2219
Fragments arranged by size:
2832 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3459 493
Size: 711 2255 2702
Fragments arranged by size:
2702 2255 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 2264 3475 3857
Size: 53 83 186 936 582 149 1211 382
Cuts at: 3857 4492 275
Size: 635 1451
Fragments arranged by size:
1451 1211 936 635 582 382 186 149
83 53
BsaWI w'CCGG_w
Cuts at: 4101 4499 4773 4920 5331 4101
Size: 398 274 147 411 4438
Fragments arranged by size:
4438 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2016 2046 2952 2982 1178
Size: 30 165 30 613 30 906 30 3864
Fragments arranged by size:
3864 906 613 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 2973 1186
Size: 1195 592 3881
Fragments arranged by size:
3881 1195 592
BseYI C'CCAG_C
Cuts at: 2151 4871 5367 2151
Size: 2720 496 2452
Fragments arranged by size:
2720 2452 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5095
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3118 3879 4907 1540
Size: 275 186 1117 761 1028 2301
Fragments arranged by size:
2301 1117 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 3729 3814 4885 2186
Size: 1543 85 1071 2969
Fragments arranged by size:
2969 1543 1071 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2186 2798 2866 2987 3729 3814
Size: 399 310 86 612 68 121 742 85
Cuts at: 3814 4885 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 310 121 86
85 68
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 3023 844
Size: 1389 790 3489
Fragments arranged by size:
3489 1389 790
BsrBI CCG'CTC
Cuts at: 1394 1823 3555 1394
Size: 429 1732 3507
Fragments arranged by size:
3507 1732 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2315 4312 4499 1700
Size: 110 505 1997 187 2869
Fragments arranged by size:
2869 1997 505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5668
BssSI C'ACGA_G
Cuts at: 2519 3728 4740 2519
Size: 1209 1012 3447
Fragments arranged by size:
3447 1209 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2293 2759 794
Size: 1499 466 3703
Fragments arranged by size:
3703 1499 466
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2343 2370 2998 3365 3750 3767 5219 5305
Size: 371 27 628 367 385 17 1452 86
Cuts at: 5305 5317 5663 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 628 385 371 367 346 86
27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2078 2364 2078
Size: 286 5382
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2058 2268 513
Size: 552 939 6 48 210 3913
Fragments arranged by size:
3913 939 552 210 48 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 3184 3978 3998 3184
Size: 794 20 4854
Fragments arranged by size:
4854 794 20
ClaI AT'CG_AT
Cuts at: 1554 2780 1554
Size: 1226 4442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3115 4004
Size: 54 1425 50 275 18 177 1108 889
Cuts at: 4004 5633 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1108 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3115 1537
Size: 275 1303 4090
Fragments arranged by size:
4090 1303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3598 1353
Size: 2245 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2028 2234 2574 2943 3731 4328 5115
Size: 393 396 206 340 369 788 597 787
Cuts at: 5115 5383 1239
Size: 268 1524
Fragments arranged by size:
1524 788 787 597 396 393 369 340
268 206
EcoNI CCTnn'n_nnAGG
Cuts at: 2081 2546 2081
Size: 465 5203
EcoO109I rG'GnC_Cy
Cuts at: 2539 2539
Size: 5668
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
EcoRV GAT'ATC
Cuts at: 2655 2857 2655
Size: 202 5466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2960 2960
Size: 5668
FspI TGC'GCA
Cuts at: 4174 4174
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2158 4815 1678
Size: 480 2657 2531
Fragments arranged by size:
2657 2531 480
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2016 2048 2201 2233 2417
Size: 32 135 32 444 32 153 32 184
Cuts at: 2417 2449 2815 2847 4309 4341 4383 4415 1373
Size: 32 366 32 1462 32 42 32 2626
Fragments arranged by size:
2626 1462 444 366 184 153 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KpnI G_GTAC'C
Cuts at: 2993 2993
Size: 5668
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2832 4782 4966 1305
Size: 1527 1950 184 2007
Fragments arranged by size:
2007 1950 1527 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3669
Fragments arranged by size:
3669 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2819 3626 3985 4144 518
Size: 648 843 810 807 359 159 2042
Fragments arranged by size:
2042 843 810 807 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2480 3084 3761 4909 5154 1606
Size: 874 604 677 1148 245 2120
Fragments arranged by size:
2120 1148 874 677 604 245
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 5668
NcoI C'CATG_G
Cuts at: 513 1065 2004 2010 2058 513
Size: 552 939 6 48 4123
Fragments arranged by size:
4123 939 552 48 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5668
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 5668
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 2633 2851 3026 4571 1132
Size: 97 1404 218 175 1545 2229
Fragments arranged by size:
2229 1545 1404 218 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 2847 4567 2847
Size: 1720 3948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PfoI T'CCnGG_A
Cuts at: 2354 2606 2354
Size: 252 5416
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2539 2539
Size: 5668
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2931 2963 2931
Size: 32 5636
PstI C_TGCA'G
Cuts at: 830 3020 830
Size: 2190 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
PvuII CAG'CTG
Cuts at: 2480 2480
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 3020 3020
Size: 5668
ScaI AGT'ACT
Cuts at: 2940 3916 5628 2940
Size: 976 1712 2980
Fragments arranged by size:
2980 1712 976
SfcI C'TryA_G
Cuts at: 826 1045 2724 2929 3016 4151 4832 5023 826
Size: 219 1679 205 87 1135 681 191 1471
Fragments arranged by size:
1679 1471 1135 681 219 205 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2995 1872
Size: 1123 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2832 3772 4673 4935 820
Size: 197 926 889 940 901 262 1553
Fragments arranged by size:
1553 940 926 901 889 262 197
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2058 3107 513
Size: 552 939 6 48 1049 3074
Fragments arranged by size:
3074 1049 939 552 48 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 2938 3914
Size: 274 80 33 51 1443 918 43 976
Cuts at: 3914 5626 96
Size: 1712 138
Fragments arranged by size:
1712 1443 976 918 274 138 80 51
43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5628 5660 5628
Size: 32 5636
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2402
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 2832 1943
Size: 889 4779
XmaI C'CCGG_G
Cuts at: 1870 2993 1870
Size: 1123 4545
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI